SSGCID: Target Page

RifeA.10014.f

Ribonucleotide ABC transporter ATP-binding protein

CENTER ID:	RifeA.10014.f
ORGANISM:	Rickettsia felis URRWXCal2
ASSOCIATED DISEASE:	Flea-borne spotted fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.10014.f.B1.GE35881	Full length( RifeA.10014.f )		1	252

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|315456.7.peg.93
RefSeq:	YP_246097.1
UniProt:	Q4UNC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTKEEFKIK IRSLYKSFAN HKVLDGIDLD VKKGSSLVIL GGSGSGKSVL IKNIVGLIKP DKGKIFIDNV EIQDISSKQK FEIMDGIGFL FQGGALFDSL NIRDNITFET RKLSKKEKND LAGAKLNSVG LSPRILDLYP SELSGGMQKR VALARAICST PSILFLDEPT TGLDPIMANV INELIIKIQE ELGATTITIT HDMISAEKIA KEVAMIYQGK IKWYGSKDEM RNSDNPYLKQ FINGLTTGPI EV

NT Sequence

ATGAGTACAA AGGAAGAATT TAAAATTAAA ATTCGGTCAT TATATAAATC ATTTGCTAAT CATAAGGTGT TAGACGGAAT AGATTTGGAT GTAAAAAAGG GCAGTTCATT AGTTATTTTA GGCGGTTCCG GTAGTGGCAA ATCGGTGCTA ATTAAAAATA TAGTAGGACT GATTAAACCT GATAAAGGTA AAATTTTTAT TGATAATGTA GAAATCCAAG ATATCTCAAG TAAACAAAAA TTTGAGATTA TGGACGGTAT AGGTTTTTTA TTTCAAGGCG GAGCATTATT TGACTCCTTG AATATACGTG ATAATATTAC TTTCGAGACT AGAAAATTAT CTAAGAAAGA AAAAAACGAC CTTGCCGGTG CAAAGCTTAA TTCCGTCGGT TTGTCCCCTA GAATACTTGA TCTTTACCCT TCCGAATTAT CGGGCGGAAT GCAAAAAAGA GTAGCTCTTG CTAGGGCTAT TTGTAGTACA CCGTCGATTT TATTTCTTGA TGAACCGACC ACAGGGCTTG ATCCTATAAT GGCAAATGTT ATCAACGAAT TAATTATAAA AATCCAAGAA GAGTTAGGGG CAACTACGAT TACTATAACT CATGATATGA TTAGTGCCGA AAAAATAGCT AAAGAAGTAG CTATGATTTA TCAAGGAAAA ATTAAATGGT ACGGTAGTAA AGATGAAATG CGTAATAGTG ATAATCCTTA TTTAAAACAA TTTATAAACG GATTAACTAC CGGTCCTATA GAGGTA

Details for RifeA.10014.f.B1.GE35881

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	28kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS TKEEFKIKIR SLYKSFANHK VLDGIDLDVK KGSSLVILGG SGSGKSVLIK NIVGLIKPDK GKIFIDNVEI QDISSKQKFE IMDGIGFLFQ GGALFDSLNI RDNITFETRK LSKKEKNDLA GAKLNSVGLS PRILDLYPSE LSGGMQKRVA LARAICSTPS ILFLDEPTTG LDPIMANVIN ELIIKIQEEL GATTITITHD MISAEKIAKE VAMIYQGKIK WYGSKDEMRN SDNPYLKQFI NGLTTGPIEV

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatgagt acaaaggaag aatttaaaat taaaattcgg tcattatata aatcatttgc taatcataag gtgttagacg gaatagattt ggatgtaaaa aagggcagtt cattagttat tttaggcggt tccggtagtg gcaaatcggt gctaattaaa aatatagtag gactgattaa acctgataaa ggtaaaattt ttattgataa tgtagaaatc caagatatct caagtaaaca aaaatttgag attatggacg gtataggttt tttatttcaa ggcggagcat tatttgactc cttgaatata cgtgataata ttactttcga gactagaaaa ttatctaaga aagaaaaaaa cgaccttgcc ggtgcaaagc ttaattccgt cggtttgtcc cctagaatac ttgatcttta cccttccgaa ttatcgggcg gaatgcaaaa aagagtagct cttgctaggg ctatttgtag tacaccgtcg attttatttc ttgatgaacc gaccacaggg cttgatccta taatggcaaa tgttatcaac gaattaatta taaaaatcca agaagagtta ggggcaacta cgattactat aactcatgat atgattagtg ccgaaaaaat agctaaagaa gtagctatga tttatcaagg aaaaattaaa tggtacggta gtaaagatga aatgcgtaat agtgataatc cttatttaaa acaatttata aacggattaa ctaccggtcc tatagaggta taagtgagta agataggatc cggnnnnnnn ncccccnnnn nnnnnannnn nnnnnnnaaa aaaaaaannn nngnnncnnn nnnnnnnnnn nnnnnnnnnn nnnnnancc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta caaaggaaga atttaaaatt aaaattcggt cattatataa atcatttgct aatcataagg tgttagacgg aatagatttg gatgtaaaaa agggcagttc attagttatt ttaggcggtt ccggtagtgg caaatcggtg ctaattaaaa atatagtagg actgattaaa cctgataaag gtaaaatttt tattgataat gtagaaatcc aagatatctc aagtaaacaa aaatttgaga ttatggacgg tataggtttt ttatttcaag gcggagcatt atttgactcc ttgaatatac gtgataatat tactttcgag actagaaaat tatctaagaa agaaaaaaac gaccttgccg gtgcaaagct taattccgtc ggtttgtccc ctagaatact tgatctttac ccttccgaat tatcgggcgg aatgcaaaaa agagtagctc ttgctagggc tatttgtagt acaccgtcga ttttatttct tgatgaaccg accacagggc ttgatcctat aatggcaaat gttatcaacg aattaattat aaaaatccaa gaagagttag gggcaactac gattactata actcatgata tgattagtgc cgaaaaaata gctaaagaag tagctatgat ttatcaagga aaaattaaat ggtacggtag taaagatgaa atgcgtaata gtgataatcc ttatttaaaa caatttataa acggattaac taccggtcct atagaggtat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat