RiprA.00030.a

ATP-dependent protease ATP-binding subunit

CENTER ID: RiprA.00030.a
ORGANISM: Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE: Endemic typhus
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
RiprA.00030.a.A2.GE27980 Created from Decode construct for feature RiprA.00030.a 1 399
RiprA.00030.a.A3.GE27981 Created from Decode construct for feature RiprA.00030.a 1 406
RiprA.00030.a.A4.GE27982 Created from Decode construct for feature RiprA.00030.a 59 425
RiprA.00030.a.A5.GE27983 Created from Decode construct for feature RiprA.00030.a 59 399
RiprA.00030.a.A6.GE27984 Created from Decode construct for feature RiprA.00030.a 59 406
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
RiprA.00030.a.A4.PW27842 Created from Decode construct for feature RiprA.00030.a 59 425
RiprA.00030.a.A6.PW27844 Created from Decode construct for feature RiprA.00030.a 59 406
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_128011
PATRIC ID: fig|272947.5.peg.714
RefSeq: NP_221053
UniProt: Q9ZCN1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVVDADKKEL ICSFCSKKQH EVKKLIAGPA VFICDECIDL CTDIMKEESK VALKQITASI PTPQKICKIL NDYVVGQDQA KKILAVAVYN HYKRLEYVQS GNNDVELNKS NILLIGPTGS GKTLLAQTLA KILDVPFTMA DATSLTEAGY VGEDVENILL RLLISAEFNI AKAQKGIIYI DEVDKIARKS DNPSITRDVS GEGVQQALLK IMEGTVASVP PQGGRKHPQQ DFVQLDTSNI LFICGGAFMG IDRIIRSRTN HSSIGFAANI NIDKEKSNNE ILKSLEIEDL TKFGLIPEFI GRLPIITTLD ELDKEALITI LTKPKNAIVK QYQKQFELDD ADLVIDYSAL EAIAEKALVK KTGARGLRSI LEHLLLDSMY KVAELKKQRV TITKEVVDGL IEPCMTSIIS TKLNKKQPII EDIPA
NT Sequence
ATGGTAGTAG ACGCTGATAA AAAAGAATTA ATTTGTTCGT TTTGCAGTAA AAAACAACAT GAAGTAAAAA AACTGATAGC AGGTCCTGCA GTATTTATTT GTGATGAATG TATAGATTTA TGTACAGATA TTATGAAAGA AGAGAGCAAA GTTGCTTTAA AGCAAATTAC TGCTTCAATT CCAACACCGC AAAAAATATG TAAAATTTTA AATGATTACG TAGTTGGACA AGACCAAGCT AAAAAGATTT TAGCGGTAGC GGTTTACAAT CATTATAAGA GACTTGAATA TGTACAATCT GGCAATAATG ATGTTGAGCT TAATAAGTCG AATATCTTAC TGATTGGTCC TACTGGTTCA GGAAAAACTT TACTAGCTCA AACGCTCGCT AAAATTTTAG ATGTACCTTT TACTATGGCA GATGCTACTT CTTTAACTGA AGCGGGTTAT GTCGGTGAAG ATGTTGAAAA TATATTACTA CGCTTATTAA TATCAGCTGA ATTTAATATA GCAAAAGCAC AAAAGGGTAT AATTTATATT GATGAGGTTG ATAAAATCGC TCGTAAATCG GATAATCCTT CTATTACGCG AGATGTATCA GGTGAAGGAG TGCAACAGGC ATTACTTAAA ATTATGGAAG GAACAGTTGC TTCTGTACCT CCTCAAGGTG GTAGAAAACA CCCACAACAA GATTTTGTAC AGTTAGATAC TTCAAATATC TTATTTATTT GTGGTGGCGC TTTTATGGGA ATTGATCGCA TTATTAGATC AAGAACTAAT CATAGCTCTA TAGGATTTGC AGCTAACATC AATATCGATA AAGAGAAAAG TAATAATGAA ATTCTTAAGT CTCTAGAGAT TGAAGATTTA ACTAAATTTG GTTTAATTCC TGAGTTTATC GGTAGGTTAC CAATTATTAC TACTTTAGAT GAGTTAGACA AAGAAGCTTT AATTACAATT CTCACTAAGC CTAAGAATGC TATAGTAAAA CAATATCAGA AACAATTTGA GCTTGATGAT GCTGATCTTG TAATTGACTA TTCAGCTCTA GAAGCTATTG CTGAAAAAGC TTTAGTTAAG AAGACGGGTG CAAGAGGTTT ACGTTCAATA CTTGAACATC TTTTACTAGA TAGTATGTAT AAAGTTGCTG AGCTTAAAAA ACAGCGTGTT ACTATTACAA AAGAGGTGGT GGATGGCTTA ATAGAACCGT GCATGACCTC TATCATTTCT ACAAAATTAA ATAAAAAGCA ACCGATTATT GAGGATATAC CTGCG
Details for RiprA.00030.a.A2.GE27980
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 46kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 62
Validated AA Sequence
MADATSLTEA GYVGEDVENI LLRLLISAEF NIAKAQKGII YIDEVDKIAR KSDNPSITRD VSGEGVQQAL LKIMEGTVAS VPPQGGRKHP QQDFVQLDTS NILFICGGAF MGIDRIIRSR TNHSSIGFAA NINIDKEKSN NEILKSLEIE DLTKFGLIPE FIGRLPIITT LDELDKEALI TILTKPKNAI VKQYQKQFEL DDADLVIDYS ALEAIAEKAL VKKTGARGLR SILEHLLLDS MYKVAELKKQ RVTITKEVVD G
Validated NT Sequence
tttcgggctt tgttagcagc cggatcctcg agaagcttgg ctgcagaact tgttcgtgct gtttattagc catccaccac ctcttttgta atagtaacac gctgtttttt aagctcagca actttataca tactatctag taaaagatgt tcaagtattg aacgtaaacc tcttgcaccc gtcttcttaa ctaaagcttt ttcagcaata gcttctagag ctgaatagtc aattacaaga tcagcatcat caagctcaaa ttgtttctga tattgtttta ctatagcatt cttaggctta gtgagaattg taattaaagc ttctttgtct aactcatcta aagtagtaat aattggtaac ctaccgataa actcaggaat taaaccaaat ttagttaaat cttcaatctc tagagactta agaatttcat tattactttt ctctttatcg atattgatgt tagctgcaaa tcctatagag ctatgattag ttcttgatct aataatgcga tcaattccca taaaagcgcc accacaaata aataagatat ttgaagtatc taactgtaca aaatcttgtt gtgggtgttt tctaccacct tgaggaggta cagaagcaac tgttccttcc ataattttaa gtaatgcctg ttgcactcct tcacctgata catctcgcgt aatagaagga ttatccgatt tacgagcgat tttatcaacc tcatcaatat aaattatacc cttttgtgct tttgctatat taaattcagc tgatattaat aagcgtagta atatattttc aacatcttca ccgacataac ccgcttcagt taaagaagta gcatctgcca tagtaaaagg tacatctaaa attttagcga gcgtttgagc tagtaaagtt tttcctgaac cagtagganc aatcagtaag anattcgact tattaagctc aacatcatta ttgccagatt gtacatattc aagtctctta taatgattgn aaaccgctac cgctaantct ttttagcttg gncntgtcca actacgtaat catttaaaat tttannnnnt ttttgngnng nnnnnantgn agcagtantt nnnntnnaag caactttgcn cnnnnnnttn nnnantcngt ncnnnaannn nnnnnnnnnn nnncnnnnnn nnnnnnnnnn ggccnnnntn nnannntnnn nnnnnnnnnn nnnnntttt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVDADKKE LICSFCSKKQ HEVKKLIAGP AVFICDECID LCTDIMKEES KVALKQITAS IPTPQKICKI LNDYVVGQDQ AKKILAVAVY NHYKRLEYVQ SGNNDVELNK SNILLIGPTG SGKTLLAQTL AKILDVPFTM ADATSLTEAG YVGEDVENIL LRLLISAEFN IAKAQKGIIY IDEVDKIARK SDNPSITRDV SGEGVQQALL KIMEGTVASV PPQGGRKHPQ QDFVQLDTSN ILFICGGAFM GIDRIIRSRT NHSSIGFAAN INIDKEKSNN EILKSLEIED LTKFGLIPEF IGRLPIITTL DELDKEALIT ILTKPKNAIV KQYQKQFELD DADLVIDYSA LEAIAEKALV KKTGARGLRS ILEHLLLDSM YKVAELKKQR VTITKEVVDG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtagtagac gctgataaaa aagaattaat ttgttcgttt tgcagtaaaa aacaacatga agtaaaaaaa ctgatagcag gtcctgcagt atttatttgt gatgaatgta tagatttatg tacagatatt atgaaagaag agagcaaagt tgctttaaag caaattactg cttcaattcc aacaccgcaa aaaatatgta aaattttaaa tgattacgta gttggacaag accaagctaa aaagatttta gcggtagcgg tttacaatca ttataagaga cttgaatatg tacaatctgg caataatgat gttgagctta ataagtcgaa tatcttactg attggtccta ctggttcagg aaaaacttta ctagctcaaa cgctcgctaa aattttagat gtacctttta ctatggcaga tgctacttct ttaactgaag cgggttatgt cggtgaagat gttgaaaata tattactacg cttattaata tcagctgaat ttaatatagc aaaagcacaa aagggtataa tttatattga tgaggttgat aaaatcgctc gtaaatcgga taatccttct attacgcgag atgtatcagg tgaaggagtg caacaggcat tacttaaaat tatggaagga acagttgctt ctgtacctcc tcaaggtggt agaaaacacc cacaacaaga ttttgtacag ttagatactt caaatatctt atttatttgt ggtggcgctt ttatgggaat tgatcgcatt attagatcaa gaactaatca tagctctata ggatttgcag ctaacatcaa tatcgataaa gagaaaagta ataatgaaat tcttaagtct ctagagattg aagatttaac taaatttggt ttaattcctg agtttatcgg taggttacca attattacta ctttagatga gttagacaaa gaagctttaa ttacaattct cactaagcct aagaatgcta tagtaaaaca atatcagaaa caatttgagc ttgatgatgc tgatcttgta attgactatt cagctctaga agctattgct gaaaaagctt tagttaagaa gacgggtgca agaggtttac gttcaatact tgaacatctt ttactagata gtatgtataa agttgctgag cttaaaaaac agcgtgttac tattacaaaa gaggtggtgg atggcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for RiprA.00030.a.A3.GE27981
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVVDADKKE LICSFCSKKQ HEVKKLIAGP AVFICDECID LCTDIMKEES KVALKQITAS IPTPQKICKI LNDYVVGQDQ AKKILAVAVY NHYKRLEYVQ SGNNDVELNK SNILLIGPTG SGKTLLAQTL AKILDVPFTM ADATSLTEAG YVGEDVENIL LRLLISAEFN IAKAQKGIIY IDEVDKIARK SDNPSITRDV SGEGVQQALL KIMEGTVASV PPQGGRKHPQ QDFVQLDTSN ILFICGGAFM GIDRIIRSRT NHSSIGFAAN INIDKEKSNN EILKSLEIED LTKFGLIPEF IGRLPIITTL DELDKEALIT ILTKPKNAIV KQYQKQFELD DADLVIDYSA LEAIAEKALV KKTGARGLRS ILEHLLLDSM YKVAELKKQR VTITKEVVDG LIEPCM
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtag tagacgctga taaaaaagaa ttaatttgtt cgttttgcag taaaaaacaa catgaagtaa aaaaactgat agcaggtcct gcagtattta tttgtgatga atgtatagat ttatgtacag atattatgaa agaagagagc aaagttgctt taaagcaaat tactgcttca attccaacac cgcaaaaaat atgtaaaatt ttaaatgatt acgtagttgg acaagaccaa gctaaaaaga ttttagcggt agcggtttac aatcattata agagacttga atatgtacaa tctggcaata atgatgttga gcttaataag tcgaatatct tactgattgg tcctactggt tcaggaaaaa ctttactagc tcaaacgctc gctaaaattt tagatgtacc ttttactatg gcagatgcta cttctttaac tgaagcgggt tatgtcggtg aagatgttga aaatatatta ctacgcttat taatatcagc tgaatttaat atagcaaaag cacaaaaggg tataatttat attgatgagg ttgataaaat cgctcgtaaa tcggataatc cttctattac gcgagatgta tcaggtgaag gagtgcaaca ggcattactt aaaattatgg aaggaacagt tgcttctgta cctcctcaag gtggtagaaa acacccacaa caagattttg tacagttaga tacttcaaat atcttattta tttgtggtgg cgcttttatg ggaattgatc gcattattag atcaagaact aatcatagct ctataggatt tgcagctaac atcaatatcg ataaagagaa aagtaataat gaaattctta agtctctaga gattgaagat ttaactaaat ttggtttaat tcctgagttt atcggtaggt taccaattat tactacttta gatgagttag acaaagaagc tttaattaca attctcacta agcctaagaa tgctatagta aaacaatatc agaaacaatt tgagcttgat gatgctgatc ttgtaattga ctattcagct ctagaagcta ttgctgaaaa agctttagtt aagaagacgg gtgcaagagg tttacgttca atacttgaac atcttttact agatagtatg tataaagttg ctgagcttaa aaaacagcgt gttactatta caaaagaggt ggtggatggc ttaatagaac cgtgcatgac c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVDADKKE LICSFCSKKQ HEVKKLIAGP AVFICDECID LCTDIMKEES KVALKQITAS IPTPQKICKI LNDYVVGQDQ AKKILAVAVY NHYKRLEYVQ SGNNDVELNK SNILLIGPTG SGKTLLAQTL AKILDVPFTM ADATSLTEAG YVGEDVENIL LRLLISAEFN IAKAQKGIIY IDEVDKIARK SDNPSITRDV SGEGVQQALL KIMEGTVASV PPQGGRKHPQ QDFVQLDTSN ILFICGGAFM GIDRIIRSRT NHSSIGFAAN INIDKEKSNN EILKSLEIED LTKFGLIPEF IGRLPIITTL DELDKEALIT ILTKPKNAIV KQYQKQFELD DADLVIDYSA LEAIAEKALV KKTGARGLRS ILEHLLLDSM YKVAELKKQR VTITKEVVDG LIEPCMT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtagtagac gctgataaaa aagaattaat ttgttcgttt tgcagtaaaa aacaacatga agtaaaaaaa ctgatagcag gtcctgcagt atttatttgt gatgaatgta tagatttatg tacagatatt atgaaagaag agagcaaagt tgctttaaag caaattactg cttcaattcc aacaccgcaa aaaatatgta aaattttaaa tgattacgta gttggacaag accaagctaa aaagatttta gcggtagcgg tttacaatca ttataagaga cttgaatatg tacaatctgg caataatgat gttgagctta ataagtcgaa tatcttactg attggtccta ctggttcagg aaaaacttta ctagctcaaa cgctcgctaa aattttagat gtacctttta ctatggcaga tgctacttct ttaactgaag cgggttatgt cggtgaagat gttgaaaata tattactacg cttattaata tcagctgaat ttaatatagc aaaagcacaa aagggtataa tttatattga tgaggttgat aaaatcgctc gtaaatcgga taatccttct attacgcgag atgtatcagg tgaaggagtg caacaggcat tacttaaaat tatggaagga acagttgctt ctgtacctcc tcaaggtggt agaaaacacc cacaacaaga ttttgtacag ttagatactt caaatatctt atttatttgt ggtggcgctt ttatgggaat tgatcgcatt attagatcaa gaactaatca tagctctata ggatttgcag ctaacatcaa tatcgataaa gagaaaagta ataatgaaat tcttaagtct ctagagattg aagatttaac taaatttggt ttaattcctg agtttatcgg taggttacca attattacta ctttagatga gttagacaaa gaagctttaa ttacaattct cactaagcct aagaatgcta tagtaaaaca atatcagaaa caatttgagc ttgatgatgc tgatcttgta attgactatt cagctctaga agctattgct gaaaaagctt tagttaagaa gacgggtgca agaggtttac gttcaatact tgaacatctt ttactagata gtatgtataa agttgctgag cttaaaaaac agcgtgttac tattacaaaa gaggtggtgg atggcttaat agaaccgtgc atgaccaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for RiprA.00030.a.A4.GE27982
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 42kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIPTPQKI CKILNDYVVG QDQAKKILAV AVYNHYKRLE YVQSGNNDVE LNKSNILLIG PTGSGKTLLA QTLAKILDVP FTMADATSLT EAGYVGEDVE NILLRLLISA EFNIAKAQKG IIYIDEVDKI ARKSDNPSIT RDVSGEGVQQ ALLKIMEGTV ASVPPQGGRK HPQQDFVQLD TSNILFICGG AFMGIDRIIR SRTNHSSIGF AANINIDKEK SNNEILKSLE IEDLTKFGLI PEFIGRLPII TTLDELDKEA LITILTKPKN AIVKQYQKQF ELDDADLVID YSALEAIAEK ALVKKTGARG LRSILEHLLL DSMYKVAELK KQRVTITKEV VDGLIEPCMT SIISTKLNKK QPIIEDIPA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcaattcca acaccgcaaa aaatatgtaa aattttaaat gattacgtag ttggacaaga ccaagctaaa aagattttag cggtagcggt ttacaatcat tataagagac ttgaatatgt acaatctggc aataatgatg ttgagcttaa taagtcgaat atcttactga ttggtcctac tggttcagga aaaactttac tagctcaaac gctcgctaaa attttagatg taccttttac tatggcagat gctacttctt taactgaagc gggttatgtc ggtgaagatg ttgaaaatat attactacgc ttattaatat cagctgaatt taatatagca aaagcacaaa agggtataat ttatattgat gaggttgata aaatcgctcg taaatcggat aatccttcta ttacgcgaga tgtatcaggt gaaggagtgc aacaggcatt acttaaaatt atggaaggaa cagttgcttc tgtacctcct caaggtggta gaaaacaccc acaacaagat tttgtacagt tagatacttc aaatatctta tttatttgtg gtggcgcttt tatgggaatt gatcgcatta ttagatcaag aactaatcat agctctatag gatttgcagc taacatcaat atcgataaag agaaaagtaa taatgaaatt cttaagtctc tagagattga agatttaact aaatttggtt taattcctga gtttatcggt aggttaccaa ttattactac tttagatgag ttagacaaag aagctttaat tacaattctc actaagccta agaatgctat agtaaaacaa tatcagaaac aatttgagct tgatgatgct gatcttgtaa ttgactattc agctctagaa gctattgctg aaaaagcttt agttaagaag acgggtgcaa gaggtttacg ttcaatactt gaacatcttt tactagatag tatgtataaa gttgctgagc ttaaaaaaca gcgtgttact attacaaaag aggtggtgga tggcttaata gaaccgtgca tgacctctat catttctaca aaattaaata aaaagcaacc gattattgag gatatacctg cgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for RiprA.00030.a.A5.GE27983
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 71
Validated AA Sequence
MADATSLTEA GYVGEDVENI LLRLLISAEF NIAKAQKGII YIDEVDKIAR KSDNPSITRD VSGEGVQQAL LKIMEGTVAS VPPQGGRKHP QQDFVQLDTS NILFICGGAF MGIDRIIRSR TNHSSIGFAA NINIDKEKSN NEILKSLEIE DLTKFGLIPE FIGRLPIITT LDELDKEALI TILTKPKNAI VKQYQKQFEL DDADLVIDYS ALEAIAEKAL VKKTGARGLR SILEHLLLDS MYKVAELKKQ RVTITKEVVD G
Validated NT Sequence
tnnttcgngc tttgttagca gccggatcct cgagaagctt ggctgcagaa cttgttcgtg ctgtttatta gccatccacc acctcttttg taatagtaac acgctgtttt ttaagctcag caactttata catactatct agtaaaagat gttcaagtat tgaacgtaaa cctcttgcac ccgtcttctt aactaaagct ttttcagcaa tagcttctag agctgaatag tcaattacaa gatcagcatc atcaagctca aattgtttct gatattgttt tactatagca ttcttaggct tagtgagaat tgtaattaaa gcttctttgt ctaactcatc taaagtagta ataattggta acctaccgat aaactcagga attaaaccaa atttagttaa atcttcaatc tctagagact taagaatttc attattactt ttctctttat cgatattgat gttagctgca aatcctatag agctatgatt agttcttgat ctaataatgc gatcaattcc cataaaagcg ccaccacaaa taaataagat atttgaagta tctaactgta caaaatcttg ttgtgggtgt tttctaccac cttgaggagg tacagaagca actgttcctt ccataatttt aagtaatgcc tgttgcactc cttcacctga tacatctcgc gtaatagaag gattatccga tttacgagcg attttatcaa cctcatcaat ataaattata cccttttgtg cttttgctat attaaattca gctgatatta ataagcgtag taatatattt tcaacatctt caccgacata acccgcttca gttaaagaag tagcatctgc catagtaaaa ggnacatcta aaattttagc gagcgtttga gctagtaaag tttttcctga accagtanga ccnatcagta agatattcga cttattaagc tcnacatcat tattgccaga ttgtacatat tcaagtctct tataatgatn gnaaaccgct accgctaaat ctttttagct ngnntccaac tacgtaatca tttaaaattt tacatatttt nnngngtnnn nngacatcnn nnnnncnngg nnntganctn nggnnccnna tnangngatg gnnanancca nggnnnnnnn nncnnnnnan nnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIPTPQKI CKILNDYVVG QDQAKKILAV AVYNHYKRLE YVQSGNNDVE LNKSNILLIG PTGSGKTLLA QTLAKILDVP FTMADATSLT EAGYVGEDVE NILLRLLISA EFNIAKAQKG IIYIDEVDKI ARKSDNPSIT RDVSGEGVQQ ALLKIMEGTV ASVPPQGGRK HPQQDFVQLD TSNILFICGG AFMGIDRIIR SRTNHSSIGF AANINIDKEK SNNEILKSLE IEDLTKFGLI PEFIGRLPII TTLDELDKEA LITILTKPKN AIVKQYQKQF ELDDADLVID YSALEAIAEK ALVKKTGARG LRSILEHLLL DSMYKVAELK KQRVTITKEV VDG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcaattcca acaccgcaaa aaatatgtaa aattttaaat gattacgtag ttggacaaga ccaagctaaa aagattttag cggtagcggt ttacaatcat tataagagac ttgaatatgt acaatctggc aataatgatg ttgagcttaa taagtcgaat atcttactga ttggtcctac tggttcagga aaaactttac tagctcaaac gctcgctaaa attttagatg taccttttac tatggcagat gctacttctt taactgaagc gggttatgtc ggtgaagatg ttgaaaatat attactacgc ttattaatat cagctgaatt taatatagca aaagcacaaa agggtataat ttatattgat gaggttgata aaatcgctcg taaatcggat aatccttcta ttacgcgaga tgtatcaggt gaaggagtgc aacaggcatt acttaaaatt atggaaggaa cagttgcttc tgtacctcct caaggtggta gaaaacaccc acaacaagat tttgtacagt tagatacttc aaatatctta tttatttgtg gtggcgcttt tatgggaatt gatcgcatta ttagatcaag aactaatcat agctctatag gatttgcagc taacatcaat atcgataaag agaaaagtaa taatgaaatt cttaagtctc tagagattga agatttaact aaatttggtt taattcctga gtttatcggt aggttaccaa ttattactac tttagatgag ttagacaaag aagctttaat tacaattctc actaagccta agaatgctat agtaaaacaa tatcagaaac aatttgagct tgatgatgct gatcttgtaa ttgactattc agctctagaa gctattgctg aaaaagcttt agttaagaag acgggtgcaa gaggtttacg ttcaatactt gaacatcttt tactagatag tatgtataaa gttgctgagc ttaaaaaaca gcgtgttact attacaaaag aggtggtgga tggcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for RiprA.00030.a.A6.GE27984
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 69
Validated AA Sequence
MADATSLTEA GYVGEXVENI LLRLLISAEF NIAKAQKGII YIDEVDKIXR KSDNPSITRD VSGEGXQQAL LXIMXXTXAS VPPXGGRKHP QQDFVQLDTS NILFICGGAF MGIDXIIRSR TNHSSIGFAA NINIDKEKSN NEILKSLEIE DLTKFGLIPE FIGRLPIITT LDELDKEALI TILTKPKNAI VKQYQKQFEL DDADLVIDYS ALEAIAEKAL VKKTGARGLR SILEHLLLDS MYKVAELKKQ RVTITK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcanaccca gggtcctggt tcgatgtcaa ttccaacacc gcanaaatat gtaaaatttt aaatgattac gtagttggac aagaccaagc taaaaagatt ttagcggtag cggtttacaa tcattataag agacttgaat atgtacaatc tggcaataat gatgttgagc ttaataagtc gaatatctta ctgattggtc ctactggttc aggaaaaact ttactagctc aaacgctcgc taaaatttta gatgtacctt ttactatggc agatgctact tctttaactg aagcgggtta tgtcggtgaa natgttgaaa atatattact acgcttatta atatcngctg aatttaatat agcaaaagca caaaagggta taatttatat tgatgaggtt gataaaatcn ctcgtaaatc ggataatcct tctattacgc gagatgtatc aggtgaagga ntgcaacagg cattacttan aattatggan ngaacanntg cttctgtacc tccncnnggt ggtagaaaac acccacaaca agattttgta cagttagata cttcaaatat cttatttatt tgtggtggcg cttttatggg aattgatcnc attattagat caagaactaa tcatagctct ataggatttg cagctaacat caatatcgat aaagagaaaa gtaataatga aattcttaag tctctagaga ttgaagattt aactaaattt ggtttaattc ctgagtttat cggtaggtta ccaattatta ctactttaga tgagttagac aaagaagctt taattacaat tctcactaag cctaagaatg ctatagtaaa acaatatcag aaacaatttg agcttgatga tgctgatctt gtaattgact attcagctct agaagctatt gctgaaaaag cnttagttaa gaagacgggt gcaagaggtt tacgttcaat acttgaacat cttttactag atagtatgta taaagttgct gagcttaaaa aacagcgtgt tactattaca aaaga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIPTPQKI CKILNDYVVG QDQAKKILAV AVYNHYKRLE YVQSGNNDVE LNKSNILLIG PTGSGKTLLA QTLAKILDVP FTMADATSLT EAGYVGEDVE NILLRLLISA EFNIAKAQKG IIYIDEVDKI ARKSDNPSIT RDVSGEGVQQ ALLKIMEGTV ASVPPQGGRK HPQQDFVQLD TSNILFICGG AFMGIDRIIR SRTNHSSIGF AANINIDKEK SNNEILKSLE IEDLTKFGLI PEFIGRLPII TTLDELDKEA LITILTKPKN AIVKQYQKQF ELDDADLVID YSALEAIAEK ALVKKTGARG LRSILEHLLL DSMYKVAELK KQRVTITKEV VDGLIEPCMT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcaattcca acaccgcaaa aaatatgtaa aattttaaat gattacgtag ttggacaaga ccaagctaaa aagattttag cggtagcggt ttacaatcat tataagagac ttgaatatgt acaatctggc aataatgatg ttgagcttaa taagtcgaat atcttactga ttggtcctac tggttcagga aaaactttac tagctcaaac gctcgctaaa attttagatg taccttttac tatggcagat gctacttctt taactgaagc gggttatgtc ggtgaagatg ttgaaaatat attactacgc ttattaatat cagctgaatt taatatagca aaagcacaaa agggtataat ttatattgat gaggttgata aaatcgctcg taaatcggat aatccttcta ttacgcgaga tgtatcaggt gaaggagtgc aacaggcatt acttaaaatt atggaaggaa cagttgcttc tgtacctcct caaggtggta gaaaacaccc acaacaagat tttgtacagt tagatacttc aaatatctta tttatttgtg gtggcgcttt tatgggaatt gatcgcatta ttagatcaag aactaatcat agctctatag gatttgcagc taacatcaat atcgataaag agaaaagtaa taatgaaatt cttaagtctc tagagattga agatttaact aaatttggtt taattcctga gtttatcggt aggttaccaa ttattactac tttagatgag ttagacaaag aagctttaat tacaattctc actaagccta agaatgctat agtaaaacaa tatcagaaac aatttgagct tgatgatgct gatcttgtaa ttgactattc agctctagaa gctattgctg aaaaagcttt agttaagaag acgggtgcaa gaggtttacg ttcaatactt gaacatcttt tactagatag tatgtataaa gttgctgagc ttaaaaaaca gcgtgttact attacaaaag aggtggtgga tggcttaata gaaccgtgca tgaccaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for RiprA.00030.a.A4.PW27842
PURIFICATION DATe: 2/8/2010
CONCENTRATION: 24.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIPTPQKI CKILNDYVVG QDQAKKILAV AVYNHYKRLE YVQSGNNDVE LNKSNILLIG PTGSGKTLLA QTLAKILDVP FTMADATSLT EAGYVGEDVE NILLRLLISA EFNIAKAQKG IIYIDEVDKI ARKSDNPSIT RDVSGEGVQQ ALLKIMEGTV ASVPPQGGRK HPQQDFVQLD TSNILFICGG AFMGIDRIIR SRTNHSSIGF AANINIDKEK SNNEILKSLE IEDLTKFGLI PEFIGRLPII TTLDELDKEA LITILTKPKN AIVKQYQKQF ELDDADLVID YSALEAIAEK ALVKKTGARG LRSILEHLLL DSMYKVAELK KQRVTITKEV VDGLIEPCMT SIISTKLNKK QPIIEDIPA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAATTCCA ACACCGCAAA AAATATGTAA AATTTTAAAT GATTACGTAG TTGGACAAGA CCAAGCTAAA AAGATTTTAG CGGTAGCGGT TTACAATCAT TATAAGAGAC TTGAATATGT ACAATCTGGC AATAATGATG TTGAGCTTAA TAAGTCGAAT ATCTTACTGA TTGGTCCTAC TGGTTCAGGA AAAACTTTAC TAGCTCAAAC GCTCGCTAAA ATTTTAGATG TACCTTTTAC TATGGCAGAT GCTACTTCTT TAACTGAAGC GGGTTATGTC GGTGAAGATG TTGAAAATAT ATTACTACGC TTATTAATAT CAGCTGAATT TAATATAGCA AAAGCACAAA AGGGTATAAT TTATATTGAT GAGGTTGATA AAATCGCTCG TAAATCGGAT AATCCTTCTA TTACGCGAGA TGTATCAGGT GAAGGAGTGC AACAGGCATT ACTTAAAATT ATGGAAGGAA CAGTTGCTTC TGTACCTCCT CAAGGTGGTA GAAAACACCC ACAACAAGAT TTTGTACAGT TAGATACTTC AAATATCTTA TTTATTTGTG GTGGCGCTTT TATGGGAATT GATCGCATTA TTAGATCAAG AACTAATCAT AGCTCTATAG GATTTGCAGC TAACATCAAT ATCGATAAAG AGAAAAGTAA TAATGAAATT CTTAAGTCTC TAGAGATTGA AGATTTAACT AAATTTGGTT TAATTCCTGA GTTTATCGGT AGGTTACCAA TTATTACTAC TTTAGATGAG TTAGACAAAG AAGCTTTAAT TACAATTCTC ACTAAGCCTA AGAATGCTAT AGTAAAACAA TATCAGAAAC AATTTGAGCT TGATGATGCT GATCTTGTAA TTGACTATTC AGCTCTAGAA GCTATTGCTG AAAAAGCTTT AGTTAAGAAG ACGGGTGCAA GAGGTTTACG TTCAATACTT GAACATCTTT TACTAGATAG TATGTATAAA GTTGCTGAGC TTAAAAAACA GCGTGTTACT ATTACAAAAG AGGTGGTGGA TGGCTTAATA GAACCGTGCA TGACCTCTAT CATTTCTACA AAATTAAATA AAAAGCAACC GATTATTGAG GATATACCTG CGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for RiprA.00030.a.A6.PW27844
PURIFICATION DATe: 2/8/2010
CONCENTRATION: 14.62mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 69
Protocol Notes
notes unavailable
Validated AA Sequence
MADATSLTEA GYVGEXVENI LLRLLISAEF NIAKAQKGII YIDEVDKIXR KSDNPSITRD VSGEGXQQAL LXIMXXTXAS VPPXGGRKHP QQDFVQLDTS NILFICGGAF MGIDXIIRSR TNHSSIGFAA NINIDKEKSN NEILKSLEIE DLTKFGLIPE FIGRLPIITT LDELDKEALI TILTKPKNAI VKQYQKQFEL DDADLVIDYS ALEAIAEKAL VKKTGARGLR SILEHLLLDS MYKVAELKKQ RVTITK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcanaccca gggtcctggt tcgatgtcaa ttccaacacc gcanaaatat gtaaaatttt aaatgattac gtagttggac aagaccaagc taaaaagatt ttagcggtag cggtttacaa tcattataag agacttgaat atgtacaatc tggcaataat gatgttgagc ttaataagtc gaatatctta ctgattggtc ctactggttc aggaaaaact ttactagctc aaacgctcgc taaaatttta gatgtacctt ttactatggc agatgctact tctttaactg aagcgggtta tgtcggtgaa natgttgaaa atatattact acgcttatta atatcngctg aatttaatat agcaaaagca caaaagggta taatttatat tgatgaggtt gataaaatcn ctcgtaaatc ggataatcct tctattacgc gagatgtatc aggtgaagga ntgcaacagg cattacttan aattatggan ngaacanntg cttctgtacc tccncnnggt ggtagaaaac acccacaaca agattttgta cagttagata cttcaaatat cttatttatt tgtggtggcg cttttatggg aattgatcnc attattagat caagaactaa tcatagctct ataggatttg cagctaacat caatatcgat aaagagaaaa gtaataatga aattcttaag tctctagaga ttgaagattt aactaaattt ggtttaattc ctgagtttat cggtaggtta ccaattatta ctactttaga tgagttagac aaagaagctt taattacaat tctcactaag cctaagaatg ctatagtaaa acaatatcag aaacaatttg agcttgatga tgctgatctt gtaattgact attcagctct agaagctatt gctgaaaaag cnttagttaa gaagacgggt gcaagaggtt tacgttcaat acttgaacat cttttactag atagtatgta taaagttgct gagcttaaaa aacagcgtgt tactattaca aaaga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIPTPQKI CKILNDYVVG QDQAKKILAV AVYNHYKRLE YVQSGNNDVE LNKSNILLIG PTGSGKTLLA QTLAKILDVP FTMADATSLT EAGYVGEDVE NILLRLLISA EFNIAKAQKG IIYIDEVDKI ARKSDNPSIT RDVSGEGVQQ ALLKIMEGTV ASVPPQGGRK HPQQDFVQLD TSNILFICGG AFMGIDRIIR SRTNHSSIGF AANINIDKEK SNNEILKSLE IEDLTKFGLI PEFIGRLPII TTLDELDKEA LITILTKPKN AIVKQYQKQF ELDDADLVID YSALEAIAEK ALVKKTGARG LRSILEHLLL DSMYKVAELK KQRVTITKEV VDGLIEPCMT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAATTCCA ACACCGCAAA AAATATGTAA AATTTTAAAT GATTACGTAG TTGGACAAGA CCAAGCTAAA AAGATTTTAG CGGTAGCGGT TTACAATCAT TATAAGAGAC TTGAATATGT ACAATCTGGC AATAATGATG TTGAGCTTAA TAAGTCGAAT ATCTTACTGA TTGGTCCTAC TGGTTCAGGA AAAACTTTAC TAGCTCAAAC GCTCGCTAAA ATTTTAGATG TACCTTTTAC TATGGCAGAT GCTACTTCTT TAACTGAAGC GGGTTATGTC GGTGAAGATG TTGAAAATAT ATTACTACGC TTATTAATAT CAGCTGAATT TAATATAGCA AAAGCACAAA AGGGTATAAT TTATATTGAT GAGGTTGATA AAATCGCTCG TAAATCGGAT AATCCTTCTA TTACGCGAGA TGTATCAGGT GAAGGAGTGC AACAGGCATT ACTTAAAATT ATGGAAGGAA CAGTTGCTTC TGTACCTCCT CAAGGTGGTA GAAAACACCC ACAACAAGAT TTTGTACAGT TAGATACTTC AAATATCTTA TTTATTTGTG GTGGCGCTTT TATGGGAATT GATCGCATTA TTAGATCAAG AACTAATCAT AGCTCTATAG GATTTGCAGC TAACATCAAT ATCGATAAAG AGAAAAGTAA TAATGAAATT CTTAAGTCTC TAGAGATTGA AGATTTAACT AAATTTGGTT TAATTCCTGA GTTTATCGGT AGGTTACCAA TTATTACTAC TTTAGATGAG TTAGACAAAG AAGCTTTAAT TACAATTCTC ACTAAGCCTA AGAATGCTAT AGTAAAACAA TATCAGAAAC AATTTGAGCT TGATGATGCT GATCTTGTAA TTGACTATTC AGCTCTAGAA GCTATTGCTG AAAAAGCTTT AGTTAAGAAG ACGGGTGCAA GAGGTTTACG TTCAATACTT GAACATCTTT TACTAGATAG TATGTATAAA GTTGCTGAGC TTAAAAAACA GCGTGTTACT ATTACAAAAG AGGTGGTGGA TGGCTTAATA GAACCGTGCA TGACCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA