SSGCID: Target Page

RifeA.00169.a

Replicative DNA helicase

CENTER ID:	RifeA.00169.a
ORGANISM:	Rickettsia felis URRWXCal2
ASSOCIATED DISEASE:	Flea-borne spotted fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.00169.a.B1.GE35861	Full length( RifeA.00169.a )		1	494

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|315456.7.peg.1034
RefSeq:	YP_246876.1
UniProt:	Q4UL62

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARNKINNDK NIAANEEADI PIPRVLPSNV QAEQMLLGAI LTNNELLSYV SEFLRSEHFF EPIHQKIYNA IEKITEKGLI ATPITLRSML TQDELFQEIE GAEYLAKLIT MSMMVINPVD YGKIIYDLAI KRNLINIGEE VVNDAYNSSL EIEAREQIEH AEAKLYDLAS ESLNEKSFTR IGIPISESLA SINRAMKNND HVIGISTGLL DLDNKLFGFH NSDLLILAGR PSMGKTAFAI NLALNACNNM RLKNIRDNQE IQSVGFFSLE MSSEQLTTRL LSMCAEIDST SLRTGILGEE KYNRLRKEAN TLSELQFFID DTPALSISAI RTRARRMKRK HNLGILFIDY LQLIRGVSKS ENRVSEISEI TQGLKAIAKE LNIPVIALSQ LSRAVELRED KKPMLSDLRE SGTIEQDADI VMFIYREEYY LTRKEPAAGD AKHAEWLDKL NKVYNIADII VAKHRNGPVG NVPLYYDSQF SKFGNLEKRT FSSN

NT Sequence

ATGGCACGTA ATAAAATAAA TAATGACAAA AACATAGCTG CTAACGAAGA AGCAGATATT CCTATCCCAA GGGTTTTACC TTCAAATGTT CAAGCAGAAC AAATGCTACT TGGAGCAATT CTAACTAATA ATGAATTACT TAGTTATGTA TCGGAATTTT TGCGTAGTGA ACATTTTTTC GAACCTATTC ATCAAAAAAT CTATAACGCA ATTGAAAAAA TTACCGAAAA AGGTTTGATA GCTACACCTA TTACTTTACG TAGTATGTTA ACTCAAGATG AACTATTTCA AGAAATAGAA GGAGCGGAAT ATTTAGCAAA ATTGATAACT ATGTCAATGA TGGTAATAAA TCCGGTTGAT TACGGTAAAA TAATATATGA TTTAGCAATA AAGCGTAATT TAATCAATAT CGGTGAAGAA GTAGTAAACG ACGCTTATAA CTCCTCATTA GAGATTGAAG CAAGAGAACA GATTGAACAT GCCGAGGCTA AACTTTACGA TTTAGCTAGC GAAAGCCTAA ATGAAAAGAG TTTTACCAGA ATCGGTATTC CTATTTCGGA ATCCCTTGCT AGCATCAATA GAGCTATGAA AAATAACGAT CACGTAATCG GTATATCTAC AGGTTTACTT GATCTAGATA ATAAATTATT CGGCTTTCAT AATTCCGATC TTTTAATTCT CGCAGGACGT CCATCGATGG GTAAAACAGC ATTTGCTATA AATCTTGCAC TTAACGCCTG TAATAATATG CGTCTTAAAA ATATTAGGGA TAATCAGGAA ATTCAGTCGG TAGGTTTTTT CTCCTTAGAA ATGTCCTCAG AACAGCTAAC CACACGTCTA CTTTCAATGT GTGCAGAAAT TGATTCTACT TCTCTTCGTA CGGGGATTCT AGGTGAAGAA AAATATAACC GTCTTCGCAA AGAAGCAAAT ACTTTATCGG AATTACAATT TTTTATCGAT GATACCCCTG CTCTATCTAT TTCCGCTATA AGAACAAGAG CTAGAAGAAT GAAACGTAAA CATAATCTCG GTATATTATT TATCGATTAT TTACAATTAA TTAGAGGAGT AAGTAAATCT GAAAATAGAG TTAGCGAGAT TTCAGAAATA ACCCAAGGTT TAAAGGCAAT AGCAAAAGAG TTAAATATTC CAGTTATTGC GTTATCTCAG CTTTCAAGAG CGGTAGAACT ACGTGAGGAT AAAAAACCTA TGCTATCCGA CCTTAGAGAA TCAGGAACTA TAGAACAGGA TGCTGATATA GTAATGTTTA TTTATCGTGA AGAATATTAT TTAACAAGAA AAGAACCGGC AGCAGGCGAT GCTAAACATG CCGAATGGTT AGATAAGCTC AATAAAGTAT ATAATATTGC CGATATAATT GTTGCAAAAC ATCGTAACGG ACCGGTTGGA AATGTTCCGC TTTATTATGA TAGTCAATTT TCTAAATTTG GTAATTTGGA GAAAAGAACT TTTAGTTCTA AT

Details for RifeA.00169.a.B1.GE35861

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	90
PERCENT COVERAGE:	69

Validated AA Sequence

Validated NT Sequence

attttgttta ctttnagaag gaganatacc atggctcacc accaccacca ccatatggca cgtaataaaa taaataatga caaaaacata gctgctaacg aagaagcaga tattcctatc ccaagggttt taccttcaaa tgttcaagca gaacaaatgc tacttggagc aattctaact aataatgaat tacttagtta tgtatcggaa tttttgcgta gtgaacattt tttcgaacct attcatcaaa aaatctataa cgcaattgaa aaaattaccg aaaaaggttt gatagctaca cctattactt tacgtagtat gttaactcaa gatgaactat ttcaagaaat agaaggagcg gaatatttag caaaattgat aactatgtca atgatggtaa taaatccggt tgattacggt aaaataatat atgatttagc aataaagcgt aatttaatca atatcggtga agaagtagta aacgacgctt ataactcctc attagagatt gaagcaagag aacagattga acatgccgag gctaaacttt acgatttagc tagcgaaagc ctaaatgaaa agagttttac cagaatcggt attcctattt cggaatccct tgctagcatc aatagagcta tgaaaaataa cgatcacgta atcggtatat ctacaggttt acttgatcta gataataaat tattcggctt tcataattcc gatcttttaa ttctcgcang acgtccatcg atgggtaaaa cagcatttgc tataaatctt gcacttaacg cctgtaataa tatgcgtctt aaaaatatta gggataatca ggaaattcag tcggtaggtt ttttctcctt agaaatgtcc tcagaacagc tnaccacacg tctactttca atgtgtgcag aaattgattc tacttctctt cgtacgggga ttctaggnga agaaaaatat aannngtctt cgcaaagaag caaanncttt atcggaattn nnattttnnn tcgatgnnnn ccnngctnnn nctnnttnnn tnnnnagaac nagnnctnnn annatgnaan ntaannnnnn tcggnnnnnn tnnttatcga tnnnttnnnn nnnntnnnnn gnannngtaa nnctnnaaaa nnnnnnnnnn nnnnnnnntn nnnnaannan nnntnnnngn nannnnnnna nnnnnnnnnn tnnnnnnnnn cnttnnnnnn nnnnnnnnnn a

Expected Protein Sequence

MAHHHHHHMA RNKINNDKNI AANEEADIPI PRVLPSNVQA EQMLLGAILT NNELLSYVSE FLRSEHFFEP IHQKIYNAIE KITEKGLIAT PITLRSMLTQ DELFQEIEGA EYLAKLITMS MMVINPVDYG KIIYDLAIKR NLINIGEEVV NDAYNSSLEI EAREQIEHAE AKLYDLASES LNEKSFTRIG IPISESLASI NRAMKNNDHV IGISTGLLDL DNKLFGFHNS DLLILAGRPS MGKTAFAINL ALNACNNMRL KNIRDNQEIQ SVGFFSLEMS SEQLTTRLLS MCAEIDSTSL RTGILGEEKY NRLRKEANTL SELQFFIDDT PALSISAIRT RARRMKRKHN LGILFIDYLQ LIRGVSKSEN RVSEISEITQ GLKAIAKELN IPVIALSQLS RAVELREDKK PMLSDLRESG TIEQDADIVM FIYREEYYLT RKEPAAGDAK HAEWLDKLNK VYNIADIIVA KHRNGPVGNV PLYYDSQFSK FGNLEKRTFS SN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac gtaataaaat aaataatgac aaaaacatag ctgctaacga agaagcagat attcctatcc caagggtttt accttcaaat gttcaagcag aacaaatgct acttggagca attctaacta ataatgaatt acttagttat gtatcggaat ttttgcgtag tgaacatttt ttcgaaccta ttcatcaaaa aatctataac gcaattgaaa aaattaccga aaaaggtttg atagctacac ctattacttt acgtagtatg ttaactcaag atgaactatt tcaagaaata gaaggagcgg aatatttagc aaaattgata actatgtcaa tgatggtaat aaatccggtt gattacggta aaataatata tgatttagca ataaagcgta atttaatcaa tatcggtgaa gaagtagtaa acgacgctta taactcctca ttagagattg aagcaagaga acagattgaa catgccgagg ctaaacttta cgatttagct agcgaaagcc taaatgaaaa gagttttacc agaatcggta ttcctatttc ggaatccctt gctagcatca atagagctat gaaaaataac gatcacgtaa tcggtatatc tacaggttta cttgatctag ataataaatt attcggcttt cataattccg atcttttaat tctcgcagga cgtccatcga tgggtaaaac agcatttgct ataaatcttg cacttaacgc ctgtaataat atgcgtctta aaaatattag ggataatcag gaaattcagt cggtaggttt tttctcctta gaaatgtcct cagaacagct aaccacacgt ctactttcaa tgtgtgcaga aattgattct acttctcttc gtacggggat tctaggtgaa gaaaaatata accgtcttcg caaagaagca aatactttat cggaattaca attttttatc gatgataccc ctgctctatc tatttccgct ataagaacaa gagctagaag aatgaaacgt aaacataatc tcggtatatt atttatcgat tatttacaat taattagagg agtaagtaaa tctgaaaata gagttagcga gatttcagaa ataacccaag gtttaaaggc aatagcaaaa gagttaaata ttccagttat tgcgttatct cagctttcaa gagcggtaga actacgtgag gataaaaaac ctatgctatc cgaccttaga gaatcaggaa ctatagaaca ggatgctgat atagtaatgt ttatttatcg tgaagaatat tatttaacaa gaaaagaacc ggcagcaggc gatgctaaac atgccgaatg gttagataag ctcaataaag tatataatat tgccgatata attgttgcaa aacatcgtaa cggaccggtt ggaaatgttc cgctttatta tgatagtcaa ttttctaaat ttggtaattt ggagaaaaga acttttagtt ctaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat