RiprA.00148.b

site-specific tyrosine recombinase XerC

CENTER ID:	RiprA.00148.b
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00148.b.B1.GE27185	Full length( RiprA.00148.b )		1	305

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_137884
PATRIC ID:	fig\|272947.5.peg.852
RefSeq:	NP_221166.1
UniProt:	Q9ZCE0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLDISIQELI KQWQKYLILQ KNYSNNTVIA YNNDLKHFLE FMNYYNSELV TLNHIKTADI RLIRSWLAKR KCENFTASSI ARGLSTVKNF YKFLEKTLLL NNHIIFSIKS PKKAKLLPKG LSVDDVLISL EHIEEYGNVK WVELRNKALI VLIYAAGLRI SEALSITKLH LQNLEFIKII GKGSKERIIP WLPLTKNLIT KYLEILPYKL DENEPIFRGR QGKKLQPSVF NRELIKLKRI YGLPEYLTAH SFRHSFASHL LEYGADLRSI QELLGHKSLS TTQKYTQTSI KHLEAVYNTA YPIKK

NT Sequence

ATGTTAGATA TATCAATTCA AGAATTAATA AAACAGTGGC AAAAATATCT CATTTTGCAA AAAAATTACT CTAATAATAC AGTAATTGCT TATAATAATG ATCTTAAGCA TTTCCTTGAG TTTATGAATT ATTATAACTC AGAGCTTGTA ACACTCAATC ATATTAAAAC TGCTGATATA AGACTAATAC GTAGTTGGCT TGCAAAGCGT AAATGTGAAA ATTTTACTGC TTCTTCAATT GCACGTGGTT TATCTACAGT AAAAAATTTT TATAAGTTTT TAGAAAAAAC ACTATTATTA AATAATCATA TAATTTTTTC TATAAAATCT CCTAAAAAGG CTAAATTGTT ACCAAAAGGT TTATCAGTAG ATGATGTATT AATATCACTT GAACATATTG AAGAATACGG CAATGTTAAG TGGGTAGAAC TTAGAAATAA AGCCCTAATT GTTCTTATAT ATGCTGCAGG TCTACGTATC TCAGAAGCAT TATCAATTAC AAAGCTTCAT CTGCAAAATC TAGAATTTAT AAAAATAATA GGTAAAGGTA GTAAAGAAAG AATAATCCCG TGGTTACCTC TTACTAAGAA TTTAATTACC AAATATTTAG AAATATTACC GTATAAGTTA GATGAGAATG AACCTATATT TAGAGGAAGA CAGGGTAAAA AATTACAACC GTCGGTATTT AATCGTGAAT TAATTAAGTT AAAGCGTATC TATGGGTTAC CTGAATATTT AACTGCTCAT TCATTTAGAC ATAGTTTTGC TTCACATTTA CTTGAGTATG GTGCAGATTT ACGTTCTATT CAAGAGCTAT TAGGTCATAA AAGTCTATCT ACTACACAAA AATATACTCA AACAAGTATA AAACATTTAG AGGCTGTATA TAATACAGCA TATCCAATTA AAAAA

Details for RiprA.00148.b.B1.GE27185

HARVESTED ON:	8/17/2009
SEQUENCED ON:	9/29/2009
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML DISIQELIKQ WQKYLILQKN YSNNTVIAYN NDLKHFLEFM NYYNSELVTL NHIKTADIRL IRSWLAKRKC ENFTASSIAR GLSTVKNFYK FLEKTLLLNN HIIFSIKSPK KAKLLPKGLS VDDVLISLEH IEEYGNVKWV ELRNKALIVL IYAAGLRISE ALSITKLHLQ NLEFIKIIGK GSKERIIPWL PLTKNLITKY LEILPYKLDE NEPIFRGRQG KKLQPSVFNR ELIKLKRIYG LPEYLTAHSF RHSFASHLLE YGADLRSIQE LLGHKSLSTT QKYTQTSIKH LEAVYNTAYP IKK

Validated NT Sequence

cctatcttac tcacttattt tttaattgga tatgctgtat tatatacagc ctctaaatgt tttatacttg tttgagtata tttttgtgta gtagatagac ttttatgacc taatagctct tgaatagaac gtaaatctgc accatactca agtaaatgtg aagcaaaact atgtctaaat gaatgagcag ttaaatattc aggtaaccca tagatacgct ttaacttaat taattcacga ttaaataccg acggttgtaa ttttttaccc tgtcttcctc taaatatagg ttcattctca tctaacttat acggtaatat ttctaaatat ttggtaatta aattcttagt aagaggtaac cacgggatta ttctttcttt actaccttta cctattattt ttataaattc tagattttgc agatgaagct ttgtaattga taatgcttct gagatacgta gacctgcagc atatataaga acaattaggg ctttatttct aagttctacc cacttaacat tgccgtattc ttcaatatgt tcaagtgata ttaatacatc atctactgat aaaccttttg gtaacaattt agccttttta ggagatttta tagaaaaaat tatatgatta tttaataata gtgttttttc taaaaactta taaaaatttt ttactgtaga taaaccacgt gcaattgaag aagcagtaaa attttcacat ttacgctttg caagccaact acgtattagt cttatatcag cagttttaat atgattgagt gttacaagct ctgagttata ataattcata aactcaagga aatgcttaag atcattatta taagcaatta ctgtattatt agagtaattt ttttgcaaaa tgagatattt ttgccactgt tttattaatt cttgaattga tatatctaac atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttag atatatcaat tcaagaatta ataaaacagt ggcaaaaata tctcattttg caaaaaaatt actctaataa tacagtaatt gcttataata atgatcttaa gcatttcctt gagtttatga attattataa ctcagagctt gtaacactca atcatattaa aactgctgat ataagactaa tacgtagttg gcttgcaaag cgtaaatgtg aaaattttac tgcttcttca attgcacgtg gtttatctac agtaaaaaat ttttataagt ttttagaaaa aacactatta ttaaataatc atataatttt ttctataaaa tctcctaaaa aggctaaatt gttaccaaaa ggtttatcag tagatgatgt attaatatca cttgaacata ttgaagaata cggcaatgtt aagtgggtag aacttagaaa taaagcccta attgttctta tatatgctgc aggtctacgt atctcagaag cattatcaat tacaaagctt catctgcaaa atctagaatt tataaaaata ataggtaaag gtagtaaaga aagaataatc ccgtggttac ctcttactaa gaatttaatt accaaatatt tagaaatatt accgtataag ttagatgaga atgaacctat atttagagga agacagggta aaaaattaca accgtcggta tttaatcgtg aattaattaa gttaaagcgt atctatgggt tacctgaata tttaactgct cattcattta gacatagttt tgcttcacat ttacttgagt atggtgcaga tttacgttct attcaagagc tattaggtca taaaagtcta tctactacac aaaaatatac tcaaacaagt ataaaacatt tagaggctgt atataataca gcatatccaa ttaaaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat