RiprA.00010.a

acetoacetyl-CoA reductase

CENTER ID: RiprA.00010.a
ORGANISM: Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE: Endemic typhus
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiprA.00010.a.A1.PW25644 Full length( RiprA.00010.a ) 1 241

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272947.5.peg.36
RefSeq: NP_220429.1
UniProt: Q9ZEB0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEIAIVTGG TRGIGKATAL ELKNKDLTVI ANFFSNYDAA KEMESKYGIR TKCWNVADLE ECKKAVNEIE EEFKKSVSIL VNNAGITKDK MLHRMSNQDW TDVINVNLHS CFNMSSSVMK QMRNQNYGRI VNISSINAQA GQIGQTNYSA AKAGIIGFTK ALARETASKN ITVNCIAPGY IATEMVNTVP KDILTKIVNS IPKKRLGQPE EIARAVAFLV DKNAGFITGE TISINGGHNM I
NT Sequence
ATGTCAGAAA TTGCAATTGT AACAGGTGGC ACTAGAGGGA TAGGGAAAGC TACTGCTTTA GAATTAAAAA ATAAGGATCT TACGGTAATT GCTAATTTTT TCAGTAATTA TGATGCTGCT AAGGAAATGG AATCAAAATA TGGTATAAGA ACTAAATGCT GGAATGTTGC AGATTTGGAA GAATGTAAAA AAGCAGTAAA TGAAATTGAA GAGGAATTTA AAAAATCAGT AAGTATTCTT GTTAACAATG CAGGTATTAC CAAAGATAAA ATGCTACATA GAATGAGTAA TCAAGATTGG ACCGACGTTA TTAACGTTAA TCTTCATTCT TGCTTTAATA TGTCTAGCAG TGTAATGAAA CAGATGCGCA ACCAAAATTA TGGTCGTATA GTAAATATCA GTTCAATTAA TGCCCAAGCA GGACAGATTG GTCAAACTAA TTACTCTGCT GCTAAAGCTG GGATCATTGG TTTTACTAAG GCTCTTGCAC GTGAAACTGC TTCTAAAAAT ATCACAGTTA ACTGCATAGC GCCAGGATAT ATTGCGACAG AAATGGTAAA TACAGTACCT AAAGATATAC TTACTAAAAT AGTTAATAGT ATTCCTAAAA AAAGACTAGG ACAACCTGAA GAAATAGCTA GAGCAGTAGC ATTTTTAGTT GATAAAAATG CAGGTTTTAT AACAGGCGAG ACTATTTCAA TCAATGGTGG CCATAATATG ATT
Details for RiprA.00010.a.A1.PW25644
PURIFICATION DATe: 4/5/2010
CONCENTRATION: 3.76mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 50µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIAIVTG GTRGIGKATA LELKNKDLTV IANFFSNYDA AKEMESKYGI RTKCWNVADL EECKKAVNEI EEEFKKSVSI LVNNAGITKD KMLHRMSNQD WTDVINVNLH SCFNMSSSVM KQMRNQNYGR IVNISSINAQ AGQIGQTNYS AAKAGIIGFT KALARETASK NITVNCIAPG YIATEMVNTV PKDILTKIVN SIPKKRLGQP EEIARAVAFL VDKNAGFITG ETISINGGHN MX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcag aaattgcaat tgtaacaggt ggcactagag ggatagggaa agctactgct ttagaattaa aaaataagga tcttacggta attgctaatt ttttcagtaa ttatgatgct gctaaggaaa tggaatcaaa atatggtata agaactaaat gctggaatgt tgcagatttg gaagaatgta aaaaagcagt aaatgaaatt gaagaggaat ttaaaaaatc agtaagtatt cttgttaaca atgcaggtat taccaaagat aaaatgctac atagaatgag taatcaagat tggaccgacg ttattaacgt taatcttcat tcttgcttta atatgtctag cagtgtaatg aaacagatgc gcaaccaaaa ttatggtcgt atagtaaata tcagttcaat taatgcccaa gcaggacaga ttggtcaaac taattactct gctgctaaag ctgggatcat tggttttact aaggctcttg cacgtgaaac tgcttctaaa aatatcacag ttaactgcat agcgccagga tatattgcga cagaaatggt aaatacagta cctaaagata tacttactaa aatagttaat agtattccta aaaaaagact aggacaacct gaagaaatag ctagagcagt agcattttta gttgataaaa atgcaggttt tataacaggc gagactattt caatcaatgg tggccataat atganttaat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIAIVTG GTRGIGKATA LELKNKDLTV IANFFSNYDA AKEMESKYGI RTKCWNVADL EECKKAVNEI EEEFKKSVSI LVNNAGITKD KMLHRMSNQD WTDVINVNLH SCFNMSSSVM KQMRNQNYGR IVNISSINAQ AGQIGQTNYS AAKAGIIGFT KALARETASK NITVNCIAPG YIATEMVNTV PKDILTKIVN SIPKKRLGQP EEIARAVAFL VDKNAGFITG ETISINGGHN MI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAGAAATT GCAATTGTAA CAGGTGGCAC TAGAGGGATA GGGAAAGCTA CTGCTTTAGA ATTAAAAAAT AAGGATCTTA CGGTAATTGC TAATTTTTTC AGTAATTATG ATGCTGCTAA GGAAATGGAA TCAAAATATG GTATAAGAAC TAAATGCTGG AATGTTGCAG ATTTGGAAGA ATGTAAAAAA GCAGTAAATG AAATTGAAGA GGAATTTAAA AAATCAGTAA GTATTCTTGT TAACAATGCA GGTATTACCA AAGATAAAAT GCTACATAGA ATGAGTAATC AAGATTGGAC CGACGTTATT AACGTTAATC TTCATTCTTG CTTTAATATG TCTAGCAGTG TAATGAAACA GATGCGCAAC CAAAATTATG GTCGTATAGT AAATATCAGT TCAATTAATG CCCAAGCAGG ACAGATTGGT CAAACTAATT ACTCTGCTGC TAAAGCTGGG ATCATTGGTT TTACTAAGGC TCTTGCACGT GAAACTGCTT CTAAAAATAT CACAGTTAAC TGCATAGCGC CAGGATATAT TGCGACAGAA ATGGTAAATA CAGTACCTAA AGATATACTT ACTAAAATAG TTAATAGTAT TCCTAAAAAA AGACTAGGAC AACCTGAAGA AATAGCTAGA GCAGTAGCAT TTTTAGTTGA TAAAAATGCA GGTTTTATAA CAGGCGAGAC TATTTCAATC AATGGTGGCC ATAATATGAT TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA