RiprA.00162.b

DNA gyrase subunit B

CENTER ID: RiprA.00162.b
ORGANISM: Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE: Endemic typhus
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiprA.00162.b.B1.GE41041 Full length(RiprA.00162.b) 1 807
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272947.5.peg.597
UniProt: Q9ZCX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVIEEKCNE SSYSADSIKV LKGLEAVRKR PGMYIGDVGD GSGLHHMIYE VVDNSIDEAL AGYCDLVQVT LNKNGSVTVS DNGRGIPVEI HEEEGISAAE VIMTQLHAGG KFDQQSYKIS GGLHGVGVSV VNALSEWLEL RIWRNNKEYF IRFNNGITEA PLSIVKENID KKGTEVTFFP SVGTFTNIEF DFVTIEHRLR ELAFLNSGVK ILLVDNRFEE VKKVEFYYTG GIEAYVQYID RAKHAIHPCI VVNTVHVESG ISLELALHWN DSYHENILCF TNNIRQRDGG THLSAFKSAI TRVITSYLDT TGLNKKTKHD FSGEDTREGI CCVLSVKVPD PKFSSQTKDK LVSSEVRPVV ENAVYTKVLE WFEEHPTEAK AIIAKIMEAA NAREAARKAR ELTRRKSALE VSNLPGKLAD CHAKDPAISE LFIVEGDSAG GTAKQGRDSK IQAILPLRGK ILNVERARFD KMLGSDQIGT LITALGISVE REFSLEKLRY HKVIIMTDAD VDGSHIRALL LTFFYRHMPE LINKGYLYIA QPPLYKVKSG ASELYLKNEK ALQNYLIKST INDTYLILDG QEQLVGENLE DLINKVVKFN NLLDHVSKKF NRSITEILAI NDLFNKKIFE PESSARLQKA LDILNNLEES PDKTNLQVLK HENKIEFFHF SRGLKETKIL LKEQLELFEF VEISQFALSI FDIFSKRLKL IVKSKAFDIL TPSQLLNTII ECGKKGITIQ RFKGLGEMNS DQLWETTLDP TKRTLLQVRV AEVDEAEGIF STLMGDVVEP RRLFIQANAL NVMNLDV
NT Sequence
ATGTCGGTAA TTGAAGAAAA ATGTAATGAA TCATCATATA GTGCTGATTC TATAAAGGTA TTAAAAGGTC TTGAAGCAGT AAGAAAAAGA CCAGGAATGT ACATAGGTGA TGTTGGAGAT GGATCTGGTC TACATCATAT GATTTATGAA GTAGTTGACA ATTCTATCGA TGAAGCGCTT GCTGGTTATT GTGATTTAGT ACAAGTAACA TTAAATAAAA ATGGTTCAGT AACCGTGTCT GATAATGGAC GAGGTATACC TGTTGAAATT CATGAAGAAG AAGGGATATC GGCAGCTGAA GTAATTATGA CGCAGCTTCA TGCGGGTGGT AAGTTTGATC AGCAGTCCTA CAAGATTTCA GGCGGTCTGC ATGGTGTTGG TGTATCGGTA GTAAATGCAC TTTCTGAGTG GCTTGAGCTG CGTATTTGGC GTAATAATAA AGAATATTTT ATTAGATTTA ATAATGGTAT AACTGAAGCT CCACTTTCAA TTGTCAAAGA AAATATTGAT AAAAAAGGTA CAGAAGTTAC TTTTTTCCCA TCTGTAGGTA CATTCACTAA TATAGAATTT GATTTTGTGA CTATAGAACA TCGTCTTCGA GAGCTTGCAT TTTTAAATTC AGGCGTTAAG ATTTTACTCG TTGATAATCG CTTTGAAGAA GTTAAAAAAG TAGAGTTTTA TTATACAGGT GGTATAGAAG CTTATGTACA ATATATAGAC AGAGCGAAGC ATGCTATTCA TCCATGTATA GTAGTAAATA CTGTGCATGT TGAGTCAGGT ATAAGCCTTG AGCTTGCACT TCATTGGAAT GATTCTTATC ATGAGAATAT TCTATGTTTC ACTAATAATA TTAGGCAGCG TGACGGTGGG ACTCATCTTA GTGCTTTCAA ATCAGCGATT ACACGTGTTA TCACTTCTTA TCTAGATACT ACAGGTCTTA ATAAAAAAAC TAAACATGAT TTTAGTGGTG AAGATACTAG AGAAGGGATT TGCTGTGTAT TATCTGTTAA AGTCCCTGAT CCTAAATTTT CTTCTCAAAC AAAAGATAAG CTAGTAAGTT CTGAAGTACG ACCTGTTGTA GAGAATGCCG TTTATACAAA GGTTCTTGAG TGGTTTGAAG AACATCCTAC TGAAGCTAAG GCAATTATAG CTAAGATTAT GGAAGCTGCC AATGCTCGTG AAGCTGCTAG AAAAGCAAGA GAACTTACTC GAAGAAAATC AGCTTTAGAA GTTTCAAATT TACCAGGTAA ACTTGCAGAT TGCCATGCCA AAGATCCTGC TATTTCAGAA TTGTTTATTG TTGAGGGGGA CTCTGCGGGT GGTACTGCAA AGCAGGGAAG GGATAGCAAA ATTCAGGCTA TACTGCCTTT GCGTGGTAAA ATTTTGAATG TTGAACGAGC AAGATTTGAT AAAATGCTTG GTTCGGACCA AATTGGTACA TTAATTACAG CTCTTGGGAT TAGTGTTGAA CGAGAATTTT CTTTAGAAAA GCTGAGATAC CATAAAGTTA TTATTATGAC CGATGCTGAT GTTGATGGTT CGCATATTAG AGCTTTGTTG CTCACATTTT TTTATCGTCA TATGCCAGAG CTTATAAATA AAGGTTATCT ATATATAGCA CAGCCACCGC TTTATAAGGT AAAAAGTGGT GCATCTGAGC TTTATTTAAA GAATGAAAAA GCTTTGCAAA ATTATTTAAT AAAATCAACT ATTAATGATA CATACTTAAT TCTAGACGGT CAAGAGCAGT TAGTAGGTGA GAATTTAGAA GATCTAATTA ATAAAGTAGT TAAGTTTAAC AATTTGCTTG ATCATGTTAG TAAAAAATTT AATCGCTCAA TTACTGAAAT TCTTGCAATT AATGACTTAT TTAATAAAAA AATATTTGAA CCTGAAAGTA GTGCAAGGCT TCAAAAAGCT TTAGATATTT TAAATAATTT AGAGGAGTCA CCAGATAAAA CTAATTTGCA AGTGTTAAAA CATGAGAATA AAATAGAATT TTTTCATTTT AGTAGAGGTT TAAAAGAAAC TAAAATTTTG TTAAAAGAGC AGTTAGAATT ATTTGAATTT GTAGAAATAT CACAGTTTGC TTTATCAATT TTTGATATAT TTAGTAAGCG ATTAAAGCTT ATAGTAAAAA GTAAGGCATT TGATATTTTA ACCCCAAGCC AGTTGTTAAA TACTATTATA GAATGCGGTA AAAAAGGTAT AACTATTCAG CGTTTTAAAG GTCTTGGAGA GATGAATTCC GATCAGCTGT GGGAAACTAC TCTTGATCCT ACAAAGCGAA CGTTGCTTCA AGTAAGAGTT GCTGAAGTAG ATGAAGCAGA GGGGATTTTT TCTACTTTAA TGGGTGATGT TGTTGAGCCT CGTAGATTAT TTATCCAAGC TAATGCTTTA AATGTTATGA ATTTAGATGT A
Details for RiprA.00162.b.B1.GE41041
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 92kDa
OBSERVED MW: 92kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS VIEEKCNESS YSADSIKVLK GLEAVRKRPG MYIGDVGDGS GLHHMIYEVV DNSIDEALAG YCDLVQVTLN KNGSVTVSDN GRGIPVEIHE EEGISAAEVI MTQLHAGGKF DQQSYKISGG LHGVGVSVVN ALSEWLELRI WRNNKEYFIR FNNGITEAPL SIVKENIDKK GTEVTFFPSV GTFTNIEFDF VTIEHRLREL AFLNSGVKIL LVDNRFEEVK KVEFYYTGGI EAYVQYIDRA KHAIHPCIVV NTVHVESGIS LELALHWNDS YHENILCFTN NIRQRDGGTH LSAFKSAITR VITSYLDTTG LNKKTKHDFS GEDTREGICC VLSVKVPDPK FSSQTKDKLV SSEVRPVVEN AVYTKVLEWF EEHPTEAKAI IAKIMEAANA REAARKAREL TRRKSALEVS NLPGKLADCH AKDPAISELF IVEGDSAGGT AKQGRDSKIQ AILPLRGKIL NVERARFDKM LGSDQIGTLI TALGISVERE FSLEKLRYHK VIIMTDADVD GSHIRALLLT FFYRHMPELI NKGYLYIAQP PLYKVKSGAS ELYLKNEKAL QNYLIKSTIN DTYLILDGQE QLVGENLEDL INKVVKFNNL LDHVSKKFNR SITEILAIND LFNKKIFEPE SSARLQKALD ILNNLEESPD KTNLQVLKHE NKIEFFHFSR GLKETKILLK EQLELFEFVE ISQFALSIFD IFSKRLKLIV KSKAFDILTP SQLLNTIIEC GKKGITIQRF KGLGEMNSDQ LWETTLDPTK RTLLQVRVAE VDEAEGIFST LMGDVVEPRR LFIQANALNV MNLDV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgg taattgaaga aaaatgtaat gaatcatcat atagtgctga ttctataaag gtattaaaag gtcttgaagc agtaagaaaa agaccaggaa tgtacatagg tgatgttgga gatggatctg gtctacatca tatgatttat gaagtagttg acaattctat cgatgaagcg cttgctggtt attgtgattt agtacaagta acattaaata aaaatggttc agtaaccgtg tctgataatg gacgaggtat acctgttgaa attcatgaag aagaagggat atcggcagct gaagtaatta tgacgcagct tcatgcgggt ggtaagtttg atcagcagtc ctacaagatt tcaggcggtc tgcatggtgt tggtgtatcg gtagtaaatg cactttctga gtggcttgag ctgcgtattt ggcgtaataa taaagaatat tttattagat ttaataatgg tataactgaa gctccacttt caattgtcaa agaaaatatt gataaaaaag gtacagaagt tacttttttc ccatctgtag gtacattcac taatatagaa tttgattttg tgactataga acatcgtctt cgagagcttg catttttaaa ttcaggcgtt aagattttac tcgttgataa tcgctttgaa gaagttaaaa aagtagagtt ttattataca ggtggtatag aagcttatgt acaatatata gacagagcga agcatgctat tcatccatgt atagtagtaa atactgtgca tgttgagtca ggtataagcc ttgagcttgc acttcattgg aatgattctt atcatgagaa tattctatgt ttcactaata atattaggca gcgtgacggt gggactcatc ttagtgcttt caaatcagcg attacacgtg ttatcacttc ttatctagat actacaggtc ttaataaaaa aactaaacat gattttagtg gtgaagatac tagagaaggg atttgctgtg tattatctgt taaagtccct gatcctaaat tttcttctca aacaaaagat aagctagtaa gttctgaagt acgacctgtt gtagagaatg ccgtttatac aaaggttctt gagtggtttg aagaacatcc tactgaagct aaggcaatta tagctaagat tatggaagct gccaatgctc gtgaagctgc tagaaaagca agagaactta ctcgaagaaa atcagcttta gaagtttcaa atttaccagg taaacttgca gattgccatg ccaaagatcc tgctatttca gaattgttta ttgttgaggg ggactctgcg ggtggtactg caaagcaggg aagggatagc aaaattcagg ctatactgcc tttgcgtggt aaaattttga atgttgaacg agcaagattt gataaaatgc ttggttcgga ccaaattggt acattaatta cagctcttgg gattagtgtt gaacgagaat tttctttaga aaagctgaga taccataaag ttattattat gaccgatgct gatgttgatg gttcgcatat tagagctttg ttgctcacat ttttttatcg tcatatgcca gagcttataa ataaaggtta tctatatata gcacagccac cgctttataa ggtaaaaagt ggtgcatctg agctttattt aaagaatgaa aaagctttgc aaaattattt aataaaatca actattaatg atacatactt aattctagac ggtcaagagc agttagtagg tgagaattta gaagatctaa ttaataaagt agttaagttt aacaatttgc ttgatcatgt tagtaaaaaa tttaatcgct caattactga aattcttgca attaatgact tatttaataa aaaaatattt gaacctgaaa gtagtgcaag gcttcaaaaa gctttagata ttttaaataa tttagaggag tcaccagata aaactaattt gcaagtgtta aaacatgaga ataaaataga attttttcat tttagtagag gtttaaaaga aactaaaatt ttgttaaaag agcagttaga attatttgaa tttgtagaaa tatcacagtt tgctttatca atttttgata tatttagtaa gcgattaaag cttatagtaa aaagtaaggc atttgatatt ttaaccccaa gccagttgtt aaatactatt atagaatgcg gtaaaaaagg tataactatt cagcgtttta aaggtcttgg agagatgaat tccgatcagc tgtgggaaac tactcttgat cctacaaagc gaacgttgct tcaagtaaga gttgctgaag tagatgaagc agaggggatt ttttctactt taatgggtga tgttgttgag cctcgtagat tatttatcca agctaatgct ttaaatgtta tgaatttaga tgtatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat