RifeA.00170.a
Acetoacetyl-CoA reductase
| CENTER ID: | RifeA.00170.a |
| ORGANISM: | Rickettsia felis URRWXCal2 |
| ASSOCIATED DISEASE: | Flea-borne spotted fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RifeA.00170.a.B1.GE35862 | Full length( RifeA.00170.a ) | 1 | 241 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RifeA.00170.a.B1.PS01660 | Full length( RifeA.00170.a ) | 1 | 241 |
Structures
4KMS
DEPOSITED: 5/8/2013
DETERMINATION: XRay
CLONE: RifeA.00170.a.B1.GE35862
PROTEIN: RifeA.00170.a.B1.PS01660
Publications by SSGCID
Crystal structure of Acetoacetyl-CoA reductase from Rickettsia felis
Abendroth JA, Davies DR, Edwards TE, Lorimer DD, Lukacs CM, Myler PJ, Staker BL, Zhang S, McLaughlin KJ, Rodarte JV
Acta Crystallogr F Struct Biol Commun - 2021
volume 77, issue Pt 2, pages 54-60
PMID: 33620038; PMCID: PMC7900926
PDB:
4KMS
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126618 |
| PATRIC ID: | fig|315456.7.peg.173 |
| RefSeq: | YP_246169.1 |
| UniProt: | Q4UN54 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEIAIVTGG TRGIGKATAL ELKNKGLTVV ANFFSNYDAA KEMEEKYGIK TKCWNVADFE ECRQAVKEIE EEFKKPVSIL VNNAGITKDK MLHRMSHQDW NDVINVNLNS CFNMSSSVME QMRNQDYGRI VNISSINAQA GQVGQTNYSA AKAGIIGFTK ALARETASKN ITVNCIAPGY IATEMVGAVP EDVLAKIINS IPKKRLGQPE EIARAVAFLV DENAGFITGE TISINGGHNM I
NT Sequence
ATGTCAGAAA TTGCAATTGT AACAGGCGGT ACTAGAGGAA TAGGTAAGGC TACCGCTTTA GAATTAAAAA ATAAGGGTCT TACGGTAGTT GCTAATTTTT TCAGTAATTA CGACGCTGCT AAAGAAATGG AAGAAAAATA CGGTATCAAA ACTAAATGCT GGAATGTTGC GGATTTTGAA GAATGCAGGC AAGCAGTAAA GGAAATTGAA GAGGAATTTA AAAAACCGGT AAGTATTCTT GTTAACAATG CAGGTATTAC CAAAGATAAA ATGCTACATA GAATGAGCCA TCAAGATTGG AATGATGTCA TTAACGTTAA TCTCAATTCT TGCTTTAATA TGTCTAGTAG CGTAATGGAA CAGATGCGAA ACCAAGATTA CGGTCGTATA GTAAATATCA GTTCAATTAA TGCTCAAGCA GGGCAGGTTG GACAAACTAA TTACTCTGCC GCTAAAGCCG GAATTATCGG CTTTACTAAG GCACTTGCTC GTGAAACTGC TTCTAAAAAT ATCACCGTTA ACTGCATAGC TCCAGGGTAT ATTGCAACCG AAATGGTAGG AGCAGTACCT GAAGACGTAC TTGCTAAAAT TATTAATAGT ATCCCCAAAA AAAGACTAGG ACAGCCTGAA GAAATAGCTA GAGCCGTAGC ATTTTTAGTT GATGAAAATG CCGGCTTTAT AACAGGTGAG ACTATTTCTA TCAACGGCGG CCATAATATG ATT
Details for RifeA.00170.a.B1.GE35862
| HARVESTED ON: | 8/14/2012 |
| SEQUENCED ON: | 8/18/2012 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS EIAIVTGGTR GIGKATALEL KNKGLTVVAN FFSNYDAAKE MEEKYGIKTK CWNVADFEEC RQAVKEIEEE FKKPVSILVN NAGITKDKML HRMSHQDWND VINVNLNSCF NMSSSVMEQM RNQDYGRIVN ISSINAQAGQ VGQTNYSAAK AGIIGFTKAL ARETASKNIT VNCIAPGYIA TEMVGAVPED VLAKIINSIP KKRLGQPEEI ARAVAFLVDE NAGFITGETI SINGGHNMI
Validated NT Sequence
ctcacttaaa tcatattatg gccgccgttg atagaaatag tctcacctgt tataaagccg gcattttcat caactaaaaa tgctacggct ctagctattt cttcaggctg tcctagtctt tttttgggga tactattaat aattttagca agtacgtctt caggtactgc tcctaccatt tcggttgcaa tataccctgg agctatgcag ttaacggtga tatttttaga agcagtttca cgagcaagtg ccttagtaaa gccgataatt ccggctttag cggcagagta attagtttgt ccaacctgcc ctgcttgagc attaattgaa ctgatattta ctatacgacc gtaatcttgg tttcgcatct gttccattac gctactagac atattaaagc aagaattgag attaacgtta atgacatcat tccaatcttg atggctcatt ctatgtagca ttttatcttt ggtaatacct gcattgttaa caagaatact taccggtttt ttaaattcct cttcaatttc ctttactgct tgcctgcatt cttcaaaatc cgcaacattc cagcatttag ttttgatacc gtatttttct tccatttctt tagcagcgtc gtaattactg aaaaaattag caactaccgt aagaccctta ttttttaatt ctaaagcggt agccttacct attcctctag taccgcctgt tacaattgca atttctgaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS EIAIVTGGTR GIGKATALEL KNKGLTVVAN FFSNYDAAKE MEEKYGIKTK CWNVADFEEC RQAVKEIEEE FKKPVSILVN NAGITKDKML HRMSHQDWND VINVNLNSCF NMSSSVMEQM RNQDYGRIVN ISSINAQAGQ VGQTNYSAAK AGIIGFTKAL ARETASKNIT VNCIAPGYIA TEMVGAVPED VLAKIINSIP KKRLGQPEEI ARAVAFLVDE NAGFITGETI SINGGHNMI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag aaattgcaat tgtaacaggc ggtactagag gaataggtaa ggctaccgct ttagaattaa aaaataaggg tcttacggta gttgctaatt ttttcagtaa ttacgacgct gctaaagaaa tggaagaaaa atacggtatc aaaactaaat gctggaatgt tgcggatttt gaagaatgca ggcaagcagt aaaggaaatt gaagaggaat ttaaaaaacc ggtaagtatt cttgttaaca atgcaggtat taccaaagat aaaatgctac atagaatgag ccatcaagat tggaatgatg tcattaacgt taatctcaat tcttgcttta atatgtctag tagcgtaatg gaacagatgc gaaaccaaga ttacggtcgt atagtaaata tcagttcaat taatgctcaa gcagggcagg ttggacaaac taattactct gccgctaaag ccggaattat cggctttact aaggcacttg ctcgtgaaac tgcttctaaa aatatcaccg ttaactgcat agctccaggg tatattgcaa ccgaaatggt aggagcagta cctgaagacg tacttgctaa aattattaat agtatcccca aaaaaagact aggacagcct gaagaaatag ctagagccgt agcattttta gttgatgaaa atgccggctt tataacaggt gagactattt ctatcaacgg cggccataat atgatttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for RifeA.00170.a.B1.PS01660
| PURIFICATION DATe: | 10/26/2012 |
| CONCENTRATION: | 69.7mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS EIAIVTGGTR GIGKATALEL KNKGLTVVAN FFSNYDAAKE MEEKYGIKTK CWNVADFEEC RQAVKEIEEE FKKPVSILVN NAGITKDKML HRMSHQDWND VINVNLNSCF NMSSSVMEQM RNQDYGRIVN ISSINAQAGQ VGQTNYSAAK AGIIGFTKAL ARETASKNIT VNCIAPGYIA TEMVGAVPED VLAKIINSIP KKRLGQPEEI ARAVAFLVDE NAGFITGETI SINGGHNMI
Validated NT Sequence
ctcacttaaa tcatattatg gccgccgttg atagaaatag tctcacctgt tataaagccg gcattttcat caactaaaaa tgctacggct ctagctattt cttcaggctg tcctagtctt tttttgggga tactattaat aattttagca agtacgtctt caggtactgc tcctaccatt tcggttgcaa tataccctgg agctatgcag ttaacggtga tatttttaga agcagtttca cgagcaagtg ccttagtaaa gccgataatt ccggctttag cggcagagta attagtttgt ccaacctgcc ctgcttgagc attaattgaa ctgatattta ctatacgacc gtaatcttgg tttcgcatct gttccattac gctactagac atattaaagc aagaattgag attaacgtta atgacatcat tccaatcttg atggctcatt ctatgtagca ttttatcttt ggtaatacct gcattgttaa caagaatact taccggtttt ttaaattcct cttcaatttc ctttactgct tgcctgcatt cttcaaaatc cgcaacattc cagcatttag ttttgatacc gtatttttct tccatttctt tagcagcgtc gtaattactg aaaaaattag caactaccgt aagaccctta ttttttaatt ctaaagcggt agccttacct attcctctag taccgcctgt tacaattgca atttctgaca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMS EIAIVTGGTR GIGKATALEL KNKGLTVVAN FFSNYDAAKE MEEKYGIKTK CWNVADFEEC RQAVKEIEEE FKKPVSILVN NAGITKDKML HRMSHQDWND VINVNLNSCF NMSSSVMEQM RNQDYGRIVN ISSINAQAGQ VGQTNYSAAK AGIIGFTKAL ARETASKNIT VNCIAPGYIA TEMVGAVPED VLAKIINSIP KKRLGQPEEI ARAVAFLVDE NAGFITGETI SINGGHNMI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAG AAATTGCAAT TGTAACAGGC GGTACTAGAG GAATAGGTAA GGCTACCGCT TTAGAATTAA AAAATAAGGG TCTTACGGTA GTTGCTAATT TTTTCAGTAA TTACGACGCT GCTAAAGAAA TGGAAGAAAA ATACGGTATC AAAACTAAAT GCTGGAATGT TGCGGATTTT GAAGAATGCA GGCAAGCAGT AAAGGAAATT GAAGAGGAAT TTAAAAAACC GGTAAGTATT CTTGTTAACA ATGCAGGTAT TACCAAAGAT AAAATGCTAC ATAGAATGAG CCATCAAGAT TGGAATGATG TCATTAACGT TAATCTCAAT TCTTGCTTTA ATATGTCTAG TAGCGTAATG GAACAGATGC GAAACCAAGA TTACGGTCGT ATAGTAAATA TCAGTTCAAT TAATGCTCAA GCAGGGCAGG TTGGACAAAC TAATTACTCT GCCGCTAAAG CCGGAATTAT CGGCTTTACT AAGGCACTTG CTCGTGAAAC TGCTTCTAAA AATATCACCG TTAACTGCAT AGCTCCAGGG TATATTGCAA CCGAAATGGT AGGAGCAGTA CCTGAAGACG TACTTGCTAA AATTATTAAT AGTATCCCCA AAAAAAGACT AGGACAGCCT GAAGAAATAG CTAGAGCCGT AGCATTTTTA GTTGATGAAA ATGCCGGCTT TATAACAGGT GAGACTATTT CTATCAACGG CGGCCATAAT ATGATTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T