RiprA.00135.a

queuine tRNA-ribosyltransferase

CENTER ID: RiprA.00135.a
ORGANISM: Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE: Endemic typhus
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
RiprA.00135.a.A4.GE28247 Created from Decode construct for feature RiprA.00135.a 1 354
RiprA.00135.a.A5.GE28248 Created from Decode construct for feature RiprA.00135.a 4 361
RiprA.00135.a.A6.GE28249 Created from Decode construct for feature RiprA.00135.a 4 354
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126946
PATRIC ID: fig|272947.5.peg.756
RefSeq: NP_221076
UniProt: Q9ZCK8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKFSFTIHS HYKKARSGVI TTAHGEIRTP AFMPVGTRGA VKAMLTEAVV ETGADILLGN TYHLMLQPSA ERIAYLGGLH KFMNWDKPIL TDSGGFQVMS LSKLCKITEE GVSFRSHING NKYMLTPEYS TEIQYLLGST ITMALDECTP YPSTFEKAKT SMHLTTRWAN RSRDAFVKRE GYAQFGIIQG SVYKELREQS VKDLVKCDFE GYAIGGLAVG EGQELMFRVL DYVPDFLPQN KPRYLMGVGK PSDIIGAVSR GIDMFDCVIP TRSGRNGQAF TKYGTVNIRN SKYADDKEPL EHDCKCPACT NYTKAYLHHL VRIREILGPM LMTWHNLTYF QNLMSRIRTY IKLGKDFDFV H
NT Sequence
GTGTCAAAAT TTTCGTTTAC TATTCATAGT CACTATAAAA AAGCTAGAAG CGGTGTGATT ACCACTGCTC ATGGTGAAAT TCGTACACCA GCTTTTATGC CGGTTGGTAC TAGAGGTGCA GTTAAGGCAA TGTTGACTGA AGCAGTAGTT GAGACCGGAG CAGATATATT ACTCGGCAAT ACTTATCACT TAATGTTGCA GCCAAGTGCT GAGCGTATAG CATATCTTGG TGGTTTGCAT AAATTTATGA ATTGGGATAA GCCTATACTA ACTGATTCCG GTGGTTTTCA GGTAATGTCC TTATCAAAGT TATGCAAGAT AACTGAAGAG GGAGTAAGTT TCAGATCTCA TATTAACGGT AATAAATATA TGTTAACGCC TGAGTATTCG ACTGAAATAC AATATCTACT TGGTAGTACA ATCACTATGG CTCTTGATGA ATGCACTCCT TATCCTTCAA CTTTTGAAAA AGCTAAAACT TCTATGCATC TTACGACTAG ATGGGCTAAT AGATCACGTG ATGCTTTTGT TAAGCGCGAA GGTTATGCGC AATTTGGTAT TATTCAGGGA AGTGTTTATA AAGAATTGCG TGAACAATCG GTGAAAGATT TAGTAAAATG CGATTTTGAG GGTTATGCTA TAGGAGGTCT TGCAGTAGGT GAGGGGCAGG AACTTATGTT TAGAGTTCTT GATTATGTTC CTGATTTTTT GCCGCAAAAT AAACCACGTT ATTTAATGGG TGTGGGGAAG CCTTCTGATA TTATTGGTGC AGTTAGTAGA GGTATAGATA TGTTTGACTG TGTAATCCCA ACTCGTTCAG GTCGCAATGG TCAGGCTTTT ACAAAATATG GTACAGTTAA TATACGCAAT AGCAAATATG CTGATGATAA AGAGCCACTT GAGCATGATT GTAAATGTCC TGCTTGCACT AATTACACGA AAGCTTATTT GCATCATTTA GTTAGAATCC GTGAGATACT TGGTCCTATG CTAATGACAT GGCATAATTT AACATATTTT CAAAATCTTA TGAGTCGTAT TAGAACATAT ATTAAACTAG GTAAAGATTT CGATTTTGTA CAT
Details for RiprA.00135.a.A4.GE28247
HARVESTED ON: 2/2/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSKFSFTI HSHYKKARSG VITTAHGEIR TPAFMPVGTR GAVKAMLTEA VVETGADILL GNTYHLMLQP SAERIAYLGG LHKFMNWDKP ILTDSGGFQV MSLSKLCKIT EEGVSFRSHI NGNKYMLTPE YSTEIQYLLG STITMALDEC TPYPSTFEKA KTSMHLTTRW ANRSRDAFVK REGYAQFGII QGSVYKELRE QSVKDLVKCD FEGYAIGGLA VGEGQELMFR VLDYVPDFLP QNKPRYLMGV GKPSDIIGAV SRGIDMFDCV IPTRSGRNGQ AFTKYGTVNI RNSKYADDKE PLEHDCKCPA CTNYTKAYLH HLVRIREILG PMLMTWHNLT YFQNLMSRIR TYIKLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcaaaa ttttcgttta ctattcatag tcactataaa aaagctagaa gcggtgtgat taccactgct catggtgaaa ttcgtacacc agcttttatg ccggttggta ctagaggtgc agttaaggca atgttgactg aagcagtagt tgagaccgga gcagatatat tactcggcaa tacttatcac ttaatgttgc agccaagtgc tgagcgtata gcatatcttg gtggtttgca taaatttatg aattgggata agcctatact aactgattcc ggtggttttc aggtaatgtc cttatcaaag ttatgcaaga taactgaaga gggagtaagt ttcagatctc atattaacgg taataaatat atgttaacgc ctgagtattc gactgaaata caatatctac ttggtagtac aatcactatg gctcttgatg aatgcactcc ttatccttca acttttgaaa aagctaaaac ttctatgcat cttacgacta gatgggctaa tagatcacgt gatgcttttg ttaagcgcga aggttatgcg caatttggta ttattcaggg aagtgtttat aaagaattgc gtgaacaatc ggtgaaagat ttagtaaaat gcgattttga gggttatgct ataggaggtc ttgcagtagg tgaggggcag gaacttatgt ttagagttct tgattatgtt cctgattttt tgccgcaaaa taaaccacgt tatttaatgg gtgtggggaa gccttctgat attattggtg cagttagtag aggtatagat atgtttgact gtgtaatccc aactcgttca ggtcgcaatg gtcaggcttt tacaaaatat ggtacagtta atatacgcaa tagcaaatat gctgatgata aagagccact tgagcatgat tgtaaatgtc ctgcttgcac taattacacg aaagcttatt tgcatcattt agttagaatc cgtgagatac ttggtcctat gctaatgaca tggcataatt taacatattt tcaaaatctt atgagtcgta ttagaacata tattaaacta ggtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for RiprA.00135.a.A5.GE28248
HARVESTED ON: 2/2/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFSFTIHSH YKKARSGVIT TAHGEIRTPA FMPVGTRGAV KAMLTEAVVE TGADILLGNT YHLMLQPSAE RIAYLGGLHK FMNWDKPILT DSGGFQVMSL SKLCKITEEG VSFRSHINGN KYMLTPEYST EIQYLLGSTI TMALDECTPY PSTFEKAKTS MHLTTRWANR SRDAFVKREG YAQFGIIQGS VYKELREQSV KDLVKCDFEG YAIGGLAVGE GQELMFRVLD YVPDFLPQNK PRYLMGVGKP SDIIGAVSRG IDMFDCVIPT RSGRNGQAFT KYGTVNIRNS KYADDKEPLE HDCKCPACTN YTKAYLHHLV RIREILGPML MTWHNLTYFQ NLMSRIRTYI KLGKDFDFVH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttttcgttt actattcata gtcactataa aaaagctaga agcggtgtga ttaccactgc tcatggtgaa attcgtacac cagcttttat gccggttggt actagaggtg cagttaaggc aatgttgact gaagcagtag ttgagaccgg agcagatata ttactcggca atacttatca cttaatgttg cagccaagtg ctgagcgtat agcatatctt ggtggtttgc ataaatttat gaattgggat aagcctatac taactgattc cggtggtttt caggtaatgt ccttatcaaa gttatgcaag ataactgaag agggagtaag tttcagatct catattaacg gtaataaata tatgttaacg cctgagtatt cgactgaaat acaatatcta cttggtagta caatcactat ggctcttgat gaatgcactc cttatccttc aacttttgaa aaagctaaaa cttctatgca tcttacgact agatgggcta atagatcacg tgatgctttt gttaagcgcg aaggttatgc gcaatttggt attattcagg gaagtgttta taaagaattg cgtgaacaat cggtgaaaga tttagtaaaa tgcgattttg agggttatgc tataggaggt cttgcagtag gtgaggggca ggaacttatg tttagagttc ttgattatgt tcctgatttt ttgccgcaaa ataaaccacg ttatttaatg ggtgtgggga agccttctga tattattggt gcagttagta gaggtataga tatgtttgac tgtgtaatcc caactcgttc aggtcgcaat ggtcaggctt ttacaaaata tggtacagtt aatatacgca atagcaaata tgctgatgat aaagagccac ttgagcatga ttgtaaatgt cctgcttgca ctaattacac gaaagcttat ttgcatcatt tagttagaat ccgtgagata cttggtccta tgctaatgac atggcataat ttaacatatt ttcaaaatct tatgagtcgt attagaacat atattaaact aggtaaagat ttcgattttg tacataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for RiprA.00135.a.A6.GE28249
HARVESTED ON: 2/2/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 56
Validated AA Sequence
MNALLILQTF EKAKTXMHLT TRWANRSRDA XVKREGYAQF GIIQGSVYKE LREQSVKDLV KCDFEGYAIG GLAVGEGQEL MFRVLDYVPD FLPQNKPRYL MGVGKPSDII GAVSRGIDMF DCVIPTRSGR NGQAFTKYGT VNIRNSKYAD DKEPLEHDCK CPACTNYTKA YLHHLVRIRE ILGPMLMTWH NLTYFQNLMS RIRTYIKLG
Validated NT Sequence
gctttgttag cagccggatc ctcgagaagc ttggctgcag aacttgttcg tgctgtttat taacctagtt taatatatgt tctaatacga ctcataagat tttgaaaata tgttaaatta tgccatgtca ttagcatagg accaagtatc tcacggattc taactaaatg atgcaaataa gctttcgtgt aattagtgca agcaggacat ttacaatcat gctcaagtgg ctctttatca tcagcatatt tgctattgcg tatattaact gtaccatatt ttgtaaaagc ctgaccattg cgacctgaac gagttgggat tacacagtca aacatatcta tacctctact aactgcacca ataatatcag aaggcttccc cacacccatt aaataacgtg gtttattttg cggcaaaaaa tcaggaacat aatcaagaac tctaaacata agttcctgcc cctcacctac tgcaagacct cctatagcat aaccctcaaa atcgcatttt actaaatctt tcaccgattg ttcacgcaat tctttataaa cacttccctg aataatacca aattgcgcat aaccttcgcg cttaacnnaa gcatcacgtg atctattagc ccatctagtc gtaagatgca tanaagtttt agctttttca aaagtttgaa ggataaggag tgcattcatc aagagccata gtgattgtac taccaagtag atattgtatt tcagncgaat actcangcgt taacatatnt ttattaccgt taatatgaga tctgaaactt actccctctt cagttatctt gnntaacttt gataaggaca ttacctgaaa nncaccggaa tcagttagta taggcttann nnnatnnata aatnnatgca aaccacnaga tntggcnnna cgctcaacnt ggctgnnnat nanngataan nannnnnnan nannntatcn gctnncggcn cnactactnn ntncnntcna acntnnncnt taannnnnnc tctanncnac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFSFTIHSH YKKARSGVIT TAHGEIRTPA FMPVGTRGAV KAMLTEAVVE TGADILLGNT YHLMLQPSAE RIAYLGGLHK FMNWDKPILT DSGGFQVMSL SKLCKITEEG VSFRSHINGN KYMLTPEYST EIQYLLGSTI TMALDECTPY PSTFEKAKTS MHLTTRWANR SRDAFVKREG YAQFGIIQGS VYKELREQSV KDLVKCDFEG YAIGGLAVGE GQELMFRVLD YVPDFLPQNK PRYLMGVGKP SDIIGAVSRG IDMFDCVIPT RSGRNGQAFT KYGTVNIRNS KYADDKEPLE HDCKCPACTN YTKAYLHHLV RIREILGPML MTWHNLTYFQ NLMSRIRTYI KLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttttcgttt actattcata gtcactataa aaaagctaga agcggtgtga ttaccactgc tcatggtgaa attcgtacac cagcttttat gccggttggt actagaggtg cagttaaggc aatgttgact gaagcagtag ttgagaccgg agcagatata ttactcggca atacttatca cttaatgttg cagccaagtg ctgagcgtat agcatatctt ggtggtttgc ataaatttat gaattgggat aagcctatac taactgattc cggtggtttt caggtaatgt ccttatcaaa gttatgcaag ataactgaag agggagtaag tttcagatct catattaacg gtaataaata tatgttaacg cctgagtatt cgactgaaat acaatatcta cttggtagta caatcactat ggctcttgat gaatgcactc cttatccttc aacttttgaa aaagctaaaa cttctatgca tcttacgact agatgggcta atagatcacg tgatgctttt gttaagcgcg aaggttatgc gcaatttggt attattcagg gaagtgttta taaagaattg cgtgaacaat cggtgaaaga tttagtaaaa tgcgattttg agggttatgc tataggaggt cttgcagtag gtgaggggca ggaacttatg tttagagttc ttgattatgt tcctgatttt ttgccgcaaa ataaaccacg ttatttaatg ggtgtgggga agccttctga tattattggt gcagttagta gaggtataga tatgtttgac tgtgtaatcc caactcgttc aggtcgcaat ggtcaggctt ttacaaaata tggtacagtt aatatacgca atagcaaata tgctgatgat aaagagccac ttgagcatga ttgtaaatgt cctgcttgca ctaattacac gaaagcttat ttgcatcatt tagttagaat ccgtgagata cttggtccta tgctaatgac atggcataat ttaacatatt ttcaaaatct tatgagtcgt attagaacat atattaaact aggtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa