RifeA.17834.b

Putative uncharacterized protein

CENTER ID: RifeA.17834.b
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.17834.b.B1.GE36162 Full length( RifeA.17834.b ) 1 534
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.17834.b.B1.PS01688 Full length( RifeA.17834.b ) 1 534

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.41
RefSeq: YP_246050.1
UniProt: Q4UNG9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKQANKISY DEVPYSPFTF SYTSPPYLRT IGKLFGLNPS PLETAKVLDI GCGVGVNLLN FAETYPKSQS LGVDLSKTQI ELGKKIISDL KIKNAELKAL SILDLDESYG KFDYIVCHGV YSWVPKEVQD KILEVCNKLL NPNGIAFVSY NTLPGWNMQR TIREMMMFHS ELFNTSHDKL QQARLLLKFI NDSLESSTTP YSNFLRDETK LLSAYTDSYV LHEYLGEINT GTYFHQFIEK AQNNNLNYLG DTSITAMFIG NLPTKAAEQL QAVNDIVRTE QYMDFITNRK FRSTLLCHQN IPINRKIEFN NLKGFYTSLN IRPVTPEKDV DLTNEQENIS FYYENLPDPF ISTTSPIIKA ILYVYAENIS NPISLEQVAK EAFKKLGKYQ LQDFLATLEQ HFITFIFQGY LKIFETKPHA IATITEKPKT SEFARYQTKQ AHFNNVTSVF SVTNRLNDMV GIPIHEKYIL EMLDGTHNID DIKKGVLEKI NSKLLTARDD KGQEVTDPKL LKEFVDYVVT ASLEKFRMSY MLVG
NT Sequence
ATGACAAAAC AAGCTAATAA AATATCCTAC GATGAAGTAC CTTACTCTCC ATTTACTTTC AGTTATACCT CCCCACCGTA TTTAAGAACT ATCGGTAAGC TATTTGGACT AAACCCTTCG CCTCTTGAAA CTGCTAAAGT ACTTGATATC GGTTGTGGAG TTGGTGTTAA TCTGCTTAAC TTTGCTGAAA CATATCCTAA ATCGCAATCT CTAGGTGTTG ACTTATCCAA AACCCAAATA GAATTAGGTA AAAAAATTAT CAGTGATTTG AAAATAAAAA ACGCCGAACT AAAAGCATTA TCTATATTGG ACCTTGATGA GTCATACGGT AAGTTTGATT ATATAGTTTG TCACGGCGTA TATTCGTGGG TGCCTAAAGA AGTACAAGAT AAAATACTTG AAGTCTGTAA TAAACTGCTA AATCCAAACG GTATCGCTTT CGTAAGCTAT AATACGCTGC CTGGCTGGAA TATGCAGAGA ACAATACGCG AAATGATGAT GTTCCATTCA GAGCTATTTA ATACTAGCCA CGATAAATTA CAGCAAGCTA GATTACTTTT AAAGTTTATC AATGACTCAT TAGAAAGTTC TACAACTCCT TATTCTAATT TCCTACGAGA TGAAACAAAA TTATTATCCG CATATACGGA CTCGTATGTA TTACATGAAT ATTTAGGCGA AATTAATACA GGTACATATT TTCACCAATT CATAGAAAAA GCTCAAAACA ATAATCTAAA TTACTTAGGC GATACGTCGA TTACTGCAAT GTTTATCGGT AATTTACCGA CTAAAGCAGC AGAACAACTA CAAGCTGTTA ACGATATTGT TCGTACAGAG CAATATATGG ATTTTATCAC TAATAGAAAA TTCCGTTCGA CATTATTATG TCATCAAAAC ATACCTATAA ACAGAAAAAT TGAATTTAAT AATTTAAAAG GGTTTTATAC TAGCCTTAAT ATTAGACCAG TAACTCCGGA AAAAGACGTT GATTTAACTA ACGAGCAGGA AAATATTAGC TTCTATTACG AAAATTTACC TGATCCGTTT ATTTCAACTA CTTCACCGAT TATAAAAGCT ATTTTATATG TATATGCTGA AAATATTAGC AATCCGATAA GCTTAGAACA AGTAGCAAAA GAAGCTTTTA AAAAGCTTGG TAAATATCAA TTACAAGATT TCTTAGCAAC ATTAGAACAG CATTTTATCA CATTCATTTT TCAAGGTTAT CTAAAAATAT TTGAGACCAA GCCGCATGCT ATAGCAACTA TCACCGAAAA ACCCAAAACT AGCGAATTTG CAAGATACCA AACAAAACAA GCACATTTTA ATAATGTAAC TAGCGTGTTT AGCGTCACTA ATAGACTTAA CGATATGGTA GGTATACCGA TACATGAAAA ATATATTTTA GAAATGTTAG ATGGTACGCA TAATATTGAT GATATTAAGA AAGGCGTTCT TGAAAAAATT AATTCAAAAC TTTTAACTGC TCGTGATGAT AAAGGGCAAG AAGTAACTGA TCCAAAACTA TTAAAAGAAT TTGTTGACTA TGTAGTTACT GCATCCCTTG AGAAATTTCG GATGAGCTAT ATGTTAGTAG GG
Details for RifeA.17834.b.B1.GE36162
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 62kDa
OBSERVED MW: 62kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 67
Validated AA Sequence
MQRTIREMMM FHSELFNTSH DKLQQARLLL KFINDSXESS TTPYSNFLRD ETKLLSAYTD SYVLHEYLXE INTGTYFHQF IEKAQNNNLN XXGDTSITAM FIGNLPTKAA EQLQAVNDIV RTEQYMDFIT NRKFRSTLLC HQNIPINRKI EFNNLKGFYT SLNIRPVTPE KDVDLTNEQE NISFYYENLP DPFISTTSPI IKAILYVYAE NISNPISLEQ VAKEAFKKLG KYQLQDFLAT LEQHFITFIF QGYLKIFETK PHAIATITEK PKTSEFARYQ TKQAHFNNVT SVFSVTNRLN DMVGIPIHEK YILEMLDGTH NIDDIKKGVL EKINSKLLTA RDDKGQEVTD PKLLKEFVDY VVTASL
Validated NT Sequence
ctcaagggat gcagtaacta catagtcaac aaattctttt aatagttttg gatcagttac ttcttgccct ttatcatcac gagcagttaa aagttttgaa ttaatttttt caagaacgcc tttcttaata tcatcaatat tatgcgtacc atctaacatt tctaaaatat atttttcatg tatcggtata cctaccatat cgttaagtct attagtgacg ctaaacacgc tagttacatt attaaaatgt gcttgttttg tttggtatct tgcaaattcg ctagttttgg gtttttcggt gatagttgct atagcatgcg gcttggtctc aaatattttt agataacctt gaaaaatgaa tgtgataaaa tgctgttcta atgttgctaa gaaatcttgt aattgatatt taccaagctt tttaaaagct tcttttgcta cttgttctaa gcttatcgga ttgctaatat tttcagcata tacatataaa atagctttta taatcggtga agtagttgaa ataaacggat caggtaaatt ttcgtaatag aagctaatat tttcctgctc gttagttaaa tcaacgtctt tttccggagt tactggtcta atattaaggc tagtataaaa cccttttaaa ttattaaatt caatttttct gtttataggt atgttttgat gacataataa tgtcgaacgg aattttctat tagtgataaa atccatatat tgctctgtac gaacaatatc gttaacagct tgtagttgtt ctgctgcttt agtcggtaaa ttaccgataa acattgcagt aatcgacgta tcgccnaant aatttagatt attgttttga gctttttcta tgaattggtg aaaatatgta cctgtattaa tttcgcntaa atattcatgt aatacatacg agtcngtata tgcggataat aattttgttt catctcgtag gaaattagaa taaggagttg tagaactttc tantgagtca ttgataaact ttaaaagtaa tctagcttgc tgtaatttat cgtggctagt attaaatagc tctgaatgga acatcatcat ttcgcgtatt gttctctgca tattccagcc aggcagcgta ttatagctta cgaaagcgat accgtttgga tttagcagtt tattacagac ttcaagtatt ttatcttgta cttctttagg cacccacgaa tatacgccgt gacaaactat ataatcaaac ttaccgtatg actcatcaag gtccaatata gataatgctt ttagttcggc gttttttatt ttcaaatcac tgataatttt tttacctaat tctatttggg ttttggataa gtcaacacct agagattgcg atttaggata tgtttcagca aagttaagca gattaacacc aactccacaa ccgatatcaa gtactttagc agtttcaaga ggcgaagggt ttagtccaaa tagcttaccg atagttctta aatacggtgg ggaggtataa ctgaaagtaa atggagagta aggtacttca tcgtaggata ttttattagc ttg
Expected Protein Sequence
MAHHHHHHMT KQANKISYDE VPYSPFTFSY TSPPYLRTIG KLFGLNPSPL ETAKVLDIGC GVGVNLLNFA ETYPKSQSLG VDLSKTQIEL GKKIISDLKI KNAELKALSI LDLDESYGKF DYIVCHGVYS WVPKEVQDKI LEVCNKLLNP NGIAFVSYNT LPGWNMQRTI REMMMFHSEL FNTSHDKLQQ ARLLLKFIND SLESSTTPYS NFLRDETKLL SAYTDSYVLH EYLGEINTGT YFHQFIEKAQ NNNLNYLGDT SITAMFIGNL PTKAAEQLQA VNDIVRTEQY MDFITNRKFR STLLCHQNIP INRKIEFNNL KGFYTSLNIR PVTPEKDVDL TNEQENISFY YENLPDPFIS TTSPIIKAIL YVYAENISNP ISLEQVAKEA FKKLGKYQLQ DFLATLEQHF ITFIFQGYLK IFETKPHAIA TITEKPKTSE FARYQTKQAH FNNVTSVFSV TNRLNDMVGI PIHEKYILEM LDGTHNIDDI KKGVLEKINS KLLTARDDKG QEVTDPKLLK EFVDYVVTAS LEKFRMSYML VG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa aacaagctaa taaaatatcc tacgatgaag taccttactc tccatttact ttcagttata cctccccacc gtatttaaga actatcggta agctatttgg actaaaccct tcgcctcttg aaactgctaa agtacttgat atcggttgtg gagttggtgt taatctgctt aactttgctg aaacatatcc taaatcgcaa tctctaggtg ttgacttatc caaaacccaa atagaattag gtaaaaaaat tatcagtgat ttgaaaataa aaaacgccga actaaaagca ttatctatat tggaccttga tgagtcatac ggtaagtttg attatatagt ttgtcacggc gtatattcgt gggtgcctaa agaagtacaa gataaaatac ttgaagtctg taataaactg ctaaatccaa acggtatcgc tttcgtaagc tataatacgc tgcctggctg gaatatgcag agaacaatac gcgaaatgat gatgttccat tcagagctat ttaatactag ccacgataaa ttacagcaag ctagattact tttaaagttt atcaatgact cattagaaag ttctacaact ccttattcta atttcctacg agatgaaaca aaattattat ccgcatatac ggactcgtat gtattacatg aatatttagg cgaaattaat acaggtacat attttcacca attcatagaa aaagctcaaa acaataatct aaattactta ggcgatacgt cgattactgc aatgtttatc ggtaatttac cgactaaagc agcagaacaa ctacaagctg ttaacgatat tgttcgtaca gagcaatata tggattttat cactaataga aaattccgtt cgacattatt atgtcatcaa aacataccta taaacagaaa aattgaattt aataatttaa aagggtttta tactagcctt aatattagac cagtaactcc ggaaaaagac gttgatttaa ctaacgagca ggaaaatatt agcttctatt acgaaaattt acctgatccg tttatttcaa ctacttcacc gattataaaa gctattttat atgtatatgc tgaaaatatt agcaatccga taagcttaga acaagtagca aaagaagctt ttaaaaagct tggtaaatat caattacaag atttcttagc aacattagaa cagcatttta tcacattcat ttttcaaggt tatctaaaaa tatttgagac caagccgcat gctatagcaa ctatcaccga aaaacccaaa actagcgaat ttgcaagata ccaaacaaaa caagcacatt ttaataatgt aactagcgtg tttagcgtca ctaatagact taacgatatg gtaggtatac cgatacatga aaaatatatt ttagaaatgt tagatggtac gcataatatt gatgatatta agaaaggcgt tcttgaaaaa attaattcaa aacttttaac tgctcgtgat gataaagggc aagaagtaac tgatccaaaa ctattaaaag aatttgttga ctatgtagtt actgcatccc ttgagaaatt tcggatgagc tatatgttag tagggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for RifeA.17834.b.B1.PS01688
PURIFICATION DATe: 11/29/2012
CONCENTRATION: 40.4mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 67
Protocol Notes
notes unavailable
Validated AA Sequence
MQRTIREMMM FHSELFNTSH DKLQQARLLL KFINDSXESS TTPYSNFLRD ETKLLSAYTD SYVLHEYLXE INTGTYFHQF IEKAQNNNLN XXGDTSITAM FIGNLPTKAA EQLQAVNDIV RTEQYMDFIT NRKFRSTLLC HQNIPINRKI EFNNLKGFYT SLNIRPVTPE KDVDLTNEQE NISFYYENLP DPFISTTSPI IKAILYVYAE NISNPISLEQ VAKEAFKKLG KYQLQDFLAT LEQHFITFIF QGYLKIFETK PHAIATITEK PKTSEFARYQ TKQAHFNNVT SVFSVTNRLN DMVGIPIHEK YILEMLDGTH NIDDIKKGVL EKINSKLLTA RDDKGQEVTD PKLLKEFVDY VVTASL
Validated NT Sequence
ctcaagggat gcagtaacta catagtcaac aaattctttt aatagttttg gatcagttac ttcttgccct ttatcatcac gagcagttaa aagttttgaa ttaatttttt caagaacgcc tttcttaata tcatcaatat tatgcgtacc atctaacatt tctaaaatat atttttcatg tatcggtata cctaccatat cgttaagtct attagtgacg ctaaacacgc tagttacatt attaaaatgt gcttgttttg tttggtatct tgcaaattcg ctagttttgg gtttttcggt gatagttgct atagcatgcg gcttggtctc aaatattttt agataacctt gaaaaatgaa tgtgataaaa tgctgttcta atgttgctaa gaaatcttgt aattgatatt taccaagctt tttaaaagct tcttttgcta cttgttctaa gcttatcgga ttgctaatat tttcagcata tacatataaa atagctttta taatcggtga agtagttgaa ataaacggat caggtaaatt ttcgtaatag aagctaatat tttcctgctc gttagttaaa tcaacgtctt tttccggagt tactggtcta atattaaggc tagtataaaa cccttttaaa ttattaaatt caatttttct gtttataggt atgttttgat gacataataa tgtcgaacgg aattttctat tagtgataaa atccatatat tgctctgtac gaacaatatc gttaacagct tgtagttgtt ctgctgcttt agtcggtaaa ttaccgataa acattgcagt aatcgacgta tcgccnaant aatttagatt attgttttga gctttttcta tgaattggtg aaaatatgta cctgtattaa tttcgcntaa atattcatgt aatacatacg agtcngtata tgcggataat aattttgttt catctcgtag gaaattagaa taaggagttg tagaactttc tantgagtca ttgataaact ttaaaagtaa tctagcttgc tgtaatttat cgtggctagt attaaatagc tctgaatgga acatcatcat ttcgcgtatt gttctctgca tattccagcc aggcagcgta ttatagctta cgaaagcgat accgtttgga tttagcagtt tattacagac ttcaagtatt ttatcttgta cttctttagg cacccacgaa tatacgccgt gacaaactat ataatcaaac ttaccgtatg actcatcaag gtccaatata gataatgctt ttagttcggc gttttttatt ttcaaatcac tgataatttt tttacctaat tctatttggg ttttggataa gtcaacacct agagattgcg atttaggata tgtttcagca aagttaagca gattaacacc aactccacaa ccgatatcaa gtactttagc agtttcaaga ggcgaagggt ttagtccaaa tagcttaccg atagttctta aatacggtgg ggaggtataa ctgaaagtaa atggagagta aggtacttca tcgtaggata ttttattagc ttg
Expressed Protein Sequence
MAHHHHHHMT KQANKISYDE VPYSPFTFSY TSPPYLRTIG KLFGLNPSPL ETAKVLDIGC GVGVNLLNFA ETYPKSQSLG VDLSKTQIEL GKKIISDLKI KNAELKALSI LDLDESYGKF DYIVCHGVYS WVPKEVQDKI LEVCNKLLNP NGIAFVSYNT LPGWNMQRTI REMMMFHSEL FNTSHDKLQQ ARLLLKFIND SLESSTTPYS NFLRDETKLL SAYTDSYVLH EYLGEINTGT YFHQFIEKAQ NNNLNYLGDT SITAMFIGNL PTKAAEQLQA VNDIVRTEQY MDFITNRKFR STLLCHQNIP INRKIEFNNL KGFYTSLNIR PVTPEKDVDL TNEQENISFY YENLPDPFIS TTSPIIKAIL YVYAENISNP ISLEQVAKEA FKKLGKYQLQ DFLATLEQHF ITFIFQGYLK IFETKPHAIA TITEKPKTSE FARYQTKQAH FNNVTSVFSV TNRLNDMVGI PIHEKYILEM LDGTHNIDDI KKGVLEKINS KLLTARDDKG QEVTDPKLLK EFVDYVVTAS LEKFRMSYML VG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAA AACAAGCTAA TAAAATATCC TACGATGAAG TACCTTACTC TCCATTTACT TTCAGTTATA CCTCCCCACC GTATTTAAGA ACTATCGGTA AGCTATTTGG ACTAAACCCT TCGCCTCTTG AAACTGCTAA AGTACTTGAT ATCGGTTGTG GAGTTGGTGT TAATCTGCTT AACTTTGCTG AAACATATCC TAAATCGCAA TCTCTAGGTG TTGACTTATC CAAAACCCAA ATAGAATTAG GTAAAAAAAT TATCAGTGAT TTGAAAATAA AAAACGCCGA ACTAAAAGCA TTATCTATAT TGGACCTTGA TGAGTCATAC GGTAAGTTTG ATTATATAGT TTGTCACGGC GTATATTCGT GGGTGCCTAA AGAAGTACAA GATAAAATAC TTGAAGTCTG TAATAAACTG CTAAATCCAA ACGGTATCGC TTTCGTAAGC TATAATACGC TGCCTGGCTG GAATATGCAG AGAACAATAC GCGAAATGAT GATGTTCCAT TCAGAGCTAT TTAATACTAG CCACGATAAA TTACAGCAAG CTAGATTACT TTTAAAGTTT ATCAATGACT CATTAGAAAG TTCTACAACT CCTTATTCTA ATTTCCTACG AGATGAAACA AAATTATTAT CCGCATATAC GGACTCGTAT GTATTACATG AATATTTAGG CGAAATTAAT ACAGGTACAT ATTTTCACCA ATTCATAGAA AAAGCTCAAA ACAATAATCT AAATTACTTA GGCGATACGT CGATTACTGC AATGTTTATC GGTAATTTAC CGACTAAAGC AGCAGAACAA CTACAAGCTG TTAACGATAT TGTTCGTACA GAGCAATATA TGGATTTTAT CACTAATAGA AAATTCCGTT CGACATTATT ATGTCATCAA AACATACCTA TAAACAGAAA AATTGAATTT AATAATTTAA AAGGGTTTTA TACTAGCCTT AATATTAGAC CAGTAACTCC GGAAAAAGAC GTTGATTTAA CTAACGAGCA GGAAAATATT AGCTTCTATT ACGAAAATTT ACCTGATCCG TTTATTTCAA CTACTTCACC GATTATAAAA GCTATTTTAT ATGTATATGC TGAAAATATT AGCAATCCGA TAAGCTTAGA ACAAGTAGCA AAAGAAGCTT TTAAAAAGCT TGGTAAATAT CAATTACAAG ATTTCTTAGC AACATTAGAA CAGCATTTTA TCACATTCAT TTTTCAAGGT TATCTAAAAA TATTTGAGAC CAAGCCGCAT GCTATAGCAA CTATCACCGA AAAACCCAAA ACTAGCGAAT TTGCAAGATA CCAAACAAAA CAAGCACATT TTAATAATGT AACTAGCGTG TTTAGCGTCA CTAATAGACT TAACGATATG GTAGGTATAC CGATACATGA AAAATATATT TTAGAAATGT TAGATGGTAC GCATAATATT GATGATATTA AGAAAGGCGT TCTTGAAAAA ATTAATTCAA AACTTTTAAC TGCTCGTGAT GATAAAGGGC AAGAAGTAAC TGATCCAAAA CTATTAAAAG AATTTGTTGA CTATGTAGTT ACTGCATCCC TTGAGAAATT TCGGATGAGC TATATGTTAG TAGGGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT