RifeA.17300.a

Type I secretion outer membrane protein, TolC family

CENTER ID: RifeA.17300.a
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.17300.a.B2.GE35555 Mature protein( RifeA.17300.a ) 19 454
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.1177
RefSeq: YP_247001.1
UniProt: Q4UKT7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRKLTTFIIT LLLTGSAAAV DLQEALTEGY KNNEELKAAR IKFLNAIEQF PQAFSGFMPS AGMKIERSNS KTKYNKKYTD RLGLTPRETD SNQGALTIEQ SLFSGGSSVA ALKAAQSGFR ASRGEYYAGE QKILLNLITA YLDCFESKEK YDISESRVRT NIQQVNTVEE KLRLGEATAI DIATARAGLA AAETNKLAAY ADFQGKKANF IRVFGIEATD ITMPNLPDRL PASLDELTKK AAKLNPDIDS ARHNVTSAKA LEMVQKGKLL PQVSVKLQSG RTNYNPQDPA VQNINNKSYT TILSVNIPIY PNGGAQYSEI RKAKNQTRNS AVQLDSAIKQ TQAGVVSVWE GFEAAKSRIV AANQGVEAAQ ISYDGTVQEE IVGSKTILDV LDAEQKLYEA KITRVDAYKN SVLAAYQMKL LTGELTAQSL KLKVKYFSPE EEFKTIKKKM FIGF
NT Sequence
ATGCGTAAAT TAACTACATT TATTATCACC TTATTACTAA CCGGTTCAGC TGCTGCTGTT GATTTGCAGG AAGCTTTAAC TGAAGGATAT AAGAATAATG AGGAGCTGAA AGCTGCTAGA ATTAAGTTTT TGAATGCGAT TGAGCAGTTC CCTCAAGCTT TTTCAGGATT TATGCCTAGT GCCGGAATGA AGATTGAAAG AAGTAATAGT AAAACCAAAT ATAATAAAAA ATATACTGAT AGGCTTGGTC TTACTCCAAG AGAGACAGAT AGCAATCAAG GTGCATTAAC AATTGAGCAA TCATTATTTA GCGGTGGTTC TAGTGTTGCG GCTCTTAAAG CTGCTCAATC AGGGTTTAGA GCATCACGAG GCGAATATTA TGCCGGCGAA CAAAAAATAT TATTAAATTT AATAACTGCT TATCTTGATT GTTTTGAGAG CAAAGAGAAA TATGATATTT CTGAAAGTAG GGTTCGTACT AACATACAAC AAGTTAACAC TGTTGAAGAG AAATTAAGAC TAGGGGAAGC AACGGCGATA GATATAGCAA CTGCAAGAGC AGGGCTTGCA GCGGCAGAAA CGAATAAACT AGCTGCCTAT GCCGATTTTC AAGGAAAAAA AGCAAATTTT ATTAGAGTAT TCGGAATAGA GGCTACTGAT ATAACTATGC CTAATTTACC TGATAGATTA CCTGCTTCAT TAGATGAGTT AACAAAAAAA GCTGCTAAGT TAAATCCTGA TATTGATTCG GCAAGGCATA ATGTAACTTC GGCGAAGGCT TTGGAAATGG TGCAGAAAGG AAAATTACTG CCGCAAGTGA GCGTAAAGTT GCAATCAGGT AGAACTAACT ATAATCCACA AGATCCAGCT GTTCAAAATA TAAACAATAA AAGCTATACT ACTATTCTTT CGGTAAATAT CCCTATTTAT CCAAATGGAG GGGCTCAATA TTCAGAAATT AGAAAAGCTA AAAACCAAAC AAGAAATAGT GCAGTTCAGC TTGATAGTGC GATAAAGCAG ACACAAGCGG GTGTTGTAAG TGTATGGGAA GGATTTGAAG CGGCAAAATC TCGTATTGTT GCAGCTAATC AAGGCGTGGA GGCTGCACAA ATATCATATG ACGGTACAGT GCAAGAGGAA ATAGTCGGCT CCAAAACTAT ACTCGATGTT TTAGATGCTG AGCAAAAATT GTATGAAGCA AAAATAACTC GTGTGGATGC GTATAAGAAT TCAGTACTTG CGGCATATCA AATGAAATTG TTAACCGGTG AGCTAACGGC TCAAAGTTTA AAACTTAAAG TAAAATATTT TAGTCCTGAA GAAGAGTTTA AGACTATTAA AAAGAAAATG TTTATAGGTT TC
Details for RifeA.17300.a.B2.GE35555
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 87
Validated AA Sequence
MPSAGMKIER SNSKTKYNKK YTDRLGLTPR ETDSNQGALT IEQSLFSGGS SVAALKAAQS GFRASRGEYY AGEQKILLNL ITAYLDCFES KEKYDISESR VRTNIQQVNT VEEKLRLGEA TAIDIATARA GLAAAETNKL AAYADFQGKK ANFIRVFGIE ATDITMPNLP DRLPASLDEL TKKAAKLNPD IDSARHNVTS AKALEMVQKG KLLPQVSVKL QSGRTNYNPQ DPAVQNINNK SYTTILSVNI PIYPNGGAQY SEIRKAKNQT RNSAVQLDSA IKQTQAGVVS VWEGFEAAKS RIVAANQGVE AAQISYDGTV QEEIVGSKTI LDVLDAEQKL YEAKITRVDA YKNSVLAAYQ MKLLTGELTA QSLKLKVKYF SPEEEFKT
Validated NT Sequence
aatagtctta aactcttctt caggactaaa atattttact ttaagtttta aactttgagc cgttagctca ccggttaaca atttcatttg atatgccgca agtactgaat tcttatacgc atccacacga gttatttttg cttcatacaa tttttgctca gcatctaaaa catcgagtat agttttggag ccgactattt cctcttgcac tgtaccgtca tatgatattt gtgcagcctc cacgccttga ttagctgcaa caatacgaga ttttgccgct tcaaatcctt cccatacact tacaacaccc gcttgtgtct gctttatcgc actatcaagc tgaactgcac tatttcttgt ttggttttta gcttttctaa tttctgaata ttgagcccct ccatttggat aaatagggat atttaccgaa agaatagtag tatagctttt attgtttata ttttgaacag ctggatcttg tggattatag ttagttctac ctgattgcaa ctttacgctc acttgcggca gtaattttcc tttctgcacc atttccaaag ccttcgccga agttacatta tgccttgccg aatcaatatc aggatttaac ttagcagctt tttttgttaa ctcatctaat gaagcaggta atctatcagg taaattaggc atagttatat cagtagcctc tattccgaat actctaataa aatttgcttt ttttccttga aaatcggcat aggcagctag tttattcgtt tctgccgctg caagccctgc tcttgcagtt gctatatcta tcgccgttgc ttcccctagt cttaatttct cttcaacagt gttaacttgt tgtatgttag tacgaaccct actttcagaa atatcatatt tctctttgct ctcaaaacaa tcaagataag cagttattaa atttaataat attttttgtt cgccggcata atattcgcct cgtgatgctc taaaccctga ttgagcagct ttaagagccg caacactaga accaccgcta aataatgatt gctcaattgt taatgcacct tgattgctat ctgtctctct tggagtaaga ccaagcctat cagtatattt tttattatat ttggttttac tattacttct ttcaatcttc attccggcac taggcataaa tcctgaaaaa gcttgaggga actgctcaat cgcattcaaa aacttaattc tagcagcttt cagctcctca ttattcttat atccttcagt taaagcttcc tgcaaatcaa cagcc
Expected Protein Sequence
MAHHHHHHMA VDLQEALTEG YKNNEELKAA RIKFLNAIEQ FPQAFSGFMP SAGMKIERSN SKTKYNKKYT DRLGLTPRET DSNQGALTIE QSLFSGGSSV AALKAAQSGF RASRGEYYAG EQKILLNLIT AYLDCFESKE KYDISESRVR TNIQQVNTVE EKLRLGEATA IDIATARAGL AAAETNKLAA YADFQGKKAN FIRVFGIEAT DITMPNLPDR LPASLDELTK KAAKLNPDID SARHNVTSAK ALEMVQKGKL LPQVSVKLQS GRTNYNPQDP AVQNINNKSY TTILSVNIPI YPNGGAQYSE IRKAKNQTRN SAVQLDSAIK QTQAGVVSVW EGFEAAKSRI VAANQGVEAA QISYDGTVQE EIVGSKTILD VLDAEQKLYE AKITRVDAYK NSVLAAYQMK LLTGELTAQS LKLKVKYFSP EEEFKTIKKK MFIGF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg ttgatttgca ggaagcttta actgaaggat ataagaataa tgaggagctg aaagctgcta gaattaagtt tttgaatgcg attgagcagt tccctcaagc tttttcagga tttatgccta gtgccggaat gaagattgaa agaagtaata gtaaaaccaa atataataaa aaatatactg ataggcttgg tcttactcca agagagacag atagcaatca aggtgcatta acaattgagc aatcattatt tagcggtggt tctagtgttg cggctcttaa agctgctcaa tcagggttta gagcatcacg aggcgaatat tatgccggcg aacaaaaaat attattaaat ttaataactg cttatcttga ttgttttgag agcaaagaga aatatgatat ttctgaaagt agggttcgta ctaacataca acaagttaac actgttgaag agaaattaag actaggggaa gcaacggcga tagatatagc aactgcaaga gcagggcttg cagcggcaga aacgaataaa ctagctgcct atgccgattt tcaaggaaaa aaagcaaatt ttattagagt attcggaata gaggctactg atataactat gcctaattta cctgatagat tacctgcttc attagatgag ttaacaaaaa aagctgctaa gttaaatcct gatattgatt cggcaaggca taatgtaact tcggcgaagg ctttggaaat ggtgcagaaa ggaaaattac tgccgcaagt gagcgtaaag ttgcaatcag gtagaactaa ctataatcca caagatccag ctgttcaaaa tataaacaat aaaagctata ctactattct ttcggtaaat atccctattt atccaaatgg aggggctcaa tattcagaaa ttagaaaagc taaaaaccaa acaagaaata gtgcagttca gcttgatagt gcgataaagc agacacaagc gggtgttgta agtgtatggg aaggatttga agcggcaaaa tctcgtattg ttgcagctaa tcaaggcgtg gaggctgcac aaatatcata tgacggtaca gtgcaagagg aaatagtcgg ctccaaaact atactcgatg ttttagatgc tgagcaaaaa ttgtatgaag caaaaataac tcgtgtggat gcgtataaga attcagtact tgcggcatat caaatgaaat tgttaaccgg tgagctaacg gctcaaagtt taaaacttaa agtaaaatat tttagtcctg aagaagagtt taagactatt aaaaagaaaa tgtttatagg tttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat