RiprA.00047.a
fumarate hydratase
| CENTER ID: | RiprA.00047.a |
| ORGANISM: | Rickettsia prowazekii str. Madrid E |
| ASSOCIATED DISEASE: | Endemic typhus |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiprA.00047.a.A1.GE25412 | Full length( RiprA.00047.a ) | 1 | 461 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiprA.00047.a.A1.PS00079 | Full length( RiprA.00047.a ) | 1 | 461 | ||
| RiprA.00047.a.A1.PS00090 | Full length( RiprA.00047.a ) | 1 | 461 |
Structures
3GTD
DEPOSITED: 3/27/2009
DETERMINATION: XRay
CLONE: RiprA.00047.a.A1.GE25412
PROTEIN: RiprA.00047.a.A1.PS00079
Publications by SSGCID
Structure of fumarate hydratase from Rickettsia prowazekii, the agent of typhus and suspected relative of the mitochondria.
Edwards TE, Guo W, Myler PJ, Olsen C, Phan I, Stewart LJ, Subramanian S, Van Voorhis WC, Zhang Y
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1123-8
PMID: 21904061; PMCID: PMC3169413
PDB:
3GTD
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127258 |
| PATRIC ID: | fig|272947.5.peg.685 |
| RefSeq: | NP_221027 |
| UniProt: | Q9ZCQ4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNYRIESDS FGEIQIEEKF YWGAQTQRSL NNFKISKQKM PKILIRALAI LKKCAAQVNY EFGDLEYKIA TSIDKAIDRI LAGEFEDNFP LVVWQTGSGT QTNMNMNEVI ASIANEELTG KKGGKFPVHP NDHVNKGQSS NDSFPTAMHI ATVLATKQQL IPALNNLLTY LQDKSKDWDK IIKIGRTHLQ DATPLTLKQE FSGYITQIEY ALERIEDALK KVYLLAQGGT AVGTGINSKI GFDIKFAQKV AEFTQQPFKT APNKFESLAA HDALVEFSGT LNTIAVSLMK IANDIRLLGS GPRCGLGELH LPENEPGSSI MPGKVNPTQV EALTMVCTQV MGNHVTVTIA GSNGHLELNV FKPVIIYNIL QSIELLSDSV NSFVTHCVKG LEPNIARINT LRDKSLMLVT VLNPHIGYDN AAKIAKEAHK YGITLKEAAK KLNFLSEEEF DKIVVPEKMI S
NT Sequence
ATGAAAAATT ACAGAATCGA AAGTGACTCT TTTGGAGAAA TTCAAATAGA AGAAAAATTT TATTGGGGTG CTCAAACTCA AAGATCTTTA AACAATTTTA AAATAAGCAA GCAAAAAATG CCAAAAATTT TAATTCGTGC TCTTGCTATT TTAAAAAAAT GTGCTGCACA GGTTAATTAC GAGTTTGGTG ATTTAGAATA TAAAATAGCT ACAAGTATAG ATAAAGCAAT TGATAGAATC CTAGCAGGCG AATTTGAAGA TAATTTTCCT TTAGTAGTTT GGCAAACAGG GTCAGGTACA CAAACAAACA TGAATATGAA CGAGGTTATT GCATCTATTG CTAATGAAGA ATTAACTGGT AAGAAAGGTG GTAAATTCCC AGTACATCCT AACGATCATG TTAATAAAGG GCAATCATCC AACGACTCTT TTCCAACAGC TATGCATATA GCAACTGTAC TTGCAACTAA ACAGCAGCTT ATACCAGCTT TGAATAATTT ACTTACATAT TTGCAAGACA AGTCAAAAGA TTGGGATAAA ATCATAAAAA TAGGACGCAC TCACTTGCAG GATGCAACTC CTTTAACACT TAAACAGGAA TTCTCAGGAT ATATTACACA AATAGAGTAT GCTTTAGAAC GCATAGAAGA TGCACTAAAA AAAGTTTATC TACTTGCTCA AGGCGGAACA GCAGTCGGTA CAGGTATTAA TTCAAAAATA GGCTTTGATA TAAAATTTGC TCAAAAAGTT GCAGAGTTTA CTCAACAACC ATTTAAAACT GCTCCTAATA AATTTGAAAG TTTAGCAGCA CATGATGCAC TAGTAGAATT TTCTGGAACT CTTAATACTA TAGCAGTTAG CTTAATGAAA ATAGCAAATG ATATAAGATT GCTCGGCTCC GGTCCAAGAT GCGGTCTTGG TGAACTGCAT TTACCAGAAA ACGAACCTGG TTCTTCTATT ATGCCAGGTA AAGTAAACCC TACACAAGTT GAAGCATTAA CAATGGTGTG CACTCAGGTG ATGGGTAATC ATGTGACTGT GACTATTGCT GGCTCAAATG GGCATCTTGA ACTTAACGTA TTTAAACCAG TGATAATATA CAATATCCTA CAATCTATAG AGCTATTATC TGATAGCGTA AATAGTTTTG TTACACACTG TGTTAAAGGA TTAGAGCCCA ATATAGCACG CATTAATACT TTACGTGATA AATCTCTAAT GCTTGTTACA GTTCTTAATC CACATATAGG CTATGATAAT GCTGCAAAAA TTGCTAAAGA AGCACATAAG TATGGGATCA CTTTAAAAGA AGCAGCTAAA AAACTCAATT TTTTATCAGA AGAAGAATTT GATAAAATAG TAGTACCTGA AAAAATGATT TCC
Details for RiprA.00047.a.A1.GE25412
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 3/6/2009 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKNYRIESD SFGEIQIEEK FYWGAQTQRS LNNFKISKQK MPKILIRALA ILKKCAAQVN YEFGDLEYKI ATSIDKAIDR ILAGEFEDNF PLVVWQTGSG TQTNMNMNEV IASIANEELT GKKGGKFPVH PNDHVNKGQS SNDSFPTAMH IATVLATKQQ LIPALNNLLT YLQDKSKDWD KIIKIGRTHL QDATPLTLKQ EFSGYITQIE YALERIEDAL KKVYLLAQGG TAVGTGINSK IGFDIKFAQK VAEFTQQPFK TAPNKFESLA AHDALVEFSG TLNTIAVSLM KIANDIRLLG SGPRCGLGEL HLPENEPGSS IMPGKVNPTQ VEALTMVCTQ VMGNHVTVTI AGSNGHLELN VFKPVIIYNI LQSIELLSDS VNSFVTHCVK GLEPNIARIN TLRDKSLMLV TVLNPHIGYD NAAKIAKEAH KYGITLKEAA KKLNFLSEEE FDKIVVPEKM IS
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaaaaat tacagaatcg aaagtgactc ttttggagaa attcaaatag aagaaaaatt ttattggggt gctcaaactc aaagatcttt aaacaatttt aaaataagca agcaaaaaat gccaaaaatt ttaattcgtg ctcttgctat tttaaaaaaa tgtgctgcac aggttaatta cgagtttggt gatttagaat ataaaatagc tacaagtata gataaagcaa ttgatagaat cctagcaggc gaatttgaag ataattttcc tttagtagtt tggcaaacag ggtcaggtac acaaacaaac atgaatatga acgaggttat tgcatctatt gctaatgaag aattaactgg taagaaaggt ggtaaattcc cagtacatcc taacgatcat gttaataaag ggcaatcatc caacgactct tttccaacag ctatgcatat agcaactgta cttgcaacta aacagcagct tataccagct ttgaataatt tacttacata tttgcaagac aagtcaaaag attgggataa aatcataaaa ataggacgca ctcacttgca ggatgcaact cctttaacac ttaaacagga attctcagga tatattacac aaatagagta tgctttagaa cgcatagaag atgcactaaa aaaagtttat ctacttgctc aaggcggaac agcagtcggt acaggtatta attcaaaaat aggctttgat ataaaatttg ctcaaaaagt tgcagagttt actcaacaac catttaaaac tgctcctaat aaatttgaaa gtttagcagc acatgatgca ctagtagaat tttctggaac tcttaatact atagcagtta gcttaatgaa aatagcaaat gatataagat tgctcggctc cggtccaaga tgcggtcttg gtgaactgca tttaccagaa aacgaacctg gttcttctat tatgccaggt aaagtaaacc ctacacaagt tgaagcatta acaatggtgt gcactcaggt gatgggtaat catgtgactg tgactattgc tggctcaaat gggcatcttg aacttaacgt atttaaacca gtgataatat acaatatcct acaatctata gagctattat ctgatagcgt aaatagtttt gttacacact gtgttaaagg attagagccc aatatagcac gcattaatac tttacgtgat aaatctctaa tgcttgttac agttcttaat ccacatatag gctatgataa tgctgcaaaa attgctaaag aagcacataa gtatgggatc actttaaaag aagcagctaa aaaactcaat tttttatcag aagaagaatt tgataaaata gtagtacctg aaaaaatgat ttcctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKNYRIESD SFGEIQIEEK FYWGAQTQRS LNNFKISKQK MPKILIRALA ILKKCAAQVN YEFGDLEYKI ATSIDKAIDR ILAGEFEDNF PLVVWQTGSG TQTNMNMNEV IASIANEELT GKKGGKFPVH PNDHVNKGQS SNDSFPTAMH IATVLATKQQ LIPALNNLLT YLQDKSKDWD KIIKIGRTHL QDATPLTLKQ EFSGYITQIE YALERIEDAL KKVYLLAQGG TAVGTGINSK IGFDIKFAQK VAEFTQQPFK TAPNKFESLA AHDALVEFSG TLNTIAVSLM KIANDIRLLG SGPRCGLGEL HLPENEPGSS IMPGKVNPTQ VEALTMVCTQ VMGNHVTVTI AGSNGHLELN VFKPVIIYNI LQSIELLSDS VNSFVTHCVK GLEPNIARIN TLRDKSLMLV TVLNPHIGYD NAAKIAKEAH KYGITLKEAA KKLNFLSEEE FDKIVVPEKM IS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaaattac agaatcgaaa gtgactcttt tggagaaatt caaatagaag aaaaatttta ttggggtgct caaactcaaa gatctttaaa caattttaaa ataagcaagc aaaaaatgcc aaaaatttta attcgtgctc ttgctatttt aaaaaaatgt gctgcacagg ttaattacga gtttggtgat ttagaatata aaatagctac aagtatagat aaagcaattg atagaatcct agcaggcgaa tttgaagata attttccttt agtagtttgg caaacagggt caggtacaca aacaaacatg aatatgaacg aggttattgc atctattgct aatgaagaat taactggtaa gaaaggtggt aaattcccag tacatcctaa cgatcatgtt aataaagggc aatcatccaa cgactctttt ccaacagcta tgcatatagc aactgtactt gcaactaaac agcagcttat accagctttg aataatttac ttacatattt gcaagacaag tcaaaagatt gggataaaat cataaaaata ggacgcactc acttgcagga tgcaactcct ttaacactta aacaggaatt ctcaggatat attacacaaa tagagtatgc tttagaacgc atagaagatg cactaaaaaa agtttatcta cttgctcaag gcggaacagc agtcggtaca ggtattaatt caaaaatagg ctttgatata aaatttgctc aaaaagttgc agagtttact caacaaccat ttaaaactgc tcctaataaa tttgaaagtt tagcagcaca tgatgcacta gtagaatttt ctggaactct taatactata gcagttagct taatgaaaat agcaaatgat ataagattgc tcggctccgg tccaagatgc ggtcttggtg aactgcattt accagaaaac gaacctggtt cttctattat gccaggtaaa gtaaacccta cacaagttga agcattaaca atggtgtgca ctcaggtgat gggtaatcat gtgactgtga ctattgctgg ctcaaatggg catcttgaac ttaacgtatt taaaccagtg ataatataca atatcctaca atctatagag ctattatctg atagcgtaaa tagttttgtt acacactgtg ttaaaggatt agagcccaat atagcacgca ttaatacttt acgtgataaa tctctaatgc ttgttacagt tcttaatcca catataggct atgataatgc tgcaaaaatt gctaaagaag cacataagta tgggatcact ttaaaagaag cagctaaaaa actcaatttt ttatcagaag aagaatttga taaaatagta gtacctgaaa aaatgatttc ctaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for RiprA.00047.a.A1.PS00079
| PURIFICATION DATe: | 9/12/2008 |
| CONCENTRATION: | 39.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKNYRIESD SFGEIQIEEK FYWGAQTQRS LNNFKISKQK MPKILIRALA ILKKCAAQVN YEFGDLEYKI ATSIDKAIDR ILAGEFEDNF PLVVWQTGSG TQTNMNMNEV IASIANEELT GKKGGKFPVH PNDHVNKGQS SNDSFPTAMH IATVLATKQQ LIPALNNLLT YLQDKSKDWD KIIKIGRTHL QDATPLTLKQ EFSGYITQIE YALERIEDAL KKVYLLAQGG TAVGTGINSK IGFDIKFAQK VAEFTQQPFK TAPNKFESLA AHDALVEFSG TLNTIAVSLM KIANDIRLLG SGPRCGLGEL HLPENEPGSS IMPGKVNPTQ VEALTMVCTQ VMGNHVTVTI AGSNGHLELN VFKPVIIYNI LQSIELLSDS VNSFVTHCVK GLEPNIARIN TLRDKSLMLV TVLNPHIGYD NAAKIAKEAH KYGITLKEAA KKLNFLSEEE FDKIVVPEKM IS
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaaaaat tacagaatcg aaagtgactc ttttggagaa attcaaatag aagaaaaatt ttattggggt gctcaaactc aaagatcttt aaacaatttt aaaataagca agcaaaaaat gccaaaaatt ttaattcgtg ctcttgctat tttaaaaaaa tgtgctgcac aggttaatta cgagtttggt gatttagaat ataaaatagc tacaagtata gataaagcaa ttgatagaat cctagcaggc gaatttgaag ataattttcc tttagtagtt tggcaaacag ggtcaggtac acaaacaaac atgaatatga acgaggttat tgcatctatt gctaatgaag aattaactgg taagaaaggt ggtaaattcc cagtacatcc taacgatcat gttaataaag ggcaatcatc caacgactct tttccaacag ctatgcatat agcaactgta cttgcaacta aacagcagct tataccagct ttgaataatt tacttacata tttgcaagac aagtcaaaag attgggataa aatcataaaa ataggacgca ctcacttgca ggatgcaact cctttaacac ttaaacagga attctcagga tatattacac aaatagagta tgctttagaa cgcatagaag atgcactaaa aaaagtttat ctacttgctc aaggcggaac agcagtcggt acaggtatta attcaaaaat aggctttgat ataaaatttg ctcaaaaagt tgcagagttt actcaacaac catttaaaac tgctcctaat aaatttgaaa gtttagcagc acatgatgca ctagtagaat tttctggaac tcttaatact atagcagtta gcttaatgaa aatagcaaat gatataagat tgctcggctc cggtccaaga tgcggtcttg gtgaactgca tttaccagaa aacgaacctg gttcttctat tatgccaggt aaagtaaacc ctacacaagt tgaagcatta acaatggtgt gcactcaggt gatgggtaat catgtgactg tgactattgc tggctcaaat gggcatcttg aacttaacgt atttaaacca gtgataatat acaatatcct acaatctata gagctattat ctgatagcgt aaatagtttt gttacacact gtgttaaagg attagagccc aatatagcac gcattaatac tttacgtgat aaatctctaa tgcttgttac agttcttaat ccacatatag gctatgataa tgctgcaaaa attgctaaag aagcacataa gtatgggatc actttaaaag aagcagctaa aaaactcaat tttttatcag aagaagaatt tgataaaata gtagtacctg aaaaaatgat ttcctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKNYRIESD SFGEIQIEEK FYWGAQTQRS LNNFKISKQK MPKILIRALA ILKKCAAQVN YEFGDLEYKI ATSIDKAIDR ILAGEFEDNF PLVVWQTGSG TQTNMNMNEV IASIANEELT GKKGGKFPVH PNDHVNKGQS SNDSFPTAMH IATVLATKQQ LIPALNNLLT YLQDKSKDWD KIIKIGRTHL QDATPLTLKQ EFSGYITQIE YALERIEDAL KKVYLLAQGG TAVGTGINSK IGFDIKFAQK VAEFTQQPFK TAPNKFESLA AHDALVEFSG TLNTIAVSLM KIANDIRLLG SGPRCGLGEL HLPENEPGSS IMPGKVNPTQ VEALTMVCTQ VMGNHVTVTI AGSNGHLELN VFKPVIIYNI LQSIELLSDS VNSFVTHCVK GLEPNIARIN TLRDKSLMLV TVLNPHIGYD NAAKIAKEAH KYGITLKEAA KKLNFLSEEE FDKIVVPEKM IS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAAATTAC AGAATCGAAA GTGACTCTTT TGGAGAAATT CAAATAGAAG AAAAATTTTA TTGGGGTGCT CAAACTCAAA GATCTTTAAA CAATTTTAAA ATAAGCAAGC AAAAAATGCC AAAAATTTTA ATTCGTGCTC TTGCTATTTT AAAAAAATGT GCTGCACAGG TTAATTACGA GTTTGGTGAT TTAGAATATA AAATAGCTAC AAGTATAGAT AAAGCAATTG ATAGAATCCT AGCAGGCGAA TTTGAAGATA ATTTTCCTTT AGTAGTTTGG CAAACAGGGT CAGGTACACA AACAAACATG AATATGAACG AGGTTATTGC ATCTATTGCT AATGAAGAAT TAACTGGTAA GAAAGGTGGT AAATTCCCAG TACATCCTAA CGATCATGTT AATAAAGGGC AATCATCCAA CGACTCTTTT CCAACAGCTA TGCATATAGC AACTGTACTT GCAACTAAAC AGCAGCTTAT ACCAGCTTTG AATAATTTAC TTACATATTT GCAAGACAAG TCAAAAGATT GGGATAAAAT CATAAAAATA GGACGCACTC ACTTGCAGGA TGCAACTCCT TTAACACTTA AACAGGAATT CTCAGGATAT ATTACACAAA TAGAGTATGC TTTAGAACGC ATAGAAGATG CACTAAAAAA AGTTTATCTA CTTGCTCAAG GCGGAACAGC AGTCGGTACA GGTATTAATT CAAAAATAGG CTTTGATATA AAATTTGCTC AAAAAGTTGC AGAGTTTACT CAACAACCAT TTAAAACTGC TCCTAATAAA TTTGAAAGTT TAGCAGCACA TGATGCACTA GTAGAATTTT CTGGAACTCT TAATACTATA GCAGTTAGCT TAATGAAAAT AGCAAATGAT ATAAGATTGC TCGGCTCCGG TCCAAGATGC GGTCTTGGTG AACTGCATTT ACCAGAAAAC GAACCTGGTT CTTCTATTAT GCCAGGTAAA GTAAACCCTA CACAAGTTGA AGCATTAACA ATGGTGTGCA CTCAGGTGAT GGGTAATCAT GTGACTGTGA CTATTGCTGG CTCAAATGGG CATCTTGAAC TTAACGTATT TAAACCAGTG ATAATATACA ATATCCTACA ATCTATAGAG CTATTATCTG ATAGCGTAAA TAGTTTTGTT ACACACTGTG TTAAAGGATT AGAGCCCAAT ATAGCACGCA TTAATACTTT ACGTGATAAA TCTCTAATGC TTGTTACAGT TCTTAATCCA CATATAGGCT ATGATAATGC TGCAAAAATT GCTAAAGAAG CACATAAGTA TGGGATCACT TTAAAAGAAG CAGCTAAAAA ACTCAATTTT TTATCAGAAG AAGAATTTGA TAAAATAGTA GTACCTGAAA AAATGATTTC CTAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for RiprA.00047.a.A1.PS00090
| PURIFICATION DATe: | 10/10/2008 |
| CONCENTRATION: | 59.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKNYRIESD SFGEIQIEEK FYWGAQTQRS LNNFKISKQK MPKILIRALA ILKKCAAQVN YEFGDLEYKI ATSIDKAIDR ILAGEFEDNF PLVVWQTGSG TQTNMNMNEV IASIANEELT GKKGGKFPVH PNDHVNKGQS SNDSFPTAMH IATVLATKQQ LIPALNNLLT YLQDKSKDWD KIIKIGRTHL QDATPLTLKQ EFSGYITQIE YALERIEDAL KKVYLLAQGG TAVGTGINSK IGFDIKFAQK VAEFTQQPFK TAPNKFESLA AHDALVEFSG TLNTIAVSLM KIANDIRLLG SGPRCGLGEL HLPENEPGSS IMPGKVNPTQ VEALTMVCTQ VMGNHVTVTI AGSNGHLELN VFKPVIIYNI LQSIELLSDS VNSFVTHCVK GLEPNIARIN TLRDKSLMLV TVLNPHIGYD NAAKIAKEAH KYGITLKEAA KKLNFLSEEE FDKIVVPEKM IS
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaaaaat tacagaatcg aaagtgactc ttttggagaa attcaaatag aagaaaaatt ttattggggt gctcaaactc aaagatcttt aaacaatttt aaaataagca agcaaaaaat gccaaaaatt ttaattcgtg ctcttgctat tttaaaaaaa tgtgctgcac aggttaatta cgagtttggt gatttagaat ataaaatagc tacaagtata gataaagcaa ttgatagaat cctagcaggc gaatttgaag ataattttcc tttagtagtt tggcaaacag ggtcaggtac acaaacaaac atgaatatga acgaggttat tgcatctatt gctaatgaag aattaactgg taagaaaggt ggtaaattcc cagtacatcc taacgatcat gttaataaag ggcaatcatc caacgactct tttccaacag ctatgcatat agcaactgta cttgcaacta aacagcagct tataccagct ttgaataatt tacttacata tttgcaagac aagtcaaaag attgggataa aatcataaaa ataggacgca ctcacttgca ggatgcaact cctttaacac ttaaacagga attctcagga tatattacac aaatagagta tgctttagaa cgcatagaag atgcactaaa aaaagtttat ctacttgctc aaggcggaac agcagtcggt acaggtatta attcaaaaat aggctttgat ataaaatttg ctcaaaaagt tgcagagttt actcaacaac catttaaaac tgctcctaat aaatttgaaa gtttagcagc acatgatgca ctagtagaat tttctggaac tcttaatact atagcagtta gcttaatgaa aatagcaaat gatataagat tgctcggctc cggtccaaga tgcggtcttg gtgaactgca tttaccagaa aacgaacctg gttcttctat tatgccaggt aaagtaaacc ctacacaagt tgaagcatta acaatggtgt gcactcaggt gatgggtaat catgtgactg tgactattgc tggctcaaat gggcatcttg aacttaacgt atttaaacca gtgataatat acaatatcct acaatctata gagctattat ctgatagcgt aaatagtttt gttacacact gtgttaaagg attagagccc aatatagcac gcattaatac tttacgtgat aaatctctaa tgcttgttac agttcttaat ccacatatag gctatgataa tgctgcaaaa attgctaaag aagcacataa gtatgggatc actttaaaag aagcagctaa aaaactcaat tttttatcag aagaagaatt tgataaaata gtagtacctg aaaaaatgat ttcctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKNYRIESD SFGEIQIEEK FYWGAQTQRS LNNFKISKQK MPKILIRALA ILKKCAAQVN YEFGDLEYKI ATSIDKAIDR ILAGEFEDNF PLVVWQTGSG TQTNMNMNEV IASIANEELT GKKGGKFPVH PNDHVNKGQS SNDSFPTAMH IATVLATKQQ LIPALNNLLT YLQDKSKDWD KIIKIGRTHL QDATPLTLKQ EFSGYITQIE YALERIEDAL KKVYLLAQGG TAVGTGINSK IGFDIKFAQK VAEFTQQPFK TAPNKFESLA AHDALVEFSG TLNTIAVSLM KIANDIRLLG SGPRCGLGEL HLPENEPGSS IMPGKVNPTQ VEALTMVCTQ VMGNHVTVTI AGSNGHLELN VFKPVIIYNI LQSIELLSDS VNSFVTHCVK GLEPNIARIN TLRDKSLMLV TVLNPHIGYD NAAKIAKEAH KYGITLKEAA KKLNFLSEEE FDKIVVPEKM IS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAAATTAC AGAATCGAAA GTGACTCTTT TGGAGAAATT CAAATAGAAG AAAAATTTTA TTGGGGTGCT CAAACTCAAA GATCTTTAAA CAATTTTAAA ATAAGCAAGC AAAAAATGCC AAAAATTTTA ATTCGTGCTC TTGCTATTTT AAAAAAATGT GCTGCACAGG TTAATTACGA GTTTGGTGAT TTAGAATATA AAATAGCTAC AAGTATAGAT AAAGCAATTG ATAGAATCCT AGCAGGCGAA TTTGAAGATA ATTTTCCTTT AGTAGTTTGG CAAACAGGGT CAGGTACACA AACAAACATG AATATGAACG AGGTTATTGC ATCTATTGCT AATGAAGAAT TAACTGGTAA GAAAGGTGGT AAATTCCCAG TACATCCTAA CGATCATGTT AATAAAGGGC AATCATCCAA CGACTCTTTT CCAACAGCTA TGCATATAGC AACTGTACTT GCAACTAAAC AGCAGCTTAT ACCAGCTTTG AATAATTTAC TTACATATTT GCAAGACAAG TCAAAAGATT GGGATAAAAT CATAAAAATA GGACGCACTC ACTTGCAGGA TGCAACTCCT TTAACACTTA AACAGGAATT CTCAGGATAT ATTACACAAA TAGAGTATGC TTTAGAACGC ATAGAAGATG CACTAAAAAA AGTTTATCTA CTTGCTCAAG GCGGAACAGC AGTCGGTACA GGTATTAATT CAAAAATAGG CTTTGATATA AAATTTGCTC AAAAAGTTGC AGAGTTTACT CAACAACCAT TTAAAACTGC TCCTAATAAA TTTGAAAGTT TAGCAGCACA TGATGCACTA GTAGAATTTT CTGGAACTCT TAATACTATA GCAGTTAGCT TAATGAAAAT AGCAAATGAT ATAAGATTGC TCGGCTCCGG TCCAAGATGC GGTCTTGGTG AACTGCATTT ACCAGAAAAC GAACCTGGTT CTTCTATTAT GCCAGGTAAA GTAAACCCTA CACAAGTTGA AGCATTAACA ATGGTGTGCA CTCAGGTGAT GGGTAATCAT GTGACTGTGA CTATTGCTGG CTCAAATGGG CATCTTGAAC TTAACGTATT TAAACCAGTG ATAATATACA ATATCCTACA ATCTATAGAG CTATTATCTG ATAGCGTAAA TAGTTTTGTT ACACACTGTG TTAAAGGATT AGAGCCCAAT ATAGCACGCA TTAATACTTT ACGTGATAAA TCTCTAATGC TTGTTACAGT TCTTAATCCA CATATAGGCT ATGATAATGC TGCAAAAATT GCTAAAGAAG CACATAAGTA TGGGATCACT TTAAAAGAAG CAGCTAAAAA ACTCAATTTT TTATCAGAAG AAGAATTTGA TAAAATAGTA GTACCTGAAA AAATGATTTC CTAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA