SSGCID: Target Page

RifeA.01656.b

Outer membrane protein rOmpA

CENTER ID:	RifeA.01656.b
ORGANISM:	Rickettsia felis URRWXCal2
ASSOCIATED DISEASE:	Flea-borne spotted fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.01656.b.B1.GE35875	Full length( RifeA.01656.b )		1	189

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|315456.7.peg.1571
RefSeq:	YP_247318.1
UniProt:	Q4UJY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLEWMQYSPA AVAAGDEDRD AKFGAWISPF VGNATQKMRN NISGYKSDTT GGTIGFDGLV NDDLALGLAY TRADTDIKLK NNKTGDKNKV ESNIYSVYGL YNVPYENLFV EAIASYSDNR IKSKSRRVIA TALETVGYQT ASGKYKSESY TGQLMAGYTY MMPENINLTP LAGLRYSAIK DKGYKETDY

NT Sequence

ATGCTAGAAT GGATGCAATA CAGCCCGGCT GCCGTAGCTG CCGGTGATGA GGACAGAGAT GCTAAGTTTG GTGCATGGAT AAGTCCGTTT GTCGGTAATG CAACGCAGAA GATGCGTAAC AATATAAGCG GTTATAAGTC TGATACAACC GGTGGCACTA TAGGTTTTGA CGGCTTAGTT AACGATGATT TAGCACTCGG GCTTGCATAT ACAAGAGCCG ATACCGATAT TAAGCTGAAA AATAATAAGA CGGGCGATAA GAATAAGGTA GAGAGTAACA TCTATTCCGT ATACGGTTTA TATAATGTAC CTTATGAAAA TCTTTTCGTT GAAGCTATAG CATCTTACTC GGATAATAGG ATAAAAAGCA AATCAAGACG TGTTATTGCA ACGGCACTAG AGACTGTCGG CTATCAAACC GCAAGCGGTA AGTATAAATC TGAAAGCTAT ACAGGTCAGT TAATGGCTGG TTATACCTAT ATGATGCCTG AGAATATTAA CTTAACACCG CTTGCAGGGC TTAGATATTC GGCTATCAAA GATAAGGGCT ATAAGGAAAC TGACTAC

Details for RifeA.01656.b.B1.GE35875

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	21kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML EWMQYSPAAV AAGDEDRDAK FGAWISPFVG NATQKMRNNI SGYKSDTTGG TIGFDGLVND DLALGLAYTR ADTDIKLKNN KTGDKNKVES NIYSVYGLYN VPYENLFVEA IASYSDNRIK SKSRRVIATA LETVGYQTAS GKYKSESYTG QLMAGYTYMM PENINLTPLA GLRYSAIKDK GYKETDY

Validated NT Sequence

ttgtnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgctagaa tggatgcaat acagcccggc tgccgtagct gccggtgatg aggacagaga tgctaagttt ggtgcatgga taagtccgtt tgtcggtaat gcaacgcaga agatgcgtaa caatataagc ggttataagt ctgatacaac cggtggcact ataggttttg acggcttagt taacgatgat ttagcactcg ggcttgcata tacaagagcc gataccgata ttaagctgaa aaataataag acgggcgata agaataaggt agagagtaac atctattccg tatacggttt atataatgta ccttatgaaa atcttttcgt tgaagctata gcatcttact cggataatag gataaaaagc aaatcaagac gtgttattgc aacggcacta gagactgtcg gctatcaaac cgcaagcggt aagtataaat ctgaaagcta tacaggtcag ttaatggctg gttataccta tatgatgcct gagaatatta acttaacacc gcttgcaggg cttagatatt cggctatcaa agataagggc tataaggaaa ctgactacta agtgagtaag ataggatccg gctgctaaca nncccccnaa annnnnnann tgnggggnnn nnntncnnnn nnnnnnnntg ntnangagag tgtnaggngn nnanntgnnn nnnnnnnnnn nnggnnngnn nnnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctag aatggatgca atacagcccg gctgccgtag ctgccggtga tgaggacaga gatgctaagt ttggtgcatg gataagtccg tttgtcggta atgcaacgca gaagatgcgt aacaatataa gcggttataa gtctgataca accggtggca ctataggttt tgacggctta gttaacgatg atttagcact cgggcttgca tatacaagag ccgataccga tattaagctg aaaaataata agacgggcga taagaataag gtagagagta acatctattc cgtatacggt ttatataatg taccttatga aaatcttttc gttgaagcta tagcatctta ctcggataat aggataaaaa gcaaatcaag acgtgttatt gcaacggcac tagagactgt cggctatcaa accgcaagcg gtaagtataa atctgaaagc tatacaggtc agttaatggc tggttatacc tatatgatgc ctgagaatat taacttaaca ccgcttgcag ggcttagata ttcggctatc aaagataagg gctataagga aactgactac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat