SSGCID: Target Page

RifeA.00339.a

Alanine racemase

CENTER ID:	RifeA.00339.a
ORGANISM:	Rickettsia felis URRWXCal2
ASSOCIATED DISEASE:	Flea-borne spotted fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.00339.a.B1.GE35864	Full length( RifeA.00339.a )		1	462

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|315456.7.peg.91
RefSeq:	YP_246095.1
UniProt:	Q4UNC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLCTLEINL SAIKNNYLLL QDICKTSLVG AAVKANGYGL GAVQISKALI EENCRHFFVA SSEEGVNLRK ALASWHESVF RHCEKNYTVI RRSNPVKNSV SQNFFNYFSG LQQCFAPRND GSSIHATTPK ALDNDVNILV LNGVFEHDAL ELIEYNLTPV LNNLKQIEIW QKFSNLKNRL LPCYLHFNTG INRLGLTHNE IEQLINNRDL LKGLDLQYII SHLAVSEEID NPYNLEQLNR FKTYLQYFPN VKASLANSGG IFLGQDYHFD LARPGAALYG LNPVIDLSNN LSYKEEFEGD TERRTAAYIN VREDSSTGST YKLPLEGGYS RGLQNPVTLK APIIHLQNLT LDSHIGYNMT FTTERDSVIA TLPLGYADGF SRNFSNQGEV FINGRSVPIV GRISMDLINI DVTDLPPLDI FLGQEAEIIG NYCTPDKIAS IIGTIGYEVL TSLGSRYKRI YK

NT Sequence

ATGAGCTTGT GTACTCTAGA AATAAATCTA TCTGCAATAA AAAATAATTA TCTTTTATTA CAAGATATTT GCAAAACCTC ATTAGTCGGT GCTGCCGTTA AAGCTAACGG TTACGGTCTT GGAGCAGTGC AAATTTCCAA AGCTTTAATA GAAGAAAATT GTAGGCATTT TTTTGTGGCC TCAAGTGAAG AAGGAGTGAA TTTACGCAAA GCTCTAGCTT CGTGGCATGA ATCGGTTTTC CGTCATTGCG AGAAGAATTA CACAGTAATT CGACGAAGCA ATCCAGTAAA AAATTCTGTA AGTCAGAATT TTTTTAATTA TTTTTCTGGA TTGCAACAAT GCTTCGCTCC TCGCAATGAC GGCTCTAGTA TCCATGCAAC AACGCCCAAA GCACTAGATA ATGATGTTAA TATTTTAGTT CTCAATGGAG TTTTTGAACA TGATGCTTTA GAGCTTATAG AATATAATTT AACTCCTGTT CTAAATAACT TAAAACAAAT AGAAATTTGG CAAAAATTTA GTAATTTGAA AAACCGATTA TTGCCTTGTT ATTTACATTT CAATACGGGG ATTAATCGCC TTGGATTAAC TCATAATGAA ATAGAGCAGT TAATTAATAA TCGTGATTTA TTAAAAGGGC TAGATTTACA ATATATTATA AGCCATCTAG CTGTATCCGA GGAAATAGAT AATCCTTATA ATCTAGAGCA ATTAAACAGA TTTAAAACTT ATTTACAATA TTTCCCAAAC GTCAAAGCAA GCCTTGCTAA TTCCGGTGGC ATATTTTTAG GACAGGACTA TCATTTTGAT TTAGCAAGAC CAGGGGCTGC CTTATACGGG CTTAATCCTG TAATAGACCT CTCGAATAAC CTATCTTATA AAGAGGAATT TGAAGGAGAC ACGGAACGCA GAACCGCAGC GTACATAAAC GTACGTGAGG ATTCGAGTAC CGGATCGACG TATAAATTAC CTTTAGAAGG AGGTTATTCG AGAGGTCTAC AAAATCCCGT AACCTTAAAA GCTCCTATAA TTCATTTACA GAATTTGACA CTAGATAGCC ATATCGGTTA TAATATGACT TTTACAACTG AGCGTGATAG TGTTATTGCA ACATTACCGC TTGGTTATGC CGATGGGTTT AGTCGTAATT TTAGCAATCA AGGCGAAGTA TTTATTAATG GTCGCAGCGT TCCTATAGTA GGGCGAATAT CGATGGATTT GATAAATATC GATGTTACCG ATCTACCACC GCTTGATATT TTTCTAGGGC AGGAAGCGGA AATTATCGGA AATTATTGCA CGCCCGATAA AATAGCAAGT ATTATAGGTA CTATAGGGTA TGAGGTGTTG ACTAGTCTCG GTAGTAGGTA TAAAAGAATT TATAAG

Details for RifeA.00339.a.B1.GE35864

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	52kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	70

Validated AA Sequence

Validated NT Sequence

attttgntta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgagc ttgtgtactc tagaaataaa tctatctgca ataaaaaata attatctttt attacaagat atttgcaaaa cctcattagt cggtgctgcc gttaaagcta acggttacgg tcttggagca gtgcaaattt ccaaagcttt aatagaagaa aattgtaggc atttttttgt ggcctcaagt gaagaaggag tgaatttacg caaagctcta gcttcgtggc atgaatcggt tttccgtcat tgcgagaaga attacacagt aattcgacga agcaatccag taaaaaattc tgtaagtcag aattttttta attatttttc tggattgcaa caatgcttcg ctcctcgcaa tgacggctct agtatccatg caacaacgcc caaagcacta gataatgatg ttaatatttt agttctcaat ggagtttttg aacatgatgc tttagagctt atagaatata atttaactcc tgttctaaat aacttaaaac aaatagaaat ttggcaaaaa tttagtaatt tgaaaaaccg attattgcct tgttatttac atttcaatac ggggattaat cgccttggat taactcataa tgaaatagag cagttaatta ataatcgtga tttattaaaa gggctagatt tacaatatat tataagccat ctagctgtat ccgaggaaat agataatcct tataatctag agcaattaaa caganttaaa acttatttac aatatttccc aaacgtcaaa gcaagccttg ctaattccgg tggcatattt ttaggacagg actatcattt tgatttagca agaccagggg ctgccttata cgggcttaat cctgtaatag anctctcgaa taacctatcn tataaagann annttgaagg anannnggnn ngcagaanng cnncgtacat aaacgtacgt gangnttcna gtaccngnat cgacgtataa nnnacntttn nnnantcnan ngnnnnnnaa anncngtanc nnaaagcnnc tnnannnttn cngnantnga nnctagnnnn ncnnnntnnn nnnnnngann ttnnnnnnnn nnnnnnnnan nnnncnnnnn nnnnnncnnn nnnnnnnnnc nnntnnnnna nncnannnnn nnnnnntnnn nnnnnngg

Expected Protein Sequence

MAHHHHHHMS LCTLEINLSA IKNNYLLLQD ICKTSLVGAA VKANGYGLGA VQISKALIEE NCRHFFVASS EEGVNLRKAL ASWHESVFRH CEKNYTVIRR SNPVKNSVSQ NFFNYFSGLQ QCFAPRNDGS SIHATTPKAL DNDVNILVLN GVFEHDALEL IEYNLTPVLN NLKQIEIWQK FSNLKNRLLP CYLHFNTGIN RLGLTHNEIE QLINNRDLLK GLDLQYIISH LAVSEEIDNP YNLEQLNRFK TYLQYFPNVK ASLANSGGIF LGQDYHFDLA RPGAALYGLN PVIDLSNNLS YKEEFEGDTE RRTAAYINVR EDSSTGSTYK LPLEGGYSRG LQNPVTLKAP IIHLQNLTLD SHIGYNMTFT TERDSVIATL PLGYADGFSR NFSNQGEVFI NGRSVPIVGR ISMDLINIDV TDLPPLDIFL GQEAEIIGNY CTPDKIASII GTIGYEVLTS LGSRYKRIYK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct tgtgtactct agaaataaat ctatctgcaa taaaaaataa ttatctttta ttacaagata tttgcaaaac ctcattagtc ggtgctgccg ttaaagctaa cggttacggt cttggagcag tgcaaatttc caaagcttta atagaagaaa attgtaggca tttttttgtg gcctcaagtg aagaaggagt gaatttacgc aaagctctag cttcgtggca tgaatcggtt ttccgtcatt gcgagaagaa ttacacagta attcgacgaa gcaatccagt aaaaaattct gtaagtcaga atttttttaa ttatttttct ggattgcaac aatgcttcgc tcctcgcaat gacggctcta gtatccatgc aacaacgccc aaagcactag ataatgatgt taatatttta gttctcaatg gagtttttga acatgatgct ttagagctta tagaatataa tttaactcct gttctaaata acttaaaaca aatagaaatt tggcaaaaat ttagtaattt gaaaaaccga ttattgcctt gttatttaca tttcaatacg gggattaatc gccttggatt aactcataat gaaatagagc agttaattaa taatcgtgat ttattaaaag ggctagattt acaatatatt ataagccatc tagctgtatc cgaggaaata gataatcctt ataatctaga gcaattaaac agatttaaaa cttatttaca atatttccca aacgtcaaag caagccttgc taattccggt ggcatatttt taggacagga ctatcatttt gatttagcaa gaccaggggc tgccttatac gggcttaatc ctgtaataga cctctcgaat aacctatctt ataaagagga atttgaagga gacacggaac gcagaaccgc agcgtacata aacgtacgtg aggattcgag taccggatcg acgtataaat tacctttaga aggaggttat tcgagaggtc tacaaaatcc cgtaacctta aaagctccta taattcattt acagaatttg acactagata gccatatcgg ttataatatg acttttacaa ctgagcgtga tagtgttatt gcaacattac cgcttggtta tgccgatggg tttagtcgta attttagcaa tcaaggcgaa gtatttatta atggtcgcag cgttcctata gtagggcgaa tatcgatgga tttgataaat atcgatgtta ccgatctacc accgcttgat atttttctag ggcaggaagc ggaaattatc ggaaattatt gcacgcccga taaaatagca agtattatag gtactatagg gtatgaggtg ttgactagtc tcggtagtag gtataaaaga atttataagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat