RiprA.00133.a

cysteinyl-tRNA synthetase

CENTER ID:	RiprA.00133.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00133.a.AE1.GE43248	Full length( RiprA.00133.a )		1	457
RiprA.00133.a.B1.GE27187	Full length( RiprA.00133.a )		1	457

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126755
PATRIC ID:	fig\|272947.5.peg.85
RefSeq:	NP_220478.1
UniProt:	Q9ZE62

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLHLYNTLT RTKEVFNPQD PANIKMYVCG PTVYDNPHIG NSRSVVVYDL LYRIIIKIFG KKAVKYVRNI TDVDDKIIDR AELLGITIND LTDKVTREFH INMEYLGCML PTIEPKATEH IDVMIEIIER LIAKDHAYIA DNHVYFDVLS APNYTELSNR NLEAMFEGVR IENSKTKKNP QDFVLWKPAK KNEPENINFT SPWGLGRPGW HIECSAMSYK YLGQNFDIHG GGADLIFPHH TNEIAQSRCA FPSSTYAKYW IHNGFLTVNG SKMSKSLGNF ITVRDLMDKQ IQGEVVRLFL LSSHYRRPLD YNDKAIDDAK KTLDYWYRAI EHINVQKVDL PSNFMRSLFD DMNTPLAIKI INDYAKCVFI SKTEAERKFN ASAIITCANF IGLMRKTQYE WFNRDVDEFY INELINKRLK AKKQKNWLLA DKIRNQLLEK KIILEDKSNY ITIWRKE

NT Sequence

ATGAAACTTC ACTTATATAA CACTCTGACT CGTACTAAAG AGGTTTTTAA TCCTCAAGAT CCTGCTAATA TAAAAATGTA TGTGTGTGGA CCGACTGTTT ATGATAATCC TCATATAGGT AATAGTAGGT CAGTAGTAGT ATATGATTTA CTTTATCGTA TAATTATAAA AATTTTCGGT AAAAAGGCCG TAAAATATGT ACGTAACATC ACTGATGTTG ATGATAAAAT TATTGATAGA GCTGAATTAC TCGGTATTAC TATTAATGAT TTGACTGATA AAGTTACGCG AGAGTTTCAT ATAAATATGG AATATTTAGG TTGCATGTTA CCAACTATTG AGCCTAAAGC TACAGAGCAT ATTGATGTAA TGATCGAAAT AATAGAACGC TTAATAGCAA AAGATCATGC ATATATTGCT GATAATCATG TTTATTTTGA TGTTTTATCA GCTCCAAATT ATACAGAATT ATCTAATCGC AATTTAGAAG CAATGTTTGA GGGAGTTCGT ATAGAAAATA GTAAAACTAA AAAAAACCCA CAGGACTTTG TATTATGGAA GCCAGCAAAG AAAAATGAGC CGGAAAATAT AAATTTTACA AGTCCTTGGG GGCTTGGTCG GCCTGGATGG CATATTGAAT GTTCAGCCAT GAGCTATAAA TATTTAGGGC AAAATTTTGA TATTCATGGA GGTGGAGCAG ATTTAATTTT CCCACATCAT ACCAATGAGA TTGCACAAAG TAGATGTGCT TTTCCAAGTT CTACCTATGC CAAATATTGG ATACATAATG GTTTTTTAAC TGTGAACGGT AGCAAAATGA GCAAATCTCT TGGTAATTTT ATAACAGTAA GGGATTTAAT GGATAAGCAA ATTCAAGGAG AAGTAGTACG ACTATTCTTA TTAAGTAGTC ACTATAGGCG CCCACTCGAT TATAATGATA AAGCTATAGA TGATGCTAAG AAAACTTTAG ATTATTGGTA TCGAGCTATA GAACACATTA ATGTACAAAA AGTAGATTTA CCGTCTAATT TCATGCGAAG CTTATTTGAT GATATGAATA CTCCCCTTGC CATAAAAATA ATCAATGACT ACGCTAAATG TGTTTTTATT TCTAAAACTG AGGCAGAAAG AAAGTTTAAT GCTTCTGCTA TTATTACTTG CGCTAATTTT ATTGGTTTAA TGCGTAAGAC TCAATATGAG TGGTTTAATC GTGATGTTGA TGAATTTTAT ATAAATGAAC TTATAAATAA ACGTTTGAAA GCAAAAAAAC AAAAAAATTG GCTGTTAGCT GATAAAATTC GTAATCAGCT ATTAGAGAAA AAAATAATTC TAGAAGATAA ATCTAACTAT ATTACTATTT GGCGCAAAGA A

Details for RiprA.00133.a.AE1.GE43248

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/18/2019
EXPECTED MW:	54kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gaaacttcac ttatataaca ctctgactcg tactaaagag gtttttaatc ctcaagatcc tgctaatata aaaatgtatg tgtgtggacc gactgtttat gataatcctc atataggtaa tagtaggtca gtagtagtat atgatttact ttatcgtata attataaaaa ttttcggtaa aaaggccgta aaatatgtac gtaacatcac tgatgttgat gataaaatta ttgatagagc tgaattactc ggtattacta ttaatgattt gactgataaa gttacgcgag agtttcatat aaatatggaa tatttaggtt gcatgttacc aactattgag cctaaagcta cagagcatat tgatgtaatg atcgaaataa tagaacgctt aatagcaaaa gatcatgcat atattgctga taatcatgtt tattttgatg ttttatcagc tccaaattat acagaattat ctaatcgcaa tttagaagca atgtttgagg gagttcgtat agaaaatagt aaaactaaaa aaaacccaca ggactttgta ttatggaagc cagcaaagaa aaatgagccg gaaaatataa attttacaag tccttggggg cttggtcggc ctggatggca tattgaatgt tcagccatga gctataaata tttagggcaa aattttgata ttcatggagg tggagcagat ttaattttcc cacatcatac caatgagatt gcacaaagta gatgtgcttt tccaagttct acctatgcca aatattggat acataatggt tttttaactg tgaacggtag caaaatgagc aaatctcttg gtaattttat aacagtaagg gatttaatgg ataagcaaat tcaaggagaa gtagtacgac tattcttatt aagtagtcac tataggcgcc cactcgatta taatgataaa gctatagatg atgctaagaa aactttagat tattggtatc gagctataga acacattaat gtacaaaaag tagatttacc gtctaatttc atgcgaagct tatttgatga tatgaatact ccccttgcca taaaaataat caatgactac gctaaatgtg tttttatttc taaaactgag gcagaaagaa agtttaatgc ttctgctatt attacttgcg ctaattttat tggtttaatg cgtaagactc aatatgagtg gtttaatcgt gatgttgatg aattttatat aaatgaactt ataaataaac gtttgaaagc aaaaaaacaa aaaaattggc tgttagctga taaaattcgt aatcagctat tagagaaaaa aataattcta gaagataaat ctaactatat tactatttgg cgcaaagaag ggcaccacca tcatcatcat taacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat

Details for RiprA.00133.a.B1.GE27187

HARVESTED ON:	8/17/2009
SEQUENCED ON:	9/29/2009
EXPECTED MW:	54kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHXHHHMK LHLYNTLTRT KEVFNPQDPA NIKMYVCGPT VYDNPHIGNS RSVVVYDLLY RIIIKIFGKK AVKYVRNITD VDDKIIDRAE LLGITINDLT DKVTREFHIN MEYLGCMLPT IEPKATEHID VMIEIIERLI AKDHAYIADN HVYFDVLSAP NYTELSNRNL EAMFEGVRIE NSKTKKNPQD FVLWKPAKKN EPENINFTSP WGLGRPGWHI ECSAMSYKYL GQNFDIHGGG ADLIFPHHTN EIAQSRCAFP SSTYAKYWIH NGFLTVNGSK MSKSLGNFIT VRDLMDKQIQ GEVVRLFLLS SHYRRPLDYN DKAIDDAKKT LDYWYRAIEH INVQKVDLPS NFMRSLFDDM NTPLAIKIIN DYAKCVFISK TEAERKFNAS AIITCANFIG LMRKTQYEWF NRDVDEFYIN ELINKRLKAK KQKNWLLADK IRNQLLEKKI ILEDKSNYIT IWRKE

Validated NT Sequence

atggctcacc accnccacca ccatatgaaa cttcacttat ataacactct gactcgtact aaagaggttt ttaatcctca agatcctgct aatataaaaa tgtatgtgtg tggaccgact gtttatgata atcctcatat aggtaatagt aggtcagtag tagtatatga tttactttat cgtataatta taaaaatttt cggtaaaaag gccgtaaaat atgtacgtaa catcactgat gttgatgata aaattattga tagagctgaa ttactcggta ttactattaa tgatttgact gataaagtta cgcgagagtt tcatataaat atggaatatt taggttgcat gttaccaact attgagccta aagctacaga gcatattgat gtaatgatcg aaataataga acgcttaata gcaaaagatc atgcatatat tgctgataat catgtttatt ttgatgtttt atcagctcca aattatacag aattatctaa tcgcaattta gaagcaatgt ttgagggagt tcgtatagaa aatagtaaaa ctaaaaaaaa cccacaggac tttgtattat ggaagccagc aaagaaaaat gagccggaaa atataaattt tacaagtcct tgggggcttg gtcggcctgg atggcatatt gaatgttcag ccatgagcta taaatattta gggcaaaatt ttgatattca tggaggtgga gcagatttaa ttttcccaca tcataccaat gagattgcac aaagtagatg tgcttttcca agttctacct atgccaaata ttggatacat aatggttttt taactgtaaa cggtagcaaa atgagcaaat ctcttggtaa ttttataaca gtaagggatt taatggataa gcaaattcaa ggagaagtag tacgactatt cttattaagt agtcactata ggcgcccact cgattataat gataaagcta tagatgatgc taagaaaact ttagattatt ggtatcgagc tatagaacac attaatgtac aaaaagtaga tttaccgtct aatttcatgc gaagcttatt tgatgatatg aatactcccc ttgccataaa aataatcaat gactacgcta aatgtgtttt tatttctaaa actgaggcag aaagaaagtt taatgcttct gctattatta cttgcgctaa ttttattggt ttaatgcgta agactcaata tgagtggttt aatcgtgatg ttgatgaatt ttatataaat gaacttataa ataaacgttt gaaagcaaaa aaacaaaaaa attggctgtt agctgataaa attcgtaatc agctattaga gaaaaaaata attctagaag ataaatctaa ctatattact atttggcgca aagaataagt gagtaagat

Expected Protein Sequence

MAHHHHHHMK LHLYNTLTRT KEVFNPQDPA NIKMYVCGPT VYDNPHIGNS RSVVVYDLLY RIIIKIFGKK AVKYVRNITD VDDKIIDRAE LLGITINDLT DKVTREFHIN MEYLGCMLPT IEPKATEHID VMIEIIERLI AKDHAYIADN HVYFDVLSAP NYTELSNRNL EAMFEGVRIE NSKTKKNPQD FVLWKPAKKN EPENINFTSP WGLGRPGWHI ECSAMSYKYL GQNFDIHGGG ADLIFPHHTN EIAQSRCAFP SSTYAKYWIH NGFLTVNGSK MSKSLGNFIT VRDLMDKQIQ GEVVRLFLLS SHYRRPLDYN DKAIDDAKKT LDYWYRAIEH INVQKVDLPS NFMRSLFDDM NTPLAIKIIN DYAKCVFISK TEAERKFNAS AIITCANFIG LMRKTQYEWF NRDVDEFYIN ELINKRLKAK KQKNWLLADK IRNQLLEKKI ILEDKSNYIT IWRKE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac ttcacttata taacactctg actcgtacta aagaggtttt taatcctcaa gatcctgcta atataaaaat gtatgtgtgt ggaccgactg tttatgataa tcctcatata ggtaatagta ggtcagtagt agtatatgat ttactttatc gtataattat aaaaattttc ggtaaaaagg ccgtaaaata tgtacgtaac atcactgatg ttgatgataa aattattgat agagctgaat tactcggtat tactattaat gatttgactg ataaagttac gcgagagttt catataaata tggaatattt aggttgcatg ttaccaacta ttgagcctaa agctacagag catattgatg taatgatcga aataatagaa cgcttaatag caaaagatca tgcatatatt gctgataatc atgtttattt tgatgtttta tcagctccaa attatacaga attatctaat cgcaatttag aagcaatgtt tgagggagtt cgtatagaaa atagtaaaac taaaaaaaac ccacaggact ttgtattatg gaagccagca aagaaaaatg agccggaaaa tataaatttt acaagtcctt gggggcttgg tcggcctgga tggcatattg aatgttcagc catgagctat aaatatttag ggcaaaattt tgatattcat ggaggtggag cagatttaat tttcccacat cataccaatg agattgcaca aagtagatgt gcttttccaa gttctaccta tgccaaatat tggatacata atggtttttt aactgtgaac ggtagcaaaa tgagcaaatc tcttggtaat tttataacag taagggattt aatggataag caaattcaag gagaagtagt acgactattc ttattaagta gtcactatag gcgcccactc gattataatg ataaagctat agatgatgct aagaaaactt tagattattg gtatcgagct atagaacaca ttaatgtaca aaaagtagat ttaccgtcta atttcatgcg aagcttattt gatgatatga atactcccct tgccataaaa ataatcaatg actacgctaa atgtgttttt atttctaaaa ctgaggcaga aagaaagttt aatgcttctg ctattattac ttgcgctaat tttattggtt taatgcgtaa gactcaatat gagtggttta atcgtgatgt tgatgaattt tatataaatg aacttataaa taaacgtttg aaagcaaaaa aacaaaaaaa ttggctgtta gctgataaaa ttcgtaatca gctattagag aaaaaaataa ttctagaaga taaatctaac tatattacta tttggcgcaa agaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat