RifeA.17832.b

Cell surface antigen-like protein Sca11

CENTER ID: RifeA.17832.b
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.17832.b.B1.GE36159 Full length( RifeA.17832.b ) 1 333
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.370
RefSeq: YP_246335.1
UniProt: Q4UMN8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQTAAKKDEE IVNTMYNPTI VHNMNQLNSN IISNRIMHTT LPASAVAAGD EDEKVLDKGI WLASIYGTNK QGVQKEFIGY KGRTSGGTIG FDVGFENVND LLGIAYTKLD SKFKSKGKNL NTKIDSHIVA LYGQKELPKN FMMQAMFAYN HNIINSKINR LGTVANGKYK NNNYNFEGLL SYNYLLNSSI SLTPNIGIKY GYSKDGQYQE GNTGIQSLSV AAKKHNLWSS VLGGKVILTP QKIYKNINIT PALQASVENY FHNTSKKLHT KVKWKDKEIN ETVALPKQPK IGYNIGASVL TEKDKVSVLL EYNCHLQKKY QSHQGFIKLK INL
NT Sequence
ATGCAAACAG CAGCAAAAAA AGATGAAGAG ATAGTGAATA CAATGTATAA TCCAACTATT GTTCATAATA TGAATCAGCT GAACAGTAAT ATTATATCAA ACAGAATTAT GCATACAACT TTACCTGCGT CTGCAGTTGC TGCCGGTGAT GAAGATGAAA AAGTTTTAGA TAAAGGCATC TGGCTTGCTA GTATTTACGG TACTAATAAA CAAGGAGTGC AAAAAGAATT TATTGGCTAT AAAGGGCGTA CTTCCGGTGG TACAATTGGT TTTGATGTTG GATTTGAGAA TGTAAATGAT CTGCTAGGAA TTGCTTACAC TAAACTCGAT TCAAAGTTTA AATCTAAAGG TAAAAACTTA AATACTAAAA TTGATAGCCA TATAGTAGCT TTGTACGGCC AGAAAGAATT ACCGAAAAAC TTTATGATGC AAGCGATGTT TGCTTATAAC CATAATATAA TAAATAGTAA AATTAACCGT TTGGGTACTG TTGCCAACGG TAAATATAAA AATAATAATT ACAATTTTGA AGGCTTGTTA AGTTATAATT ATCTCTTGAA TAGTAGTATT TCACTTACTC CAAATATTGG TATAAAGTAC GGTTATTCTA AAGATGGTCA GTATCAAGAA GGTAATACCG GTATACAGAG TTTAAGCGTA GCAGCCAAAA AACATAATTT ATGGAGCAGT GTTTTAGGTG GAAAAGTAAT TTTAACTCCG CAAAAAATAT ATAAGAACAT AAATATTACT CCGGCATTAC AAGCATCTGT AGAAAATTAC TTTCACAATA CAAGTAAAAA ACTTCATACT AAGGTAAAAT GGAAAGATAA AGAAATCAAT GAAACAGTTG CTTTACCAAA ACAACCTAAA ATAGGTTATA ATATTGGTGC CAGCGTCTTG ACGGAAAAAG ATAAGGTAAG TGTTCTACTA GAATATAACT GCCACTTACA GAAGAAATAT CAAAGCCATC AAGGTTTTAT AAAGTTAAAA ATTAATTTG
Details for RifeA.17832.b.B1.GE36159
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMQ TAAKKDEEIV NTMYNPTIVH NMNQLNSNII SNRIMHTTLP ASAVAAGDED EKVLDKGIWL ASIYGTNKQG VQKEFIGYKG RTSGGTIGFD VGFENVNDLL GIAYTKLDSK FKSKGKNLNT KIDSHIVALY GQKELPKNFM MQAMFAYNHN IINSKINRLG TVANGKYKNN NYNFEGLLSY NYLLNSSISL TPNIGIKYGY SKDGQYQEXN TGIQSLSVAA KKHNLWSSVL XGKVILTPQK IYKNINITPA LQASVENYFH NTSKKLHTXX KWKDKEINET VALPKQPKXX IIXVPASXXK X
Validated NT Sequence
cnnnnnnnnn nntttntnnn nnttaagaag gaganatacc atggctcacc accaccacca ccatatgcaa acagcagcaa aaaaagatga agagatagtg aatacaatgt ataatccaac tattgttcat aatatgaatc agctgaacag taatattata tcaaacagaa ttatgcatac aactttacct gcgtctgcag ttgctgccgg tgatgaagat gaaaaagttt tagataaagg catctggctt gctagtattt acggtactaa taaacaagga gtgcaaaaag aatttattgg ctataaaggg cgtacttccg gtggtacaat tggttttgat gttggatttg agaatgtaaa tgatctgcta ggaattgctt acactaaact cgattcaaag tttaaatcta aaggtaaaaa cttaaatact aaaattgata gccatatagt agctttgtac ggccagaaag aattaccgaa aaactttatg atgcaagcga tgtttgctta taaccataat ataataaata gtaaaattaa ccgtttgggt actgttgcca acggtaaata taaaaataat aattacaatt ttgaaggctt gttaagttat aattatctct tgaatagtag tatttcactt actccaaata ttggtataaa gtacggttat tctaaagatg gtcagtatca agaangtaat accggtatac agagtttaag cgtagcagcc aaaaaacata atttatggag cagtgtttta ngtggaaaag taattttaac tccgcaaaaa atatataaga acataaatat tactccngca ttacaagcat ctgtagaaaa ttactttcac aatacaagta aaaaacttca tactaannta aaatggaaag ataaagaaat caatgaaaca gttgctttac caaaacaacc taaaatnnnt ataatantgg tgccagcgtc tnganggaaa anataannaa gtgnnctact agannntnac tgccncttac nnaanaaann tcnaagccnt caaggttttn nnantnaant nnnnnantna ntnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnanaaa aaaaaaaaaa aaaaaa
Expected Protein Sequence
MAHHHHHHMQ TAAKKDEEIV NTMYNPTIVH NMNQLNSNII SNRIMHTTLP ASAVAAGDED EKVLDKGIWL ASIYGTNKQG VQKEFIGYKG RTSGGTIGFD VGFENVNDLL GIAYTKLDSK FKSKGKNLNT KIDSHIVALY GQKELPKNFM MQAMFAYNHN IINSKINRLG TVANGKYKNN NYNFEGLLSY NYLLNSSISL TPNIGIKYGY SKDGQYQEGN TGIQSLSVAA KKHNLWSSVL GGKVILTPQK IYKNINITPA LQASVENYFH NTSKKLHTKV KWKDKEINET VALPKQPKIG YNIGASVLTE KDKVSVLLEY NCHLQKKYQS HQGFIKLKIN L
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa cagcagcaaa aaaagatgaa gagatagtga atacaatgta taatccaact attgttcata atatgaatca gctgaacagt aatattatat caaacagaat tatgcataca actttacctg cgtctgcagt tgctgccggt gatgaagatg aaaaagtttt agataaaggc atctggcttg ctagtattta cggtactaat aaacaaggag tgcaaaaaga atttattggc tataaagggc gtacttccgg tggtacaatt ggttttgatg ttggatttga gaatgtaaat gatctgctag gaattgctta cactaaactc gattcaaagt ttaaatctaa aggtaaaaac ttaaatacta aaattgatag ccatatagta gctttgtacg gccagaaaga attaccgaaa aactttatga tgcaagcgat gtttgcttat aaccataata taataaatag taaaattaac cgtttgggta ctgttgccaa cggtaaatat aaaaataata attacaattt tgaaggcttg ttaagttata attatctctt gaatagtagt atttcactta ctccaaatat tggtataaag tacggttatt ctaaagatgg tcagtatcaa gaaggtaata ccggtataca gagtttaagc gtagcagcca aaaaacataa tttatggagc agtgttttag gtggaaaagt aattttaact ccgcaaaaaa tatataagaa cataaatatt actccggcat tacaagcatc tgtagaaaat tactttcaca atacaagtaa aaaacttcat actaaggtaa aatggaaaga taaagaaatc aatgaaacag ttgctttacc aaaacaacct aaaataggtt ataatattgg tgccagcgtc ttgacggaaa aagataaggt aagtgttcta ctagaatata actgccactt acagaagaaa tatcaaagcc atcaaggttt tataaagtta aaaattaatt tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat