RiprA.00057.a

ABC TRANSPORTER ATP-BINDING PROTEIN (abcT2)

CENTER ID:	RiprA.00057.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00057.a.B1.GE27182	Full length( RiprA.00057.a )		1	254

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_132803
PATRIC ID:	fig\|272947.5.peg.517
RefSeq:	NP_220884.1
UniProt:	Q9ZD39

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVKWRYYRKH INKKMDSLQI KNISKSYKKR NILTDISLHI KQGEIVGLFG PNGAGKTTCF NIIIGLMRPD SGQLLLNDIN ITNLPIYLRA RLGIGYLLQE PSIFRGLSVA DNIKAVVEIS ENDKEVIEQK THNLLEKFSI VHLKDLSSAS LSGGERRRLE IARSFVIEPK FIMLDEPLAG IDPLAISDIK KLITYLREFN IGILITDHNV RDTLDIVDRA YVIFEGKVLL EGSAQEIANS KKVKEVYLGE SFNF

NT Sequence

GTGGTTAAAT GGAGGTATTA CCGGAAACAC ATAAATAAAA AGATGGATAG TTTACAAATT AAAAATATAT CAAAATCCTA TAAAAAAAGA AATATATTAA CTGATATATC TCTTCATATA AAACAAGGAG AGATAGTTGG TTTATTTGGC CCTAACGGAG CAGGAAAAAC AACTTGTTTT AACATTATTA TTGGTTTAAT GCGACCAGAC TCAGGACAAT TACTTTTAAA TGATATAAAT ATTACAAACT TGCCTATTTA TTTACGAGCT AGGCTTGGAA TCGGATATCT CCTTCAAGAA CCTTCAATAT TTCGTGGATT ATCAGTTGCA GATAATATTA AGGCTGTAGT TGAAATATCT GAAAATGATA AAGAAGTAAT AGAACAAAAA ACACATAATT TGCTAGAGAA ATTTTCAATA GTACATTTAA AAGATTTGTC CTCTGCTAGC TTATCAGGCG GGGAGAGACG TAGACTTGAG ATTGCGCGCT CATTTGTAAT AGAACCTAAA TTTATAATGC TTGATGAACC ACTTGCAGGT ATTGATCCGC TTGCTATTTC TGATATCAAA AAGCTCATTA CTTATTTACG TGAATTTAAT ATAGGTATAT TAATAACCGA TCATAATGTA CGTGATACTT TAGATATAGT AGACCGTGCT TATGTTATTT TTGAAGGTAA AGTATTATTA GAAGGAAGTG CTCAAGAAAT AGCGAACAGT AAGAAAGTCA AAGAAGTATA TTTAGGCGAG AGTTTTAATT TT

Details for RiprA.00057.a.B1.GE27182

HARVESTED ON:	8/17/2009
SEQUENCED ON:	9/29/2009
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMV VKWXYYRKHI NKKMDSLQIK NISXSYKKRN ILTDISLHIK QGEIVGLFGP XGXXKXXCXN IIIGLMXPDS GQLLXXDINI TXLXIYLXAX LXIGYLXQEP SIFRGLSXXX XIKXVVEISE NDXEVIEQKT HNLLEKFSIV HLKDLSSASL SGGERRRLEI ARSFVIEXKF IMLDEPLAGI DPLAIXDIKK LITYLREFNI GILITDHNVR DTLDIVDRAY VIFEGKVLLE GSAQEIANSK KVKEVYLGES FNX

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMV VKWRYYRKHI NKKMDSLQIK NISKSYKKRN ILTDISLHIK QGEIVGLFGP NGAGKTTCFN IIIGLMRPDS GQLLLNDINI TNLPIYLRAR LGIGYLLQEP SIFRGLSVAD NIKAVVEISE NDKEVIEQKT HNLLEKFSIV HLKDLSSASL SGGERRRLEI ARSFVIEPKF IMLDEPLAGI DPLAISDIKK LITYLREFNI GILITDHNVR DTLDIVDRAY VIFEGKVLLE GSAQEIANSK KVKEVYLGES FNF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgg ttaaatggag gtattaccgg aaacacataa ataaaaagat ggatagttta caaattaaaa atatatcaaa atcctataaa aaaagaaata tattaactga tatatctctt catataaaac aaggagagat agttggttta tttggcccta acggagcagg aaaaacaact tgttttaaca ttattattgg tttaatgcga ccagactcag gacaattact tttaaatgat ataaatatta caaacttgcc tatttattta cgagctaggc ttggaatcgg atatctcctt caagaacctt caatatttcg tggattatca gttgcagata atattaaggc tgtagttgaa atatctgaaa atgataaaga agtaatagaa caaaaaacac ataatttgct agagaaattt tcaatagtac atttaaaaga tttgtcctct gctagcttat caggcgggga gagacgtaga cttgagattg cgcgctcatt tgtaatagaa cctaaattta taatgcttga tgaaccactt gcaggtattg atccgcttgc tatttctgat atcaaaaagc tcattactta tttacgtgaa tttaatatag gtatattaat aaccgatcat aatgtacgtg atactttaga tatagtagac cgtgcttatg ttatttttga aggtaaagta ttattagaag gaagtgctca agaaatagcg aacagtaaga aagtcaaaga agtatattta ggcgagagtt ttaatttttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat