SSGCID: Target Page

RiprA.00061.a

UDP-N-acetylglucosamine 2-epimerase

CENTER ID:	RiprA.00061.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00061.a.A1.GE32592	Full length( RiprA.00061.a )		1	376

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272947.5.peg.344
RefSeq:	NP_220717.1
UniProt:	Q9ZDJ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKVMTILGT RPELIKMCLV ISEFDKYTNH ILVHTGQNYA YELNHVFFDD MGIRKPDYFL EIASDNTAKS IGIVIAKVDE VLEKEKPDAV LFYGDTNSCL SAIAAKRRKI PIFHMEAGNR CFDQRVPEEI NRKIIDHISD VNITLTEHAR RYLISEGFPP ERILKSGSHM PEVLNHFMPK ILKSDILEKL LLKAKQYFLI SSHREENVDV KNNLQDLLSS LQILIQEYNY PIIFSTHPRT KKRLKDLECF QELEDKIRFL PPFSFTDYVK LQINAFCILS DSGTLTEEAS ILNLPALNIR EAHERPEGMD AGTLIMSGFK AERILQSVKV ITAQHKNNKC LQNIVSDYAE ASIVSKKILR IVLSYVDYVN RTVWFK

NT Sequence

ATGCTTAAGG TTATGACTAT TCTTGGTACA CGTCCTGAAC TTATTAAAAT GTGTCTAGTG ATTTCAGAAT TTGATAAATA TACTAATCAT ATCTTGGTTC ATACAGGTCA AAATTATGCA TATGAATTAA ATCATGTTTT TTTTGATGAT ATGGGCATTA GAAAACCTGA TTATTTCTTA GAAATAGCAT CAGATAATAC AGCTAAGTCT ATTGGCATTG TTATCGCAAA AGTAGATGAG GTACTTGAAA AAGAAAAACC CGATGCTGTT TTGTTTTATG GTGATACTAA TTCTTGTTTA TCGGCAATTG CAGCGAAACG TCGTAAAATA CCAATTTTCC ATATGGAAGC TGGGAATCGT TGTTTTGATC AAAGAGTACC AGAAGAAATA AATCGTAAAA TTATTGATCA TATTAGTGAT GTGAATATTA CATTAACCGA GCATGCAAGA CGTTATTTAA TTTCTGAAGG GTTTCCTCCT GAGCGTATTT TAAAATCAGG TTCGCATATG CCTGAAGTGC TCAACCATTT CATGCCTAAA ATATTAAAAT CAGATATTTT AGAAAAATTA TTATTAAAAG CAAAGCAGTA TTTCTTAATT AGCTCACATC GTGAAGAAAA TGTAGACGTT AAAAATAATC TTCAAGACTT ATTAAGTAGT TTGCAAATTC TTATTCAAGA GTATAATTAC CCAATAATCT TCTCAACACA TCCAAGGACT AAAAAAAGGC TCAAAGATTT AGAATGTTTC CAGGAACTAG AAGATAAAAT AAGGTTTTTG CCGCCTTTTA GTTTCACAGA TTATGTCAAA CTTCAGATAA ATGCGTTTTG TATTTTGTCT GATAGCGGTA CTCTTACCGA AGAAGCATCT ATACTTAATT TACCTGCTTT AAATATTAGG GAAGCACACG AGCGACCTGA GGGAATGGAT GCGGGAACGC TCATTATGTC TGGTTTTAAA GCAGAACGAA TACTGCAATC GGTAAAAGTC ATTACTGCAC AACACAAAAA TAATAAGTGC TTGCAAAATA TCGTGTCGGA TTATGCTGAA GCAAGTATTG TATCAAAGAA AATATTACGT ATAGTTTTAA GTTATGTTGA TTACGTTAAC CGGACGGTAT GGTTTAAA

Details for RiprA.00061.a.A1.GE32592

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLKVMTILG TRPELIKMCL VISEFDKYTN HILVHTGQNY AYELNHVFFD DMGIRKPDYF LEIASDNTAK SIGIVIAKVD EVLEKEKPDA VLFYGDTNSC LSAIAAKRRK IPIFHMEAGN RCFDQRVPEE INRKIIDHIS DVNITLTEHA RRYLISEGFP PERILKSGSH MPEVLNHFMP KILKSDILEK LLLKAKQYFL ISSHREENVD VKNNLQDLLS SLQILIQEYN YPIIFSTHPR TKKRLKDLEC FQELEDKIRF LPPFSFTDYV KLQINAFCIL SDSGTLTEEA SILNLPALNI REAHERPEGM DAGTLIMSGF KAERILQSVK VITAQHKNNK CLQNIVSDYA EASIVSKKIL RIVLSYVDYV NRTVWFK

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctta aggttatgac tattcttggt acacgtcctg aacttattaa aatgtgtcta gtgatttcag aatttgataa atatactaat catatcttgg ttcatacagg tcaaaattat gcatatgaat taaatcatgt tttttttgat gatatgggca ttagaaaacc tgattatttc ttagaaatag catcagataa tacagctaag tctattggca ttgttatcgc aaaagtagat gaggtacttg aaaaagaaaa acccgatgct gttttgtttt atggtgatac taattcttgt ttatcggcaa ttgcagcgaa acgtcgtaaa ataccaattt tccatatgga agctgggaat cgttgttttg atcaaagagt accagaagaa ataaatcgta aaattattga tcatattagt gatgtgaata ttacattaac cgagcatgca agacgttatt taatttctga agggtttcct cctgagcgta ttttaaaatc aggttcgcat atgcctgaag tgctcaacca tttcatgcct aaaatattaa aatcagatat tttagaaaaa ttattattaa aagcaaagca gtatttctta attagctcac atcgtgaaga aaatgtagac gttaaaaata atcttcaaga cttattaagt agtttgcaaa ttcttattca agagtataat tacccaataa tcttctcaac acatccaagg actaaaaaaa ggctcaaaga tttagaatgt ttccaggaac tagaagataa aataaggttt ttgccgcctt ttagtttcac agattatgtc aaacttcaga taaatgcgtt ttgtattttg tctgatagcg gtactcttac cgaagaagca tctatactta atttacctgc tttaaatatt agggaagcac acgagcgacc tgagggaatg gatgcgggaa cgctcattat gtctggtttt aaagcagaac gaatactgca atcggtaaaa gtcattactg cacaacacaa aaataataag tgcttgcaaa atatcgtgtc ggattatgct gaagcaagta ttgtatcaaa gaaaatatta cgtatagttt taagttatgt tgattacgtt aaccggacgg tatggtttaa ataataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttaaggtt atgactattc ttggtacacg tcctgaactt attaaaatgt gtctagtgat ttcagaattt gataaatata ctaatcatat cttggttcat acaggtcaaa attatgcata tgaattaaat catgtttttt ttgatgatat gggcattaga aaacctgatt atttcttaga aatagcatca gataatacag ctaagtctat tggcattgtt atcgcaaaag tagatgaggt acttgaaaaa gaaaaacccg atgctgtttt gttttatggt gatactaatt cttgtttatc ggcaattgca gcgaaacgtc gtaaaatacc aattttccat atggaagctg ggaatcgttg ttttgatcaa agagtaccag aagaaataaa tcgtaaaatt attgatcata ttagtgatgt gaatattaca ttaaccgagc atgcaagacg ttatttaatt tctgaagggt ttcctcctga gcgtatttta aaatcaggtt cgcatatgcc tgaagtgctc aaccatttca tgcctaaaat attaaaatca gatattttag aaaaattatt attaaaagca aagcagtatt tcttaattag ctcacatcgt gaagaaaatg tagacgttaa aaataatctt caagacttat taagtagttt gcaaattctt attcaagagt ataattaccc aataatcttc tcaacacatc caaggactaa aaaaaggctc aaagatttag aatgtttcca ggaactagaa gataaaataa ggtttttgcc gccttttagt ttcacagatt atgtcaaact tcagataaat gcgttttgta ttttgtctga tagcggtact cttaccgaag aagcatctat acttaattta cctgctttaa atattaggga agcacacgag cgacctgagg gaatggatgc gggaacgctc attatgtctg gttttaaagc agaacgaata ctgcaatcgg taaaagtcat tactgcacaa cacaaaaata ataagtgctt gcaaaatatc gtgtcggatt atgctgaagc aagtattgta tcaaagaaaa tattacgtat agttttaagt tatgttgatt acgttaaccg gacggtatgg tttaaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa