SSGCID: Target Page

RifeA.17851.a

Predicted esterase of the alpha-beta hydrolase superfamily

CENTER ID:	RifeA.17851.a
ORGANISM:	Rickettsia felis URRWXCal2
ASSOCIATED DISEASE:	Flea-borne spotted fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.17851.a.B1.GE36163	Full length( RifeA.17851.a )		1	370

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|315456.7.peg.897
RefSeq:	YP_246759.1
UniProt:	Q4ULH9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQYKDLVITA TQQETSELTI FNSFDTPNIE IALACRASAS IPLVFEPVEI DGKKYVDGGY RDNIPTKYFK GNEPEFDTKE VTDNMEKITL AKKQGRTLAM AFGTGMEADA NIAIYSAKKF ESPSDIVKFL ADVLFKTLAK VGGKFKYTET LRETNEQLRE NALNTVVLDT AGIDTLDFKD AQKYSDYLNI KGYCQTREHL NNHDLGKEAD KTFGTKLLEM FISSKENNAS KWQDGIIENH DDKAKMLLSF CKTGALNEKE LNEKLKEYVI IAATSRNNTL KADTNSLKAL LYTLNDPAAS SKIKDSFIEV LGIDKNKDVR FDKTKTFDEN IAKFKFTKQD LESFITKSKS EAPKIQSKYS VARSHVSGGD

NT Sequence

GTGCAGTATA AGGATTTAGT AATTACAGCA ACTCAGCAAG AGACAAGCGA ACTAACTATT TTCAATAGCT TTGATACGCC AAATATTGAA ATTGCTTTAG CTTGCCGTGC TTCTGCTTCT ATACCGCTTG TTTTTGAACC GGTAGAAATT GACGGTAAAA AATATGTCGA CGGCGGATAT AGAGATAATA TACCGACAAA ATATTTTAAA GGCAATGAAC CGGAATTTGA TACAAAAGAA GTAACTGATA ATATGGAAAA GATTACCTTA GCTAAAAAGC AAGGTAGAAC TCTAGCTATG GCTTTTGGCA CGGGTATGGA AGCTGATGCA AATATTGCTA TATATAGTGC AAAAAAATTT GAAAGCCCAA GTGATATCGT AAAGTTTTTA GCTGATGTAT TATTTAAGAC TCTTGCAAAA GTAGGAGGAA AATTTAAATA TACCGAAACT CTAAGAGAAA CTAATGAGCA GTTACGTGAA AATGCTTTAA ACACTGTTGT TTTAGATACT GCCGGAATAG ATACTTTGGA TTTCAAAGAT GCACAAAAAT ATTCTGATTA TTTGAATATT AAAGGATATT GTCAAACTAG AGAACATTTG AATAATCATG ACCTTGGCAA AGAAGCAGAT AAAACATTTG GAACTAAATT ATTAGAAATG TTTATTTCGT CAAAAGAAAA TAATGCTTCC AAATGGCAAG ACGGAATTAT CGAAAATCAT GACGATAAAG CAAAAATGCT ATTATCATTT TGTAAAACCG GAGCATTAAA CGAAAAAGAA TTAAATGAAA AGCTTAAAGA ATATGTAATT ATTGCTGCAA CTAGTCGTAA TAATACATTA AAAGCCGATA CAAACTCATT GAAAGCTTTG CTGTATACCT TAAACGATCC TGCTGCTTCA AGTAAAATAA AAGATAGTTT CATTGAGGTA CTTGGAATAG ATAAAAATAA AGATGTACGA TTTGATAAAA CTAAAACCTT TGATGAAAAT ATTGCTAAGT TTAAATTTAC TAAACAAGAT TTAGAAAGCT TTATAACTAA AAGTAAAAGC GAAGCACCAA AAATTCAAAG CAAATATAGT GTAGCCAGAA GTCATGTAAG TGGGGGTGAT

Details for RifeA.17851.a.B1.GE36163

HARVESTED ON:	9/26/2012
SEQUENCED ON:	9/25/2012
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	59

Validated AA Sequence

Validated NT Sequence

tttntttnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatggtgca gtataaggat ttagtaatta cagcaactca gcaagagaca agcgaactaa ctattttcaa tagctttgat acgccaaata ttgaaattgc tttagcttgc cgtgcttctg cttctatacc gcttgttttt gaaccggtag aaattgacgg taaaaaatat gtcgacggcg gatatagaga taatataccg acaaaatatt ttaaaggcaa tgaaccggaa tttgatacaa aagaagtaac tgataatatg gaaaagatta ccttagctaa aaagcaaggt agaactctag ctatggcttt tggcacgggt atggaagctg atgcaaatat tgctatatat agtgcaaaaa aatttgaaag cccaagtgat atcgtaaagt ttttagctga tgtattattt aagactcttg caaaagtagg aggaaaattt aaatataccg aaactctaag agaaactaat gagcagttac gtgaaaatgc tttaaacact gttgttttag atactgccgg aatagatact ttggatttca aagatgcaca aaaatattct gattatttga atattaaagg atattgtcaa actagagaac atttgataat catgnccttg gcaaagaagc agataaaaca tttggaacta aattattaga aatgtttatt tcgtcaaaag aaaataatgc ttccaaatgg caagacggaa ttatcgaaaa tcatgacgaa agcaaaaatg ctattatcat tttgtaaaac cggagcatta aacgaaaaag aattaaatga aaagcttaaa gaatatgtaa ttattgctgc nactagtcgt aataatacat taaaagccga tacaactcat tgaaagcttt gctgtatacc ttaacgatcc tgctgcntca ntaaataaan atagtttcnt tgnnnantgn nagannaatn angnngtacg atttganaaa nnanannttg ntgaaatncn nntttnannt tactnannnn attnnannnt ttnnannaaa ntnaannnnc nnnnaannnn nnnnnnnnnn ancnnnannn nngnnnnann nnnnnnnnnn nnnnnnnnnn nnnnnnnnan nnna

Expected Protein Sequence

MAHHHHHHMV QYKDLVITAT QQETSELTIF NSFDTPNIEI ALACRASASI PLVFEPVEID GKKYVDGGYR DNIPTKYFKG NEPEFDTKEV TDNMEKITLA KKQGRTLAMA FGTGMEADAN IAIYSAKKFE SPSDIVKFLA DVLFKTLAKV GGKFKYTETL RETNEQLREN ALNTVVLDTA GIDTLDFKDA QKYSDYLNIK GYCQTREHLN NHDLGKEADK TFGTKLLEMF ISSKENNASK WQDGIIENHD DKAKMLLSFC KTGALNEKEL NEKLKEYVII AATSRNNTLK ADTNSLKALL YTLNDPAASS KIKDSFIEVL GIDKNKDVRF DKTKTFDENI AKFKFTKQDL ESFITKSKSE APKIQSKYSV ARSHVSGGD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc agtataagga tttagtaatt acagcaactc agcaagagac aagcgaacta actattttca atagctttga tacgccaaat attgaaattg ctttagcttg ccgtgcttct gcttctatac cgcttgtttt tgaaccggta gaaattgacg gtaaaaaata tgtcgacggc ggatatagag ataatatacc gacaaaatat tttaaaggca atgaaccgga atttgataca aaagaagtaa ctgataatat ggaaaagatt accttagcta aaaagcaagg tagaactcta gctatggctt ttggcacggg tatggaagct gatgcaaata ttgctatata tagtgcaaaa aaatttgaaa gcccaagtga tatcgtaaag tttttagctg atgtattatt taagactctt gcaaaagtag gaggaaaatt taaatatacc gaaactctaa gagaaactaa tgagcagtta cgtgaaaatg ctttaaacac tgttgtttta gatactgccg gaatagatac tttggatttc aaagatgcac aaaaatattc tgattatttg aatattaaag gatattgtca aactagagaa catttgaata atcatgacct tggcaaagaa gcagataaaa catttggaac taaattatta gaaatgttta tttcgtcaaa agaaaataat gcttccaaat ggcaagacgg aattatcgaa aatcatgacg ataaagcaaa aatgctatta tcattttgta aaaccggagc attaaacgaa aaagaattaa atgaaaagct taaagaatat gtaattattg ctgcaactag tcgtaataat acattaaaag ccgatacaaa ctcattgaaa gctttgctgt ataccttaaa cgatcctgct gcttcaagta aaataaaaga tagtttcatt gaggtacttg gaatagataa aaataaagat gtacgatttg ataaaactaa aacctttgat gaaaatattg ctaagtttaa atttactaaa caagatttag aaagctttat aactaaaagt aaaagcgaag caccaaaaat tcaaagcaaa tatagtgtag ccagaagtca tgtaagtggg ggtgattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t