RiprA.00119.a

D-alanine--D-alanine ligase

CENTER ID: RiprA.00119.a
ORGANISM: Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE: Endemic typhus
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiprA.00119.a.A1.GE32593 Full length( RiprA.00119.a ) 1 321
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiprA.00119.a.A1.PW32513 Full length( RiprA.00119.a ) 1 321

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272947.5.peg.256
RefSeq: NP_220634.1
UniProt: Q9ZDS6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHKYQTHFVE HSVVKILSST GKKHIALVAG GMSAEREVSL VSSAGVSKAL IELGYKVTFV DMGADIAVKL QEINPDIVFN CLHGTYGEDG CLPGLLNIMR IPYTHSGLLS SALAFDKIHF RSCVLKNNIN IADSIVVSKS DHINTDPMKR PYVIKPLKQG SSIGVEVIFE EDDFNFTDYD FPYGEDIIIE QYIQGQELQV ALLNGKALGV LEIKLLKNRF YDYETKYNEG FAEHVCPAPL PANLYKKLLI ESEKIYKTIN CKGPVRAEFI LEEQTNKLYV LEINTHPGMT PLSIVPEIAA YAGINFTNLI EEIIKMASFE S
NT Sequence
ATGCACAAAT ATCAAACACA TTTTGTAGAA CACTCTGTAG TCAAAATATT AAGTAGTACA GGTAAAAAGC ATATAGCATT AGTAGCGGGT GGTATGTCTG CTGAGCGTGA AGTCTCTTTA GTATCCAGTG CAGGAGTTAG TAAAGCTTTA ATTGAATTAG GATATAAAGT TACATTTGTT GATATGGGTG CAGATATTGC AGTAAAATTA CAAGAAATAA ACCCTGATAT TGTTTTTAAC TGTTTACATG GTACATATGG TGAAGATGGT TGTTTACCAG GATTACTTAA TATAATGCGT ATTCCTTATA CTCATAGTGG TCTATTATCT TCGGCTTTAG CATTCGATAA AATACACTTT AGAAGCTGTG TCTTAAAAAA TAATATTAAC ATTGCAGACA GTATTGTCGT TAGTAAAAGT GATCATATAA ACACTGATCC TATGAAGCGA CCGTATGTAA TAAAACCTCT TAAACAAGGA TCAAGTATTG GTGTTGAAGT AATATTTGAA GAGGATGATT TTAACTTTAC CGATTATGAT TTTCCTTATG GTGAAGATAT AATAATAGAG CAGTATATAC AAGGGCAAGA ATTGCAAGTA GCATTACTAA ATGGCAAAGC TTTAGGTGTT TTAGAAATTA AATTACTAAA AAATCGTTTT TATGATTATG AAACTAAATA TAATGAAGGA TTTGCTGAAC ATGTGTGTCC TGCTCCGCTA CCTGCTAATT TATATAAAAA ATTACTGATA GAATCAGAAA AAATTTATAA AACAATAAAT TGTAAAGGTC CTGTTAGAGC AGAGTTCATT TTAGAAGAAC AGACAAACAA ATTATATGTT TTGGAGATCA ATACTCACCC TGGGATGACG CCTTTATCAA TAGTGCCAGA AATTGCAGCC TATGCAGGGA TAAATTTTAC TAATTTAATT GAAGAAATTA TAAAGATGGC AAGCTTTGAA TCA
Details for RiprA.00119.a.A1.GE32593
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMHKYQTH FVEHSVVKIL SSTGKKHIAL VAGGMSAERE VSLVSSAGVS KALIELGYKV TFVDMGADIA VKLQEINPDI VFNCLHGTYG EDGCLPGLLN IMRIPYTHSG LLSSALAFDK IHFRSCVLKN NINIADSIVV SKSDHINTDP MKRPYVIKPL KQGSSIGVEV IFEEDDFNFT DYDFPYGEDI IIEQYIQGQE LQVALLNGKA LGVLEIKLLK NRFYDYETKY NEGFAEHVCP APLPANLYKK LLIESEKIYK TINCKGPVRA EFILEEQTNK LYVLEINTHP GMTPLSIVPE IAAYAGINFT NLIEEIIKMA SF
Validated NT Sequence
catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcacaaa tatcaaacac attttgtaga acactctgta gtcaaaatat taagtagtac aggtaaaaag catatagcat tagtagcggg tggtatgtct gctgagcgtg aagtctcttt agtatccagt gcaggagtta gtaaagcttt aattgaatta ggatataaag ttacatttgt tgatatgggt gcagatattg cagtaaaatt acaagaaata aaccctgata ttgtttttaa ctgtttacat ggtacatatg gtgaagatgg ttgtttacca ggattactta atataatgcg tattccttat actcatagtg gtctattatc ttcggcttta gcattcgata aaatacactt tagaagctgt gtcttaaaaa ataatattaa cattgcagac agtattgtcg ttagtaaaag tgatcatata aacactgatc ctatgaagcg accgtatgta ataaaacctc ttaaacaagg atcaagtatt ggtgttgaag taatatttga agaggatgat tttaacttta ccgattatga ttttccttat ggtgaagata taataataga gcagtatata caagggcaag aattgcaagt agcattacta aatggcaaag ctttaggtgt tttagaaatt aaattactaa aaaatcgttt ttatgattat gaaactaaat ataatgaagg atttgctgaa catgtgtgtc ctgctccgct acctgctaat ttatataaaa aattactgat agaatcagaa aaaatttata aaacaataaa ttgtaaaggt cctgttagag cagagttcat tttagaagaa cagacaaaca aattatatgt tttggagatc aatactcacc ctgggatgac gcctttatca atagtgccag aaattgcagc ctatgcaggg ataaatttta ctaatttaat tgaagaaatt ataaagatgg caagctttg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHKYQTHFV EHSVVKILSS TGKKHIALVA GGMSAEREVS LVSSAGVSKA LIELGYKVTF VDMGADIAVK LQEINPDIVF NCLHGTYGED GCLPGLLNIM RIPYTHSGLL SSALAFDKIH FRSCVLKNNI NIADSIVVSK SDHINTDPMK RPYVIKPLKQ GSSIGVEVIF EEDDFNFTDY DFPYGEDIII EQYIQGQELQ VALLNGKALG VLEIKLLKNR FYDYETKYNE GFAEHVCPAP LPANLYKKLL IESEKIYKTI NCKGPVRAEF ILEEQTNKLY VLEINTHPGM TPLSIVPEIA AYAGINFTNL IEEIIKMASF ES
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcacaaatat caaacacatt ttgtagaaca ctctgtagtc aaaatattaa gtagtacagg taaaaagcat atagcattag tagcgggtgg tatgtctgct gagcgtgaag tctctttagt atccagtgca ggagttagta aagctttaat tgaattagga tataaagtta catttgttga tatgggtgca gatattgcag taaaattaca agaaataaac cctgatattg tttttaactg tttacatggt acatatggtg aagatggttg tttaccagga ttacttaata taatgcgtat tccttatact catagtggtc tattatcttc ggctttagca ttcgataaaa tacactttag aagctgtgtc ttaaaaaata atattaacat tgcagacagt attgtcgtta gtaaaagtga tcatataaac actgatccta tgaagcgacc gtatgtaata aaacctctta aacaaggatc aagtattggt gttgaagtaa tatttgaaga ggatgatttt aactttaccg attatgattt tccttatggt gaagatataa taatagagca gtatatacaa gggcaagaat tgcaagtagc attactaaat ggcaaagctt taggtgtttt agaaattaaa ttactaaaaa atcgttttta tgattatgaa actaaatata atgaaggatt tgctgaacat gtgtgtcctg ctccgctacc tgctaattta tataaaaaat tactgataga atcagaaaaa atttataaaa caataaattg taaaggtcct gttagagcag agttcatttt agaagaacag acaaacaaat tatatgtttt ggagatcaat actcaccctg ggatgacgcc tttatcaata gtgccagaaa ttgcagccta tgcagggata aattttacta atttaattga agaaattata aagatggcaa gctttgaatc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for RiprA.00119.a.A1.PW32513
PURIFICATION DATe: 7/29/2011
CONCENTRATION: 28.31mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMHKYQTH FVEHSVVKIL SSTGKKHIAL VAGGMSAERE VSLVSSAGVS KALIELGYKV TFVDMGADIA VKLQEINPDI VFNCLHGTYG EDGCLPGLLN IMRIPYTHSG LLSSALAFDK IHFRSCVLKN NINIADSIVV SKSDHINTDP MKRPYVIKPL KQGSSIGVEV IFEEDDFNFT DYDFPYGEDI IIEQYIQGQE LQVALLNGKA LGVLEIKLLK NRFYDYETKY NEGFAEHVCP APLPANLYKK LLIESEKIYK TINCKGPVRA EFILEEQTNK LYVLEINTHP GMTPLSIVPE IAAYAGINFT NLIEEIIKMA SF
Validated NT Sequence
catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcacaaa tatcaaacac attttgtaga acactctgta gtcaaaatat taagtagtac aggtaaaaag catatagcat tagtagcggg tggtatgtct gctgagcgtg aagtctcttt agtatccagt gcaggagtta gtaaagcttt aattgaatta ggatataaag ttacatttgt tgatatgggt gcagatattg cagtaaaatt acaagaaata aaccctgata ttgtttttaa ctgtttacat ggtacatatg gtgaagatgg ttgtttacca ggattactta atataatgcg tattccttat actcatagtg gtctattatc ttcggcttta gcattcgata aaatacactt tagaagctgt gtcttaaaaa ataatattaa cattgcagac agtattgtcg ttagtaaaag tgatcatata aacactgatc ctatgaagcg accgtatgta ataaaacctc ttaaacaagg atcaagtatt ggtgttgaag taatatttga agaggatgat tttaacttta ccgattatga ttttccttat ggtgaagata taataataga gcagtatata caagggcaag aattgcaagt agcattacta aatggcaaag ctttaggtgt tttagaaatt aaattactaa aaaatcgttt ttatgattat gaaactaaat ataatgaagg atttgctgaa catgtgtgtc ctgctccgct acctgctaat ttatataaaa aattactgat agaatcagaa aaaatttata aaacaataaa ttgtaaaggt cctgttagag cagagttcat tttagaagaa cagacaaaca aattatatgt tttggagatc aatactcacc ctgggatgac gcctttatca atagtgccag aaattgcagc ctatgcaggg ataaatttta ctaatttaat tgaagaaatt ataaagatgg caagctttg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHKYQTHFV EHSVVKILSS TGKKHIALVA GGMSAEREVS LVSSAGVSKA LIELGYKVTF VDMGADIAVK LQEINPDIVF NCLHGTYGED GCLPGLLNIM RIPYTHSGLL SSALAFDKIH FRSCVLKNNI NIADSIVVSK SDHINTDPMK RPYVIKPLKQ GSSIGVEVIF EEDDFNFTDY DFPYGEDIII EQYIQGQELQ VALLNGKALG VLEIKLLKNR FYDYETKYNE GFAEHVCPAP LPANLYKKLL IESEKIYKTI NCKGPVRAEF ILEEQTNKLY VLEINTHPGM TPLSIVPEIA AYAGINFTNL IEEIIKMASF ES
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCACAAATAT CAAACACATT TTGTAGAACA CTCTGTAGTC AAAATATTAA GTAGTACAGG TAAAAAGCAT ATAGCATTAG TAGCGGGTGG TATGTCTGCT GAGCGTGAAG TCTCTTTAGT ATCCAGTGCA GGAGTTAGTA AAGCTTTAAT TGAATTAGGA TATAAAGTTA CATTTGTTGA TATGGGTGCA GATATTGCAG TAAAATTACA AGAAATAAAC CCTGATATTG TTTTTAACTG TTTACATGGT ACATATGGTG AAGATGGTTG TTTACCAGGA TTACTTAATA TAATGCGTAT TCCTTATACT CATAGTGGTC TATTATCTTC GGCTTTAGCA TTCGATAAAA TACACTTTAG AAGCTGTGTC TTAAAAAATA ATATTAACAT TGCAGACAGT ATTGTCGTTA GTAAAAGTGA TCATATAAAC ACTGATCCTA TGAAGCGACC GTATGTAATA AAACCTCTTA AACAAGGATC AAGTATTGGT GTTGAAGTAA TATTTGAAGA GGATGATTTT AACTTTACCG ATTATGATTT TCCTTATGGT GAAGATATAA TAATAGAGCA GTATATACAA GGGCAAGAAT TGCAAGTAGC ATTACTAAAT GGCAAAGCTT TAGGTGTTTT AGAAATTAAA TTACTAAAAA ATCGTTTTTA TGATTATGAA ACTAAATATA ATGAAGGATT TGCTGAACAT GTGTGTCCTG CTCCGCTACC TGCTAATTTA TATAAAAAAT TACTGATAGA ATCAGAAAAA ATTTATAAAA CAATAAATTG TAAAGGTCCT GTTAGAGCAG AGTTCATTTT AGAAGAACAG ACAAACAAAT TATATGTTTT GGAGATCAAT ACTCACCCTG GGATGACGCC TTTATCAATA GTGCCAGAAA TTGCAGCCTA TGCAGGGATA AATTTTACTA ATTTAATTGA AGAAATTATA AAGATGGCAA GCTTTGAATC ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA