SSGCID: Target Page

RiprA.00081.b

NIFS PROTEIN HOMOLOG (Spl1)

CENTER ID:	RiprA.00081.b
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00081.b.B1.GE35310	Full length( RiprA.00081.b )		1	376

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00081.b.B1.PW37229	Full length( RiprA.00081.b )		1	376

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272947.5.peg.497
RefSeq:	NP_220864.1
UniProt:	Q9ZD59

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIYLDHNATT FIDPRVKEYI ISLMDKELNP SSAHTSGRFA KNIIETARSQ IATSLGITMS REYNITFTSS GTESNNLIMK NFYDGDIFIS AIEHLSIYNH INYAPNIKII RVNKQGLVDL EHLEDLLSQS NASKKLVSVM IANNENGVLQ DIAAISKITK KYNAKFHSDL VQSFGKISLN IKELGLDFAT ISGHKIGGGH GCGALISNSN FQLIPIIIGG GQEKSVRSGT ENVLAIAGFG LAAEFRRKDI SKNYIKIRYL QERLEQKLKE YSNVNIISNN VARLANTTLI TVDNTDAQVK LIGFDLRNIC VSSGSACSSG KISKSHVLTN MGIREEEARS SIRISLSHTN TISDIKAFIK AFEEIYEYKS SCNHCI

NT Sequence

ATGATATATC TTGATCATAA TGCTACTACT TTCATTGATC CTAGAGTTAA GGAATATATA ATAAGTTTAA TGGATAAGGA GCTTAACCCT TCATCCGCAC ATACTTCTGG AAGATTTGCT AAGAACATTA TAGAAACTGC ACGTTCACAA ATCGCAACAT CTCTTGGTAT AACAATGTCT AGGGAATATA ATATTACTTT TACATCTTCT GGAACTGAAA GTAATAATTT AATAATGAAA AATTTTTATG ATGGTGATAT TTTTATTTCA GCTATTGAAC ATTTATCGAT TTATAACCAT ATAAATTATG CGCCAAATAT TAAAATTATA AGAGTTAATA AGCAAGGACT AGTGGATTTA GAACATCTAG AAGACTTATT ATCTCAAAGT AATGCTAGTA AAAAACTAGT TTCCGTAATG ATTGCAAATA ATGAAAATGG AGTGTTACAG GATATAGCTG CGATAAGTAA AATAACTAAA AAATATAATG CAAAATTTCA TAGTGATTTA GTACAAAGTT TTGGCAAGAT ATCTTTAAAT ATTAAAGAAT TAGGGTTAGA TTTTGCTACG ATTTCAGGAC ATAAAATAGG AGGAGGGCAT GGTTGTGGTG CTTTAATCTC TAACTCTAAT TTTCAGCTTA TCCCAATAAT TATAGGAGGA GGTCAGGAAA AATCAGTGCG CTCAGGCACT GAAAATGTTT TAGCAATTGC TGGCTTTGGT TTAGCTGCTG AATTTAGAAG AAAAGATATC TCAAAAAATT ACATAAAAAT CAGGTACTTA CAAGAAAGAT TAGAGCAAAA ACTAAAAGAA TATTCAAACG TAAATATTAT TAGTAATAAT GTAGCTAGGT TAGCTAATAC TACCTTAATT ACTGTAGACA ATACTGATGC ACAAGTAAAA TTAATAGGGT TTGATTTACG TAATATTTGT GTAAGCTCTG GGTCTGCTTG CTCATCAGGT AAAATATCTA AATCGCATGT ATTAACGAAT ATGGGAATAA GAGAAGAAGA GGCTAGGTCT TCAATTCGTA TATCTTTAAG TCATACTAAT ACGATAAGTG ATATAAAAGC TTTTATAAAA GCTTTTGAGG AGATATATGA ATATAAATCG TCTTGTAATC ACTGTATA

Details for RiprA.00081.b.B1.GE35310

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	87

Validated AA Sequence

MDKELNPSSA HTSGRFAKNI IETARSQIAT SLGITMSREY NITFTSSGTE SNNLIMKNFY DGDIFISAIE HLSIYNHINY APNIKIIRVN KQGLVDLEHL EDLLSQSNAS KKLVSVMIAN NENGVLQDIA AISKITKKYN AKFHSDLVQS FGKISLNIKE LGLDFATISG HKIGGGHGCG ALISNSNFQL IPIIIGGGQE KSVRSGTENV LAIAGFGLAA EFRRKDISKN YIKIRYLQER LEQKLKEYSN VNIISNNVAR LANTTLITVD NTDAQVKLIG FDLRNICVSS GSACSSGKIS KSHVLTNMGI REEEARSSIR ISLSHTNTIS DIKAFIK

Validated NT Sequence

gcttttataa aagcttttat atcacttatc gtattagtat gacttaaaga tatacgaatt gaagacctag cctcttcttc tcttattccc atattcgtta atacatgcga tttagatatt ttacctgatg agcaagcaga cccagagctt acacaaatat tacgtaaatc aaaccctatt aattttactt gtgcatcagt attgtctaca gtaattaagg tagtattagc taacctagct acattattac taataatatt tacgtttgaa tattctttta gtttttgctc taatctttct tgtaagtacc tgatttttat gtaatttttt gagatatctt ttcttctaaa ttcagcagct aaaccaaagc cagcaattgc taaaacattt tcagtgcctg agcgcactga tttttcctga cctcctccta taattattgg gataagctga aaattagagt tagagattaa agcaccacaa ccatgccctc ctcctatttt atgtcctgaa atcgtagcaa aatctaaccc taattcttta atatttaaag atatcttgcc aaaactttgt actaaatcac tatgaaattt tgcattatat tttttagtta ttttacttat cgcagctata tcctgtaaca ctccattttc attatttgca atcattacgg aaactagttt tttactagca ttactttgag ataataagtc ttctagatgt tctaaatcca ctagtccttg cttattaact cttataattt taatatttgg cgcataattt atatggttat aaatcgataa atgttcaata gctgaaataa aaatatcacc atcataaaaa tttttcatta ttaaattatt actttcagtt ccagaagatg taaaagtaat attatattcc ctagacattg ttataccaag agatgttgcg atttgtgaac gtgcagtttc tataatgttc ttagcaaatc ttccagaagt atgtgcggat gaagggttaa gctccttatc cattaaactt attatatatt ccttaactct aggatcaatg aaagtagtag cattatgatc aagat

Expected Protein Sequence

MAHHHHHHMI YLDHNATTFI DPRVKEYIIS LMDKELNPSS AHTSGRFAKN IIETARSQIA TSLGITMSRE YNITFTSSGT ESNNLIMKNF YDGDIFISAI EHLSIYNHIN YAPNIKIIRV NKQGLVDLEH LEDLLSQSNA SKKLVSVMIA NNENGVLQDI AAISKITKKY NAKFHSDLVQ SFGKISLNIK ELGLDFATIS GHKIGGGHGC GALISNSNFQ LIPIIIGGGQ EKSVRSGTEN VLAIAGFGLA AEFRRKDISK NYIKIRYLQE RLEQKLKEYS NVNIISNNVA RLANTTLITV DNTDAQVKLI GFDLRNICVS SGSACSSGKI SKSHVLTNMG IREEEARSSI RISLSHTNTI SDIKAFIKAF EEIYEYKSSC NHCI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatat atcttgatca taatgctact actttcattg atcctagagt taaggaatat ataataagtt taatggataa ggagcttaac ccttcatccg cacatacttc tggaagattt gctaagaaca ttatagaaac tgcacgttca caaatcgcaa catctcttgg tataacaatg tctagggaat ataatattac ttttacatct tctggaactg aaagtaataa tttaataatg aaaaattttt atgatggtga tatttttatt tcagctattg aacatttatc gatttataac catataaatt atgcgccaaa tattaaaatt ataagagtta ataagcaagg actagtggat ttagaacatc tagaagactt attatctcaa agtaatgcta gtaaaaaact agtttccgta atgattgcaa ataatgaaaa tggagtgtta caggatatag ctgcgataag taaaataact aaaaaatata atgcaaaatt tcatagtgat ttagtacaaa gttttggcaa gatatcttta aatattaaag aattagggtt agattttgct acgatttcag gacataaaat aggaggaggg catggttgtg gtgctttaat ctctaactct aattttcagc ttatcccaat aattatagga ggaggtcagg aaaaatcagt gcgctcaggc actgaaaatg ttttagcaat tgctggcttt ggtttagctg ctgaatttag aagaaaagat atctcaaaaa attacataaa aatcaggtac ttacaagaaa gattagagca aaaactaaaa gaatattcaa acgtaaatat tattagtaat aatgtagcta ggttagctaa tactacctta attactgtag acaatactga tgcacaagta aaattaatag ggtttgattt acgtaatatt tgtgtaagct ctgggtctgc ttgctcatca ggtaaaatat ctaaatcgca tgtattaacg aatatgggaa taagagaaga agaggctagg tcttcaattc gtatatcttt aagtcatact aatacgataa gtgatataaa agcttttata aaagcttttg aggagatata tgaatataaa tcgtcttgta atcactgtat atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for RiprA.00081.b.B1.PW37229

PURIFICATION DATe:	12/13/2012
CONCENTRATION:	28.1mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	87

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATAT ATCTTGATCA TAATGCTACT ACTTTCATTG ATCCTAGAGT TAAGGAATAT ATAATAAGTT TAATGGATAA GGAGCTTAAC CCTTCATCCG CACATACTTC TGGAAGATTT GCTAAGAACA TTATAGAAAC TGCACGTTCA CAAATCGCAA CATCTCTTGG TATAACAATG TCTAGGGAAT ATAATATTAC TTTTACATCT TCTGGAACTG AAAGTAATAA TTTAATAATG AAAAATTTTT ATGATGGTGA TATTTTTATT TCAGCTATTG AACATTTATC GATTTATAAC CATATAAATT ATGCGCCAAA TATTAAAATT ATAAGAGTTA ATAAGCAAGG ACTAGTGGAT TTAGAACATC TAGAAGACTT ATTATCTCAA AGTAATGCTA GTAAAAAACT AGTTTCCGTA ATGATTGCAA ATAATGAAAA TGGAGTGTTA CAGGATATAG CTGCGATAAG TAAAATAACT AAAAAATATA ATGCAAAATT TCATAGTGAT TTAGTACAAA GTTTTGGCAA GATATCTTTA AATATTAAAG AATTAGGGTT AGATTTTGCT ACGATTTCAG GACATAAAAT AGGAGGAGGG CATGGTTGTG GTGCTTTAAT CTCTAACTCT AATTTTCAGC TTATCCCAAT AATTATAGGA GGAGGTCAGG AAAAATCAGT GCGCTCAGGC ACTGAAAATG TTTTAGCAAT TGCTGGCTTT GGTTTAGCTG CTGAATTTAG AAGAAAAGAT ATCTCAAAAA ATTACATAAA AATCAGGTAC TTACAAGAAA GATTAGAGCA AAAACTAAAA GAATATTCAA ACGTAAATAT TATTAGTAAT AATGTAGCTA GGTTAGCTAA TACTACCTTA ATTACTGTAG ACAATACTGA TGCACAAGTA AAATTAATAG GGTTTGATTT ACGTAATATT TGTGTAAGCT CTGGGTCTGC TTGCTCATCA GGTAAAATAT CTAAATCGCA TGTATTAACG AATATGGGAA TAAGAGAAGA AGAGGCTAGG TCTTCAATTC GTATATCTTT AAGTCATACT AATACGATAA GTGATATAAA AGCTTTTATA AAAGCTTTTG AGGAGATATA TGAATATAAA TCGTCTTGTA ATCACTGTAT ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT