RiprA.00039.a
methionine aminopeptidase
| CENTER ID: | RiprA.00039.a |
| ORGANISM: | Rickettsia prowazekii str. Madrid E |
| ASSOCIATED DISEASE: | Endemic typhus |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiprA.00039.a.A2.GE28216 | Created from Decode construct for feature RiprA.00039.a | 1 | 247 | ||
| RiprA.00039.a.A3.GE28217 | Created from Decode construct for feature RiprA.00039.a | 1 | 249 | ||
| RiprA.00039.a.A4.GE28218 | Created from Decode construct for feature RiprA.00039.a | 3 | 259 | ||
| RiprA.00039.a.A6.GE28220 | Created from Decode construct for feature RiprA.00039.a | 3 | 249 | ||
| RiprA.00039.a.B1.GE26993 | Full length( RiprA.00039.a ) | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiprA.00039.a.B1.PS00343 | Full length( RiprA.00039.a ) | 1 | 259 |
Structures
3MX6
DEPOSITED: 5/6/2010
DETERMINATION: XRay
CLONE: RiprA.00039.a.A4.GE28218
PROTEIN: RiprA.00039.a.A4.PW28139
3MR1
DEPOSITED: 4/28/2010
DETERMINATION: XRay
CLONE: RiprA.00039.a.A6.GE28220
PROTEIN: RiprA.00039.a.A6.PW28141
Publications by SSGCID
Rickettsia prowazekii Methionine Aminopeptidase as a Promising Target for the Development of Antibacterial Agents
Abendroth JA, Edwards TE, Hagen TJ, Helgren TR, Horn JR, Myler PJ, Sankaran B, Staker BL, Wangtrakuldee P, Chen C, Audia J, Housley NA
Bioorg. Med. Chem. - 2016
volume 16, issue , pages S0968-0896
PMID: 28089350; PMCID: PMC5319851
The Identification of Inhibitory Compounds of Rickettsia prowazekii Methionine Aminopeptidase for Antibacterial Applications
Abendroth JA, Edwards TE, Hagen TJ, Helgren TR, Horn JR, Myler PJ, Staker BL, Chen C, Seven E
Bioorg. Med. Chem. Lett. - 2018
volume 28, pages 1376-1380
PMID: 29551481; PMCID: PMC5908248
PDB:
3MX6
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|272947.5.peg.860 |
| RefSeq: | NP_221173 |
| UniProt: | Q9ZCD3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIKIHTEKD FIKMRAAGKL AAETLDFITD HVKPNVTTNS LNDLCHNFIT SHNAIPAPLN YKGFPKSICT SINHVVCHGI PNDKPLKNGD IVNIDVTVIL DGWYGDTSRM YYVGDVAIKP KRLIQVTYDA MMKGIEVVRP GAKLGDIGYA IQSYAEKHNY SVVRDYTGHG IGRVFHDKPS ILNYGRNGTG LTLKEGMFFT VEPMINAGNY DTILSKLDGW TVTTRDKSLS AQFEHTIGVT KDGFEIFTLS PKKLDYPPY
NT Sequence
ATGACTATAA AAATCCACAC TGAAAAAGAT TTTATAAAGA TGCGGGCAGC AGGAAAATTA GCTGCAGAGA CACTCGATTT TATTACTGAT CACGTCAAAC CTAATGTCAC TACTAATAGC TTGAATGACC TTTGTCATAA TTTTATTACT TCTCATAATG CAATTCCTGC ACCGTTAAAT TACAAAGGCT TTCCAAAATC GATTTGTACT TCTATAAATC ATGTAGTTTG TCACGGAATT CCAAATGATA AACCATTAAA AAATGGTGAT ATAGTAAATA TTGATGTTAC GGTAATTTTA GATGGCTGGT ATGGTGATAC TAGTCGTATG TATTACGTCG GTGACGTAGC AATTAAACCA AAGCGTCTTA TTCAAGTAAC TTATGATGCA ATGATGAAAG GAATTGAAGT AGTGAGACCA GGTGCTAAAC TCGGCGATAT CGGGTATGCA ATTCAAAGTT ATGCCGAGAA ACATAATTAT TCTGTGGTTA GAGATTATAC GGGTCACGGT ATTGGAAGAG TTTTTCATGA TAAACCTTCA ATATTAAATT ACGGCCGCAA CGGCACAGGT CTAACGTTAA AAGAAGGAAT GTTTTTTACT GTTGAACCTA TGATAAATGC AGGTAATTAT GACACTATAT TAAGTAAATT AGATGGATGG ACAGTGACCA CGCGTGATAA ATCATTATCG GCACAATTTG AGCATACTAT AGGCGTGACT AAAGATGGCT TTGAAATATT TACATTATCA CCTAAAAAAC TTGATTATCC TCCATAC
Details for RiprA.00039.a.A2.GE28216
| HARVESTED ON: | 1/20/2010 |
| SEQUENCED ON: | 6/16/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTIKIHTEK DFIKMRAAGK LAAETLDFIT DHVKPNVTTN SLNDLCHNFI TSHNAIPAPL NYKGFPKSIC TSINHVVCHG IPNDKPLKNG DIVNIDVTVI LDGWYGDTSR MYYVGDVAIK PKRLIQVTYD AMMKGIEVVR PGAKLGDIGY AIQSYAEKHN YSVVRDYTGH GIGRVFHDKP SILNYGRNGT GLTLKEGMFF TVEPMINAGN YDTILSKLDG WTVTTRDKSL SAQFEHTIGV TKDGFEIF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactataaaa atccacactg aaaaagattt tataaagatg cgggcagcag gaaaattagc tgcagagaca ctcgatttta ttactgatca cgtcaaacct aatgtcacta ctaatagctt gaatgacctt tgtcataatt ttattacttc tcataatgca attcctgcac cgttaaatta caaaggcttt ccaaaatcga tttgtacttc tataaatcat gtagtttgtc acggaattcc aaatgataaa ccattaaaaa atggtgatat agtaaatatt gatgttacgg taattttaga tggctggtat ggtgatacta gtcgtatgta ttacgtcggt gacgtagcaa ttaaaccaaa gcgtcttatt caagtaactt atgatgcaat gatgaaagga attgaagtag tgagaccagg tgctaaactc ggcgatatcg ggtatgcaat tcaaagttat gccgagaaac ataattattc tgtggttaga gattatacgg gtcacggtat tggaagagtt tttcatgata aaccttcaat attaaattac ggccgcaacg gcacaggtct aacgttaaaa gaaggaatgt tttttactgt tgaacctatg ataaatgcag gtaattatga cactatatta agtaaattag atggatggac agtgaccacg cgtgataaat cattatcggc acaatttgag catactatag gcgtgactaa agatggcttt gaaatattta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for RiprA.00039.a.A3.GE28217
| HARVESTED ON: | 1/20/2010 |
| SEQUENCED ON: | 6/16/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTIKIHTEK DFIKMRAAGK LAAETLDFIT DHVKPNVTTN SLNDLCHNFI TSHNAIPAPL NYKGFPKSIC TSINHVVCHG IPNDKPLKNG DIVNIDVTVI LDGWYGDTSR MYYVGDVAIK PKRLIQVTYD AMMKGIEVVR PGAKLGDIGY AIQSYAEKHN YSVVRDYTGH GIGRVFHDKP SILNYGRNGT GLTLKEGMFF TVEPMINAGN YDTILSKLDG WTVTTRDKSL SAQFEHTIGV TKDGFEIFTL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacta taaaaatcca cactgaaaaa gattttataa agatgcgggc agcaggaaaa ttagctgcag agacactcga ttttattact gatcacgtca aacctaatgt cactactaat agcttgaatg acctttgtca taattttatt acttctcata atgcaattcc tgcaccgtta aattacaaag gctttccaaa atcgatttgt acttctataa atcatgtagt ttgtcacgga attccaaatg ataaaccatt aaaaaatggt gatatagtaa atattgatgt tacggtaatt ttagatggct ggtatggtga tactagtcgt atgtattacg tcggtgacgt agcaattaaa ccaaagcgtc ttattcaagt aacttatgat gcaatgatga aaggaattga agtagtgaga ccaggtgcta aactcggcga catcgggtat gcaattcaaa gttatgccga gaaacataat tattctgtgg ttagagatta tacgggtcac ggtattggaa gagtttttca tgataaacct tcaatattaa attacggccg caacggcaca ggtctaacgt taaaagaagg aatgtttttt actgttgaac ctatgataaa tgcaggtaat tatgacacta tattaagtaa attagatgga tggacagtga ccacgcgtga taaatcatta tcggcacaat ttgagcatac tataggcgtg actaaagatg gctttgaaat atttacatta taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTIKIHTEK DFIKMRAAGK LAAETLDFIT DHVKPNVTTN SLNDLCHNFI TSHNAIPAPL NYKGFPKSIC TSINHVVCHG IPNDKPLKNG DIVNIDVTVI LDGWYGDTSR MYYVGDVAIK PKRLIQVTYD AMMKGIEVVR PGAKLGDIGY AIQSYAEKHN YSVVRDYTGH GIGRVFHDKP SILNYGRNGT GLTLKEGMFF TVEPMINAGN YDTILSKLDG WTVTTRDKSL SAQFEHTIGV TKDGFEIFTL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactataaaa atccacactg aaaaagattt tataaagatg cgggcagcag gaaaattagc tgcagagaca ctcgatttta ttactgatca cgtcaaacct aatgtcacta ctaatagctt gaatgacctt tgtcataatt ttattacttc tcataatgca attcctgcac cgttaaatta caaaggcttt ccaaaatcga tttgtacttc tataaatcat gtagtttgtc acggaattcc aaatgataaa ccattaaaaa atggtgatat agtaaatatt gatgttacgg taattttaga tggctggtat ggtgatacta gtcgtatgta ttacgtcggt gacgtagcaa ttaaaccaaa gcgtcttatt caagtaactt atgatgcaat gatgaaagga attgaagtag tgagaccagg tgctaaactc ggcgatatcg ggtatgcaat tcaaagttat gccgagaaac ataattattc tgtggttaga gattatacgg gtcacggtat tggaagagtt tttcatgata aaccttcaat attaaattac ggccgcaacg gcacaggtct aacgttaaaa gaaggaatgt tttttactgt tgaacctatg ataaatgcag gtaattatga cactatatta agtaaattag atggatggac agtgaccacg cgtgataaat cattatcggc acaatttgag catactatag gcgtgactaa agatggcttt gaaatattta cattaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for RiprA.00039.a.A4.GE28218
| HARVESTED ON: | 2/2/2010 |
| SEQUENCED ON: | 12/18/2014 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIKIHTEKD FIKMRAAGKL AAETLDFITD HVKPNVTTNS LNDLCHNFIT SHNAIPAPLN YKGFPKSICT SINHVVCHGI PNDKPLKNGD IVNIDVTVIL DGWYGDTSRM YYVGDVAIKP KRLIQVTYDA MMKGIEVVRP GAKLGDIGYA IQSYAEKHNY SVVRDYTGHG IGRVFHDKPS ILNYGRNGTG LTLKEGMFFT VEPMINAGNY DTILSKLDGW TVTTRDKSLS AQFEHTIGVT KDGFEIFTLS PKKLDYPPY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gataaaaatc cacactgaaa aagattttat aaagatgcgg gcagcaggaa aattagctgc agagacactc gattttatta ctgatcacgt caaacctaat gtcactacta atagcttgaa tgacctttgt cataatttta ttacttctca taatgcaatt cctgcaccgt taaattacaa aggctttcca aaatcgattt gtacttctat aaatcatgta gtttgtcacg gaattccaaa tgataaacca ttaaaaaatg gtgatatagt aaatattgat gttacggtaa ttttagatgg ctggtatggt gatactagtc gtatgtatta cgtcggtgac gtagcaatta aaccaaagcg tcttattcaa gtaacttatg atgcaatgat gaaaggaatt gaagtagtga gaccaggtgc taaactcggc gatatcgggt atgcaattca aagttatgcc gagaaacata attattctgt ggttagagat tatacgggtc acggtattgg aagagttttt catgataaac cttcaatatt aaattacggc cgcaacggca caggtctaac gttaaaagaa ggaatgtttt ttactgttga acctatgata aatgcaggta attatgacac tatattaagt aaattagatg gatggacagt gaccacgcgt gataaatcat tatcggcaca atttgagcat actataggcg tgactaaaga tggctttgaa atatttacat tatcacctaa aaaacttgat tatcctccat acaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for RiprA.00039.a.A6.GE28220
| HARVESTED ON: | 2/2/2010 |
| SEQUENCED ON: | 2/22/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIKIHTEKD FIKMRAAGKL AAETLDFITD HVKPNVTTNS LNDLCHNFIT SHNAIPAPLN YKGFPKSICT SINHVVCHGI PNDKPLKNGD IVNIDVTVIL DGWYGDTSRM YYVGDVAIKP KRLIQVTYDA MMKGIEVVRP GAKLGDIGYA IQSYAEKHNY SVVRDYTGHG IGRVFHDKPS ILNYGRNGTG LTLKEGMFFT VEPMINAGNY DTILSKLDGW TVTTRDKSLS AQFEHTIGVT KDGFEIFTL
Validated NT Sequence
ttattataat gtaaatattt caaagccatc tttagtcacg cctatagtat gctcaaattg tgccgataat gatttatcac gcgtggtcac tgtccatcca tctaatttac ttaatatagt gtcataatta cctgcattta tcataggttc aacagtaaaa aacattcctt cttttaacgt tagacctgtg ccgttgcggc cgtaatttaa tattgaaggt ttatcatgaa aaactcttcc aataccgtga cccgtataat ctctaaccac agaataatta tgtttctcgg cataactttg aattgcatac ccgatatcgc cgagtttagc acctggtctc actacttcaa ttcctttcat cattgcatca taagttactt gaataagacg ctttggttta attgctacgt caccgacgta atacatacga ctagtatcac cataccagcc atctaaaatt accgtaacat caatatttac tatatcacca ttttttaatg gtttatcatt tggaattccg tgacaaacta catgatttat agaagtacaa atcgattttg gaaagccttt gtaatttaac ggtgcaggaa ttgcattatg agaagtaata aaattatgac aaaggtcatt caagctatta gtagtgacat taggtttgac gtgatcagta ataaaatcga gtgtctctgc agctaatttt cctgctgccc gcatctttat aaaatctttt tcagtgtgga tttttatcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIKIHTEKD FIKMRAAGKL AAETLDFITD HVKPNVTTNS LNDLCHNFIT SHNAIPAPLN YKGFPKSICT SINHVVCHGI PNDKPLKNGD IVNIDVTVIL DGWYGDTSRM YYVGDVAIKP KRLIQVTYDA MMKGIEVVRP GAKLGDIGYA IQSYAEKHNY SVVRDYTGHG IGRVFHDKPS ILNYGRNGTG LTLKEGMFFT VEPMINAGNY DTILSKLDGW TVTTRDKSLS AQFEHTIGVT KDGFEIFTL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gataaaaatc cacactgaaa aagattttat aaagatgcgg gcagcaggaa aattagctgc agagacactc gattttatta ctgatcacgt caaacctaat gtcactacta atagcttgaa tgacctttgt cataatttta ttacttctca taatgcaatt cctgcaccgt taaattacaa aggctttcca aaatcgattt gtacttctat aaatcatgta gtttgtcacg gaattccaaa tgataaacca ttaaaaaatg gtgatatagt aaatattgat gttacggtaa ttttagatgg ctggtatggt gatactagtc gtatgtatta cgtcggtgac gtagcaatta aaccaaagcg tcttattcaa gtaacttatg atgcaatgat gaaaggaatt gaagtagtga gaccaggtgc taaactcggc gatatcgggt atgcaattca aagttatgcc gagaaacata attattctgt ggttagagat tatacgggtc acggtattgg aagagttttt catgataaac cttcaatatt aaattacggc cgcaacggca caggtctaac gttaaaagaa ggaatgtttt ttactgttga acctatgata aatgcaggta attatgacac tatattaagt aaattagatg gatggacagt gaccacgcgt gataaatcat tatcggcaca atttgagcat actataggcg tgactaaaga tggctttgaa atatttacat taaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for RiprA.00039.a.B1.GE26993
| HARVESTED ON: | 6/18/2009 |
| SEQUENCED ON: | 7/15/2009 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT IKIHTEKDFI KMRAAGKLAA ETLDFITDHV KPNVTTNSLN DLCHNFITSH NAIPAPLNYK GFPKSICTSI NHVVCHGIPN DKPLKNGDIV NIDVTVILDG WYGDTSRMYY VGDVAIKPKR LIQVTYDAMM KGIEVVRPGA KLGDIGYAIQ SYAEKHNYSV VRDYTGHGIG RVFHDKPSVL NYGRNGTGLT LKEGMFFTVE PMINAGNYDT ILSRLDGWTV TTRDKSLSAQ FEHTIGVTKD GFEIFTLSPK KLDYPPY
Validated NT Sequence
atcttactca cttagtatgg aggataatca agttttttag gtgataatgt aaatatttca aagccatctt tagtcacgcc tatagtatgc tcaaattgtg ccgataatga tttatcacgc gtggtcactg tccatccatc taatctactt aatatagtgt cataattacc tgcatttatc ataggttcaa cagtaaaaaa cattccttct tttaacgtta gacctgtgcc gttgcggccg taatttaata ctgaaggttt atcatgaaaa actcttccaa taccgtgacc cgtataatct ctaaccacag aataattatg tttctcggca taactttgaa ttgcataccc gatatcgccg agtttagcac ctggtctcac tacttcaatt cctttcatca ttgcatcata agttacttga ataagacgct ttggtttaat tgctacgtca ccgacgtaat acatacgact agtatcacca taccagccat ctaaaattac cgtaacatca atatttacta tatcaccatt ttttaatggt ttatcatttg gaattccgtg acaaactaca tgatttatag aagtacaaat cgattttgga aagcctttgt aatttaacgg tgcaggaatt gcattatgag aagtaataaa attatgacaa aggtcattca agctattagt agtgacatta ggtttgacgt gatcagtaat aaaatcgagt gtctctgcag ctaattttcc tgctgcccgc atctttataa aatctttttc agtgtggatt tttatagtca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMT IKIHTEKDFI KMRAAGKLAA ETLDFITDHV KPNVTTNSLN DLCHNFITSH NAIPAPLNYK GFPKSICTSI NHVVCHGIPN DKPLKNGDIV NIDVTVILDG WYGDTSRMYY VGDVAIKPKR LIQVTYDAMM KGIEVVRPGA KLGDIGYAIQ SYAEKHNYSV VRDYTGHGIG RVFHDKPSIL NYGRNGTGLT LKEGMFFTVE PMINAGNYDT ILSKLDGWTV TTRDKSLSAQ FEHTIGVTKD GFEIFTLSPK KLDYPPY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta taaaaatcca cactgaaaaa gattttataa agatgcgggc agcaggaaaa ttagctgcag agacactcga ttttattact gatcacgtca aacctaatgt cactactaat agcttgaatg acctttgtca taattttatt acttctcata atgcaattcc tgcaccgtta aattacaaag gctttccaaa atcgatttgt acttctataa atcatgtagt ttgtcacgga attccaaatg ataaaccatt aaaaaatggt gatatagtaa atattgatgt tacggtaatt ttagatggct ggtatggtga tactagtcgt atgtattacg tcggtgacgt agcaattaaa ccaaagcgtc ttattcaagt aacttatgat gcaatgatga aaggaattga agtagtgaga ccaggtgcta aactcggcga tatcgggtat gcaattcaaa gttatgccga gaaacataat tattctgtgg ttagagatta tacgggtcac ggtattggaa gagtttttca tgataaacct tcaatattaa attacggccg caacggcaca ggtctaacgt taaaagaagg aatgtttttt actgttgaac ctatgataaa tgcaggtaat tatgacacta tattaagtaa attagatgga tggacagtga ccacgcgtga taaatcatta tcggcacaat ttgagcatac tataggcgtg actaaagatg gctttgaaat atttacatta tcacctaaaa aacttgatta tcctccatac taatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for RiprA.00039.a.B1.PS00343
| PURIFICATION DATe: | 9/2/2009 |
| CONCENTRATION: | 18.91mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT IKIHTEKDFI KMRAAGKLAA ETLDFITDHV KPNVTTNSLN DLCHNFITSH NAIPAPLNYK GFPKSICTSI NHVVCHGIPN DKPLKNGDIV NIDVTVILDG WYGDTSRMYY VGDVAIKPKR LIQVTYDAMM KGIEVVRPGA KLGDIGYAIQ SYAEKHNYSV VRDYTGHGIG RVFHDKPSVL NYGRNGTGLT LKEGMFFTVE PMINAGNYDT ILSRLDGWTV TTRDKSLSAQ FEHTIGVTKD GFEIFTLSPK KLDYPPY
Validated NT Sequence
atcttactca cttagtatgg aggataatca agttttttag gtgataatgt aaatatttca aagccatctt tagtcacgcc tatagtatgc tcaaattgtg ccgataatga tttatcacgc gtggtcactg tccatccatc taatctactt aatatagtgt cataattacc tgcatttatc ataggttcaa cagtaaaaaa cattccttct tttaacgtta gacctgtgcc gttgcggccg taatttaata ctgaaggttt atcatgaaaa actcttccaa taccgtgacc cgtataatct ctaaccacag aataattatg tttctcggca taactttgaa ttgcataccc gatatcgccg agtttagcac ctggtctcac tacttcaatt cctttcatca ttgcatcata agttacttga ataagacgct ttggtttaat tgctacgtca ccgacgtaat acatacgact agtatcacca taccagccat ctaaaattac cgtaacatca atatttacta tatcaccatt ttttaatggt ttatcatttg gaattccgtg acaaactaca tgatttatag aagtacaaat cgattttgga aagcctttgt aatttaacgg tgcaggaatt gcattatgag aagtaataaa attatgacaa aggtcattca agctattagt agtgacatta ggtttgacgt gatcagtaat aaaatcgagt gtctctgcag ctaattttcc tgctgcccgc atctttataa aatctttttc agtgtggatt tttatagtca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMT IKIHTEKDFI KMRAAGKLAA ETLDFITDHV KPNVTTNSLN DLCHNFITSH NAIPAPLNYK GFPKSICTSI NHVVCHGIPN DKPLKNGDIV NIDVTVILDG WYGDTSRMYY VGDVAIKPKR LIQVTYDAMM KGIEVVRPGA KLGDIGYAIQ SYAEKHNYSV VRDYTGHGIG RVFHDKPSIL NYGRNGTGLT LKEGMFFTVE PMINAGNYDT ILSKLDGWTV TTRDKSLSAQ FEHTIGVTKD GFEIFTLSPK KLDYPPY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTA TAAAAATCCA CACTGAAAAA GATTTTATAA AGATGCGGGC AGCAGGAAAA TTAGCTGCAG AGACACTCGA TTTTATTACT GATCACGTCA AACCTAATGT CACTACTAAT AGCTTGAATG ACCTTTGTCA TAATTTTATT ACTTCTCATA ATGCAATTCC TGCACCGTTA AATTACAAAG GCTTTCCAAA ATCGATTTGT ACTTCTATAA ATCATGTAGT TTGTCACGGA ATTCCAAATG ATAAACCATT AAAAAATGGT GATATAGTAA ATATTGATGT TACGGTAATT TTAGATGGCT GGTATGGTGA TACTAGTCGT ATGTATTACG TCGGTGACGT AGCAATTAAA CCAAAGCGTC TTATTCAAGT AACTTATGAT GCAATGATGA AAGGAATTGA AGTAGTGAGA CCAGGTGCTA AACTCGGCGA TATCGGGTAT GCAATTCAAA GTTATGCCGA GAAACATAAT TATTCTGTGG TTAGAGATTA TACGGGTCAC GGTATTGGAA GAGTTTTTCA TGATAAACCT TCAATATTAA ATTACGGCCG CAACGGCACA GGTCTAACGT TAAAAGAAGG AATGTTTTTT ACTGTTGAAC CTATGATAAA TGCAGGTAAT TATGACACTA TATTAAGTAA ATTAGATGGA TGGACAGTGA CCACGCGTGA TAAATCATTA TCGGCACAAT TTGAGCATAC TATAGGCGTG ACTAAAGATG GCTTTGAAAT ATTTACATTA TCACCTAAAA AACTTGATTA TCCTCCATAC TAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT