SSGCID: Target Page

RifeA.18411.a

VirB10 protein

CENTER ID:	RifeA.18411.a
ORGANISM:	Rickettsia felis URRWXCal2
ASSOCIATED DISEASE:	Flea-borne spotted fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.18411.a.B2.GE39997	soluble domain		57	481
RifeA.18411.a.B3.GE39998	globular domain (aln H.pyl struc)		189	481

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.18411.a.B3.PS38210	globular domain (aln H.pyl struc)		189	481

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|315456.7.peg.550
RefSeq:	WP_011270799.1
UniProt:	Q4UM99

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEEQNNNDT GSLSGADAPE VQRELSKVSV SFNKSIAIVV VICGIFIYIF YTLFFANKKE EISETQVPTN IVKPVTDVDD NIPEIPKLPD PPKLETPTAP PPPPPPVVEV PPVLPPTTPV EENKDKTLPL PPVSLPSTSG TLVESDAEKQ RREAKRKSAI VLVGGVEPKK TQEQITTEAT FKDRGDMSLV LGRGKLIDAV LETAINSDLG GEIRAIISRD VFSEKDKVIL IPKGSKIFGK YATSTDSSSY GRVSIIWDRI DLTNGYTIEF DSPAVDNLGR PGLQGRVDNK YKEQFANAVL QSGFNIGLAK VLDKLVPPPI DSQAAATNSA TATQLLNTAQ TIAANTAMDA NTRIVTICTN ILAAITDKTS TAYTTMTQAC TTAQTASSAN TAEQRLQTLV QAVNTAASSL LTTTSIASTP TQAQQASTQA FTDVTNVVQN MITQQPFKPT TTVNQGTPVR IYVNKDYKFP KAVLLKSKVM K

NT Sequence

atggctgaag agcaaaataa taatgatacc ggttctttat caggagcaga tgctcctgaa gtacagagag aattatcaaa agtttcagtt agctttaata agagtattgc gatagtagtc gtaatttgcg gtatttttat atatattttt tatactcttt ttttcgctaa taaaaaagaa gaaatatcgg agactcaagt gccgactaat attgtaaaac ctgttactga tgttgatgat aatattccgg agattccaaa gttacctgat ccgcctaagc ttgagacgcc tactgctcct ccaccacctc ctcctccagt agtagaagtt ccgccggtct tacctcctac tactcctgta gaagagaata aagacaaaac gcttccattg ccgccggttt cgttgccttc gacttcagga acgttagttg agagtgatgc agagaagcaa cgtcgtgaag caaaaagaaa atcagctata gtgttagttg gtggtgtaga gcctaaaaaa acacaggaac agattacgac agaagcgacc tttaaagatc gtggcgatat gtctttggtt cttggacgcg gtaaattaat tgatgcagta cttgaaacgg caataaatag tgatctcggc ggtgaaataa gagccattat tagtagagac gtattttctg agaaagataa agttatattg ataccgaaag gatcaaaaat attcggtaaa tatgcaacct caactgattc gagcagttat ggtagagtat ctataatatg ggatagaata gatttgacca acggttatac tatagaattt gactctcctg cagttgataa tttaggtaga cctggactac aagggagagt cgataataaa tataaagaac aatttgcaaa tgccgtatta caatccggct ttaatatagg ccttgctaaa gttcttgata agttagtgcc gcctcctata gattcacagg cagcagctac caatagtgct acggcaacac aattgttaaa tactgctcaa actattgcgg ctaataccgc tatggatgca aatactagga tagttacaat ttgtactaat atacttgctg ctattaccga taaaacttcg actgcttata ctactatgac tcaagcatgt acaacagcac aaactgcttc ttcagcgaat actgctgagc agagacttca aaccttagta caggcagtta atacagcagc ttcaagtttg cttactacta cctctatagc atcgactccg acacaagcac aacaagcttc tactcaagct tttacggatg tgacgaatgt tgtacaaaat atgataactc agcagccgtt taagccgacg acaacggtta atcaaggtac gccggttaga atatacgtta ataaggatta taaattccca aaagccgttt tattaaaatc aaaggtaatg aaatga

Details for RifeA.18411.a.B2.GE39997

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/1/2016
EXPECTED MW:	47kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMN KKEEISETQV PTNIVKPVTD VDDNIPEIPK LPDPPKLETP TAPPPPPPPV VEVPPVLPPT TPVEENKDKT LPLPPVSLPS TSGTLVESDA EKQRREAKRK SAIVLVGGVE PKKTQEQITT EATFKDRGDM SLVLGRGKLI DAVLETAINS DLGGEIRAII SRDVFSEKDK VILIPKGSKI FGKYATSTDS SSYGRVSIIW DRIDLTNGYT IEFDSPAVDN LGRPGLQGRV DNKYKEQFAN AVLQSGFNIG LAKVLDKLVP PPIDSQAAAT NSATATQLLN TAQTIAANTA MDANTRIVTI CTNILAAITD KTSTAYTTMT QACTTAQTAS SANTAEQRLQ TLVQAVNTAA SSLLTTTSIA STPTQAQQAS TQAFTDVTNV VQNMITQQPF KPTTTVNQGT PVRIYVNKDY KFPKAV

Validated NT Sequence

taaaacggct tttgggaatt tataatcctt attaacgtat attctaaccg gcgtaccttg attaaccgtt gtcgtcggct taaacggctg ctgagttatc atattttgta caacattcgt cacatccgta aaagcttgag tagaagcttg ttgtgcttgt gtcggagtcg atgctataga ggtagtagta agcaaacttg aagctgctgt attaactgcc tgtactaagg tttgaagtct ctgctcagca gtattcgctg aagaagcagt ttgtgctgtt gtacatgctt gagtcatagt agtataagca gtcgaagttt tatcggtaat agcagcaagt atattagtac aaattgtaac tatcctagta tttgcatcca tagcggtatt agccgcaata gtttgagcag tatttaacaa ttgtgttgcc gtagcactat tggtagctgc tgcctgtgaa tctataggag gcggcactaa cttatcaaga actttagcaa ggcctatatt aaagccggat tgtaatacgg catttgcaaa ttgttcttta tatttattat cgactctccc ttgtagtcca ggtctaccta aattatcaac tgcaggagag tcaaattcta tagtataacc gttggtcaaa tctattctat cccatattat agatactcta ccataactgc tcgaatcagt tgaggttgca tatttaccga atatttttga tcctttcggt atcaatataa ctttatcttt ctcagaaaat acgtctctac taataatggc tcttatttca ccgccgagat cactatttat tgccgtttca agtactgcat caattaattt accgcgtcca agaaccaaag acatatcgcc acgatcttta aaggtcgctt ctgtcgtaat ctgttcctgt gtttttttag gctctacacc accaactaac actatagctg attttctttt tgcttcacga cgttgcttct ctgcatcact ctcaactaac gttcctgaag tcgaaggcaa cgaaaccggc ggcaatggaa gcgttttgtc tttattctct tctacaggag tagtaggagg taagaccggc ggaacttcta ctactggagg aggaggtggt ggaggagcag taggcgtctc aagcttaggc ggatcaggta actttggaat ctccggaata ttatcatcaa catcagtaac aggttttaca atattagtcg gcacttgagt ctccgatatt tcttcttttt tattcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHNK KEEISETQVP TNIVKPVTDV DDNIPEIPKL PDPPKLETPT APPPPPPPVV EVPPVLPPTT PVEENKDKTL PLPPVSLPST SGTLVESDAE KQRREAKRKS AIVLVGGVEP KKTQEQITTE ATFKDRGDMS LVLGRGKLID AVLETAINSD LGGEIRAIIS RDVFSEKDKV ILIPKGSKIF GKYATSTDSS SYGRVSIIWD RIDLTNGYTI EFDSPAVDNL GRPGLQGRVD NKYKEQFANA VLQSGFNIGL AKVLDKLVPP PIDSQAAATN SATATQLLNT AQTIAANTAM DANTRIVTIC TNILAAITDK TSTAYTTMTQ ACTTAQTASS ANTAEQRLQT LVQAVNTAAS SLLTTTSIAS TPTQAQQAST QAFTDVTNVV QNMITQQPFK PTTTVNQGTP VRIYVNKDYK FPKAVLLKSK VMK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataataaaa aagaagaaat atcggagact caagtgccga ctaatattgt aaaacctgtt actgatgttg atgataatat tccggagatt ccaaagttac ctgatccgcc taagcttgag acgcctactg ctcctccacc acctcctcct ccagtagtag aagttccgcc ggtcttacct cctactactc ctgtagaaga gaataaagac aaaacgcttc cattgccgcc ggtttcgttg ccttcgactt caggaacgtt agttgagagt gatgcagaga agcaacgtcg tgaagcaaaa agaaaatcag ctatagtgtt agttggtggt gtagagccta aaaaaacaca ggaacagatt acgacagaag cgacctttaa agatcgtggc gatatgtctt tggttcttgg acgcggtaaa ttaattgatg cagtacttga aacggcaata aatagtgatc tcggcggtga aataagagcc attattagta gagacgtatt ttctgagaaa gataaagtta tattgatacc gaaaggatca aaaatattcg gtaaatatgc aacctcaact gattcgagca gttatggtag agtatctata atatgggata gaatagattt gaccaacggt tatactatag aatttgactc tcctgcagtt gataatttag gtagacctgg actacaaggg agagtcgata ataaatataa agaacaattt gcaaatgccg tattacaatc cggctttaat ataggccttg ctaaagttct tgataagtta gtgccgcctc ctatagattc acaggcagca gctaccaata gtgctacggc aacacaattg ttaaatactg ctcaaactat tgcggctaat accgctatgg atgcaaatac taggatagtt acaatttgta ctaatatact tgctgctatt accgataaaa cttcgactgc ttatactact atgactcaag catgtacaac agcacaaact gcttcttcag cgaatactgc tgagcagaga cttcaaacct tagtacaggc agttaataca gcagcttcaa gtttgcttac tactacctct atagcatcga ctccgacaca agcacaacaa gcttctactc aagcttttac ggatgtgacg aatgttgtac aaaatatgat aactcagcag ccgtttaagc cgacgacaac ggttaatcaa ggtacgccgg ttagaatata cgttaataag gattataaat tcccaaaagc cgttttatta aaatcaaagg taatgaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for RifeA.18411.a.B3.GE39998

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	32kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHML VLGRGKLIDA VLETAINSDL GGEIRAIISR DVFSEKDKVI LIPKGSKIFG KYATSTDSSS YGRVSIIWDR IDLTNGYTIE FDSPAVDNLG RPGLQGRVDN KYKEQFANAV LQSGFNIGLA KVLDKLVPPP IDSQAAATNS ATATQLLNTA QTIAANTAMD ANTRIVTICT NILAAITDKT STAYTTMTQA CTTAQTASSA NTAEQRLQTL VQAVNTAASS LLTTTSIAST PTQAQQASTQ AFTDVTNVVQ NMITQQPFKP TTTVNQGTPV RIYVNKDYKF PKAXXXKIKG NEISE

Validated NT Sequence

tcttactcac ttatttcatt acctttgatt ttaannnnnn nggcttttgg gaatttataa tccttattaa cgtatattct aaccggcgta ccttgattaa ccgttgtcgt cggcttaaac ggctgctgag ttatcatatt ttgtacaaca ttcgtcacat ccgtaaaagc ttgagtagaa gcttgttgtg cttgtgtcgg agtcgatgct atagaggtag tagtaagcaa acttgaagct gctgtattaa ctgcctgtac taaggtttga agtctctgct cagcagtatt cgctgaagaa gcagtttgtg ctgttgtaca tgcttgagtc atagtagtat aagcagtcga agttttatcg gtaatagcag caagtatatt agtacaaatt gtaactatcc tagtatttgc atccatagcg gtattagccg caatagtttg agcagtattt aacaattgtg ttgccgtagc actattggta gctgctgcct gtgaatctat aggaggcggc actaacttat caagaacttt agcaaggcct atattaaagc cggattgtaa tacggcattt gcaaattgtt ctttatattt attatcgact ctcccttgta gtccaggtct acctaaatta tcaactgcag gagagtcaaa ttctatagta taaccgttgg tcaaatctat tctatcccat attatagata ctctaccata actgctcgaa tcagttgagg ttgcatattt accgaatatt tttgatcctt tcggtatcaa tataacttta tctttctcag aaaatacgtc tctactaata atggctctta tttcaccgcc gagatcacta tttattgccg tttcaagtac tgcatcaatt aatttaccgc gtccaagaac caacatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHLV LGRGKLIDAV LETAINSDLG GEIRAIISRD VFSEKDKVIL IPKGSKIFGK YATSTDSSSY GRVSIIWDRI DLTNGYTIEF DSPAVDNLGR PGLQGRVDNK YKEQFANAVL QSGFNIGLAK VLDKLVPPPI DSQAAATNSA TATQLLNTAQ TIAANTAMDA NTRIVTICTN ILAAITDKTS TAYTTMTQAC TTAQTASSAN TAEQRLQTLV QAVNTAASSL LTTTSIASTP TQAQQASTQA FTDVTNVVQN MITQQPFKPT TTVNQGTPVR IYVNKDYKFP KAVLLKSKVM K

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggttc ttggacgcgg taaattaatt gatgcagtac ttgaaacggc aataaatagt gatctcggcg gtgaaataag agccattatt agtagagacg tattttctga gaaagataaa gttatattga taccgaaagg atcaaaaata ttcggtaaat atgcaacctc aactgattcg agcagttatg gtagagtatc tataatatgg gatagaatag atttgaccaa cggttatact atagaatttg actctcctgc agttgataat ttaggtagac ctggactaca agggagagtc gataataaat ataaagaaca atttgcaaat gccgtattac aatccggctt taatataggc cttgctaaag ttcttgataa gttagtgccg cctcctatag attcacaggc agcagctacc aatagtgcta cggcaacaca attgttaaat actgctcaaa ctattgcggc taataccgct atggatgcaa atactaggat agttacaatt tgtactaata tacttgctgc tattaccgat aaaacttcga ctgcttatac tactatgact caagcatgta caacagcaca aactgcttct tcagcgaata ctgctgagca gagacttcaa accttagtac aggcagttaa tacagcagct tcaagtttgc ttactactac ctctatagca tcgactccga cacaagcaca acaagcttct actcaagctt ttacggatgt gacgaatgtt gtacaaaata tgataactca gcagccgttt aagccgacga caacggttaa tcaaggtacg ccggttagaa tatacgttaa taaggattat aaattcccaa aagccgtttt attaaaatca aaggtaatga aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for RifeA.18411.a.B3.PS38210

PURIFICATION DATe:	2/23/2017
CONCENTRATION:	15.9mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	101

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGTTC TTGGACGCGG TAAATTAATT GATGCAGTAC TTGAAACGGC AATAAATAGT GATCTCGGCG GTGAAATAAG AGCCATTATT AGTAGAGACG TATTTTCTGA GAAAGATAAA GTTATATTGA TACCGAAAGG ATCAAAAATA TTCGGTAAAT ATGCAACCTC AACTGATTCG AGCAGTTATG GTAGAGTATC TATAATATGG GATAGAATAG ATTTGACCAA CGGTTATACT ATAGAATTTG ACTCTCCTGC AGTTGATAAT TTAGGTAGAC CTGGACTACA AGGGAGAGTC GATAATAAAT ATAAAGAACA ATTTGCAAAT GCCGTATTAC AATCCGGCTT TAATATAGGC CTTGCTAAAG TTCTTGATAA GTTAGTGCCG CCTCCTATAG ATTCACAGGC AGCAGCTACC AATAGTGCTA CGGCAACACA ATTGTTAAAT ACTGCTCAAA CTATTGCGGC TAATACCGCT ATGGATGCAA ATACTAGGAT AGTTACAATT TGTACTAATA TACTTGCTGC TATTACCGAT AAAACTTCGA CTGCTTATAC TACTATGACT CAAGCATGTA CAACAGCACA AACTGCTTCT TCAGCGAATA CTGCTGAGCA GAGACTTCAA ACCTTAGTAC AGGCAGTTAA TACAGCAGCT TCAAGTTTGC TTACTACTAC CTCTATAGCA TCGACTCCGA CACAAGCACA ACAAGCTTCT ACTCAAGCTT TTACGGATGT GACGAATGTT GTACAAAATA TGATAACTCA GCAGCCGTTT AAGCCGACGA CAACGGTTAA TCAAGGTACG CCGGTTAGAA TATACGTTAA TAAGGATTAT AAATTCCCAA AAGCCGTTTT ATTAAAATCA AAGGTAATGA AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT