RiprA.00075.a

delta-aminolevulinic acid dehydratase

CENTER ID:	RiprA.00075.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00075.a.A1.GE25415	Full length( RiprA.00075.a )		1	330

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00075.a.A1.PS00064	Full length( RiprA.00075.a )		1	330

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127486
PATRIC ID:	fig\|272947.5.peg.550
RefSeq:	NP_220912
UniProt:	Q9ZD11

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYPLIRFRRN RKALWLRELI AESNLSISDL VLPLFVVEGH NEMQEIKTMP GIYRLSIDQI VETAKKAADF GINAIALFPS IDHSLKSDNA DEAYNLDNLI CRTIIKIKNA NIDIGIICDV ALDPYTISGH DGIVHHGEVD NDRSVKALCN QALVLAKAGV DIVAPSDMMD GRIAAIREYL DKEGFINVGI LAYAAKYASS FYGPFRDAVQ SNKKNYLDKS SYQIDVRNIK EAMLEIEHDI AEGADIVMVK PGMPFLDVIR EAANNFNAKI FAYQVSGEYA MLKFAAETGA IDWERALIES LISFKRAGAT GIFTYAALEV AEILKHNNIK

NT Sequence

ATGTACCCTC TTATTAGATT TAGAAGAAAT AGAAAAGCTT TATGGCTTAG AGAGTTAATA GCAGAGAGTA ATTTATCGAT TAGTGATTTA GTGTTACCTT TATTTGTTGT TGAAGGCCAT AATGAAATGC AAGAAATTAA GACTATGCCT GGTATATATC GTTTATCGAT CGATCAGATA GTTGAAACTG CAAAAAAAGC AGCAGATTTT GGTATTAATG CGATTGCATT ATTTCCTAGT ATTGATCACA GTCTAAAAAG TGACAATGCA GATGAGGCAT ATAATTTAGA TAATTTAATA TGTAGAACTA TTATAAAAAT CAAAAACGCC AATATTGACA TTGGTATAAT ATGTGATGTA GCACTTGATC CTTACACTAT AAGTGGCCAT GATGGTATTG TTCATCATGG AGAAGTTGAT AATGATAGGT CAGTAAAAGC TTTATGTAAT CAAGCATTAG TATTAGCAAA AGCTGGAGTA GATATCGTTG CACCTTCTGA TATGATGGAT GGAAGAATAG CAGCTATCAG GGAGTATCTT GACAAAGAAG GCTTTATAAA TGTAGGAATT CTTGCTTATG CAGCTAAATA TGCTTCAAGC TTTTATGGCC CATTTCGCGA TGCAGTTCAG AGTAATAAAA AGAATTATTT AGATAAATCA AGCTATCAAA TTGATGTGCG TAATATTAAG GAAGCAATGC TTGAGATTGA GCATGATATA GCAGAAGGAG CAGATATTGT AATGGTTAAA CCAGGTATGC CGTTTTTGGA TGTGATTCGT GAAGCGGCAA ATAATTTTAA TGCTAAAATT TTTGCATATC AGGTTAGTGG GGAATACGCA ATGTTAAAAT TTGCAGCAGA AACAGGGGCT ATTGATTGGG AGAGAGCGTT AATAGAATCA TTAATTAGTT TTAAACGTGC CGGTGCAACA GGAATATTTA CCTATGCTGC TCTTGAAGTA GCTGAAATAT TAAAGCACAA TAACATTAAG

Details for RiprA.00075.a.A1.GE25415

HARVESTED ON:	6/30/2008
SEQUENCED ON:	3/6/2009
EXPECTED MW:	38kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMYPLIRFRR NRKALWLREL IAESNLSISD LVLPLFVVEG HNEMQEIKTM PGIYRLSIDQ IVETAKKAAD FGINAIALFP SIDHSLKSDN ADEAYNLDNL ICRTIIKIKN ANIDIGIICD VALDPYTISG HDGIVHHGEV DNDRSVKALC NQALVLAKAG VDIVAPSDMM DGRIAAIREY LDKEGFINVG ILAYAAKYAS SFYGPFRDAV QSNKKNYLDK SSYQIDVRNI KEAMLEIEHD IAEGADIVMV KPGMPFLDVI REAANNFNAK IFAYQVSGEY AMLKFAAETG AIDWERALIE SLISFKRAGA TGIFTYAALE VAEILKHNNI K

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtacc ctcttattag atttagaaga aatagaaaag ctttatggct tagagagtta atagcagaga gtaatttatc gattagtgat ttagtgttac ctttatttgt tgttgaaggc cataatgaaa tgcaagaaat taagactatg cctggtatat atcgtttatc gatcgatcag atagttgaaa ctgcaaaaaa agcagcagat tttggtatta atgcgattgc attatttcct agtattgatc acagtctaaa aagtgacaat gcagatgagg catataattt agataattta atatgtagaa ctattataaa aatcaaaaac gccaatattg acattggtat aatatgtgat gtagcacttg atccttacac tataagtggc catgatggta ttgttcatca tggagaagtt gataatgata ggtcagtaaa agctttatgt aatcaagcat tagtattagc aaaagctgga gtagatatcg ttgcaccttc tgatatgatg gatggaagaa tagcagctat cagggagtat cttgacaaag aaggctttat aaatgtagga attcttgctt atgcagctaa atatgcttca agcttttatg gcccatttcg cgatgcagtt cagagtaata aaaagaatta tttagataaa tcaagctatc aaattgatgt gcgtaatatt aaggaagcaa tgcttgagat tgagcatgat atagcagaag gagcagatat tgtaatggtt aaaccaggta tgccgttttt ggatgtgatt cgtgaagcgg caaataattt taatgctaaa atttttgcat atcaggttag tggggaatac gcaatgttaa aatttgcagc agaaacaggg gctattgatt gggagagagc gttaatagaa tcattaatta gttttaaacg tgccggtgca acaggaatat ttacctatgc tgctcttgaa gtagctgaaa tattaaagca caataacatt aagtaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtaccctctt attagattta gaagaaatag aaaagcttta tggcttagag agttaatagc agagagtaat ttatcgatta gtgatttagt gttaccttta tttgttgttg aaggccataa tgaaatgcaa gaaattaaga ctatgcctgg tatatatcgt ttatcgatcg atcagatagt tgaaactgca aaaaaagcag cagattttgg tattaatgcg attgcattat ttcctagtat tgatcacagt ctaaaaagtg acaatgcaga tgaggcatat aatttagata atttaatatg tagaactatt ataaaaatca aaaacgccaa tattgacatt ggtataatat gtgatgtagc acttgatcct tacactataa gtggccatga tggtattgtt catcatggag aagttgataa tgataggtca gtaaaagctt tatgtaatca agcattagta ttagcaaaag ctggagtaga tatcgttgca ccttctgata tgatggatgg aagaatagca gctatcaggg agtatcttga caaagaaggc tttataaatg taggaattct tgcttatgca gctaaatatg cttcaagctt ttatggccca tttcgcgatg cagttcagag taataaaaag aattatttag ataaatcaag ctatcaaatt gatgtgcgta atattaagga agcaatgctt gagattgagc atgatatagc agaaggagca gatattgtaa tggttaaacc aggtatgccg tttttggatg tgattcgtga agcggcaaat aattttaatg ctaaaatttt tgcatatcag gttagtgggg aatacgcaat gttaaaattt gcagcagaaa caggggctat tgattgggag agagcgttaa tagaatcatt aattagtttt aaacgtgccg gtgcaacagg aatatttacc tatgctgctc ttgaagtagc tgaaatatta aagcacaata acattaagta gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for RiprA.00075.a.A1.PS00064

PURIFICATION DATe:	8/25/2008
CONCENTRATION:	46.06mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTACCCTCTT ATTAGATTTA GAAGAAATAG AAAAGCTTTA TGGCTTAGAG AGTTAATAGC AGAGAGTAAT TTATCGATTA GTGATTTAGT GTTACCTTTA TTTGTTGTTG AAGGCCATAA TGAAATGCAA GAAATTAAGA CTATGCCTGG TATATATCGT TTATCGATCG ATCAGATAGT TGAAACTGCA AAAAAAGCAG CAGATTTTGG TATTAATGCG ATTGCATTAT TTCCTAGTAT TGATCACAGT CTAAAAAGTG ACAATGCAGA TGAGGCATAT AATTTAGATA ATTTAATATG TAGAACTATT ATAAAAATCA AAAACGCCAA TATTGACATT GGTATAATAT GTGATGTAGC ACTTGATCCT TACACTATAA GTGGCCATGA TGGTATTGTT CATCATGGAG AAGTTGATAA TGATAGGTCA GTAAAAGCTT TATGTAATCA AGCATTAGTA TTAGCAAAAG CTGGAGTAGA TATCGTTGCA CCTTCTGATA TGATGGATGG AAGAATAGCA GCTATCAGGG AGTATCTTGA CAAAGAAGGC TTTATAAATG TAGGAATTCT TGCTTATGCA GCTAAATATG CTTCAAGCTT TTATGGCCCA TTTCGCGATG CAGTTCAGAG TAATAAAAAG AATTATTTAG ATAAATCAAG CTATCAAATT GATGTGCGTA ATATTAAGGA AGCAATGCTT GAGATTGAGC ATGATATAGC AGAAGGAGCA GATATTGTAA TGGTTAAACC AGGTATGCCG TTTTTGGATG TGATTCGTGA AGCGGCAAAT AATTTTAATG CTAAAATTTT TGCATATCAG GTTAGTGGGG AATACGCAAT GTTAAAATTT GCAGCAGAAA CAGGGGCTAT TGATTGGGAG AGAGCGTTAA TAGAATCATT AATTAGTTTT AAACGTGCCG GTGCAACAGG AATATTTACC TATGCTGCTC TTGAAGTAGC TGAAATATTA AAGCACAATA ACATTAAGTA GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA