RifeA.00145.a

Aspartyl-tRNA synthetase

CENTER ID: RifeA.00145.a
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00145.a.B1.GE35854 Full length( RifeA.00145.a ) 1 600
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00145.a.B1.PS01661 Full length( RifeA.00145.a ) 1 600

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.1374
RefSeq: YP_247154.1
UniProt: Q4UKE2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHKYRTHNCN ELQISDVRKE VKLSGWVHRR RDHGNLVFID LRDHYGITQI VFTDQNQQLM EDASRLRYES VITVRGTIVA RSNDTINDTL PTGHIEVLAG EFIVESAADT LPFVINTEKD APEETRLKHR FLDLRREKLH NNIILRSQII SHIRHLMTGR GFTEFQTPIL TASSPEGARD FLVPSRMHPG KFYALPQAPQ QFKQLLMVSG FDRYFQIAPC FRDEDARADR SPGEFYQLDV EMSFVTQEDI FSTIEPVMYD LFTKFTAKKV SETPFVRIPY NESMLKYGSD KPDLRNPIII ADVTEIFRDS DFTIFRENIK KGSIVRAIPA PKAAALPRSF FDKMIEFAIS EGAGGLGYIQ FSETGEAKGP VAKFLSPQQL ESLKATASIS NGDAVFFASD KKEKAAKLAG KVRIRLGEEL DLLEKDCFKF CWITDFPFYE LNEDTGKIDF SHNPFSMPQG GLEALEQAKT TEELLELTAY QYDIVCNGIE LSSGAIRNHK PEIMYKAFSM AGYSEEEVDK RFGGMIRAFK FGAPPHGGIA PGIDRIVMLL AEATNIREII AFPLNQQAED LLMNAPSYVE DKALKELSIM LSPSILKNMK
NT Sequence
ATGCATAAAT ATAGAACTCA TAATTGTAAC GAATTACAAA TATCCGATGT TAGGAAAGAA GTTAAATTAT CCGGTTGGGT ACATAGAAGA AGAGATCACG GTAATCTAGT TTTCATAGAT TTACGCGATC ATTATGGTAT AACTCAAATC GTGTTTACCG ATCAAAACCA GCAGCTTATG GAAGATGCAA GTCGTTTGCG TTATGAGTCG GTAATTACGG TAAGGGGTAC AATTGTAGCA AGATCAAATG ATACTATTAA TGATACGCTT CCGACAGGTC ATATTGAGGT CTTAGCAGGT GAATTTATTG TTGAATCAGC TGCAGATACT CTACCTTTTG TTATTAATAC CGAAAAAGAT GCTCCAGAAG AGACGAGGCT TAAACATCGC TTTTTAGATC TTAGACGTGA GAAATTGCAT AATAATATAA TACTCCGCTC ACAAATTATT TCTCATATTC GTCATTTGAT GACGGGAAGA GGTTTTACCG AATTTCAAAC ACCGATTTTA ACGGCAAGTT CACCTGAAGG TGCTAGAGAT TTCTTAGTAC CGAGTAGAAT GCATCCGGGT AAGTTCTATG CGTTACCGCA AGCACCTCAG CAATTCAAGC AGTTATTGAT GGTATCAGGC TTTGATCGTT ATTTCCAAAT TGCTCCTTGT TTTCGGGATG AAGATGCAAG AGCCGATAGA TCACCTGGCG AGTTTTATCA GCTAGATGTA GAAATGTCGT TTGTTACACA GGAAGACATA TTTAGTACTA TTGAGCCTGT GATGTATGAT TTATTCACTA AATTCACGGC TAAAAAAGTA TCAGAGACTC CTTTTGTTCG TATTCCATAC AATGAATCTA TGCTAAAATA CGGTTCTGAT AAGCCTGATT TACGAAATCC TATTATAATT GCTGATGTAA CTGAGATATT TAGAGATTCC GATTTTACGA TTTTCAGAGA GAATATCAAA AAAGGTAGTA TTGTTCGTGC AATTCCTGCC CCTAAGGCAG CTGCACTGCC TCGTAGCTTC TTCGATAAAA TGATAGAGTT TGCAATATCC GAAGGAGCAG GTGGACTTGG CTATATTCAG TTTAGTGAAA CCGGTGAAGC AAAAGGACCG GTAGCAAAAT TCTTAAGCCC GCAGCAATTA GAGAGTCTAA AAGCTACAGC TAGTATTAGT AACGGTGATG CCGTATTTTT TGCTAGCGAT AAAAAAGAAA AAGCTGCAAA GCTTGCAGGC AAGGTTAGAA TAAGACTTGG TGAAGAGCTT GATTTACTCG AAAAAGACTG CTTTAAATTC TGCTGGATTA CCGATTTTCC ATTCTATGAA TTAAATGAAG ACACAGGAAA AATTGATTTT AGCCATAATC CATTTTCTAT GCCACAAGGT GGCTTAGAAG CTTTAGAGCA AGCTAAAACG ACTGAAGAGT TATTAGAGCT TACCGCATAT CAATATGATA TTGTTTGTAA CGGTATTGAG CTTTCTAGCG GTGCTATTAG AAACCATAAG CCTGAGATTA TGTATAAAGC ATTTTCTATG GCAGGTTATA GTGAAGAGGA AGTTGATAAG AGATTTGGGG GTATGATTAG AGCATTTAAG TTTGGTGCTC CACCACATGG CGGAATAGCT CCCGGTATTG ACAGAATCGT TATGCTACTC GCAGAAGCAA CTAATATAAG AGAAATAATT GCATTTCCTT TAAATCAGCA AGCTGAAGAT TTACTAATGA ACGCTCCAAG CTATGTAGAA GATAAAGCTC TAAAAGAGCT AAGCATTATG CTATCACCGT CGATTCTTAA GAATATGAAA
Details for RifeA.00145.a.B1.GE35854
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 46
Validated AA Sequence
MEDASRLRYE SVITVRGTIV ARSNDTINDT LPTGHIEVLA GEFIVESAAD TLPFVINTEK DAPEETRLKH RFLDLRREKL HNNIILRSQI ISHIRHLMTG RGFTEFQTPI LTASSPEGAR DFLVPSRMHP GKFYALPQAP QQFKQLLMVS GFDRYFQIAP CFRDEDARAD RSPGEFYQLD VEMSFXTQED IFSTIEPVMX XLXTKFTAKK VSXTPFVRIP YNESMLKYGX XKPDLRNPXI IXXVXXXXRX XXFTIFRXXX XKXVVLFVQX LPLRQXHCLV XXXIK
Validated NT Sequence
gccggatcct atcttactcn cttatttcat attcttaaga atcgacggtg atagcataat gcttagctct tttagagctt tatcttctac atagcttgga gcgttcatta gtaaatcttc agcttgctga tttaaaggaa atgcaattat ttctcttata ttagttgctt ctgcgagtag cataacgatt ctgtcaatac cgggagctat tccgccatgt ggtggagcac caaacttaaa tgctctaatc atacccccaa atctcttatc aacttcctct tcactataac ctgccataga aaatgcttta tacataatct caggcttatg gtttctaata gcaccgctag aaagctcaat accgttacaa acaatatcat attgatatgc ggtaagctct aataactctt cagtcgtttt agcttgctct aaagcttcta agccaccttg tggcatagaa aatggattat ggctaaaatc aatttttcct gtgtcttcat ttaattcata gaatggaaaa tcggtaatcc agcagaattt aaagcagtct ttttcgagta aatcaagctc ttcaccaagt cttattctaa ccttgcctgc aagctttgca gctttttctt ttttatcgct agcaaaaaat acggcatcac cgttactaat actagctgta ncttttagac tctctaattg ctgcgggctt annaattttg ctaccggtcc ttttgcttca ccggtttcac taaactgaat atanccaagt ccacctgctc cttnnnatat tgcaaactct atcattttat cnaanaanct acgaggcagt gcanctgcct taggggcagg anttgcacga acaanactac cntttttnna nnntnntctc tgaaaatcgt aaaatnnnnn tctctaannn tntcagntac ntcnncaatt atantaggat ttcgtaaatc aggcttntca gnnccgtatt ttagcataga ttcattgtat ggaatacgaa caaaaggagt ntctgatact tttttagccg tgaatttagt gantaantca tncatcacag gctcaatagt actaaatatg tcttcctgtg tancaaacga catttctaca tctagctgat aaaactcgcc aggtgatcta tcggctcttg catcttcatc ccgaaaacaa ggagcaattt ggaaataacg atcaaagcct gataccatca ataactgctt gaattgctga ggtgcttgcg gtaacgcata gaacttaccc ggatgcattc tactcggtac taagaaatct ctagcacctt caggtgaact tgccgttaaa atcggtgttt gaaattcggt aaaacctctt cccgtcatca aatgacgaat atgagaaata atttgtgagc ggagtattat attattatgc aatttctcac gtctaagatc taaaaagcga tgtttaagcc tcgtctcttc tggagcatct ttttcggtat taataacaaa aggtagagta tctgcagctg attcaacaat aaattcacct gctaagacct caatatgacc tgtcggaagc gtatcattaa tagtatcatt tgatcttgct acaattgtac cccttaccgt aattaccgac tcataacgca aacgacttgc atcttccata agctgctggt tttgatcggt aaacacgatt tgagttatac cataatgatc gcgtaaatct atgaaaacta gattaccgtg atctcttctt ctatgtaccc aaccggataa tttaacttct ttcctaacat cggatatttg taattcgtta caattatgag ttctata
Expected Protein Sequence
MAHHHHHHMH KYRTHNCNEL QISDVRKEVK LSGWVHRRRD HGNLVFIDLR DHYGITQIVF TDQNQQLMED ASRLRYESVI TVRGTIVARS NDTINDTLPT GHIEVLAGEF IVESAADTLP FVINTEKDAP EETRLKHRFL DLRREKLHNN IILRSQIISH IRHLMTGRGF TEFQTPILTA SSPEGARDFL VPSRMHPGKF YALPQAPQQF KQLLMVSGFD RYFQIAPCFR DEDARADRSP GEFYQLDVEM SFVTQEDIFS TIEPVMYDLF TKFTAKKVSE TPFVRIPYNE SMLKYGSDKP DLRNPIIIAD VTEIFRDSDF TIFRENIKKG SIVRAIPAPK AAALPRSFFD KMIEFAISEG AGGLGYIQFS ETGEAKGPVA KFLSPQQLES LKATASISNG DAVFFASDKK EKAAKLAGKV RIRLGEELDL LEKDCFKFCW ITDFPFYELN EDTGKIDFSH NPFSMPQGGL EALEQAKTTE ELLELTAYQY DIVCNGIELS SGAIRNHKPE IMYKAFSMAG YSEEEVDKRF GGMIRAFKFG APPHGGIAPG IDRIVMLLAE ATNIREIIAF PLNQQAEDLL MNAPSYVEDK ALKELSIMLS PSILKNMK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata aatatagaac tcataattgt aacgaattac aaatatccga tgttaggaaa gaagttaaat tatccggttg ggtacataga agaagagatc acggtaatct agttttcata gatttacgcg atcattatgg tataactcaa atcgtgttta ccgatcaaaa ccagcagctt atggaagatg caagtcgttt gcgttatgag tcggtaatta cggtaagggg tacaattgta gcaagatcaa atgatactat taatgatacg cttccgacag gtcatattga ggtcttagca ggtgaattta ttgttgaatc agctgcagat actctacctt ttgttattaa taccgaaaaa gatgctccag aagagacgag gcttaaacat cgctttttag atcttagacg tgagaaattg cataataata taatactccg ctcacaaatt atttctcata ttcgtcattt gatgacggga agaggtttta ccgaatttca aacaccgatt ttaacggcaa gttcacctga aggtgctaga gatttcttag taccgagtag aatgcatccg ggtaagttct atgcgttacc gcaagcacct cagcaattca agcagttatt gatggtatca ggctttgatc gttatttcca aattgctcct tgttttcggg atgaagatgc aagagccgat agatcacctg gcgagtttta tcagctagat gtagaaatgt cgtttgttac acaggaagac atatttagta ctattgagcc tgtgatgtat gatttattca ctaaattcac ggctaaaaaa gtatcagaga ctccttttgt tcgtattcca tacaatgaat ctatgctaaa atacggttct gataagcctg atttacgaaa tcctattata attgctgatg taactgagat atttagagat tccgatttta cgattttcag agagaatatc aaaaaaggta gtattgttcg tgcaattcct gcccctaagg cagctgcact gcctcgtagc ttcttcgata aaatgataga gtttgcaata tccgaaggag caggtggact tggctatatt cagtttagtg aaaccggtga agcaaaagga ccggtagcaa aattcttaag cccgcagcaa ttagagagtc taaaagctac agctagtatt agtaacggtg atgccgtatt ttttgctagc gataaaaaag aaaaagctgc aaagcttgca ggcaaggtta gaataagact tggtgaagag cttgatttac tcgaaaaaga ctgctttaaa ttctgctgga ttaccgattt tccattctat gaattaaatg aagacacagg aaaaattgat tttagccata atccattttc tatgccacaa ggtggcttag aagctttaga gcaagctaaa acgactgaag agttattaga gcttaccgca tatcaatatg atattgtttg taacggtatt gagctttcta gcggtgctat tagaaaccat aagcctgaga ttatgtataa agcattttct atggcaggtt atagtgaaga ggaagttgat aagagatttg ggggtatgat tagagcattt aagtttggtg ctccaccaca tggcggaata gctcccggta ttgacagaat cgttatgcta ctcgcagaag caactaatat aagagaaata attgcatttc ctttaaatca gcaagctgaa gatttactaa tgaacgctcc aagctatgta gaagataaag ctctaaaaga gctaagcatt atgctatcac cgtcgattct taagaatatg aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for RifeA.00145.a.B1.PS01661
PURIFICATION DATe: 10/24/2012
CONCENTRATION: 17.8mg/ml
OBSERVED MW: 73kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 86
PERCENT COVERAGE: 46
Protocol Notes
notes unavailable
Validated AA Sequence
MEDASRLRYE SVITVRGTIV ARSNDTINDT LPTGHIEVLA GEFIVESAAD TLPFVINTEK DAPEETRLKH RFLDLRREKL HNNIILRSQI ISHIRHLMTG RGFTEFQTPI LTASSPEGAR DFLVPSRMHP GKFYALPQAP QQFKQLLMVS GFDRYFQIAP CFRDEDARAD RSPGEFYQLD VEMSFXTQED IFSTIEPVMX XLXTKFTAKK VSXTPFVRIP YNESMLKYGX XKPDLRNPXI IXXVXXXXRX XXFTIFRXXX XKXVVLFVQX LPLRQXHCLV XXXIK
Validated NT Sequence
gccggatcct atcttactcn cttatttcat attcttaaga atcgacggtg atagcataat gcttagctct tttagagctt tatcttctac atagcttgga gcgttcatta gtaaatcttc agcttgctga tttaaaggaa atgcaattat ttctcttata ttagttgctt ctgcgagtag cataacgatt ctgtcaatac cgggagctat tccgccatgt ggtggagcac caaacttaaa tgctctaatc atacccccaa atctcttatc aacttcctct tcactataac ctgccataga aaatgcttta tacataatct caggcttatg gtttctaata gcaccgctag aaagctcaat accgttacaa acaatatcat attgatatgc ggtaagctct aataactctt cagtcgtttt agcttgctct aaagcttcta agccaccttg tggcatagaa aatggattat ggctaaaatc aatttttcct gtgtcttcat ttaattcata gaatggaaaa tcggtaatcc agcagaattt aaagcagtct ttttcgagta aatcaagctc ttcaccaagt cttattctaa ccttgcctgc aagctttgca gctttttctt ttttatcgct agcaaaaaat acggcatcac cgttactaat actagctgta ncttttagac tctctaattg ctgcgggctt annaattttg ctaccggtcc ttttgcttca ccggtttcac taaactgaat atanccaagt ccacctgctc cttnnnatat tgcaaactct atcattttat cnaanaanct acgaggcagt gcanctgcct taggggcagg anttgcacga acaanactac cntttttnna nnntnntctc tgaaaatcgt aaaatnnnnn tctctaannn tntcagntac ntcnncaatt atantaggat ttcgtaaatc aggcttntca gnnccgtatt ttagcataga ttcattgtat ggaatacgaa caaaaggagt ntctgatact tttttagccg tgaatttagt gantaantca tncatcacag gctcaatagt actaaatatg tcttcctgtg tancaaacga catttctaca tctagctgat aaaactcgcc aggtgatcta tcggctcttg catcttcatc ccgaaaacaa ggagcaattt ggaaataacg atcaaagcct gataccatca ataactgctt gaattgctga ggtgcttgcg gtaacgcata gaacttaccc ggatgcattc tactcggtac taagaaatct ctagcacctt caggtgaact tgccgttaaa atcggtgttt gaaattcggt aaaacctctt cccgtcatca aatgacgaat atgagaaata atttgtgagc ggagtattat attattatgc aatttctcac gtctaagatc taaaaagcga tgtttaagcc tcgtctcttc tggagcatct ttttcggtat taataacaaa aggtagagta tctgcagctg attcaacaat aaattcacct gctaagacct caatatgacc tgtcggaagc gtatcattaa tagtatcatt tgatcttgct acaattgtac cccttaccgt aattaccgac tcataacgca aacgacttgc atcttccata agctgctggt tttgatcggt aaacacgatt tgagttatac cataatgatc gcgtaaatct atgaaaacta gattaccgtg atctcttctt ctatgtaccc aaccggataa tttaacttct ttcctaacat cggatatttg taattcgtta caattatgag ttctata
Expressed Protein Sequence
MAHHHHHHMH KYRTHNCNEL QISDVRKEVK LSGWVHRRRD HGNLVFIDLR DHYGITQIVF TDQNQQLMED ASRLRYESVI TVRGTIVARS NDTINDTLPT GHIEVLAGEF IVESAADTLP FVINTEKDAP EETRLKHRFL DLRREKLHNN IILRSQIISH IRHLMTGRGF TEFQTPILTA SSPEGARDFL VPSRMHPGKF YALPQAPQQF KQLLMVSGFD RYFQIAPCFR DEDARADRSP GEFYQLDVEM SFVTQEDIFS TIEPVMYDLF TKFTAKKVSE TPFVRIPYNE SMLKYGSDKP DLRNPIIIAD VTEIFRDSDF TIFRENIKKG SIVRAIPAPK AAALPRSFFD KMIEFAISEG AGGLGYIQFS ETGEAKGPVA KFLSPQQLES LKATASISNG DAVFFASDKK EKAAKLAGKV RIRLGEELDL LEKDCFKFCW ITDFPFYELN EDTGKIDFSH NPFSMPQGGL EALEQAKTTE ELLELTAYQY DIVCNGIELS SGAIRNHKPE IMYKAFSMAG YSEEEVDKRF GGMIRAFKFG APPHGGIAPG IDRIVMLLAE ATNIREIIAF PLNQQAEDLL MNAPSYVEDK ALKELSIMLS PSILKNMK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCATA AATATAGAAC TCATAATTGT AACGAATTAC AAATATCCGA TGTTAGGAAA GAAGTTAAAT TATCCGGTTG GGTACATAGA AGAAGAGATC ACGGTAATCT AGTTTTCATA GATTTACGCG ATCATTATGG TATAACTCAA ATCGTGTTTA CCGATCAAAA CCAGCAGCTT ATGGAAGATG CAAGTCGTTT GCGTTATGAG TCGGTAATTA CGGTAAGGGG TACAATTGTA GCAAGATCAA ATGATACTAT TAATGATACG CTTCCGACAG GTCATATTGA GGTCTTAGCA GGTGAATTTA TTGTTGAATC AGCTGCAGAT ACTCTACCTT TTGTTATTAA TACCGAAAAA GATGCTCCAG AAGAGACGAG GCTTAAACAT CGCTTTTTAG ATCTTAGACG TGAGAAATTG CATAATAATA TAATACTCCG CTCACAAATT ATTTCTCATA TTCGTCATTT GATGACGGGA AGAGGTTTTA CCGAATTTCA AACACCGATT TTAACGGCAA GTTCACCTGA AGGTGCTAGA GATTTCTTAG TACCGAGTAG AATGCATCCG GGTAAGTTCT ATGCGTTACC GCAAGCACCT CAGCAATTCA AGCAGTTATT GATGGTATCA GGCTTTGATC GTTATTTCCA AATTGCTCCT TGTTTTCGGG ATGAAGATGC AAGAGCCGAT AGATCACCTG GCGAGTTTTA TCAGCTAGAT GTAGAAATGT CGTTTGTTAC ACAGGAAGAC ATATTTAGTA CTATTGAGCC TGTGATGTAT GATTTATTCA CTAAATTCAC GGCTAAAAAA GTATCAGAGA CTCCTTTTGT TCGTATTCCA TACAATGAAT CTATGCTAAA ATACGGTTCT GATAAGCCTG ATTTACGAAA TCCTATTATA ATTGCTGATG TAACTGAGAT ATTTAGAGAT TCCGATTTTA CGATTTTCAG AGAGAATATC AAAAAAGGTA GTATTGTTCG TGCAATTCCT GCCCCTAAGG CAGCTGCACT GCCTCGTAGC TTCTTCGATA AAATGATAGA GTTTGCAATA TCCGAAGGAG CAGGTGGACT TGGCTATATT CAGTTTAGTG AAACCGGTGA AGCAAAAGGA CCGGTAGCAA AATTCTTAAG CCCGCAGCAA TTAGAGAGTC TAAAAGCTAC AGCTAGTATT AGTAACGGTG ATGCCGTATT TTTTGCTAGC GATAAAAAAG AAAAAGCTGC AAAGCTTGCA GGCAAGGTTA GAATAAGACT TGGTGAAGAG CTTGATTTAC TCGAAAAAGA CTGCTTTAAA TTCTGCTGGA TTACCGATTT TCCATTCTAT GAATTAAATG AAGACACAGG AAAAATTGAT TTTAGCCATA ATCCATTTTC TATGCCACAA GGTGGCTTAG AAGCTTTAGA GCAAGCTAAA ACGACTGAAG AGTTATTAGA GCTTACCGCA TATCAATATG ATATTGTTTG TAACGGTATT GAGCTTTCTA GCGGTGCTAT TAGAAACCAT AAGCCTGAGA TTATGTATAA AGCATTTTCT ATGGCAGGTT ATAGTGAAGA GGAAGTTGAT AAGAGATTTG GGGGTATGAT TAGAGCATTT AAGTTTGGTG CTCCACCACA TGGCGGAATA GCTCCCGGTA TTGACAGAAT CGTTATGCTA CTCGCAGAAG CAACTAATAT AAGAGAAATA ATTGCATTTC CTTTAAATCA GCAAGCTGAA GATTTACTAA TGAACGCTCC AAGCTATGTA GAAGATAAAG CTCTAAAAGA GCTAAGCATT ATGCTATCAC CGTCGATTCT TAAGAATATG AAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT