RifeA.00940.a

Undecaprenyl pyrophosphate synthase

CENTER ID: RifeA.00940.a
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00940.a.B1.GE35868 Full length( RifeA.00940.a ) 1 235
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00940.a.B1.PW37280 Full length( RifeA.00940.a ) 1 235

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.786
RefSeq: YP_246672.1
UniProt: Q4ULR6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQQHLKYKIM TNIKHLAIIM DGNARWADQH NLTKSEGHKA GADKIRELLP EFINLNIPYI TLYTFSSENW QRSSTEVDFL IKLLSIYFKN ELNSLHKNGV KIKVIGRLNL LSSSLQKQIN NAIELTKNNN KITLCIAFSY GSRQEIVDAC TKIIASGKKE ISESDIGHAL YDPEMPDVDL LIRPGGVYRI SNFLLWQAAY AELYFSPKYW PAFNKDDIIE AINDYSKRKR TFGKR
NT Sequence
ATGCAGCAAC ATCTAAAATA TAAAATAATG ACTAACATAA AACATTTAGC AATAATAATG GACGGTAATG CTCGTTGGGC AGATCAGCAT AACTTAACAA AATCTGAAGG GCATAAAGCA GGAGCCGATA AAATTCGTGA ATTACTGCCT GAGTTTATTA ATCTTAATAT ACCTTATATA ACTTTATATA CTTTCTCTTC CGAAAATTGG CAACGTTCAA GCACGGAGGT AGATTTCTTA ATAAAATTAT TAAGTATTTA TTTTAAAAAT GAATTAAACA GTTTACATAA AAACGGCGTT AAAATAAAAG TAATAGGTAG GCTTAATTTA TTAAGTAGCT CATTGCAAAA ACAAATAAAT AATGCTATAG AATTAACAAA AAATAATAAT AAAATAACGC TTTGTATAGC TTTTAGTTAT GGAAGTCGTC AAGAAATAGT TGATGCCTGC ACAAAAATTA TTGCAAGCGG TAAAAAAGAA ATAAGTGAAA GCGATATAGG ACACGCTTTA TATGATCCTG AAATGCCGGA TGTAGATTTA TTAATAAGAC CGGGCGGGGT CTATCGCATC AGTAATTTCT TGCTTTGGCA AGCAGCTTAT GCTGAATTAT ATTTCTCACC CAAATATTGG CCCGCTTTTA ATAAAGATGA TATAATAGAA GCTATAAATG ATTACTCAAA AAGGAAAAGA ACATTTGGCA AAAGA
Details for RifeA.00940.a.B1.GE35868
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ QHLKYKIMTN IKHLAIIMDG NARWADQHNL TKSEGHKAGA DKIRELLPEF INLNIPYITL YTFSSENWQR SSTEVDFLIK LLSIYFKNEL NSLHKNGVKI KVIGRLNLLS SSLQKQINNA IELTKNNNKI TLCIAFSYGS RQEIVDACTK IIASGKKEIS ESDIGHALYD PEMPDVDLLI RPGGVYRISN FLLWQAAYAE LYFSPKYWPA FNKDDIIEAI NDYSKRKRTF GKR
Validated NT Sequence
atcttactca cttatctttt gccaaatgtt cttttccttt ttgagtaatc atttatagct tctattatat catctttatt aaaagcgggc caatatttgg gtgagaaata taattcagca taagctgctt gccaaagcaa gaaattactg atgcgataga ccccgcccgg tcttattaat aaatctacat ccggcatttc aggatcatat aaagcgtgtc ctatatcgct ttcacttatt tcttttttac cgcttgcaat aatttttgtg caggcatcaa ctatttcttg acgacttcca taactaaaag ctatacaaag cgttatttta ttattatttt ttgttaattc tatagcatta tttatttgtt tttgcaatga gctacttaat aaattaagcc tacctattac ttttatttta acgccgtttt tatgtaaact gtttaattca tttttaaaat aaatacttaa taattttatt aagaaatcta cctccgtgct tgaacgttgc caattttcgg aagagaaagt atataaagtt atataaggta tattaagatt aataaactca ggcagtaatt cacgaatttt atcggctcct gctttatgcc cttcagattt tgttaagtta tgctgatctg cccaacgagc attaccgtcc attattattg ctaaatgttt tatgttagtc attattttat attttagatg ttgctgcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMQ QHLKYKIMTN IKHLAIIMDG NARWADQHNL TKSEGHKAGA DKIRELLPEF INLNIPYITL YTFSSENWQR SSTEVDFLIK LLSIYFKNEL NSLHKNGVKI KVIGRLNLLS SSLQKQINNA IELTKNNNKI TLCIAFSYGS RQEIVDACTK IIASGKKEIS ESDIGHALYD PEMPDVDLLI RPGGVYRISN FLLWQAAYAE LYFSPKYWPA FNKDDIIEAI NDYSKRKRTF GKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagc aacatctaaa atataaaata atgactaaca taaaacattt agcaataata atggacggta atgctcgttg ggcagatcag cataacttaa caaaatctga agggcataaa gcaggagccg ataaaattcg tgaattactg cctgagttta ttaatcttaa tataccttat ataactttat atactttctc ttccgaaaat tggcaacgtt caagcacgga ggtagatttc ttaataaaat tattaagtat ttattttaaa aatgaattaa acagtttaca taaaaacggc gttaaaataa aagtaatagg taggcttaat ttattaagta gctcattgca aaaacaaata aataatgcta tagaattaac aaaaaataat aataaaataa cgctttgtat agcttttagt tatggaagtc gtcaagaaat agttgatgcc tgcacaaaaa ttattgcaag cggtaaaaaa gaaataagtg aaagcgatat aggacacgct ttatatgatc ctgaaatgcc ggatgtagat ttattaataa gaccgggcgg ggtctatcgc atcagtaatt tcttgctttg gcaagcagct tatgctgaat tatatttctc acccaaatat tggcccgctt ttaataaaga tgatataata gaagctataa atgattactc aaaaaggaaa agaacatttg gcaaaagatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for RifeA.00940.a.B1.PW37280
PURIFICATION DATe: 3/6/2013
CONCENTRATION: 25mg/ml
OBSERVED MW: 29kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ QHLKYKIMTN IKHLAIIMDG NARWADQHNL TKSEGHKAGA DKIRELLPEF INLNIPYITL YTFSSENWQR SSTEVDFLIK LLSIYFKNEL NSLHKNGVKI KVIGRLNLLS SSLQKQINNA IELTKNNNKI TLCIAFSYGS RQEIVDACTK IIASGKKEIS ESDIGHALYD PEMPDVDLLI RPGGVYRISN FLLWQAAYAE LYFSPKYWPA FNKDDIIEAI NDYSKRKRTF GKR
Validated NT Sequence
atcttactca cttatctttt gccaaatgtt cttttccttt ttgagtaatc atttatagct tctattatat catctttatt aaaagcgggc caatatttgg gtgagaaata taattcagca taagctgctt gccaaagcaa gaaattactg atgcgataga ccccgcccgg tcttattaat aaatctacat ccggcatttc aggatcatat aaagcgtgtc ctatatcgct ttcacttatt tcttttttac cgcttgcaat aatttttgtg caggcatcaa ctatttcttg acgacttcca taactaaaag ctatacaaag cgttatttta ttattatttt ttgttaattc tatagcatta tttatttgtt tttgcaatga gctacttaat aaattaagcc tacctattac ttttatttta acgccgtttt tatgtaaact gtttaattca tttttaaaat aaatacttaa taattttatt aagaaatcta cctccgtgct tgaacgttgc caattttcgg aagagaaagt atataaagtt atataaggta tattaagatt aataaactca ggcagtaatt cacgaatttt atcggctcct gctttatgcc cttcagattt tgttaagtta tgctgatctg cccaacgagc attaccgtcc attattattg ctaaatgttt tatgttagtc attattttat attttagatg ttgctgcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMQ QHLKYKIMTN IKHLAIIMDG NARWADQHNL TKSEGHKAGA DKIRELLPEF INLNIPYITL YTFSSENWQR SSTEVDFLIK LLSIYFKNEL NSLHKNGVKI KVIGRLNLLS SSLQKQINNA IELTKNNNKI TLCIAFSYGS RQEIVDACTK IIASGKKEIS ESDIGHALYD PEMPDVDLLI RPGGVYRISN FLLWQAAYAE LYFSPKYWPA FNKDDIIEAI NDYSKRKRTF GKR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAGC AACATCTAAA ATATAAAATA ATGACTAACA TAAAACATTT AGCAATAATA ATGGACGGTA ATGCTCGTTG GGCAGATCAG CATAACTTAA CAAAATCTGA AGGGCATAAA GCAGGAGCCG ATAAAATTCG TGAATTACTG CCTGAGTTTA TTAATCTTAA TATACCTTAT ATAACTTTAT ATACTTTCTC TTCCGAAAAT TGGCAACGTT CAAGCACGGA GGTAGATTTC TTAATAAAAT TATTAAGTAT TTATTTTAAA AATGAATTAA ACAGTTTACA TAAAAACGGC GTTAAAATAA AAGTAATAGG TAGGCTTAAT TTATTAAGTA GCTCATTGCA AAAACAAATA AATAATGCTA TAGAATTAAC AAAAAATAAT AATAAAATAA CGCTTTGTAT AGCTTTTAGT TATGGAAGTC GTCAAGAAAT AGTTGATGCC TGCACAAAAA TTATTGCAAG CGGTAAAAAA GAAATAAGTG AAAGCGATAT AGGACACGCT TTATATGATC CTGAAATGCC GGATGTAGAT TTATTAATAA GACCGGGCGG GGTCTATCGC ATCAGTAATT TCTTGCTTTG GCAAGCAGCT TATGCTGAAT TATATTTCTC ACCCAAATAT TGGCCCGCTT TTAATAAAGA TGATATAATA GAAGCTATAA ATGATTACTC AAAAAGGAAA AGAACATTTG GCAAAAGATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT