CENTER ID: | RiprA.00045.a |
ORGANISM: | Rickettsia prowazekii str. Madrid E |
ASSOCIATED DISEASE: | Endemic typhus |
CURRENT STATUS: | purified |
COMMUNITY REQUEST: | False |
NIH RISK GROUP: | 3 |
SELECT AGENT: | True |
NIH PRIORITY pathogens category: |
IIIB/C |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
RiprA.00045.a.A1.GE25411 | Full length( RiprA.00045.a ) | 1 | 229 | ||
RiprA.00045.a.A2.GE28221 | Created from Decode construct for feature RiprA.00045.a | 1 | 174 | ||
RiprA.00045.a.A3.GE28222 | Created from Decode construct for feature RiprA.00045.a | 1 | 184 | ||
RiprA.00045.a.A5.GE28224 | Created from Decode construct for feature RiprA.00045.a | 2 | 178 | ||
RiprA.00045.a.A6.GE28225 | Created from Decode construct for feature RiprA.00045.a | 2 | 184 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
RiprA.00045.a.A1.PS00133 | Full length( RiprA.00045.a ) | 1 | 229 | ||
RiprA.00045.a.A1.PS00134 | Full length( RiprA.00045.a ) | 1 | 229 | ||
RiprA.00045.a.A1.PW25667 | Full length( RiprA.00045.a ) | 1 | 229 | ||
RiprA.00045.a.A2.PW28142 | Created from Decode construct for feature RiprA.00045.a | 1 | 174 | ||
RiprA.00045.a.A3.PW28143 | Created from Decode construct for feature RiprA.00045.a | 1 | 184 | ||
RiprA.00045.a.A3.PW31450 | Created from Decode construct for feature RiprA.00045.a | 1 | 184 |
RESOURCE | REFERENCE ID |
---|---|
OrthoMCL: | OG5_133735 |
PATRIC ID: | fig|272947.5.peg.766 |
RefSeq: | NP_221085 |
UniProt: | Q9ZCJ9 |
HARVESTED ON: | 6/30/2008 |
SEQUENCED ON: | 3/27/2012 |
EXPECTED MW: | 28kDa |
OBSERVED MW: | 28kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | High Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 98 |
HARVESTED ON: | 1/20/2010 |
SEQUENCED ON: | 6/16/2010 |
EXPECTED MW: | 22kDa |
OBSERVED MW: | 22kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Low Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass with sequence variation |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 1/20/2010 |
SEQUENCED ON: | 6/16/2010 |
EXPECTED MW: | 23kDa |
OBSERVED MW: | 23kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass with sequence variation |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 2/2/2010 |
SEQUENCED ON: | 2/22/2010 |
EXPECTED MW: | 22kDa |
OBSERVED MW: | 20kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Few (1-10) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass with sequence variation |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 98 |
HARVESTED ON: | 2/2/2010 |
SEQUENCED ON: | 2/22/2010 |
EXPECTED MW: | 23kDa |
OBSERVED MW: | 22kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Few (1-10) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | High Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
PURIFICATION DATe: | 10/17/2008 |
CONCENTRATION: | 30.28mg/ml |
OBSERVED MW: | data unavailable |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 6 |
VIAL VOLUME: | data unavailable |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 98 |
PURIFICATION DATe: | 10/17/2008 |
CONCENTRATION: | 15.95mg/ml |
OBSERVED MW: | data unavailable |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 7 |
VIAL VOLUME: | data unavailable |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 98 |
PURIFICATION DATe: | 9/15/2008 |
CONCENTRATION: | 19.18mg/ml |
OBSERVED MW: | data unavailable |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 3 |
VIAL VOLUME: | 150µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 98 |
PURIFICATION DATe: | 2/22/2010 |
CONCENTRATION: | 27.16mg/ml |
OBSERVED MW: | data unavailable |
EXPRESSION LEVEL: | Moderate Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 10 |
VIAL VOLUME: | 100µl |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
PURIFICATION DATe: | 2/22/2010 |
CONCENTRATION: | 25.51mg/ml |
OBSERVED MW: | data unavailable |
EXPRESSION LEVEL: | Moderate Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 14 |
VIAL VOLUME: | 100µl |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
PURIFICATION DATe: | 1/10/2011 |
CONCENTRATION: | 31.62mg/ml |
OBSERVED MW: | data unavailable |
EXPRESSION LEVEL: | Moderate Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 11 |
VIAL VOLUME: | 200µl |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |