RiprA.00010.b
3-ketoacyl-(acyl-carrier-protein) reductase
| CENTER ID: | RiprA.00010.b |
| ORGANISM: | Rickettsia prowazekii str. Madrid E |
| ASSOCIATED DISEASE: | Endemic typhus |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiprA.00010.b.A1.GE25600 | Full length( RiprA.00010.b ) | 1 | 241 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiprA.00010.b.A1.PS00093 | Full length( RiprA.00010.b ) | 1 | 241 |
Structures
3F9I
DEPOSITED: 11/13/2008
DETERMINATION: XRay
CLONE: RiprA.00010.b.A1.GE25600
PROTEIN: RiprA.00010.b.A1.PS00093
Publications by SSGCID
Structure of 3-ketoacyl-(acyl-carrier-protein) reductase from Rickettsia prowazekii at 2.25 A resolution.
Abendroth J, Myler PJ, Napuli A, Olsen C, Phan IQ, Stacy R, Staker BL, Subramanian S, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1118-22
PMID: 21904060; PMCID: PMC3169412
PDB:
3F9I
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126618 |
| PATRIC ID: | fig|272947.5.peg.798 |
| RefSeq: | NP_221114.1 |
| UniProt: | P50941 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIDLTGKTSL ITGASSGIGS AIARLLHKLG SKVIISGSNE EKLKSLGNAL KDNYTIEVCN LANKEECSNL ISKTSNLDIL VCNAGITSDT LAIRMKDQDF DKVIDINLKA NFILNREAIK KMIQKRYGRI INISSIVGIA GNPGQANYCA SKAGLIGMTK SLSYEVATRG ITVNAVAPGF IKSDMTDKLN EKQREAIVQK IPLGTYGIPE DVAYAVAFLA SNNASYITGQ TLHVNGGMLM V
NT Sequence
ATGATTGATT TAACAGGTAA AACTTCCTTA ATTACAGGTG CTTCAAGTGG CATAGGAAGT GCCATAGCGC GGTTACTACA TAAACTCGGG AGCAAGGTAA TTATTAGCGG TAGTAATGAA GAAAAATTAA AATCACTTGG CAATGCTTTA AAAGATAATT ATACCATAGA AGTATGTAAT CTTGCGAATA AGGAAGAATG TAGTAATTTA ATATCTAAAA CATCAAATTT AGATATTTTA GTATGTAATG CCGGTATTAC TAGTGATACA CTCGCAATTA GAATGAAAGA TCAAGATTTC GACAAAGTTA TTGATATTAA TTTGAAAGCC AATTTTATTT TAAATCGTGA AGCAATAAAA AAAATGATAC AAAAAAGGTA TGGACGCATT ATTAACATAT CTTCAATAGT AGGAATTGCA GGTAACCCAG GACAGGCTAA TTATTGCGCT TCTAAAGCAG GTTTAATAGG TATGACCAAA TCGCTATCTT ACGAAGTTGC AACTAGAGGG ATTACGGTTA ATGCAGTAGC ACCAGGATTT ATTAAATCAG ATATGACTGA TAAACTCAAT GAAAAACAAC GAGAAGCAAT AGTACAAAAA ATTCCACTAG GTACTTATGG TATACCAGAA GACGTTGCAT ACGCAGTCGC ATTTTTAGCA AGCAATAATG CATCATATAT TACTGGTCAA ACTCTACATG TAAACGGTGG TATGTTAATG GTA
Details for RiprA.00010.b.A1.GE25600
| HARVESTED ON: | 9/10/2008 |
| SEQUENCED ON: | 7/28/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIDLTGKTS LITGASSGIG SAIARLLHKL GSKVIISGSN EEKLKSLGNA LKDNYTIEVC NLANKEECSN LISKTSNLDI LVCNAGITSD TLAIRMKDQD FDKVIDINLK ANFILNREAI KKMIQKRYGR IINISSIVGI AGNPGQANYC ASKAGLIGMT KSLSYEVATR GITVNAVAPG FIKSDMTDKL NEKQREAIVQ KIPLGTYGIP EDVAYAVAFL ASNNASYITG QTLHVNGGML MV
Validated NT Sequence
taatacgact cactataggg agaccacaan ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgattga tttaacaggt aaaacttcct taattacagg tgcttcaagt ggcataggaa gtgccatagc gcggttacta cataaactcg ggagcaaggt aattattagc ggtagtaatg aagaaaaatt aaaatcactt ggcaatgctt taaaagataa ttataccata gaagtatgta atcttgcgaa taaggaagaa tgtagtaatt taatatctaa aacatcaaat ttagatattt tagtatgtaa tgccggtatt actagtgata cactcgcaat tagaatgaaa gatcaagatt tcgacaaagt tattgatatt aatttgaaag ccaattttat tttaaatcgt gaagcaataa aaaaaatgat acaaaaaagg tatggacgca ttattaacat atcttcaata gtaggaattg caggtaaccc aggacaggct aattattgcg cttctaaagc aggtttaata ggtatgacca aatcgctatc ttacgaagtt gcaactagag ggattacggt taatgcagta gcaccaggat ttattaaatc agatatgact gataaactca atgaaaaaca acgagaagca atagtacaaa aaattccact aggtacttat ggtataccag aagacgttgc atacgcagtc gcatttttag caagcaataa tgcatcatat attactggtc aaactttaca tgtaaacggt ggtatgttaa tggtataata aacagcacga acaagttctg cagccaagct tctcgaggnt ccggctgcta acaaagcccg aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIDLTGKTS LITGASSGIG SAIARLLHKL GSKVIISGSN EEKLKSLGNA LKDNYTIEVC NLANKEECSN LISKTSNLDI LVCNAGITSD TLAIRMKDQD FDKVIDINLK ANFILNREAI KKMIQKRYGR IINISSIVGI AGNPGQANYC ASKAGLIGMT KSLSYEVATR GITVNAVAPG FIKSDMTDKL NEKQREAIVQ KIPLGTYGIP EDVAYAVAFL ASNNASYITG QTLHVNGGML MV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattgattta acaggtaaaa cttccttaat tacaggtgct tcaagtggca taggaagtgc catagcgcgg ttactacata aactcgggag caaggtaatt attagcggta gtaatgaaga aaaattaaaa tcacttggca atgctttaaa agataattat accatagaag tatgtaatct tgcgaataag gaagaatgta gtaatttaat atctaaaaca tcaaatttag atattttagt atgtaatgcc ggtattacta gtgatacact cgcaattaga atgaaagatc aagatttcga caaagttatt gatattaatt tgaaagccaa ttttatttta aatcgtgaag caataaaaaa aatgatacaa aaaaggtatg gacgcattat taacatatct tcaatagtag gaattgcagg taacccagga caggctaatt attgcgcttc taaagcaggt ttaataggta tgaccaaatc gctatcttac gaagttgcaa ctagagggat tacggttaat gcagtagcac caggatttat taaatcagat atgactgata aactcaatga aaaacaacga gaagcaatag tacaaaaaat tccactaggt acttatggta taccagaaga cgttgcatac gcagtcgcat ttttagcaag caataatgca tcatatatta ctggtcaaac tctacatgta aacggtggta tgttaatggt aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for RiprA.00010.b.A1.PS00093
| PURIFICATION DATe: | 10/23/2008 |
| CONCENTRATION: | 25.35mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 23 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIDLTGKTS LITGASSGIG SAIARLLHKL GSKVIISGSN EEKLKSLGNA LKDNYTIEVC NLANKEECSN LISKTSNLDI LVCNAGITSD TLAIRMKDQD FDKVIDINLK ANFILNREAI KKMIQKRYGR IINISSIVGI AGNPGQANYC ASKAGLIGMT KSLSYEVATR GITVNAVAPG FIKSDMTDKL NEKQREAIVQ KIPLGTYGIP EDVAYAVAFL ASNNASYITG QTLHVNGGML MV
Validated NT Sequence
taatacgact cactataggg agaccacaan ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgattga tttaacaggt aaaacttcct taattacagg tgcttcaagt ggcataggaa gtgccatagc gcggttacta cataaactcg ggagcaaggt aattattagc ggtagtaatg aagaaaaatt aaaatcactt ggcaatgctt taaaagataa ttataccata gaagtatgta atcttgcgaa taaggaagaa tgtagtaatt taatatctaa aacatcaaat ttagatattt tagtatgtaa tgccggtatt actagtgata cactcgcaat tagaatgaaa gatcaagatt tcgacaaagt tattgatatt aatttgaaag ccaattttat tttaaatcgt gaagcaataa aaaaaatgat acaaaaaagg tatggacgca ttattaacat atcttcaata gtaggaattg caggtaaccc aggacaggct aattattgcg cttctaaagc aggtttaata ggtatgacca aatcgctatc ttacgaagtt gcaactagag ggattacggt taatgcagta gcaccaggat ttattaaatc agatatgact gataaactca atgaaaaaca acgagaagca atagtacaaa aaattccact aggtacttat ggtataccag aagacgttgc atacgcagtc gcatttttag caagcaataa tgcatcatat attactggtc aaactttaca tgtaaacggt ggtatgttaa tggtataata aacagcacga acaagttctg cagccaagct tctcgaggnt ccggctgcta acaaagcccg aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIDLTGKTS LITGASSGIG SAIARLLHKL GSKVIISGSN EEKLKSLGNA LKDNYTIEVC NLANKEECSN LISKTSNLDI LVCNAGITSD TLAIRMKDQD FDKVIDINLK ANFILNREAI KKMIQKRYGR IINISSIVGI AGNPGQANYC ASKAGLIGMT KSLSYEVATR GITVNAVAPG FIKSDMTDKL NEKQREAIVQ KIPLGTYGIP EDVAYAVAFL ASNNASYITG QTLHVNGGML MV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTGATTTA ACAGGTAAAA CTTCCTTAAT TACAGGTGCT TCAAGTGGCA TAGGAAGTGC CATAGCGCGG TTACTACATA AACTCGGGAG CAAGGTAATT ATTAGCGGTA GTAATGAAGA AAAATTAAAA TCACTTGGCA ATGCTTTAAA AGATAATTAT ACCATAGAAG TATGTAATCT TGCGAATAAG GAAGAATGTA GTAATTTAAT ATCTAAAACA TCAAATTTAG ATATTTTAGT ATGTAATGCC GGTATTACTA GTGATACACT CGCAATTAGA ATGAAAGATC AAGATTTCGA CAAAGTTATT GATATTAATT TGAAAGCCAA TTTTATTTTA AATCGTGAAG CAATAAAAAA AATGATACAA AAAAGGTATG GACGCATTAT TAACATATCT TCAATAGTAG GAATTGCAGG TAACCCAGGA CAGGCTAATT ATTGCGCTTC TAAAGCAGGT TTAATAGGTA TGACCAAATC GCTATCTTAC GAAGTTGCAA CTAGAGGGAT TACGGTTAAT GCAGTAGCAC CAGGATTTAT TAAATCAGAT ATGACTGATA AACTCAATGA AAAACAACGA GAAGCAATAG TACAAAAAAT TCCACTAGGT ACTTATGGTA TACCAGAAGA CGTTGCATAC GCAGTCGCAT TTTTAGCAAG CAATAATGCA TCATATATTA CTGGTCAAAC TCTACATGTA AACGGTGGTA TGTTAATGGT AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA