RifeA.00148.a

Tyrosine recombinase xerC

CENTER ID: RifeA.00148.a
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00148.a.B1.GE35855 Full length( RifeA.00148.a ) 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.1559
RefSeq: YP_247311.1
UniProt: Q4UJZ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLDTSIQELI DKWQKYLDLQ RNYSNHTVIS YNNDLKHFLE FMNYYNSELV TINHIKTADI RLIRSWLAKR NCDNFTTSSI SRGLSAVKNF YRFLEKTTQL NSHIIFSIKS PKKTKLLPKA LSEDDVVISL EHIEEYGNVK WVELRNKALL VLIYASGLRI SEALSITKLH LQNLEFIRII GKGSKERIIP WLPIAKNLIT QYLEILPYKL GDNEPIFRGK QGKKLQPPVF NRELIKLKHF YGLPQHLTAH SFRHSFASHL LEHGADLRSI QELLGHKSLS TTQNYTKTSI KHLEAVYTTA YPIKK
NT Sequence
ATGTTAGATA CATCAATTCA AGAATTAATA GATAAGTGGC AAAAATACCT CGATTTGCAA AGAAATTACT CTAATCATAC AGTAATTTCC TATAATAATG ACCTTAAGCA TTTCCTTGAG TTTATGAATT ATTATAATTC AGAGCTTGTA ACGATAAATC ATATTAAAAC CGCAGATATA AGATTAATCC GAAGTTGGCT TGCAAAACGT AACTGTGATA ATTTTACCAC TTCTTCAATT TCACGTGGTT TATCTGCAGT AAAAAATTTT TATAGGTTTT TAGAGAAAAC AACACAGTTA AATAGTCATA TAATTTTTTC TATAAAATCT CCTAAAAAAA CTAAATTATT ACCAAAAGCT TTATCGGAGG ATGATGTAGT AATATCACTT GAACATATTG AAGAATACGG GAATGTTAAG TGGGTAGAGC TTAGAAATAA AGCCCTACTT GTTCTTATAT ATGCTTCAGG TTTACGTATA TCGGAGGCAT TATCAATTAC GAAACTTCAT CTACAAAATC TAGAATTTAT AAGAATAATA GGTAAAGGTA GTAAAGAAAG AATAATTCCG TGGTTACCGA TTGCTAAAAA TTTAATTACC CAATATTTAG AAATATTGCC GTATAAGCTA GGTGATAATG AACCGATATT TAGAGGAAAG CAGGGTAAGA AATTGCAGCC ACCGGTATTT AATCGTGAAT TAATTAAATT AAAGCATTTT TATGGTTTAC CGCAGCATTT AACTGCTCAT TCATTTAGGC ATAGTTTTGC TTCGCATTTG CTTGAGCATG GTGCAGATTT ACGCTCTATT CAAGAGCTAT TAGGTCATAA AAGTCTATCT ACTACACAAA ACTATACTAA AACAAGTATA AAGCATTTAG AGGCTGTATA TACTACCGCA TATCCAATTA AAAAA
Details for RifeA.00148.a.B1.GE35855
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML DTSIQELIDK WQKYLDLQRN YSNHTVISYN NDLKHFLEFM NYYNSELVTI NHIKTADIRL IRSWLAKRNC DNFTTSSISR GLSAVKNFYR FLEKTTQLNS HIIFSIKSPK KTKLLPKALS EDDVVISLEH IEEYGNVKWV ELRNKALLVL IYASGLRISE ALSITKLHLQ NLEFIRIIGK GSKERIIPWL PIAKNLITQY LEILPYKLGD NEPIFRGKQG KKLQPPVFNR ELIKLKHFYG LPQHLTAHSF RHSFASHLLE HGAXLRSIQE LLXHKSLSTT QNYTKTSIKH LEAXYTTAYP XKK
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgtta gatacatcaa ttcaagaatt aatagataag tggcaaaaat acctcgattt gcaaagaaat tactctaatc atacagtaat ttcctataat aatgacctta agcatttcct tgagtttatg aattattata attcagagct tgtaacgata aatcatatta aaaccgcaga tataagatta atccgaagtt ggcttgcaaa acgtaactgt gataatttta ccacttcttc aatttcacgt ggtttatctg cagtaaaaaa tttttatagg tttttagaga aaacaacaca gttaaatagt catataattt tttctataaa atctcctaaa aaaactaaat tattaccaaa agctttatcg gaggatgatg tagtaatatc acttgaacat attgaagaat acgggaatgt taagtgggta gagcttagaa ataaagccct acttgttctt atatatgctt caggtttacg tatatcggag gcattatcaa ttacgaaact tcatctacaa aatctagaat ttataagaat aataggtaaa ggtagtaaag aaagaataat tccgtggtta ccgattgcta aaaatttaat tacccaatat ttagaaatat tgccgtataa gctaggtgat aatgaaccga tatttagagg aaagcagggt aagaaattgc agccaccggt atttaatcgt gaattaatta aattaaagca tttttatggn ttaccgcagc atttaactgc tcattcattt aggcatagtt ttgcttcgca tttgcttgag catggtgcag anttacgctc tattcaagag ctattanntc ataaaagtct atctactaca caaaactata ctaaaacaag tataaagcat ttagaggctg natatactac cgcatatccn nttaaaaaat aagtgannan naaggancnn nnnnnnnnnn cnncc
Expected Protein Sequence
MAHHHHHHML DTSIQELIDK WQKYLDLQRN YSNHTVISYN NDLKHFLEFM NYYNSELVTI NHIKTADIRL IRSWLAKRNC DNFTTSSISR GLSAVKNFYR FLEKTTQLNS HIIFSIKSPK KTKLLPKALS EDDVVISLEH IEEYGNVKWV ELRNKALLVL IYASGLRISE ALSITKLHLQ NLEFIRIIGK GSKERIIPWL PIAKNLITQY LEILPYKLGD NEPIFRGKQG KKLQPPVFNR ELIKLKHFYG LPQHLTAHSF RHSFASHLLE HGADLRSIQE LLGHKSLSTT QNYTKTSIKH LEAVYTTAYP IKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttag atacatcaat tcaagaatta atagataagt ggcaaaaata cctcgatttg caaagaaatt actctaatca tacagtaatt tcctataata atgaccttaa gcatttcctt gagtttatga attattataa ttcagagctt gtaacgataa atcatattaa aaccgcagat ataagattaa tccgaagttg gcttgcaaaa cgtaactgtg ataattttac cacttcttca atttcacgtg gtttatctgc agtaaaaaat ttttataggt ttttagagaa aacaacacag ttaaatagtc atataatttt ttctataaaa tctcctaaaa aaactaaatt attaccaaaa gctttatcgg aggatgatgt agtaatatca cttgaacata ttgaagaata cgggaatgtt aagtgggtag agcttagaaa taaagcccta cttgttctta tatatgcttc aggtttacgt atatcggagg cattatcaat tacgaaactt catctacaaa atctagaatt tataagaata ataggtaaag gtagtaaaga aagaataatt ccgtggttac cgattgctaa aaatttaatt acccaatatt tagaaatatt gccgtataag ctaggtgata atgaaccgat atttagagga aagcagggta agaaattgca gccaccggta tttaatcgtg aattaattaa attaaagcat ttttatggtt taccgcagca tttaactgct cattcattta ggcatagttt tgcttcgcat ttgcttgagc atggtgcaga tttacgctct attcaagagc tattaggtca taaaagtcta tctactacac aaaactatac taaaacaagt ataaagcatt tagaggctgt atatactacc gcatatccaa ttaaaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat