SSGCID: Target Page

RifeA.17834.a

Putative uncharacterized protein

CENTER ID:	RifeA.17834.a
ORGANISM:	Rickettsia felis URRWXCal2
ASSOCIATED DISEASE:	Flea-borne spotted fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.17834.a.B1.GE36161	Full length( RifeA.17834.a )		1	509

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|315456.7.peg.1506
RefSeq:	YP_247270.1
UniProt:	Q4UK30

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSKATNSSS TPNGHDKTVK TTKPVLNGAV SNHNTYDEVP YESYPYALTN PYHLSTLATL FGVNAPEVEN AKILELGCAA GGNLIPHAVL YPKAHFVGVD LSKVQVDEAN KNVKELGLKN IEFHHCSITD IDDSFGKFDY IICHGVISWV PKTVRDKIFE VCNKNLSPNG IAYISYNTLP GWNMVRTIRD MMMYHSSSFA NVRDRIAQSR LLLEFVKDSL ENSKTPYAEV LKTEAGLLAK QTDHYLRHDH LEEENAQFYF HEFMNEARKH NLQYLADCNL STMYLGNMPL KVVEQLKAVN DIVRTEQYMD FITNRRFRTT LLCHSDMKIN RNINNDDITK FNMIFNIVPE KPIKEVDLNN ASEALKFFLN GNKDSNLTTS SPYMKAILYT FSENLNNPLS FEKITSEANK KLHSTKLNEI KAELLNNAMK LVLQGYISIT NQKHRNNPEL DKPKTTKMVI HQATHTPSMW VTNLKHEPIG VNFFEKFALR YMDGKHDKKA IIEGPYLVM

NT Sequence

ATGTCCTCAA AAGCTACTAA CTCTTCTTCT ACCCCTAACG GTCATGATAA GACAGTAAAA ACTACCAAGC CTGTTCTGAA TGGTGCGGTT TCAAATCATA ATACTTATGA TGAAGTGCCG TATGAAAGCT ACCCATATGC TCTTACAAAT CCTTATCACT TAAGTACACT TGCAACTCTT TTCGGTGTGA ATGCTCCTGA AGTCGAGAAT GCAAAAATAT TAGAGCTTGG TTGTGCAGCG GGCGGTAATT TAATACCACA TGCAGTTCTT TATCCAAAAG CTCATTTTGT TGGGGTTGAT TTGTCTAAGG TACAAGTTGA CGAAGCAAAT AAAAATGTTA AAGAACTAGG ATTAAAAAAT ATAGAGTTCC ATCATTGTTC GATAACCGAT ATTGATGATT CTTTCGGTAA GTTTGATTAT ATAATTTGCC ATGGCGTAAT TTCTTGGGTA CCAAAAACCG TTAGAGATAA AATTTTTGAA GTTTGTAATA AAAATCTTAG TCCAAACGGA ATAGCATATA TTAGCTATAA TACCCTCCCT GGCTGGAATA TGGTCCGTAC TATTAGAGAT ATGATGATGT ATCATTCTAG CTCATTTGCA AATGTACGTG ATAGAATAGC TCAATCTAGA TTGTTACTAG AATTTGTTAA GGATAGCTTA GAAAATTCTA AAACTCCTTA TGCAGAAGTA TTAAAAACCG AAGCAGGGCT GCTTGCTAAA CAAACCGATC ATTATTTACG TCATGATCAT CTAGAAGAAG AAAATGCTCA ATTCTACTTC CATGAATTTA TGAATGAAGC AAGAAAGCAC AATTTACAAT ATTTAGCCGA TTGTAATCTT TCGACTATGT ATCTTGGTAA TATGCCGCTA AAAGTAGTAG AGCAGTTAAA AGCGGTGAAC GATATAGTTA GAACTGAACA ATATATGGAT TTCATTACGA ATCGTCGTTT TAGAACCACT TTACTATGTC ATAGTGATAT GAAAATTAAT AGAAATATTA ATAATGATGA TATAACGAAA TTTAATATGA TCTTTAATAT AGTGCCTGAA AAGCCGATTA AAGAAGTAGA TCTTAATAAT GCTTCCGAAG CCTTAAAATT CTTCTTAAAC GGTAATAAAG ATTCTAATTT AACAACTAGT TCGCCTTACA TGAAAGCTAT TTTATACACT TTCAGCGAGA ATCTAAATAA TCCGTTAAGC TTTGAGAAAA TAACCTCTGA AGCCAATAAA AAGCTACATA GTACTAAATT AAACGAAATT AAAGCTGAGC TTTTAAATAA TGCAATGAAG CTAGTACTAC AAGGTTATAT TAGTATTACA AATCAGAAGC ATCGAAATAA CCCTGAGCTT GATAAGCCTA AAACAACAAA AATGGTGATA CATCAAGCAA CTCATACACC TTCTATGTGG GTTACAAATT TAAAACATGA ACCTATCGGC GTTAATTTCT TTGAGAAGTT TGCACTTAGA TATATGGATG GTAAACATGA TAAAAAAGCA ATTATTGAGG GGCCGTACTT GGTCATG

Details for RifeA.17834.a.B1.GE36161

HARVESTED ON:	9/26/2012
SEQUENCED ON:	9/25/2012
EXPECTED MW:	59kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	54

Validated AA Sequence

Validated NT Sequence

attttntnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatgtcc tcaaaagcta ctaactcttc ttctacccct aacggtcatg ataagacagt aaaaactacc aagcctgttc tgaatggtgc ggtttcaaat cataatactt atgatgaagt gccgtatgaa agctacccat atgctcttac aaatccttat cacttaagta cacttgcaac tcttttcggt gtgaatgctc ctgaagtcga gaatgcaaaa atattagagc ttggttgtgc agcgggcggt aatttaatac cacatgcagt tctttatcca aaagctcatt ttgttggggt tgatttgtct aaggtacaag ttgacgaagc aaataaaaat gttaaagaac taggattaaa aaatatagag ttccatcatt gttcgataac cgatattgat gattctttcg gtaagtttga ttatataatt tgccatggcg taatttcttg ggtaccaaaa accgttagag ataaaatttt tgaagtttgt aataaaaatc ttagtccaaa cggaatagca tatattagct ataataccct ccctggctgg aatatggtcc gtactattag agatatgatg atgtatcatt ctagctcatt tgcaaatgta cgtgatagaa tagctcaatc tagattgtta ctagaatttg ttaaggatag cttagaaaat tctaaaactc cttatgcaga agtattaaaa accgaagcag ggctgcttgc taaacaaacc gatcattatt tacgtcatga tcatctagaa gaagaaaatg ctcaattcta cttccatgaa tttatgaatg aagcnngaaa gcacaattta canatttagc cnantgnaat ctttcgacta tgtatcntgg tatatgncnc naaantagta gagcagttaa agcgnngacg anatagttag antganannn atggatttca ttacgnantc gtcntttnna ccncttacnn tgtncnnnng ntnngnaant nannnnnnnn gatgannnna cnnnntnann nnnnnnntnn nnnnnnnnng nnaannnnnn nnannaannn nnnnnantna ngnnnnnnnn cnnnnanntn anngnnnnnn nannannnnn ntncnntttt nnnnnnnntt nnnnnnnnnn annnnccnnt nnnnntnnna nnnnnnnnnn nnnnnnnnna

Expected Protein Sequence

MAHHHHHHMS SKATNSSSTP NGHDKTVKTT KPVLNGAVSN HNTYDEVPYE SYPYALTNPY HLSTLATLFG VNAPEVENAK ILELGCAAGG NLIPHAVLYP KAHFVGVDLS KVQVDEANKN VKELGLKNIE FHHCSITDID DSFGKFDYII CHGVISWVPK TVRDKIFEVC NKNLSPNGIA YISYNTLPGW NMVRTIRDMM MYHSSSFANV RDRIAQSRLL LEFVKDSLEN SKTPYAEVLK TEAGLLAKQT DHYLRHDHLE EENAQFYFHE FMNEARKHNL QYLADCNLST MYLGNMPLKV VEQLKAVNDI VRTEQYMDFI TNRRFRTTLL CHSDMKINRN INNDDITKFN MIFNIVPEKP IKEVDLNNAS EALKFFLNGN KDSNLTTSSP YMKAILYTFS ENLNNPLSFE KITSEANKKL HSTKLNEIKA ELLNNAMKLV LQGYISITNQ KHRNNPELDK PKTTKMVIHQ ATHTPSMWVT NLKHEPIGVN FFEKFALRYM DGKHDKKAII EGPYLVM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct caaaagctac taactcttct tctaccccta acggtcatga taagacagta aaaactacca agcctgttct gaatggtgcg gtttcaaatc ataatactta tgatgaagtg ccgtatgaaa gctacccata tgctcttaca aatccttatc acttaagtac acttgcaact cttttcggtg tgaatgctcc tgaagtcgag aatgcaaaaa tattagagct tggttgtgca gcgggcggta atttaatacc acatgcagtt ctttatccaa aagctcattt tgttggggtt gatttgtcta aggtacaagt tgacgaagca aataaaaatg ttaaagaact aggattaaaa aatatagagt tccatcattg ttcgataacc gatattgatg attctttcgg taagtttgat tatataattt gccatggcgt aatttcttgg gtaccaaaaa ccgttagaga taaaattttt gaagtttgta ataaaaatct tagtccaaac ggaatagcat atattagcta taataccctc cctggctgga atatggtccg tactattaga gatatgatga tgtatcattc tagctcattt gcaaatgtac gtgatagaat agctcaatct agattgttac tagaatttgt taaggatagc ttagaaaatt ctaaaactcc ttatgcagaa gtattaaaaa ccgaagcagg gctgcttgct aaacaaaccg atcattattt acgtcatgat catctagaag aagaaaatgc tcaattctac ttccatgaat ttatgaatga agcaagaaag cacaatttac aatatttagc cgattgtaat ctttcgacta tgtatcttgg taatatgccg ctaaaagtag tagagcagtt aaaagcggtg aacgatatag ttagaactga acaatatatg gatttcatta cgaatcgtcg ttttagaacc actttactat gtcatagtga tatgaaaatt aatagaaata ttaataatga tgatataacg aaatttaata tgatctttaa tatagtgcct gaaaagccga ttaaagaagt agatcttaat aatgcttccg aagccttaaa attcttctta aacggtaata aagattctaa tttaacaact agttcgcctt acatgaaagc tattttatac actttcagcg agaatctaaa taatccgtta agctttgaga aaataacctc tgaagccaat aaaaagctac atagtactaa attaaacgaa attaaagctg agcttttaaa taatgcaatg aagctagtac tacaaggtta tattagtatt acaaatcaga agcatcgaaa taaccctgag cttgataagc ctaaaacaac aaaaatggtg atacatcaag caactcatac accttctatg tgggttacaa atttaaaaca tgaacctatc ggcgttaatt tctttgagaa gtttgcactt agatatatgg atggtaaaca tgataaaaaa gcaattattg aggggccgta cttggtcatg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat