RifeA.17347.a

Poly-beta-hydroxyalkanoate depolymerase

CENTER ID: RifeA.17347.a
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.17347.a.B1.GE35890 Full length( RifeA.17347.a ) 1 425
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.273
RefSeq: YP_246254.1
UniProt: Q4UMW9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMNCDLSTNY TYYILEGLRA QIAPIHLGIK ILKEAYEHES LEDNGFARIM HSYLTLCERM TRKYEKPEFN ILETIIDDKT YNINEKVILK KSFCELRHFQ KIGFKKELPK LLIVAPMAGH HATLLRSTVQ ELLPYTDIYI TDWTEASFVP LEAGHFDMDD YIDYVMEFIN FIGPNVHTMA VCQPTVPLLA AISLMSESNS PNVPSSMILM GGPIDARKNP TAVNEFAQSK SLEWFCKMVT MQVPPNYPGH GRKVYPGFLQ LAGFMSLNLF RHIDSHLELW QSLLNSDYKK ADHTIKFYDE YLAGMDMPAE FYLQTIDEVF QQFSLARGKL VSEKRPIDLK HITKCALLGI EGELDDIAAV GQTKAALKLC SNIPESMKRY HLQKGAGHYG VFSGSKFKQF IVPIIKDFIY EFDKTNFKQS KLKAV
NT Sequence
ATGATGAACT GTGATTTAAG TACTAATTAT ACATATTATA TATTAGAGGG GCTTAGAGCA CAAATTGCTC CAATACATTT AGGTATAAAA ATCCTGAAAG AGGCGTATGA ACATGAGTCT CTAGAGGATA ATGGGTTTGC AAGAATTATG CATTCTTATT TAACGCTTTG TGAACGCATG ACTAGAAAAT ATGAAAAACC CGAATTTAAT ATTCTTGAAA CAATTATTGA TGATAAAACC TATAACATTA ATGAAAAAGT TATACTAAAA AAGTCTTTTT GTGAACTAAG GCATTTTCAG AAAATAGGTT TTAAAAAAGA ATTACCTAAA TTATTAATAG TAGCACCCAT GGCGGGGCAT CACGCTACTT TACTTAGATC AACCGTACAA GAATTATTAC CGTATACCGA TATTTATATT ACAGATTGGA CGGAAGCAAG TTTCGTGCCG CTTGAAGCAG GGCATTTTGA TATGGATGAT TATATTGATT ATGTAATGGA GTTTATCAAT TTTATAGGAC CTAATGTTCA CACTATGGCA GTTTGCCAAC CTACTGTTCC ACTGCTTGCC GCCATTAGCT TAATGTCGGA GAGTAATAGC CCGAATGTGC CGAGTTCGAT GATTTTGATG GGTGGACCTA TTGATGCTAG AAAAAATCCC ACTGCAGTTA ATGAATTTGC TCAAAGTAAA AGTTTAGAAT GGTTCTGCAA AATGGTTACA ATGCAGGTGC CGCCAAACTA TCCAGGTCAT GGAAGAAAAG TATATCCCGG CTTTCTCCAA CTTGCCGGCT TTATGAGTTT AAACTTATTC CGTCATATAG ATTCACATTT AGAATTATGG CAAAGTTTAT TAAATTCTGA TTATAAAAAA GCCGATCATA CTATTAAATT TTATGATGAG TATTTAGCAG GTATGGATAT GCCGGCAGAG TTTTACTTAC AAACTATAGA TGAAGTATTC CAACAATTTT CATTAGCAAG AGGTAAATTA GTTTCTGAGA AGCGTCCGAT TGATTTAAAA CATATTACTA AATGTGCTTT ACTTGGTATT GAAGGGGAAT TAGATGATAT TGCAGCGGTA GGGCAAACAA AAGCTGCTTT AAAACTTTGT TCAAATATAC CTGAATCAAT GAAAAGATAC CATTTACAAA AAGGGGCAGG GCATTACGGG GTATTTAGCG GAAGTAAATT TAAACAGTTT ATAGTACCTA TTATCAAAGA TTTTATCTAT GAATTTGATA AAACTAATTT CAAGCAATCT AAACTTAAGG CAGTA
Details for RifeA.17347.a.B1.GE35890
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 49kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMM NCDLSTNYTY YILEGLRAQI APIHLGIKIL KEAYEHESLE DNGFARIMHS YLTLCERMTR KYEKPEFNIL ETIIDDKTYN INEKVILKKS FCELRHFQKI GFKKELPKLL IVAPIAGHHA TLLRSTVQEL LPYTDIYITD WTEASFVPLE AGHFDMDDYI DYVMEFINFI GPNVHTMAVC QPTVPLLAAI SLMSESNSPN VPSSMILMGG PIDARKNPTA VNEFAQSKSL EWFCKMVTMX VPPNYPGHGR KVYPGFLQLA GFMSLNLFRH IDSHLELWQS LLNSDXKKAD HTIKFYDEYL AGMXXPAXFY LXXIDXXSXX SXXSNXX
Validated NT Sequence
attttgtnnn nnttaagaag gaganatacc atggctcacc accaccacca ccatatgatg aactgtgatt taagtactaa ttatacatat tatatattag aggggcttag agcacaaatt gctccaatac atttaggtat aaaaatcctg aaagaggcgt atgaacatga gtctctagag gataatgggt ttgcaagaat tatgcattct tatttaacgc tttgtgaacg catgactaga aaatatgaaa aacccgaatt taatattctt gaaacaatta ttgatgataa aacctataac attaatgaaa aagttatact aaaaaagtct ttttgtgaac taaggcattt tcagaaaata ggttttaaaa aagaattacc taaattatta atagtagcac ccatagcggg gcatcacgct actttactta gatcaaccgt acaagaatta ttaccgtata ccgatattta tattacagat tggacggaag caagtttcgt gccgcttgaa gcagggcatt ttgatatgga tgattatatt gattatgtaa tggagtttat caattttata ggacctaatg ttcacactat ggcagtttgc caacctactg ttccactgct tgccgccatt agcttaatgt cggagagtaa tagcccgaat gtgccgagtt cgatgatttt gatgggtgga cctattgatg ctagaaaaaa tcccactgca gttaatgaat ttgctcaaag taaaagttta gaatggttct gcaaaatggt tacaatgcan gtgccgccaa actatccagg tcatggaaga aaagtatatc ccggctttct ccaacttgcc ggctttatga gtttaaactt attccgtcat atagattcac atttagaatt atggcaaagt ttattaaatt ctgattanaa aaaagccgat catactatta aattttatga tgagtattta gcnggnatgg ntntgccggc aganttttac ttannancta tagatganna ntcnnnnntt tcntnncana gtaatanttn ntganancgn cnatgattaa anntntnnna angnnnntnn ntntnnnnnn nnnatnannn nncanngnnn gnnnnnanct gnnnnnnnnn tnnnnnnnnn nnnnannnan nnnnnnnnnn nnnnnnngnn nnnttnnngn nntt
Expected Protein Sequence
MAHHHHHHMM NCDLSTNYTY YILEGLRAQI APIHLGIKIL KEAYEHESLE DNGFARIMHS YLTLCERMTR KYEKPEFNIL ETIIDDKTYN INEKVILKKS FCELRHFQKI GFKKELPKLL IVAPMAGHHA TLLRSTVQEL LPYTDIYITD WTEASFVPLE AGHFDMDDYI DYVMEFINFI GPNVHTMAVC QPTVPLLAAI SLMSESNSPN VPSSMILMGG PIDARKNPTA VNEFAQSKSL EWFCKMVTMQ VPPNYPGHGR KVYPGFLQLA GFMSLNLFRH IDSHLELWQS LLNSDYKKAD HTIKFYDEYL AGMDMPAEFY LQTIDEVFQQ FSLARGKLVS EKRPIDLKHI TKCALLGIEG ELDDIAAVGQ TKAALKLCSN IPESMKRYHL QKGAGHYGVF SGSKFKQFIV PIIKDFIYEF DKTNFKQSKL KAV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatga actgtgattt aagtactaat tatacatatt atatattaga ggggcttaga gcacaaattg ctccaataca tttaggtata aaaatcctga aagaggcgta tgaacatgag tctctagagg ataatgggtt tgcaagaatt atgcattctt atttaacgct ttgtgaacgc atgactagaa aatatgaaaa acccgaattt aatattcttg aaacaattat tgatgataaa acctataaca ttaatgaaaa agttatacta aaaaagtctt tttgtgaact aaggcatttt cagaaaatag gttttaaaaa agaattacct aaattattaa tagtagcacc catggcgggg catcacgcta ctttacttag atcaaccgta caagaattat taccgtatac cgatatttat attacagatt ggacggaagc aagtttcgtg ccgcttgaag cagggcattt tgatatggat gattatattg attatgtaat ggagtttatc aattttatag gacctaatgt tcacactatg gcagtttgcc aacctactgt tccactgctt gccgccatta gcttaatgtc ggagagtaat agcccgaatg tgccgagttc gatgattttg atgggtggac ctattgatgc tagaaaaaat cccactgcag ttaatgaatt tgctcaaagt aaaagtttag aatggttctg caaaatggtt acaatgcagg tgccgccaaa ctatccaggt catggaagaa aagtatatcc cggctttctc caacttgccg gctttatgag tttaaactta ttccgtcata tagattcaca tttagaatta tggcaaagtt tattaaattc tgattataaa aaagccgatc atactattaa attttatgat gagtatttag caggtatgga tatgccggca gagttttact tacaaactat agatgaagta ttccaacaat tttcattagc aagaggtaaa ttagtttctg agaagcgtcc gattgattta aaacatatta ctaaatgtgc tttacttggt attgaagggg aattagatga tattgcagcg gtagggcaaa caaaagctgc tttaaaactt tgttcaaata tacctgaatc aatgaaaaga taccatttac aaaaaggggc agggcattac ggggtattta gcggaagtaa atttaaacag tttatagtac ctattatcaa agattttatc tatgaatttg ataaaactaa tttcaagcaa tctaaactta aggcagtatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat