RiprA.00002.a

2,3,4,5-TETRAHYDROPYRIDINE-2-CARBOXYLATE N-SUCCINYLTRANSFERASE (dapD)

CENTER ID:	RiprA.00002.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00002.a.A1.GE25257	Full length( RiprA.00002.a )		1	274

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00002.a.A1.PS00082	Full length( RiprA.00002.a )		1	274

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_134244
PATRIC ID:	fig\|272947.5.peg.201
RefSeq:	NP_220583
UniProt:	Q9ZDX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSYIIKEIEE AWQIKENILH DSSKLIKLKK ILNESIASLN QGIIRVCEKQ GNQWKVNEWV KKAILLYFIT TESQLYNNNY NSWYDKVAPK FPADTDKNIF KEAAIRKVPG AIVRTGTYIA KNVVIMPSFI NIGAYIDEGT MIDTWATIGS CAQIGKNCHI SGGAGIGGVL EPLQAKPVII EDNCFVGARS EIAEGVIVEE GSVISMGVFI GSSTKIVYRD TGEIIYGRIP AYSVVVPGIL PPPEVGKPGL YCVVIVKQVD KTTRGKVSIN DLLR

NT Sequence

ATGTCCTATA TTATTAAAGA AATTGAAGAA GCTTGGCAAA TTAAGGAAAA TATTCTTCAT GATTCTTCAA AGCTTATAAA ATTAAAGAAA ATACTTAATG AGAGTATCGC AAGTTTAAAT CAAGGTATAA TTCGTGTTTG TGAAAAGCAA GGCAATCAGT GGAAAGTTAA TGAATGGGTA AAGAAAGCTA TATTGCTATA TTTTATCACT ACAGAATCGC AGCTTTATAA TAACAATTAT AATAGTTGGT ATGATAAAGT TGCTCCTAAA TTTCCTGCAG ATACTGATAA GAATATATTC AAAGAAGCAG CGATACGCAA AGTTCCAGGA GCTATTGTGA GAACTGGTAC TTATATCGCT AAAAATGTTG TAATTATGCC CTCTTTCATT AATATCGGTG CTTATATAGA TGAGGGTACA ATGATTGATA CATGGGCTAC TATTGGATCA TGTGCTCAAA TAGGTAAAAA TTGTCATATT TCCGGTGGTG CAGGAATAGG TGGGGTGCTT GAGCCATTGC AAGCCAAGCC TGTTATAATT GAAGATAATT GCTTTGTTGG AGCAAGATCT GAAATAGCAG AAGGTGTAAT AGTAGAGGAA GGATCAGTAA TTAGTATGGG TGTGTTTATT GGTAGCTCTA CAAAAATAGT ATATAGAGAT ACAGGCGAAA TTATTTATGG TAGAATTCCA GCTTATTCTG TTGTGGTACC AGGGATATTA CCTCCTCCTG AGGTAGGCAA GCCTGGACTT TATTGCGTAG TTATTGTAAA GCAGGTTGAT AAAACTACTC GAGGTAAAGT AAGCATCAAT GATTTATTAA GG

Details for RiprA.00002.a.A1.GE25257

HARVESTED ON:	5/6/2008
SEQUENCED ON:	6/16/2009
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	94

Validated AA Sequence

MGTLEAQTQG PGSMSYIIKE IEEAWQIKEN ILHDSSKLIK LKKILNESIA SLNQGIIRVC EKQGNQWKVN EWVKKAILLY FITTESQLYN NNYNSWYDKV APKFPADTDK NIFKEAAIRK VPGAIVRTGT YIAKNVVIMP SFINIGAYID EGTMIDTWAT IGSCAQIGKN CHISGGAGIG GVLEPLQAKP VIIEDNCFVG ARSEIAEGVI VEEGSVISMG VFIGSSTKIV YRDTGEIIYG RIPAYSVVVP GILPPPEVGK PGLYCVVIVK XLIKLLVVK

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtccta tattattaaa gaaattgaag aagcatggca aattaaggaa aatattcttc atgattcttc aaagctgata aaattaaaga aaatacttaa tgagagtatc gcaagtttaa atcaaggtat aattcgtgtt tgtgaaaagc aaggcaatca gtggaaagtt aatgaatggg taaagaaagc tatattgcta tattttatca ctacagaatc gcagctttat aataacaatt ataatagttg gtatgataaa gttgctccta aatttcctgc agatactgat aagaatatat tcaaagaagc agcgatacgc aaagttccag gagctattgt gagaactggt acttatatcg ctaaaaatgt tgtaattatg ccctctttca ttaatatcgg tgcttatata gatgagggta caatgattga tacatgggct actattggat catgtgctca aataggtaaa aattgtcata tttccggtgg tgcaggaata ggtggggtgc ttgagccatt gcaagccaag cctgttataa ttgaagataa ttgctttgtt ggagcaagat ctgaaatagc agaaggtgta atagtagagg aaggatcagt aattagtatg ggtgtgttta ttggtagctc tacaaaaata gtatatagag atacaggcga aattatttat ggtagaattc cagcttattc tgttgtggta ccagggatat tacctcctcc tgaggtaggc aagcctggac tttattgcgt agttattgta aagcngttga taaaactact cgtggtaaag taagcatcaa tgatttatta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSYIIKEIE EAWQIKENIL HDSSKLIKLK KILNESIASL NQGIIRVCEK QGNQWKVNEW VKKAILLYFI TTESQLYNNN YNSWYDKVAP KFPADTDKNI FKEAAIRKVP GAIVRTGTYI AKNVVIMPSF INIGAYIDEG TMIDTWATIG SCAQIGKNCH ISGGAGIGGV LEPLQAKPVI IEDNCFVGAR SEIAEGVIVE EGSVISMGVF IGSSTKIVYR DTGEIIYGRI PAYSVVVPGI LPPPEVGKPG LYCVVIVKQV DKTTRGKVSI NDLLR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcctatatt attaaagaaa ttgaagaagc atggcaaatt aaggaaaata ttcttcatga ttcttcaaag ctgataaaat taaagaaaat acttaatgag agtatcgcaa gtttaaatca aggtataatt cgtgtttgtg aaaagcaagg caatcagtgg aaagttaatg aatgggtaaa gaaagctata ttgctatatt ttatcactac agaatcgcag ctttataata acaattataa tagttggtat gataaagttg ctcctaaatt tcctgcagat actgataaga atatattcaa agaagcagcg atacgcaaag ttccaggagc tattgtgaga actggtactt atatcgctaa aaatgttgta attatgccct ctttcattaa tatcggtgct tatatagatg agggtacaat gattgataca tgggctacta ttggatcatg tgctcaaata ggtaaaaatt gtcatatttc cggtggtgca ggaataggtg gggtgcttga gccattgcaa gccaagcctg ttataattga agataattgc tttgttggag caagatctga aatagcagaa ggtgtaatag tagaggaagg atcagtaatt agtatgggtg tgtttattgg tagctctaca aaaatagtat atagagatac aggcgaaatt atttatggta gaattccagc ttattctgtt gtggtaccag ggatattacc tcctcctgag gtaggcaagc ctggacttta ttgcgtagtt attgtaaagc aggttgataa aactactcgt ggtaaagtaa gcatcaatga tttattaagg tgaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for RiprA.00002.a.A1.PS00082

PURIFICATION DATe:	10/1/2008
CONCENTRATION:	30.9mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	17
VIAL VOLUME:	50µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCTATATT ATTAAAGAAA TTGAAGAAGC ATGGCAAATT AAGGAAAATA TTCTTCATGA TTCTTCAAAG CTGATAAAAT TAAAGAAAAT ACTTAATGAG AGTATCGCAA GTTTAAATCA AGGTATAATT CGTGTTTGTG AAAAGCAAGG CAATCAGTGG AAAGTTAATG AATGGGTAAA GAAAGCTATA TTGCTATATT TTATCACTAC AGAATCGCAG CTTTATAATA ACAATTATAA TAGTTGGTAT GATAAAGTTG CTCCTAAATT TCCTGCAGAT ACTGATAAGA ATATATTCAA AGAAGCAGCG ATACGCAAAG TTCCAGGAGC TATTGTGAGA ACTGGTACTT ATATCGCTAA AAATGTTGTA ATTATGCCCT CTTTCATTAA TATCGGTGCT TATATAGATG AGGGTACAAT GATTGATACA TGGGCTACTA TTGGATCATG TGCTCAAATA GGTAAAAATT GTCATATTTC CGGTGGTGCA GGAATAGGTG GGGTGCTTGA GCCATTGCAA GCCAAGCCTG TTATAATTGA AGATAATTGC TTTGTTGGAG CAAGATCTGA AATAGCAGAA GGTGTAATAG TAGAGGAAGG ATCAGTAATT AGTATGGGTG TGTTTATTGG TAGCTCTACA AAAATAGTAT ATAGAGATAC AGGCGAAATT ATTTATGGTA GAATTCCAGC TTATTCTGTT GTGGTACCAG GGATATTACC TCCTCCTGAG GTAGGCAAGC CTGGACTTTA TTGCGTAGTT ATTGTAAAGC AGGTTGATAA AACTACTCGT GGTAAAGTAA GCATCAATGA TTTATTAAGG TGAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA