RifeA.00155.a

Probable DNA helicase II homolog

CENTER ID: RifeA.00155.a
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00155.a.B1.GE35857 Full length( RifeA.00155.a ) 1 654
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.825
RefSeq: YP_246703.1
UniProt: Q4ULN5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNNQDFIHT LNPQQQKAVL HTKGPLLLLA GAGTGKTKVL TSRIANIVHQ NLASPQNILA VTFTNKAAKE MAERVNSLIN CYGLNIGTFH SMAARILRDQ IEHLNLGLNN RFTIISHDDQ LKLVKDIVKL KDIDTKKYAP KLIHIIISRW KDQGLLPSKL SASDTNLPLQ RVAKLVYEEY QKNLLISNVL DFGDLLLYNN ELFIKNPEIL RYYQEKYRYI LIDEYQDTNV VQYLWARMLA SLYKNICCVG DDDQSIYGWR GAEVGNILRF EKDFAGATII KLEQNYRSTL PILAAASNVI NNNKNRHGKT LWTDRESGEK IKIISCWSDK EEARYIAGEI DKLVREDRYN AGNIAILVRA GFQTRSFEEA FINSAMPYKI IGGLRFYERM EIRDVLAYIR ISLNQNDNLA LERIINVPKR AIGAASLNKI RVYALERNIS NFAAIKEMLE MGEIKAKPYE TLKDLVTKID NWHERFSIDA PINVVKAILD DSGYLEMLQE EKTEEAFGRI ENINEMLRAI AEFNDIHDFI EHSSLVMENE VLETNYGGSV TIMTLHAAKG LEFDVVFLPG WEEGVFPSQR SLDEEGEKGL EEERRIAYVG ITRAKKDLYI THAESRKIFY EIVRSYPSRF ITEIPDEITI RTSSMKKYNS FYKF
NT Sequence
ATGTTAAATA ATCAAGATTT CATACATACA TTAAATCCAC AACAGCAAAA AGCAGTTCTT CATACTAAAG GACCGCTTTT ATTGCTTGCA GGTGCAGGGA CAGGTAAAAC AAAAGTACTT ACCTCACGAA TAGCTAATAT TGTTCACCAA AATTTAGCGT CACCTCAAAA TATTCTAGCC GTTACTTTTA CTAATAAAGC TGCTAAAGAA ATGGCTGAAA GAGTTAACAG TCTAATTAAT TGCTACGGTC TTAATATAGG CACTTTCCAC TCAATGGCAG CTAGGATATT ACGTGACCAA ATTGAGCATT TAAATCTTGG TTTAAATAAT AGATTTACTA TTATTAGTCA TGATGATCAA TTAAAACTAG TTAAAGATAT AGTAAAACTA AAAGATATCG ATACTAAAAA ATATGCTCCT AAATTAATAC ATATCATAAT TTCGAGATGG AAAGATCAAG GATTATTACC AAGTAAACTA TCAGCATCAG ATACTAATTT ACCGTTGCAG AGGGTGGCAA AACTTGTATA CGAAGAATAT CAGAAAAATT TACTTATTTC TAATGTTTTA GATTTTGGAG ATTTGCTACT TTATAATAAT GAGCTTTTTA TTAAAAATCC CGAAATACTA AGATATTATC AGGAAAAATA TCGATATATT TTAATCGATG AATATCAAGA CACTAACGTT GTCCAGTATT TATGGGCAAG AATGCTCGCA AGTTTATATA AAAATATTTG CTGTGTAGGG GATGATGATC AATCTATCTA TGGTTGGCGA GGAGCGGAAG TCGGTAATAT ATTACGCTTT GAAAAAGATT TTGCAGGTGC AACGATTATT AAATTAGAGC AGAATTATAG ATCAACTTTG CCTATACTTG CTGCTGCTTC CAATGTTATT AATAATAATA AAAACCGTCA CGGTAAAACG TTATGGACGG ATAGGGAAAG CGGTGAAAAG ATAAAAATTA TCTCTTGTTG GAGTGATAAA GAAGAAGCAA GATATATAGC CGGCGAAATA GATAAATTAG TGAGAGAAGA CCGATATAAT GCCGGCAATA TTGCAATATT AGTACGAGCC GGATTTCAAA CAAGAAGTTT TGAGGAAGCG TTTATAAATA GTGCTATGCC TTATAAAATT ATAGGAGGTT TGCGGTTCTA TGAACGTATG GAAATAAGAG ACGTGCTTGC TTATATCCGT ATATCTTTAA ATCAAAATGA CAATCTAGCA CTTGAGCGTA TTATAAATGT GCCGAAAAGG GCAATAGGTG CAGCTAGCTT AAATAAAATT AGAGTCTATG CACTTGAGCG AAATATTTCT AATTTTGCAG CCATTAAAGA AATGTTGGAA ATGGGCGAGA TTAAAGCAAA ACCATATGAA ACTCTAAAAG ATTTGGTTAC TAAAATTGAT AATTGGCACG AAAGATTTAG TATAGATGCT CCAATAAACG TAGTTAAAGC AATACTTGAT GATTCCGGTT ATCTTGAAAT GCTTCAAGAA GAAAAAACCG AAGAAGCATT CGGTAGAATC GAAAATATTA ACGAAATGCT TAGAGCTATT GCCGAATTTA ACGATATTCA TGATTTTATC GAACATTCAA GCTTAGTTAT GGAAAACGAA GTGCTTGAAA CTAATTACGG TGGTTCTGTT ACTATAATGA CCCTGCACGC AGCTAAAGGG CTTGAGTTTG ACGTGGTGTT TTTACCGGGC TGGGAAGAGG GGGTATTTCC GTCTCAAAGG TCTCTAGACG AAGAAGGCGA AAAAGGCTTA GAAGAAGAAC GCCGCATTGC TTATGTCGGC ATTACGAGAG CCAAAAAAGA CCTCTACATT ACCCACGCCG AAAGCAGAAA AATATTTTAT GAAATAGTCC GCTCTTACCC TTCAAGGTTT ATAACCGAAA TCCCTGATGA AATTACGATA AGGACCTCTT CTATGAAAAA ATACAATTCT TTTTATAAGT TT
Details for RifeA.00155.a.B1.GE35857
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 76kDa
OBSERVED MW: 76kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 59
Validated AA Sequence
MAHHHHHHML NXXDFIHTLN PQQQKAVLHT KGPLLLLAGA GTGKTKVLTS RIANIVHQNL ASPQNILAVT FTNKAAKEMA ERVNSLINCY XLNIGTFHSM AARILRDQIE HLNLGLNNRF TIISHDDQLK LVKDIVKLKD IDTKKYAPKL IHIIISRWKD QGLLXXKLSA SDTNLPLQRV AKLVYEEYQK NLLISNVLDF GDLLLYNNEL FIKNPEILRY YQEKYRYILI DEYQDTNVVQ YLWARMLASL YKNICCVGDD DQSIYGWRGA EVGNILRFEK DFAGATIIKL EQNYRSTLPI LAAASXXIXN NKNRXGKTLW TDRXSGEXXX XSXGSDXXXX XXXXXXRXXX XXXXXXXXXA XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttttntttnc tttaagaagg aganatacca tggctcacca ccaccaccac catatgttaa atantcnaga tttcatacat acnttaaatc cacaacagca aaaagcagtt cttcatacta aaggaccgct tttattgctt gcaggtgcag ggacaggtaa aacaaaagta cttacctcac gaatagctaa tattgttcac caaaatttag cgtcacctca aaatattcta gccgttactt ttactaataa agctgctaaa gaaatggctg agagagttaa cagtctaatt aattgctacn gtcttaatat aggcactttc cactcaatgg cagctaggat attacgtgac caaattgagc atttaaatct tggtttaaat aatagattta ctattattag tcatgatgat caattaaaac tagttaaaga tatagtaaaa ctaaaagata tcgatactaa aaaatatgct cctaaattaa tacatatcat aatttcnaga tggaaagatc aaggattatt annnnnnaaa ctatcagcat cagatactaa tttaccgttg cagagggtgg caaaacttgt atacgaagaa tatcagaaaa atttacttat ttctaatgtt ttagattttg gagatttgct actttataat aatgagcttt ttattaaaaa tcccgaaata ctaagatatt atcaggaaaa atatcgatat attttaatcg atgaatatca agacactaac gttgtccagt atttatgggc aagaatgctc gcaagtttat ataaaaatat ttgctgtgta ggggatgatg atcaatctat ctatggttgg cgaggagcgg aagtcggtaa tatattacgc tttgaaaaag attttgcagg tgcaacgatt attaaattag agcagaatta tagatcaact ttgcctatac ttgctgctgc ttccnnngnt attantaata ataaaaaccg tcncggtaaa acnttatgga cggatagggn aagcggtgaa aannnaaant natctcnngg gagtgatnaa gannnancnn ganntntanc ngnnannaga naannnnngn nananancga nnnnngncnn nnnnnngcan tantnnnacn annnnntann nagnanntnn nnnnnnnnnn nannnnnncn ntnnntnnnn nnnnnnnnnn nannnnnnnn nnnnnnnnnn nngng
Expected Protein Sequence
MAHHHHHHML NNQDFIHTLN PQQQKAVLHT KGPLLLLAGA GTGKTKVLTS RIANIVHQNL ASPQNILAVT FTNKAAKEMA ERVNSLINCY GLNIGTFHSM AARILRDQIE HLNLGLNNRF TIISHDDQLK LVKDIVKLKD IDTKKYAPKL IHIIISRWKD QGLLPSKLSA SDTNLPLQRV AKLVYEEYQK NLLISNVLDF GDLLLYNNEL FIKNPEILRY YQEKYRYILI DEYQDTNVVQ YLWARMLASL YKNICCVGDD DQSIYGWRGA EVGNILRFEK DFAGATIIKL EQNYRSTLPI LAAASNVINN NKNRHGKTLW TDRESGEKIK IISCWSDKEE ARYIAGEIDK LVREDRYNAG NIAILVRAGF QTRSFEEAFI NSAMPYKIIG GLRFYERMEI RDVLAYIRIS LNQNDNLALE RIINVPKRAI GAASLNKIRV YALERNISNF AAIKEMLEMG EIKAKPYETL KDLVTKIDNW HERFSIDAPI NVVKAILDDS GYLEMLQEEK TEEAFGRIEN INEMLRAIAE FNDIHDFIEH SSLVMENEVL ETNYGGSVTI MTLHAAKGLE FDVVFLPGWE EGVFPSQRSL DEEGEKGLEE ERRIAYVGIT RAKKDLYITH AESRKIFYEI VRSYPSRFIT EIPDEITIRT SSMKKYNSFY KF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa ataatcaaga tttcatacat acattaaatc cacaacagca aaaagcagtt cttcatacta aaggaccgct tttattgctt gcaggtgcag ggacaggtaa aacaaaagta cttacctcac gaatagctaa tattgttcac caaaatttag cgtcacctca aaatattcta gccgttactt ttactaataa agctgctaaa gaaatggctg aaagagttaa cagtctaatt aattgctacg gtcttaatat aggcactttc cactcaatgg cagctaggat attacgtgac caaattgagc atttaaatct tggtttaaat aatagattta ctattattag tcatgatgat caattaaaac tagttaaaga tatagtaaaa ctaaaagata tcgatactaa aaaatatgct cctaaattaa tacatatcat aatttcgaga tggaaagatc aaggattatt accaagtaaa ctatcagcat cagatactaa tttaccgttg cagagggtgg caaaacttgt atacgaagaa tatcagaaaa atttacttat ttctaatgtt ttagattttg gagatttgct actttataat aatgagcttt ttattaaaaa tcccgaaata ctaagatatt atcaggaaaa atatcgatat attttaatcg atgaatatca agacactaac gttgtccagt atttatgggc aagaatgctc gcaagtttat ataaaaatat ttgctgtgta ggggatgatg atcaatctat ctatggttgg cgaggagcgg aagtcggtaa tatattacgc tttgaaaaag attttgcagg tgcaacgatt attaaattag agcagaatta tagatcaact ttgcctatac ttgctgctgc ttccaatgtt attaataata ataaaaaccg tcacggtaaa acgttatgga cggataggga aagcggtgaa aagataaaaa ttatctcttg ttggagtgat aaagaagaag caagatatat agccggcgaa atagataaat tagtgagaga agaccgatat aatgccggca atattgcaat attagtacga gccggatttc aaacaagaag ttttgaggaa gcgtttataa atagtgctat gccttataaa attataggag gtttgcggtt ctatgaacgt atggaaataa gagacgtgct tgcttatatc cgtatatctt taaatcaaaa tgacaatcta gcacttgagc gtattataaa tgtgccgaaa agggcaatag gtgcagctag cttaaataaa attagagtct atgcacttga gcgaaatatt tctaattttg cagccattaa agaaatgttg gaaatgggcg agattaaagc aaaaccatat gaaactctaa aagatttggt tactaaaatt gataattggc acgaaagatt tagtatagat gctccaataa acgtagttaa agcaatactt gatgattccg gttatcttga aatgcttcaa gaagaaaaaa ccgaagaagc attcggtaga atcgaaaata ttaacgaaat gcttagagct attgccgaat ttaacgatat tcatgatttt atcgaacatt caagcttagt tatggaaaac gaagtgcttg aaactaatta cggtggttct gttactataa tgaccctgca cgcagctaaa gggcttgagt ttgacgtggt gtttttaccg ggctgggaag agggggtatt tccgtctcaa aggtctctag acgaagaagg cgaaaaaggc ttagaagaag aacgccgcat tgcttatgtc ggcattacga gagccaaaaa agacctctac attacccacg ccgaaagcag aaaaatattt tatgaaatag tccgctctta cccttcaagg tttataaccg aaatccctga tgaaattacg ataaggacct cttctatgaa aaaatacaat tctttttata agttttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat