SSGCID: Target Page

RifeA.17987.a

DNA polymerase III subunit beta (EC 2.7.7.7)

CENTER ID:	RifeA.17987.a
ORGANISM:	Rickettsia felis URRWXCal2
ASSOCIATED DISEASE:	Flea-borne spotted fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.17987.a.B1.GE41039	full length		1	379

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.17987.a.B1.PW38224	full length		1	379

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|315456.7.peg.776
UniProt:	Q4ULS3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKLIVETKT LVQSLGFASS VVEKRNVIPE YANIKLSAKD GNLELSSTNM DLYLSQKIAV QVVSEGELTV STKTLNDIVR KLPDSELTLT DLGTTGLEIK GKNCKFNLFT LPVSSFPPMD SINPEASFKI SCTDFAKIIE STKFSISLDE TRYNLNGVYL HIKDKEFCSA STDGHRLSIS WVTLEKQIKN FGVILPQKSA EEILKIVKDP KNINEDIEIL LSSNKIKFIC NENTIMLSKL IDGTFPDYSA FIPESSSSKL VINRKIFADS IERIAIITVE KFRAVKLSLS RETLEISAVG EARGNAKEVI NSSQDKESFY EYNSDESLAI GFNPQYLEDV LKAVKSDLVE LYFSDVSAPV LIKFPENPKD IFVVMPVKV

NT Sequence

atgttaaaat taatagttga aacaaaaacg ttagttcagt ccctagggtt tgcaagttcc gttgttgaaa aacgtaatgt aatacctgaa tatgcaaaca ttaaattatc ggcaaaagac ggcaatctag agcttagttc tactaatatg gatttgtact taagtcaaaa aatagcagta caggtagtaa gtgaaggtga acttaccgtt tctacgaaaa ctctaaatga tatagtccga aaacttccgg attccgagct tacattaaca gatctcggta caacgggact tgaaatcaaa ggaaaaaatt gtaaatttaa tttatttact ttgccggtta gttcttttcc tccaatggat agtattaatc ctgaagcaag ttttaaaatt tcctgcacgg attttgctaa aataatcgaa tcgacaaaat tttcgatttc tttagatgaa actcgttata atttaaacgg tgtttattta cacataaaag acaaagagtt ttgttcggca agtaccgatg gacataggct ttcgatttca tgggtaacat tagaaaaaca aataaagaat tttggagtta tattaccgca aaaaagtgcc gaggaaattt taaaaatagt taaagatcct aaaaatataa atgaagatat agaaatttta ttaagtagca ataagattaa atttatatgt aacgaaaata ctattatgct atcaaagctt atagacggta ctttccctga ttatagtgct tttattccgg aaagcagcag ctcaaaatta gtaattaatc gaaaaatatt tgcagatagt atagaaagga tagcgataat aaccgttgaa aaatttagag cggtaaaatt atccttatct cgtgaaacct tagagattag tgccgtcggt gaggcaagag gtaacgcaaa agaggttatt aattcttcac aagataagga aagtttttat gaatataata gtgatgagtc tttagctata ggctttaatc cgcaatattt ggaagatgtc ttaaaagccg tgaagtcgga tttagtagaa ctatattttt cagatgtttc agcaccggta cttattaaat ttcctgagaa tcctaaagat atttttgtcg tcatgccagt taaggtgtag

Details for RifeA.17987.a.B1.GE41039

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHML KLIVETKTLV QSLGFASSVV EKRNVIPEYA NIKLSAKDGN LELSSTNMDL YLSQKIAVQV VSEGELTVST KTLNDIVRKL PDSELTLTDL GTTGLEIKGK NCKFNLFTLP VSSFPPMDSI NPEASFKISC TDFAKIIEST KFSISLDETR YNLNGVYLHI KDKEFCSAST DGHRLSISWV TLEKQIKNFG VILPQKSAEE ILKIVKDPKN INEDIEILLS SNKIKFICNE NTIMLSKLID GTFPDYSAFI PESSSSKLVI NRKIFADSIE RIAIITVEKF RAVKLSLSRE TLEISAVGEA RGNAKEVINS SQDKESFYEY NSDESLAIGF NPQYLEDVLK AVKSDLVELY FSDVSAPVLI KFPENPKD

Validated NT Sequence

aatatcttta ggattctcag gaaatttaat aagtaccggt gctgaaacat ctgaaaaata tagttctact aaatccgact tcacggcttt taagacatct tccaaatatt gcggattaaa gcctatagct aaagactcat cactattata ttcataaaaa ctttccttat cttgtgaaga attaataacc tcttttgcgt tacctcttgc ctcaccgacg gcactaatct ctaaggtttc acgagataag gataatttta ccgctctaaa tttttcaacg gttattatcg ctatcctttc tatactatct gcaaatattt ttcgattaat tactaatttt gagctgctgc tttccggaat aaaagcacta taatcaggga aagtaccgtc tataagcttt gatagcataa tagtattttc gttacatata aatttaatct tattgctact taataaaatt tctatatctt catttatatt tttaggatct ttaactattt ttaaaatttc ctcggcactt ttttgcggta atataactcc aaaattcttt atttgttttt ctaatgttac ccatgaaatc gaaagcctat gtccatcggt acttgccgaa caaaactctt tgtcttttat gtgtaaataa acaccgttta aattataacg agtttcatct aaagaaatcg aaaattttgt cgattcgatt attttagcaa aatccgtgca ggaaatttta aaacttgctt caggattaat actatccatt ggaggaaaag aactaaccgg caaagtaaat aaattaaatt tacaattttt tcctttgatt tcaagtcccg ttgtaccgag atctgttaat gtaagctcgg aatccggaag ttttcggact atatcattta gagttttcgt agaaacggta agttcacctt cacttactac ctgtactgct attttttgac ttaagtacaa atccatatta gtagaactaa gctctagatt gccgtctttt gccgataatt taatgtttgc atattcaggt attacattac gtttttcaac aacggaactt gcaaacccta gggactgaac taacgttttt gtttcaacta ttaattttaa catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa aattaatagt tgaaacaaaa acgttagttc agtccctagg gtttgcaagt tccgttgttg aaaaacgtaa tgtaatacct gaatatgcaa acattaaatt atcggcaaaa gacggcaatc tagagcttag ttctactaat atggatttgt acttaagtca aaaaatagca gtacaggtag taagtgaagg tgaacttacc gtttctacga aaactctaaa tgatatagtc cgaaaacttc cggattccga gcttacatta acagatctcg gtacaacggg acttgaaatc aaaggaaaaa attgtaaatt taatttattt actttgccgg ttagttcttt tcctccaatg gatagtatta atcctgaagc aagttttaaa atttcctgca cggattttgc taaaataatc gaatcgacaa aattttcgat ttctttagat gaaactcgtt ataatttaaa cggtgtttat ttacacataa aagacaaaga gttttgttcg gcaagtaccg atggacatag gctttcgatt tcatgggtaa cattagaaaa acaaataaag aattttggag ttatattacc gcaaaaaagt gccgaggaaa ttttaaaaat agttaaagat cctaaaaata taaatgaaga tatagaaatt ttattaagta gcaataagat taaatttata tgtaacgaaa atactattat gctatcaaag cttatagacg gtactttccc tgattatagt gcttttattc cggaaagcag cagctcaaaa ttagtaatta atcgaaaaat atttgcagat agtatagaaa ggatagcgat aataaccgtt gaaaaattta gagcggtaaa attatcctta tctcgtgaaa ccttagagat tagtgccgtc ggtgaggcaa gaggtaacgc aaaagaggtt attaattctt cacaagataa ggaaagtttt tatgaatata atagtgatga gtctttagct ataggcttta atccgcaata tttggaagat gtcttaaaag ccgtgaagtc ggatttagta gaactatatt tttcagatgt ttcagcaccg gtacttatta aatttcctga gaatcctaaa gatatttttg tcgtcatgcc agttaaggtg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for RifeA.17987.a.B1.PW38224

PURIFICATION DATe:	3/13/2017
CONCENTRATION:	37mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAA AATTAATAGT TGAAACAAAA ACGTTAGTTC AGTCCCTAGG GTTTGCAAGT TCCGTTGTTG AAAAACGTAA TGTAATACCT GAATATGCAA ACATTAAATT ATCGGCAAAA GACGGCAATC TAGAGCTTAG TTCTACTAAT ATGGATTTGT ACTTAAGTCA AAAAATAGCA GTACAGGTAG TAAGTGAAGG TGAACTTACC GTTTCTACGA AAACTCTAAA TGATATAGTC CGAAAACTTC CGGATTCCGA GCTTACATTA ACAGATCTCG GTACAACGGG ACTTGAAATC AAAGGAAAAA ATTGTAAATT TAATTTATTT ACTTTGCCGG TTAGTTCTTT TCCTCCAATG GATAGTATTA ATCCTGAAGC AAGTTTTAAA ATTTCCTGCA CGGATTTTGC TAAAATAATC GAATCGACAA AATTTTCGAT TTCTTTAGAT GAAACTCGTT ATAATTTAAA CGGTGTTTAT TTACACATAA AAGACAAAGA GTTTTGTTCG GCAAGTACCG ATGGACATAG GCTTTCGATT TCATGGGTAA CATTAGAAAA ACAAATAAAG AATTTTGGAG TTATATTACC GCAAAAAAGT GCCGAGGAAA TTTTAAAAAT AGTTAAAGAT CCTAAAAATA TAAATGAAGA TATAGAAATT TTATTAAGTA GCAATAAGAT TAAATTTATA TGTAACGAAA ATACTATTAT GCTATCAAAG CTTATAGACG GTACTTTCCC TGATTATAGT GCTTTTATTC CGGAAAGCAG CAGCTCAAAA TTAGTAATTA ATCGAAAAAT ATTTGCAGAT AGTATAGAAA GGATAGCGAT AATAACCGTT GAAAAATTTA GAGCGGTAAA ATTATCCTTA TCTCGTGAAA CCTTAGAGAT TAGTGCCGTC GGTGAGGCAA GAGGTAACGC AAAAGAGGTT ATTAATTCTT CACAAGATAA GGAAAGTTTT TATGAATATA ATAGTGATGA GTCTTTAGCT ATAGGCTTTA ATCCGCAATA TTTGGAAGAT GTCTTAAAAG CCGTGAAGTC GGATTTAGTA GAACTATATT TTTCAGATGT TTCAGCACCG GTACTTATTA AATTTCCTGA GAATCCTAAA GATATTTTTG TCGTCATGCC AGTTAAGGTG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT