RifeA.17522.a
ADP-ribose pyrophosphatase MutT
| CENTER ID: | RifeA.17522.a |
| ORGANISM: | Rickettsia felis URRWXCal2 |
| ASSOCIATED DISEASE: | Flea-borne spotted fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RifeA.17522.a.B1.GE35892 | Full length( RifeA.17522.a ) | 1 | 141 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4KYX
DEPOSITED: 5/29/2013
DETERMINATION: XRay
CLONE: RifeA.17522.a.B1.GE35892
PROTEIN: RifeA.17522.a.B1.PW36475
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|315456.7.peg.710 |
| RefSeq: | YP_246611.1 |
| UniProt: | Q4ULX7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MINHPRIGIG ILIFNNRNEI LLGKRISSHG ESSYAPAGGH LEFGETFEEC AIREVLEETN LIIENPQFIA VTNDIFEKEQ KHYVSIFLKA HCLNEHELQN LEPHKVENWQ WFALDNLPSN LFLPLKRLIE KKCYLYKEII D
NT Sequence
ATGATTAACC ACCCACGGAT CGGTATCGGT ATTTTAATCT TTAATAATAG GAATGAGATT TTATTAGGTA AACGTATTAG TTCCCATGGT GAATCTAGTT ATGCTCCTGC CGGTGGACAT TTAGAATTTG GGGAAACATT TGAAGAATGT GCTATCCGTG AAGTTTTAGA AGAAACAAAT CTAATCATTG AAAACCCACA ATTTATAGCG GTGACTAACG ATATTTTTGA AAAAGAACAA AAGCATTACG TATCAATTTT CCTTAAAGCT CATTGTTTAA ATGAGCATGA ATTACAGAAT CTTGAGCCTC ATAAGGTAGA GAACTGGCAG TGGTTTGCTC TTGATAATTT ACCTTCTAAC CTCTTCCTAC CCTTAAAAAG ACTTATTGAG AAAAAATGTT ATCTATACAA AGAAATTATT GAT
Details for RifeA.17522.a.B1.GE35892
| HARVESTED ON: | 8/14/2012 |
| SEQUENCED ON: | 8/18/2012 |
| EXPECTED MW: | 17kDa |
| OBSERVED MW: | 16kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMI NHPRIGIGIL IFNNRNEILL GKRISSHGES SYAPAGGHLE FGETFEECAI REVLEETNLI IENPQFIAVT NDIFEKEQKH YVSIFLKAHC LNEHELQNLE PHKVENWQWF ALDNLPSNLF LPLKRLIEKK CYLYKEIID
Validated NT Sequence
atcttactca cttaatcaat aatttctttg tatagataac attttttctc aataagtctt tttaagggta ggaagaggtt agaaggtaaa ttatcaagag caaaccactg ccagttctct accttatgag gctcaagatt ctgtaattca tgctcattta aacaatgagc tttaaggaaa attgatacgt aatgcttttg ttctttttca aaaatatcgt tagtcaccgc tataaattgt gggttttcaa tgattagatt tgtttcttct aaaacttcac ggatagcaca ttcttcaaat gtttccccaa attctaaatg tccaccggca ggagcataac tagattcacc atgggaacta atacgtttac ctaataaaat ctcattccta ttattaaaga ttaaaatacc gataccgatc cgtgggtggt taatcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMI NHPRIGIGIL IFNNRNEILL GKRISSHGES SYAPAGGHLE FGETFEECAI REVLEETNLI IENPQFIAVT NDIFEKEQKH YVSIFLKAHC LNEHELQNLE PHKVENWQWF ALDNLPSNLF LPLKRLIEKK CYLYKEIID
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatta accacccacg gatcggtatc ggtattttaa tctttaataa taggaatgag attttattag gtaaacgtat tagttcccat ggtgaatcta gttatgctcc tgccggtgga catttagaat ttggggaaac atttgaagaa tgtgctatcc gtgaagtttt agaagaaaca aatctaatca ttgaaaaccc acaatttata gcggtgacta acgatatttt tgaaaaagaa caaaagcatt acgtatcaat tttccttaaa gctcattgtt taaatgagca tgaattacag aatcttgagc ctcataaggt agagaactgg cagtggtttg ctcttgataa tttaccttct aacctcttcc tacccttaaa aagacttatt gagaaaaaat gttatctata caaagaaatt attgattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t