RifeA.00893.a

Ferredoxin--NADP reductase

CENTER ID: RifeA.00893.a
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00893.a.B1.GE35866 Full length( RifeA.00893.a ) 1 335
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00893.a.B1.PS01670 Full length( RifeA.00893.a ) 1 335

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.842
RefSeq: YP_246716.1
UniProt: Q4ULM2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTHTTDVVII GTGPVGLFAV FQAGMLGMKC HIIDAQEIIG GQCITLYPEK PIYDIPAYPK IAAEELIKQL ELQAAPFKPV YHLNQQAIEL NKQDDFFEIK TSKNTLIKSK VIIIAAGAGS FGPNKPPLAN IEDFEGKSVF YFINDKSKFA GKNIVIAGGG DSAVDWAISL SEIANKIYLV HRRDKFTAAP ESVRQLRHIA ETGKIELVTG YQLNALDGNN SELQSVIVKD LQNNTRKLDA NILLPFFGLK QDLGSLANWG LNVKLHHIEV DNSYYQTNIE GIYAIGDIAH YAGKLKLILT GFAEAASSLH HAYSRVFDGK ALHFEYSTTK YGERK
NT Sequence
ATGACACATA CTACGGATGT TGTAATAATA GGCACCGGTC CGGTTGGATT ATTTGCAGTT TTTCAAGCTG GAATGCTTGG GATGAAGTGC CACATTATCG ATGCTCAAGA AATCATAGGA GGTCAGTGTA TTACTCTATA CCCTGAAAAG CCTATTTACG ATATACCGGC ATATCCTAAA ATTGCTGCAG AAGAGCTAAT AAAACAGTTA GAGCTTCAAG CAGCTCCTTT TAAGCCTGTA TATCACTTAA ATCAGCAAGC TATAGAGCTA AATAAACAAG ATGATTTTTT TGAAATTAAA ACCTCAAAAA ACACTCTTAT TAAAAGCAAA GTTATTATTA TTGCAGCAGG AGCAGGTTCA TTCGGTCCAA ATAAACCGCC TCTTGCAAAT ATTGAAGATT TTGAAGGCAA ATCGGTTTTT TATTTTATTA ATGATAAAAG CAAATTTGCA GGTAAAAATA TAGTTATAGC AGGCGGCGGG GATTCGGCTG TTGACTGGGC TATTTCTCTT TCGGAAATTG CAAATAAAAT ATATTTAGTG CATAGGCGAG ATAAATTTAC GGCAGCTCCT GAAAGCGTAA GGCAATTAAG GCATATTGCA GAAACCGGTA AAATCGAGTT AGTAACCGGT TATCAATTAA ATGCTTTAGA TGGTAATAAT AGTGAACTTC AATCGGTTAT AGTTAAAGAT CTACAAAATA ATACTCGCAA ATTAGATGCC AATATATTAC TACCGTTTTT TGGTTTAAAA CAAGATTTAG GTAGCTTAGC AAATTGGGGT TTAAATGTTA AGCTTCACCA TATTGAGGTA GATAATTCCT ATTATCAAAC TAATATAGAA GGTATTTACG CCATTGGCGA TATTGCTCAT TATGCCGGTA AGCTTAAGTT GATTCTTACG GGTTTTGCTG AAGCGGCAAG TAGCCTTCAT CATGCTTATA GTAGAGTATT TGACGGCAAG GCTCTACATT TTGAGTATTC TACCACTAAG TATGGGGAAA GGAAG
Details for RifeA.00893.a.B1.GE35866
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMT HTTDVVIIGT GPVGLFAVFQ AGMLGMKCHI IDAQEIIGGQ CITLYPEKPI YDIPAYPKIA AEELIKQLEL QAAPFKPVYH LNQQAIELNK QDDFFEIKTS KNTLIKSKVI IIAAGAGSFG PNKPPLANIE DFEGKSVFYF INDKSKFAGK NIVIAGGGDS AVDWAISLSE IANKIYLVHR RDKFTAAPES VRQLRHIAET GKIELVTGYQ LNALDGNNSE LQSVIVKDLQ NNTRKLDANI LLPFFGLKQD LGSLANWGLN VKLHHIEVDN SYYQTNIEGI YAIGDIAHYA GKLKLILTGF AEAASSLHHA YSRVFDGKAL HFEYSTTKYG
Validated NT Sequence
ttccccatac ttagtggtag aatactcaaa atgtagagcc ttgccgtcaa atactctact ataagcatga tgaaggctac ttgccgcttc agcaaaaccc gtaagaatca acttaagctt accggcataa tgagcaatat cgccaatggc gtaaatacct tctatattag tttgataata ggaattatct acctcaatat ggtgaagctt aacatttaaa ccccaatttg ctaagctacc taaatcttgt tttaaaccaa aaaacggtag taatatattg gcatctaatt tgcgagtatt attttgtaga tctttaacta taaccgattg aagttcacta ttattaccat ctaaagcatt taattgataa ccggttacta actcgatttt accggtttct gcaatatgcc ttaattgcct tacgctttca ggagctgccg taaatttatc tcgcctatgc actaaatata ttttatttgc aatttccgaa agagaaatag cccagtcaac agccgaatcc ccgccgcctg ctataactat atttttacct gcaaatttgc ttttatcatt aataaaataa aaaaccgatt tgccttcaaa atcttcaata tttgcaagag gcggtttatt tggaccgaat gaacctgctc ctgctgcaat aataataact ttgcttttaa taagagtgtt ttttgaggtt ttaatttcaa aaaaatcatc ttgtttattt agctctatag cttgctgatt taagtgatat acaggcttaa aaggagctgc ttgaagctct aactgtttta ttagctcttc tgcagcaatt ttaggatatg ccggtatatc gtaaataggc ttttcagggt atagagtaat acactgacct cctatgattt cttgagcatc gataatgtgg cacttcatcc caagcattcc agcttgaaaa actgcaaata atccaaccgg accggtgcct attattacaa catccgtagt atgtgtcata tggtggtggt ggtggtgagc catggtat
Expected Protein Sequence
MAHHHHHHMT HTTDVVIIGT GPVGLFAVFQ AGMLGMKCHI IDAQEIIGGQ CITLYPEKPI YDIPAYPKIA AEELIKQLEL QAAPFKPVYH LNQQAIELNK QDDFFEIKTS KNTLIKSKVI IIAAGAGSFG PNKPPLANIE DFEGKSVFYF INDKSKFAGK NIVIAGGGDS AVDWAISLSE IANKIYLVHR RDKFTAAPES VRQLRHIAET GKIELVTGYQ LNALDGNNSE LQSVIVKDLQ NNTRKLDANI LLPFFGLKQD LGSLANWGLN VKLHHIEVDN SYYQTNIEGI YAIGDIAHYA GKLKLILTGF AEAASSLHHA YSRVFDGKAL HFEYSTTKYG ERK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac atactacgga tgttgtaata ataggcaccg gtccggttgg attatttgca gtttttcaag ctggaatgct tgggatgaag tgccacatta tcgatgctca agaaatcata ggaggtcagt gtattactct ataccctgaa aagcctattt acgatatacc ggcatatcct aaaattgctg cagaagagct aataaaacag ttagagcttc aagcagctcc ttttaagcct gtatatcact taaatcagca agctatagag ctaaataaac aagatgattt ttttgaaatt aaaacctcaa aaaacactct tattaaaagc aaagttatta ttattgcagc aggagcaggt tcattcggtc caaataaacc gcctcttgca aatattgaag attttgaagg caaatcggtt ttttatttta ttaatgataa aagcaaattt gcaggtaaaa atatagttat agcaggcggc ggggattcgg ctgttgactg ggctatttct ctttcggaaa ttgcaaataa aatatattta gtgcataggc gagataaatt tacggcagct cctgaaagcg taaggcaatt aaggcatatt gcagaaaccg gtaaaatcga gttagtaacc ggttatcaat taaatgcttt agatggtaat aatagtgaac ttcaatcggt tatagttaaa gatctacaaa ataatactcg caaattagat gccaatatat tactaccgtt ttttggttta aaacaagatt taggtagctt agcaaattgg ggtttaaatg ttaagcttca ccatattgag gtagataatt cctattatca aactaatata gaaggtattt acgccattgg cgatattgct cattatgccg gtaagcttaa gttgattctt acgggttttg ctgaagcggc aagtagcctt catcatgctt atagtagagt atttgacggc aaggctctac attttgagta ttctaccact aagtatgggg aaaggaagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for RifeA.00893.a.B1.PS01670
PURIFICATION DATe: 10/30/2012
CONCENTRATION: 39.96mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT HTTDVVIIGT GPVGLFAVFQ AGMLGMKCHI IDAQEIIGGQ CITLYPEKPI YDIPAYPKIA AEELIKQLEL QAAPFKPVYH LNQQAIELNK QDDFFEIKTS KNTLIKSKVI IIAAGAGSFG PNKPPLANIE DFEGKSVFYF INDKSKFAGK NIVIAGGGDS AVDWAISLSE IANKIYLVHR RDKFTAAPES VRQLRHIAET GKIELVTGYQ LNALDGNNSE LQSVIVKDLQ NNTRKLDANI LLPFFGLKQD LGSLANWGLN VKLHHIEVDN SYYQTNIEGI YAIGDIAHYA GKLKLILTGF AEAASSLHHA YSRVFDGKAL HFEYSTTKYG
Validated NT Sequence
ttccccatac ttagtggtag aatactcaaa atgtagagcc ttgccgtcaa atactctact ataagcatga tgaaggctac ttgccgcttc agcaaaaccc gtaagaatca acttaagctt accggcataa tgagcaatat cgccaatggc gtaaatacct tctatattag tttgataata ggaattatct acctcaatat ggtgaagctt aacatttaaa ccccaatttg ctaagctacc taaatcttgt tttaaaccaa aaaacggtag taatatattg gcatctaatt tgcgagtatt attttgtaga tctttaacta taaccgattg aagttcacta ttattaccat ctaaagcatt taattgataa ccggttacta actcgatttt accggtttct gcaatatgcc ttaattgcct tacgctttca ggagctgccg taaatttatc tcgcctatgc actaaatata ttttatttgc aatttccgaa agagaaatag cccagtcaac agccgaatcc ccgccgcctg ctataactat atttttacct gcaaatttgc ttttatcatt aataaaataa aaaaccgatt tgccttcaaa atcttcaata tttgcaagag gcggtttatt tggaccgaat gaacctgctc ctgctgcaat aataataact ttgcttttaa taagagtgtt ttttgaggtt ttaatttcaa aaaaatcatc ttgtttattt agctctatag cttgctgatt taagtgatat acaggcttaa aaggagctgc ttgaagctct aactgtttta ttagctcttc tgcagcaatt ttaggatatg ccggtatatc gtaaataggc ttttcagggt atagagtaat acactgacct cctatgattt cttgagcatc gataatgtgg cacttcatcc caagcattcc agcttgaaaa actgcaaata atccaaccgg accggtgcct attattacaa catccgtagt atgtgtcata tggtggtggt ggtggtgagc catggtat
Expressed Protein Sequence
MAHHHHHHMT HTTDVVIIGT GPVGLFAVFQ AGMLGMKCHI IDAQEIIGGQ CITLYPEKPI YDIPAYPKIA AEELIKQLEL QAAPFKPVYH LNQQAIELNK QDDFFEIKTS KNTLIKSKVI IIAAGAGSFG PNKPPLANIE DFEGKSVFYF INDKSKFAGK NIVIAGGGDS AVDWAISLSE IANKIYLVHR RDKFTAAPES VRQLRHIAET GKIELVTGYQ LNALDGNNSE LQSVIVKDLQ NNTRKLDANI LLPFFGLKQD LGSLANWGLN VKLHHIEVDN SYYQTNIEGI YAIGDIAHYA GKLKLILTGF AEAASSLHHA YSRVFDGKAL HFEYSTTKYG ERK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAC ATACTACGGA TGTTGTAATA ATAGGCACCG GTCCGGTTGG ATTATTTGCA GTTTTTCAAG CTGGAATGCT TGGGATGAAG TGCCACATTA TCGATGCTCA AGAAATCATA GGAGGTCAGT GTATTACTCT ATACCCTGAA AAGCCTATTT ACGATATACC GGCATATCCT AAAATTGCTG CAGAAGAGCT AATAAAACAG TTAGAGCTTC AAGCAGCTCC TTTTAAGCCT GTATATCACT TAAATCAGCA AGCTATAGAG CTAAATAAAC AAGATGATTT TTTTGAAATT AAAACCTCAA AAAACACTCT TATTAAAAGC AAAGTTATTA TTATTGCAGC AGGAGCAGGT TCATTCGGTC CAAATAAACC GCCTCTTGCA AATATTGAAG ATTTTGAAGG CAAATCGGTT TTTTATTTTA TTAATGATAA AAGCAAATTT GCAGGTAAAA ATATAGTTAT AGCAGGCGGC GGGGATTCGG CTGTTGACTG GGCTATTTCT CTTTCGGAAA TTGCAAATAA AATATATTTA GTGCATAGGC GAGATAAATT TACGGCAGCT CCTGAAAGCG TAAGGCAATT AAGGCATATT GCAGAAACCG GTAAAATCGA GTTAGTAACC GGTTATCAAT TAAATGCTTT AGATGGTAAT AATAGTGAAC TTCAATCGGT TATAGTTAAA GATCTACAAA ATAATACTCG CAAATTAGAT GCCAATATAT TACTACCGTT TTTTGGTTTA AAACAAGATT TAGGTAGCTT AGCAAATTGG GGTTTAAATG TTAAGCTTCA CCATATTGAG GTAGATAATT CCTATTATCA AACTAATATA GAAGGTATTT ACGCCATTGG CGATATTGCT CATTATGCCG GTAAGCTTAA GTTGATTCTT ACGGGTTTTG CTGAAGCGGC AAGTAGCCTT CATCATGCTT ATAGTAGAGT ATTTGACGGC AAGGCTCTAC ATTTTGAGTA TTCTACCACT AAGTATGGGG AAAGGAAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT