RimaA.01649.a

Hemolysin C homolog

CENTER ID: RimaA.01649.a
ORGANISM: Rickettsia massiliae Mtu5
ASSOCIATED DISEASE: Spotted fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RimaA.01649.a.B1.GE36284 Full length( RimaA.01649.a ) 1 299
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|416276.5.peg.1446
RefSeq: YP_001499704.1
UniProt: A8F2M1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKSSKKEDS SKKNQNNKLI FTVRKLFSPI KNFFRKTKTP DNFFGVIKRL KINSQKMTLD ERNILANLLE LEYKTIEDIM VPRSDIAAIK LTTNLEELSE SIKLEVPHTR TLIYDGTLDN VVGFIHIKDL FKALATKQNG RLKKLIRKHI IAAPSMKLLD LLAKMRRERT HIAIVVDEYG GTDGLVTIED LIEEIVGRID DEHDQQLDSD NFKVINNSTI ISNARVEVEV LEEIIGEKLQ NDDDEFDTIG GLVLTRVSSV PAIGTRIDIS ENIEIEVTDA TPRSLKQVKI RLKNGLNGK
NT Sequence
ATGTTAAAAT CTTCAAAAAA AGAAGATTCT AGTAAAAAAA ATCAAAATAA TAAGTTAATT TTTACTGTAC GCAAATTATT CTCTCCGATA AAAAATTTTT TTAGAAAAAC AAAAACGCCC GATAATTTTT TTGGTGTCAT AAAACGCCTT AAAATTAATA GCCAAAAAAT GACTTTAGAC GAGCGTAATA TTTTAGCTAA TTTATTGGAG CTAGAATATA AAACTATTGA AGATATAATG GTACCGCGTT CCGATATTGC GGCAATAAAA TTAACTACGA ATCTAGAAGA ATTAAGTGAG TCTATTAAGT TAGAAGTCCC ACACACACGA ACTCTTATAT ATGACGGTAC TTTGGATAAT GTAGTAGGGT TCATTCATAT CAAGGATTTA TTTAAAGCAT TGGCTACAAA GCAAAACGGT CGTTTAAAAA AGCTTATACG TAAGCATATA ATTGCAGCTC CTTCTATGAA ATTATTAGAT TTGCTAGCAA AAATGCGTCG TGAGAGAACA CATATTGCAA TAGTTGTTGA TGAGTATGGC GGTACTGACG GTTTAGTTAC TATTGAAGAT CTTATAGAAG AAATAGTAGG ACGAATAGAT GATGAGCATG ATCAGCAATT AGATAGCGAT AATTTCAAGG TTATTAATAA CTCAACAATT ATTTCAAATG CACGTGTTGA AGTAGAAGTG CTTGAAGAAA TAATAGGAGA AAAGCTACAA AATGATGATG ATGAGTTTGA TACGATAGGT GGATTAGTAT TGACTAGAGT CAGTAGTGTT CCGGCTATCG GTACTAGAAT AGATATTTCA GAAAATATTG AAATTGAAGT TACGGACGCA ACTCCTCGTT CTTTAAAACA AGTCAAAATT AGACTAAAAA ACGGTTTGAA TGGTAAA
Details for RimaA.01649.a.B1.GE36284
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 35kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML KSSKKEDSSK KNQNNKLIFT VRKLFSPIKN FFRKTKTPDN FFGVIKRLKI NSQKMTLDER NILANLLELE DKTIEDIMVP RSDIAAIKLT TNLEELSESI KLEVPHTRTL IYDGTLDNVV GFIHIKDLFK ALATKQNGRL KKLIRKHIIA APSMKLLDLL AKMRRERTHI AIVVDEYGGT DGLVTIEDLI EEIVGRIDDE HDQQLDSDNF KVINNSTIIS NARVEVEVLE EIIGEKLHND YDEFDTIGGL VLTRVSSVPA IGTRIDISEN IEIEVTDATP RSLKQVKIRL KNGLNGK
Validated NT Sequence
tcttactcac ttatttacca ttcaaaccgt tttttagtct aattttgact tgttttaaag aacgaggagt tgcgtccgta acttcaattt caatattttc tgaaatatct attctagtac cgatagccgg aacactactg actctagtca atactaatcc acctatcgta tcaaactcat cataatcatt gtgtagcttt tctcctatta tttcttctag cacttctact tcaacacgtg catttgaaat aattgttgag ttattaataa ccttgaaatt atcgctatct aattgctgat catgctcatc atctattcgt cctactattt cttctataag atcttcaata gtaactaaac cgtcagtacc gccatactca tcaacaacta ttgcaatatg tgttctctca cgacgcattt ttgctagcaa atctaataat ttcatagaag gagctgcaat tatatgctta cgtataagct tttttaaacg accattttgc tttgtagcca atgctttaaa taaatccttg atatgaatga accctactac attatccaaa gtaccatcat atataagagt tcgtgtatgt gggacttcta acttaataga ctcacttaat tcttctagat tcgtagttaa ttttattgcc gcaatatcgg aacgcggtac cattatatct tcaatagttt tatcttctag ctccaataaa ttagctaaaa tattacgctc gtctaaagtc attttttggc tattaatttt aaggcgtttt atgacaccaa aaaaattatc gggcgttttt gtttttctaa aaaaattttt tatcggagag aataattttc gtacagtaaa aattaactta ttattttgat tttttttact agaatcttct ttttttgaag attttaacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHML KSSKKEDSSK KNQNNKLIFT VRKLFSPIKN FFRKTKTPDN FFGVIKRLKI NSQKMTLDER NILANLLELE YKTIEDIMVP RSDIAAIKLT TNLEELSESI KLEVPHTRTL IYDGTLDNVV GFIHIKDLFK ALATKQNGRL KKLIRKHIIA APSMKLLDLL AKMRRERTHI AIVVDEYGGT DGLVTIEDLI EEIVGRIDDE HDQQLDSDNF KVINNSTIIS NARVEVEVLE EIIGEKLQND DDEFDTIGGL VLTRVSSVPA IGTRIDISEN IEIEVTDATP RSLKQVKIRL KNGLNGK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa aatcttcaaa aaaagaagat tctagtaaaa aaaatcaaaa taataagtta atttttactg tacgcaaatt attctctccg ataaaaaatt tttttagaaa aacaaaaacg cccgataatt tttttggtgt cataaaacgc cttaaaatta atagccaaaa aatgacttta gacgagcgta atattttagc taatttattg gagctagaat ataaaactat tgaagatata atggtaccgc gttccgatat tgcggcaata aaattaacta cgaatctaga agaattaagt gagtctatta agttagaagt cccacacaca cgaactctta tatatgacgg tactttggat aatgtagtag ggttcattca tatcaaggat ttatttaaag cattggctac aaagcaaaac ggtcgtttaa aaaagcttat acgtaagcat ataattgcag ctccttctat gaaattatta gatttgctag caaaaatgcg tcgtgagaga acacatattg caatagttgt tgatgagtat ggcggtactg acggtttagt tactattgaa gatcttatag aagaaatagt aggacgaata gatgatgagc atgatcagca attagatagc gataatttca aggttattaa taactcaaca attatttcaa atgcacgtgt tgaagtagaa gtgcttgaag aaataatagg agaaaagcta caaaatgatg atgatgagtt tgatacgata ggtggattag tattgactag agtcagtagt gttccggcta tcggtactag aatagatatt tcagaaaata ttgaaattga agttacggac gcaactcctc gttctttaaa acaagtcaaa attagactaa aaaacggttt gaatggtaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat