RiprA.00053.a

ATP-dependent protease ATP-binding subunit

CENTER ID: RiprA.00053.a
ORGANISM: Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE: Endemic typhus
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiprA.00053.a.A1.GE25413 Full length( RiprA.00053.a ) 1 450
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiprA.00053.a.A1.PS00068 Full length( RiprA.00053.a ) 1 450
RiprA.00053.a.A1.PS00087 Full length( RiprA.00053.a ) 1 450
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129971
PATRIC ID: fig|272947.5.peg.329
RefSeq: NP_220703
UniProt: Q9ZDK8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSTETTYKK DNMGLTPSQI VNELNRFIVG QDKAKKAVAI ALRNRYRRKR VEGNLRNEIV PKNILMIGST GVGKTEIARR LAKLTNSPFY KIEATKFTEV GYVGRDVESI IRDLVEIAVN TEKALAKIAV DINAREKAIE RILDSLVGKT SSSETREKFK AKVLNGELDD TEIEISVADT TPVGGGGFEI PCIPGASMGV LNLGDMIGRA LGNSKTKTKK MLIKDAMTVI IPEESEKLID QEKIIQKAIN LAENDGIVFI DEIDKIASTS NAGAKNAEIS REGVQRDLLP LIEGTTVNTK YGPVKTDHIL FIASGAFHIA KPSDLLPELQ GRLPIRVELN LLTKDDMIKI LLEPETSLIK QYSALIGTEE VHLEFTDAAI EKIADYAITV NLEVEDIGAR RLHTILENLL EDISFEASEM KVKKIIIDDK FVENQLSKII TNLDLAKFVL
NT Sequence
ATGAAATCTA CTGAAACTAC TTATAAAAAA GACAATATGG GGCTTACCCC TTCTCAAATA GTTAATGAAC TTAACAGATT TATCGTAGGT CAAGACAAGG CCAAAAAAGC TGTAGCTATT GCACTTAGAA ATCGTTATCG TCGTAAAAGA GTCGAAGGCA ATTTACGTAA TGAAATAGTA CCAAAAAATA TTTTAATGAT TGGTTCAACT GGTGTCGGCA AAACAGAAAT AGCAAGACGG CTTGCAAAAT TGACTAATTC GCCTTTTTAC AAAATAGAAG CAACTAAATT TACTGAAGTA GGCTACGTTG GGCGTGATGT AGAATCAATA ATTCGTGATT TAGTAGAAAT AGCTGTTAAT ACTGAAAAAG CTTTAGCAAA AATAGCAGTA GATATTAATG CTCGTGAAAA AGCGATAGAG AGAATATTAG ATAGTTTAGT AGGAAAAACT TCTAGTAGCG AGACTAGAGA GAAATTTAAA GCAAAAGTTT TAAACGGTGA ACTTGATGAT ACGGAAATCG AAATTAGTGT CGCTGATACT ACACCTGTTG GTGGTGGAGG TTTTGAAATA CCGTGCATAC CTGGAGCATC TATGGGTGTT CTTAATCTTG GCGATATGAT TGGCAGAGCA TTGGGCAATA GTAAGACTAA AACAAAAAAA ATGCTAATTA AAGATGCTAT GACTGTTATT ATACCTGAGG AATCAGAAAA ATTAATAGAC CAAGAAAAAA TCATTCAGAA AGCTATAAAT TTAGCTGAAA ATGACGGTAT AGTTTTTATT GACGAAATTG ATAAAATAGC TTCAACCAGT AATGCAGGAG CAAAAAATGC CGAAATAAGT CGGGAAGGTG TACAACGAGA TTTATTACCT TTGATAGAAG GTACAACGGT TAATACTAAA TATGGGCCGG TTAAAACCGA TCATATATTA TTTATTGCCT CAGGTGCTTT TCATATTGCT AAACCTTCTG ATTTATTACC AGAGTTGCAA GGAAGGTTAC CGATTAGAGT AGAATTAAAT TTGCTCACTA AAGATGATAT GATTAAAATA TTGCTTGAAC CTGAAACTAG TTTAATAAAG CAATATTCGG CATTGATAGG TACTGAAGAA GTACACCTTG AATTTACAGA TGCTGCTATT GAAAAGATAG CTGATTATGC AATCACTGTT AATTTAGAAG TAGAAGATAT AGGGGCTAGA AGATTGCATA CTATACTTGA GAATTTACTT GAGGATATAA GCTTTGAAGC TAGTGAAATG AAAGTCAAAA AAATAATTAT CGATGATAAA TTTGTGGAAA ATCAATTATC GAAGATAATC ACCAATCTTG ACTTAGCTAA GTTTGTTCTA
Details for RiprA.00053.a.A1.GE25413
HARVESTED ON: 6/30/2008
SEQUENCED ON: 3/6/2009
EXPECTED MW: 52kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKSTETTYK KDNMGLTPSQ IVNELNRFIV GQDKAKKAVA IALRNRYRRK RVEGNLRNEI VPKNILMIGS TGVGKTEIAR RLAKLTNSPF YKIEATKFTE VGYVGRDVES IIRDLVEIAV NTEKALAKIA VDINAREKAI ERILDSLVGK TSSSETREKF KAKVLNGELD DTEIEISVAD TTPVGGGGFE IPCIPGASMG VLNLGDMIGR ALGNSKTKTK KMLIKDAMTV IIPEESEKLI DQEKIIQKAI NLAENDGIVF IDEIDKIAST SNAGAKNAEI SREGVQRDLL PLIEGTTVNT KYGPVKTDHI LFIASGAFHI AKPSDLLPEL QGRLPIRVEL NLLTKDDMIK ILLEPETSLI KQYSALIGTE EVHLEFTDAA IEKIADYAIT VNLEVEDIGA RRLHTILENL LEDISFEASE MKVKKIIIDD KFVENQLSKI ITNLDLAKFV L
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat ctactgaaac tacttataaa aaagacaata tggggcttac cccttctcaa atagttaatg aacttaacag atttatcgta ggtcaagaca aggccaaaaa agctgtagct attgcactta gaaatcgtta tcgtcgtaaa agagtcgaag gcaatttacg taatgaaata gtaccaaaaa atattttaat gattggttca actggtgtcg gcaaaacaga aatagcaaga cggcttgcaa aattgactaa ttcgcctttt tacaaaatag aagcaactaa atttactgaa gtaggctacg ttgggcgtga tgtagaatca ataattcgtg atttagtaga aatagctgtt aatactgaaa aagctttagc aaaaatagca gtagatatta atgctcgtga aaaagcgata gagagaatat tagatagttt agtaggaaaa acttctagta gcgagactag agagaaattt aaagcaaaag ttttaaacgg tgaacttgat gatacggaaa tcgaaattag tgtcgctgat actacacctg ttggtggtgg aggttttgaa ataccgtgca tacctggagc atctatgggt gttcttaatc ttggcgatat gattggcaga gcattgggca atagtaagac taaaacaaaa aaaatgctaa ttaaagatgc tatgactgtt attatacctg aggaatcaga aaaattaata gaccaagaaa aaatcattca gaaagctata aatttagctg aaaatgacgg tatagttttt attgacgaaa ttgataaaat agcttcaacc agtaatgcag gagcaaaaaa tgccgaaata agtcgggaag gtgtacaacg agatttatta cctttgatag aaggtacaac ggttaatact aaatatgggc cggttaaaac cgatcatata ttatttattg cctcaggtgc ttttcatatt gctaaacctt ctgatttatt accagagttg caaggaaggt taccgattag agtagaatta aatttgctca ctaaagatga tatgattaaa atattgcttg aacctgaaac tagtttaata aagcaatatt cggcattgat aggtactgaa gaagtacacc ttgaatttac agatgctgct attgaaaaga tagctgatta tgcaatcact gttaatttag aagtagaaga tataggggct agaagattgc atactatact tgagaattta cttgaggata taagctttga agctagtgaa atgaaagtca aaaaaataat tatcgatgat aaatttgtgg aaaatcaatt atcgaagata atcaccaatc ttgacttagc taagtttgtt ctat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKSTETTYK KDNMGLTPSQ IVNELNRFIV GQDKAKKAVA IALRNRYRRK RVEGNLRNEI VPKNILMIGS TGVGKTEIAR RLAKLTNSPF YKIEATKFTE VGYVGRDVES IIRDLVEIAV NTEKALAKIA VDINAREKAI ERILDSLVGK TSSSETREKF KAKVLNGELD DTEIEISVAD TTPVGGGGFE IPCIPGASMG VLNLGDMIGR ALGNSKTKTK KMLIKDAMTV IIPEESEKLI DQEKIIQKAI NLAENDGIVF IDEIDKIAST SNAGAKNAEI SREGVQRDLL PLIEGTTVNT KYGPVKTDHI LFIASGAFHI AKPSDLLPEL QGRLPIRVEL NLLTKDDMIK ILLEPETSLI KQYSALIGTE EVHLEFTDAA IEKIADYAIT VNLEVEDIGA RRLHTILENL LEDISFEASE MKVKKIIIDD KFVENQLSKI ITNLDLAKFV L
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaatctact gaaactactt ataaaaaaga caatatgggg cttacccctt ctcaaatagt taatgaactt aacagattta tcgtaggtca agacaaggcc aaaaaagctg tagctattgc acttagaaat cgttatcgtc gtaaaagagt cgaaggcaat ttacgtaatg aaatagtacc aaaaaatatt ttaatgattg gttcaactgg tgtcggcaaa acagaaatag caagacggct tgcaaaattg actaattcgc ctttttacaa aatagaagca actaaattta ctgaagtagg ctacgttggg cgtgatgtag aatcaataat tcgtgattta gtagaaatag ctgttaatac tgaaaaagct ttagcaaaaa tagcagtaga tattaatgct cgtgaaaaag cgatagagag aatattagat agtttagtag gaaaaacttc tagtagcgag actagagaga aatttaaagc aaaagtttta aacggtgaac ttgatgatac ggaaatcgaa attagtgtcg ctgatactac acctgttggt ggtggaggtt ttgaaatacc gtgcatacct ggagcatcta tgggtgttct taatcttggc gatatgattg gcagagcatt gggcaatagt aagactaaaa caaaaaaaat gctaattaaa gatgctatga ctgttattat acctgaggaa tcagaaaaat taatagacca agaaaaaatc attcagaaag ctataaattt agctgaaaat gacggtatag tttttattga cgaaattgat aaaatagctt caaccagtaa tgcaggagca aaaaatgccg aaataagtcg ggaaggtgta caacgagatt tattaccttt gatagaaggt acaacggtta atactaaata tgggccggtt aaaaccgatc atatattatt tattgcctca ggtgcttttc atattgctaa accttctgat ttattaccag agttgcaagg aaggttaccg attagagtag aattaaattt gctcactaaa gatgatatga ttaaaatatt gcttgaacct gaaactagtt taataaagca atattcggca ttgataggta ctgaagaagt acaccttgaa tttacagatg ctgctattga aaagatagct gattatgcaa tcactgttaa tttagaagta gaagatatag gggctagaag attgcatact atacttgaga atttacttga ggatataagc tttgaagcta gtgaaatgaa agtcaaaaaa ataattatcg atgataaatt tgtggaaaat caattatcga agataatcac caatcttgac ttagctaagt ttgttctata gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for RiprA.00053.a.A1.PS00068
PURIFICATION DATe: 9/9/2008
CONCENTRATION: 39.75mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKSTETTYK KDNMGLTPSQ IVNELNRFIV GQDKAKKAVA IALRNRYRRK RVEGNLRNEI VPKNILMIGS TGVGKTEIAR RLAKLTNSPF YKIEATKFTE VGYVGRDVES IIRDLVEIAV NTEKALAKIA VDINAREKAI ERILDSLVGK TSSSETREKF KAKVLNGELD DTEIEISVAD TTPVGGGGFE IPCIPGASMG VLNLGDMIGR ALGNSKTKTK KMLIKDAMTV IIPEESEKLI DQEKIIQKAI NLAENDGIVF IDEIDKIAST SNAGAKNAEI SREGVQRDLL PLIEGTTVNT KYGPVKTDHI LFIASGAFHI AKPSDLLPEL QGRLPIRVEL NLLTKDDMIK ILLEPETSLI KQYSALIGTE EVHLEFTDAA IEKIADYAIT VNLEVEDIGA RRLHTILENL LEDISFEASE MKVKKIIIDD KFVENQLSKI ITNLDLAKFV L
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat ctactgaaac tacttataaa aaagacaata tggggcttac cccttctcaa atagttaatg aacttaacag atttatcgta ggtcaagaca aggccaaaaa agctgtagct attgcactta gaaatcgtta tcgtcgtaaa agagtcgaag gcaatttacg taatgaaata gtaccaaaaa atattttaat gattggttca actggtgtcg gcaaaacaga aatagcaaga cggcttgcaa aattgactaa ttcgcctttt tacaaaatag aagcaactaa atttactgaa gtaggctacg ttgggcgtga tgtagaatca ataattcgtg atttagtaga aatagctgtt aatactgaaa aagctttagc aaaaatagca gtagatatta atgctcgtga aaaagcgata gagagaatat tagatagttt agtaggaaaa acttctagta gcgagactag agagaaattt aaagcaaaag ttttaaacgg tgaacttgat gatacggaaa tcgaaattag tgtcgctgat actacacctg ttggtggtgg aggttttgaa ataccgtgca tacctggagc atctatgggt gttcttaatc ttggcgatat gattggcaga gcattgggca atagtaagac taaaacaaaa aaaatgctaa ttaaagatgc tatgactgtt attatacctg aggaatcaga aaaattaata gaccaagaaa aaatcattca gaaagctata aatttagctg aaaatgacgg tatagttttt attgacgaaa ttgataaaat agcttcaacc agtaatgcag gagcaaaaaa tgccgaaata agtcgggaag gtgtacaacg agatttatta cctttgatag aaggtacaac ggttaatact aaatatgggc cggttaaaac cgatcatata ttatttattg cctcaggtgc ttttcatatt gctaaacctt ctgatttatt accagagttg caaggaaggt taccgattag agtagaatta aatttgctca ctaaagatga tatgattaaa atattgcttg aacctgaaac tagtttaata aagcaatatt cggcattgat aggtactgaa gaagtacacc ttgaatttac agatgctgct attgaaaaga tagctgatta tgcaatcact gttaatttag aagtagaaga tataggggct agaagattgc atactatact tgagaattta cttgaggata taagctttga agctagtgaa atgaaagtca aaaaaataat tatcgatgat aaatttgtgg aaaatcaatt atcgaagata atcaccaatc ttgacttagc taagtttgtt ctat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKSTETTYK KDNMGLTPSQ IVNELNRFIV GQDKAKKAVA IALRNRYRRK RVEGNLRNEI VPKNILMIGS TGVGKTEIAR RLAKLTNSPF YKIEATKFTE VGYVGRDVES IIRDLVEIAV NTEKALAKIA VDINAREKAI ERILDSLVGK TSSSETREKF KAKVLNGELD DTEIEISVAD TTPVGGGGFE IPCIPGASMG VLNLGDMIGR ALGNSKTKTK KMLIKDAMTV IIPEESEKLI DQEKIIQKAI NLAENDGIVF IDEIDKIAST SNAGAKNAEI SREGVQRDLL PLIEGTTVNT KYGPVKTDHI LFIASGAFHI AKPSDLLPEL QGRLPIRVEL NLLTKDDMIK ILLEPETSLI KQYSALIGTE EVHLEFTDAA IEKIADYAIT VNLEVEDIGA RRLHTILENL LEDISFEASE MKVKKIIIDD KFVENQLSKI ITNLDLAKFV L
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATCTACT GAAACTACTT ATAAAAAAGA CAATATGGGG CTTACCCCTT CTCAAATAGT TAATGAACTT AACAGATTTA TCGTAGGTCA AGACAAGGCC AAAAAAGCTG TAGCTATTGC ACTTAGAAAT CGTTATCGTC GTAAAAGAGT CGAAGGCAAT TTACGTAATG AAATAGTACC AAAAAATATT TTAATGATTG GTTCAACTGG TGTCGGCAAA ACAGAAATAG CAAGACGGCT TGCAAAATTG ACTAATTCGC CTTTTTACAA AATAGAAGCA ACTAAATTTA CTGAAGTAGG CTACGTTGGG CGTGATGTAG AATCAATAAT TCGTGATTTA GTAGAAATAG CTGTTAATAC TGAAAAAGCT TTAGCAAAAA TAGCAGTAGA TATTAATGCT CGTGAAAAAG CGATAGAGAG AATATTAGAT AGTTTAGTAG GAAAAACTTC TAGTAGCGAG ACTAGAGAGA AATTTAAAGC AAAAGTTTTA AACGGTGAAC TTGATGATAC GGAAATCGAA ATTAGTGTCG CTGATACTAC ACCTGTTGGT GGTGGAGGTT TTGAAATACC GTGCATACCT GGAGCATCTA TGGGTGTTCT TAATCTTGGC GATATGATTG GCAGAGCATT GGGCAATAGT AAGACTAAAA CAAAAAAAAT GCTAATTAAA GATGCTATGA CTGTTATTAT ACCTGAGGAA TCAGAAAAAT TAATAGACCA AGAAAAAATC ATTCAGAAAG CTATAAATTT AGCTGAAAAT GACGGTATAG TTTTTATTGA CGAAATTGAT AAAATAGCTT CAACCAGTAA TGCAGGAGCA AAAAATGCCG AAATAAGTCG GGAAGGTGTA CAACGAGATT TATTACCTTT GATAGAAGGT ACAACGGTTA ATACTAAATA TGGGCCGGTT AAAACCGATC ATATATTATT TATTGCCTCA GGTGCTTTTC ATATTGCTAA ACCTTCTGAT TTATTACCAG AGTTGCAAGG AAGGTTACCG ATTAGAGTAG AATTAAATTT GCTCACTAAA GATGATATGA TTAAAATATT GCTTGAACCT GAAACTAGTT TAATAAAGCA ATATTCGGCA TTGATAGGTA CTGAAGAAGT ACACCTTGAA TTTACAGATG CTGCTATTGA AAAGATAGCT GATTATGCAA TCACTGTTAA TTTAGAAGTA GAAGATATAG GGGCTAGAAG ATTGCATACT ATACTTGAGA ATTTACTTGA GGATATAAGC TTTGAAGCTA GTGAAATGAA AGTCAAAAAA ATAATTATCG ATGATAAATT TGTGGAAAAT CAATTATCGA AGATAATCAC CAATCTTGAC TTAGCTAAGT TTGTTCTATA GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for RiprA.00053.a.A1.PS00087
PURIFICATION DATe: 10/14/2008
CONCENTRATION: 12.47mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKSTETTYK KDNMGLTPSQ IVNELNRFIV GQDKAKKAVA IALRNRYRRK RVEGNLRNEI VPKNILMIGS TGVGKTEIAR RLAKLTNSPF YKIEATKFTE VGYVGRDVES IIRDLVEIAV NTEKALAKIA VDINAREKAI ERILDSLVGK TSSSETREKF KAKVLNGELD DTEIEISVAD TTPVGGGGFE IPCIPGASMG VLNLGDMIGR ALGNSKTKTK KMLIKDAMTV IIPEESEKLI DQEKIIQKAI NLAENDGIVF IDEIDKIAST SNAGAKNAEI SREGVQRDLL PLIEGTTVNT KYGPVKTDHI LFIASGAFHI AKPSDLLPEL QGRLPIRVEL NLLTKDDMIK ILLEPETSLI KQYSALIGTE EVHLEFTDAA IEKIADYAIT VNLEVEDIGA RRLHTILENL LEDISFEASE MKVKKIIIDD KFVENQLSKI ITNLDLAKFV L
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat ctactgaaac tacttataaa aaagacaata tggggcttac cccttctcaa atagttaatg aacttaacag atttatcgta ggtcaagaca aggccaaaaa agctgtagct attgcactta gaaatcgtta tcgtcgtaaa agagtcgaag gcaatttacg taatgaaata gtaccaaaaa atattttaat gattggttca actggtgtcg gcaaaacaga aatagcaaga cggcttgcaa aattgactaa ttcgcctttt tacaaaatag aagcaactaa atttactgaa gtaggctacg ttgggcgtga tgtagaatca ataattcgtg atttagtaga aatagctgtt aatactgaaa aagctttagc aaaaatagca gtagatatta atgctcgtga aaaagcgata gagagaatat tagatagttt agtaggaaaa acttctagta gcgagactag agagaaattt aaagcaaaag ttttaaacgg tgaacttgat gatacggaaa tcgaaattag tgtcgctgat actacacctg ttggtggtgg aggttttgaa ataccgtgca tacctggagc atctatgggt gttcttaatc ttggcgatat gattggcaga gcattgggca atagtaagac taaaacaaaa aaaatgctaa ttaaagatgc tatgactgtt attatacctg aggaatcaga aaaattaata gaccaagaaa aaatcattca gaaagctata aatttagctg aaaatgacgg tatagttttt attgacgaaa ttgataaaat agcttcaacc agtaatgcag gagcaaaaaa tgccgaaata agtcgggaag gtgtacaacg agatttatta cctttgatag aaggtacaac ggttaatact aaatatgggc cggttaaaac cgatcatata ttatttattg cctcaggtgc ttttcatatt gctaaacctt ctgatttatt accagagttg caaggaaggt taccgattag agtagaatta aatttgctca ctaaagatga tatgattaaa atattgcttg aacctgaaac tagtttaata aagcaatatt cggcattgat aggtactgaa gaagtacacc ttgaatttac agatgctgct attgaaaaga tagctgatta tgcaatcact gttaatttag aagtagaaga tataggggct agaagattgc atactatact tgagaattta cttgaggata taagctttga agctagtgaa atgaaagtca aaaaaataat tatcgatgat aaatttgtgg aaaatcaatt atcgaagata atcaccaatc ttgacttagc taagtttgtt ctat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKSTETTYK KDNMGLTPSQ IVNELNRFIV GQDKAKKAVA IALRNRYRRK RVEGNLRNEI VPKNILMIGS TGVGKTEIAR RLAKLTNSPF YKIEATKFTE VGYVGRDVES IIRDLVEIAV NTEKALAKIA VDINAREKAI ERILDSLVGK TSSSETREKF KAKVLNGELD DTEIEISVAD TTPVGGGGFE IPCIPGASMG VLNLGDMIGR ALGNSKTKTK KMLIKDAMTV IIPEESEKLI DQEKIIQKAI NLAENDGIVF IDEIDKIAST SNAGAKNAEI SREGVQRDLL PLIEGTTVNT KYGPVKTDHI LFIASGAFHI AKPSDLLPEL QGRLPIRVEL NLLTKDDMIK ILLEPETSLI KQYSALIGTE EVHLEFTDAA IEKIADYAIT VNLEVEDIGA RRLHTILENL LEDISFEASE MKVKKIIIDD KFVENQLSKI ITNLDLAKFV L
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATCTACT GAAACTACTT ATAAAAAAGA CAATATGGGG CTTACCCCTT CTCAAATAGT TAATGAACTT AACAGATTTA TCGTAGGTCA AGACAAGGCC AAAAAAGCTG TAGCTATTGC ACTTAGAAAT CGTTATCGTC GTAAAAGAGT CGAAGGCAAT TTACGTAATG AAATAGTACC AAAAAATATT TTAATGATTG GTTCAACTGG TGTCGGCAAA ACAGAAATAG CAAGACGGCT TGCAAAATTG ACTAATTCGC CTTTTTACAA AATAGAAGCA ACTAAATTTA CTGAAGTAGG CTACGTTGGG CGTGATGTAG AATCAATAAT TCGTGATTTA GTAGAAATAG CTGTTAATAC TGAAAAAGCT TTAGCAAAAA TAGCAGTAGA TATTAATGCT CGTGAAAAAG CGATAGAGAG AATATTAGAT AGTTTAGTAG GAAAAACTTC TAGTAGCGAG ACTAGAGAGA AATTTAAAGC AAAAGTTTTA AACGGTGAAC TTGATGATAC GGAAATCGAA ATTAGTGTCG CTGATACTAC ACCTGTTGGT GGTGGAGGTT TTGAAATACC GTGCATACCT GGAGCATCTA TGGGTGTTCT TAATCTTGGC GATATGATTG GCAGAGCATT GGGCAATAGT AAGACTAAAA CAAAAAAAAT GCTAATTAAA GATGCTATGA CTGTTATTAT ACCTGAGGAA TCAGAAAAAT TAATAGACCA AGAAAAAATC ATTCAGAAAG CTATAAATTT AGCTGAAAAT GACGGTATAG TTTTTATTGA CGAAATTGAT AAAATAGCTT CAACCAGTAA TGCAGGAGCA AAAAATGCCG AAATAAGTCG GGAAGGTGTA CAACGAGATT TATTACCTTT GATAGAAGGT ACAACGGTTA ATACTAAATA TGGGCCGGTT AAAACCGATC ATATATTATT TATTGCCTCA GGTGCTTTTC ATATTGCTAA ACCTTCTGAT TTATTACCAG AGTTGCAAGG AAGGTTACCG ATTAGAGTAG AATTAAATTT GCTCACTAAA GATGATATGA TTAAAATATT GCTTGAACCT GAAACTAGTT TAATAAAGCA ATATTCGGCA TTGATAGGTA CTGAAGAAGT ACACCTTGAA TTTACAGATG CTGCTATTGA AAAGATAGCT GATTATGCAA TCACTGTTAA TTTAGAAGTA GAAGATATAG GGGCTAGAAG ATTGCATACT ATACTTGAGA ATTTACTTGA GGATATAAGC TTTGAAGCTA GTGAAATGAA AGTCAAAAAA ATAATTATCG ATGATAAATT TGTGGAAAAT CAATTATCGA AGATAATCAC CAATCTTGAC TTAGCTAAGT TTGTTCTATA GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA