RifeA.00137.a

UDP-N-acetylmuramate--L-alanine ligase

CENTER ID: RifeA.00137.a
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00137.a.B1.GE35853 Full length( RifeA.00137.a ) 1 478
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.1241
RefSeq: YP_247053.1
UniProt: Q4UKN9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLLEFKKTN QTLETIHFIG IGGVGMSGIA EILHNLGYKV QGSDLVENYN TKRLESYGIK IFLGHAEQNI TNVSYVVISS AINSNNPEIK EALERKIPII RRAEMLAELM RLKCSVAVSG SHGKTTTTSL VACLFEAAGL CPTVINGGII NNKSTNAYLG SSNYLIAEAD ESDATFIHIP STIAIITNID PEHLDYYKDF ETLIGAFKSF ITNLPFYGFA VCCIDHKIVR KLVDDITERK IVTYGIDSED AHIIAFNINT DIASSTFDVK ISLPNVLGTT IIEKITIPTP GRHNILNSLA AIAVGIELDF GIKAIKNGFN NFKGVKRRFT KVAEYNKAVV IDDYAHHPEE IKATLATAKN IADKQNGKVI AIFQPHRYSR MQHLFDDFML CFNDADMIYI TDIYAAGEEP IEGITSQNLV NKITKNKHHD QANFLAELDD AVEVIIDNAS SGDMIIMMGA GNISSFANEL DGRLSSRT
NT Sequence
ATGCTGTTAC TTGAATTTAA AAAAACTAAT CAAACTTTAG AAACTATACA TTTTATAGGT ATCGGCGGAG TTGGAATGAG CGGTATTGCC GAGATATTAC ATAATCTAGG TTATAAAGTA CAAGGTTCCG ATTTAGTAGA AAATTATAAT ACTAAAAGAC TTGAGTCATA CGGCATTAAA ATATTTTTAG GACATGCTGA GCAGAACATT ACCAATGTTT CGTATGTTGT TATTTCATCG GCAATTAATT CAAATAATCC TGAAATAAAA GAGGCATTAG AGCGTAAAAT TCCTATCATT AGACGTGCGG AAATGCTCGC TGAGCTTATG AGGTTAAAAT GTTCGGTAGC GGTTTCAGGA TCGCACGGTA AAACTACTAC TACCTCATTA GTTGCTTGTT TATTTGAGGC AGCCGGTTTA TGCCCTACGG TTATTAACGG TGGGATCATA AATAATAAAT CGACTAATGC ATATCTTGGT TCAAGTAATT ATTTAATTGC GGAGGCTGAT GAATCCGATG CGACGTTTAT TCATATTCCA TCAACTATTG CGATAATAAC TAATATTGAT CCTGAACATT TAGATTATTA TAAAGATTTT GAAACATTAA TAGGTGCGTT TAAAAGCTTT ATTACTAATT TGCCGTTTTA CGGTTTTGCT GTTTGTTGTA TCGATCATAA AATAGTTCGT AAGTTAGTAG ATGATATTAC TGAAAGGAAG ATTGTTACTT ATGGAATCGA TTCGGAAGAT GCTCATATTA TAGCGTTTAA TATTAATACT GATATTGCCT CCTCTACTTT TGACGTAAAA ATTAGTCTAC CAAATGTACT AGGTACAACG ATTATTGAAA AAATTACTAT TCCAACGCCC GGAAGACATA ACATTTTAAA TAGTCTTGCT GCAATTGCCG TTGGGATAGA ATTAGATTTC GGTATTAAAG CTATTAAAAA CGGTTTCAAT AATTTTAAAG GAGTGAAGAG GAGATTTACT AAAGTAGCGG AATATAATAA GGCGGTGGTT ATCGATGATT ACGCTCATCA TCCTGAGGAA ATCAAAGCAA CACTTGCGAC GGCTAAAAAT ATAGCCGATA AGCAAAATGG TAAAGTTATT GCTATTTTTC AGCCTCACAG ATATTCAAGA ATGCAGCATT TATTTGATGA TTTTATGCTT TGCTTTAATG ATGCTGATAT GATTTATATT ACAGATATAT ATGCTGCAGG AGAAGAGCCT ATAGAAGGGA TTACAAGTCA AAATTTAGTT AATAAGATTA CTAAAAATAA GCATCATGAC CAAGCAAATT TTTTAGCTGA ATTAGATGAT GCGGTTGAAG TTATTATAGA TAACGCATCT TCCGGCGATA TGATAATTAT GATGGGAGCA GGTAATATTT CAAGTTTTGC TAATGAATTA GATGGGAGAT TATCATCCCG CACT
Details for RifeA.00137.a.B1.GE35853
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 79
Validated AA Sequence
MAHHHHHHML LLEFKKTNQT LETIHFIGIG GVGMSGIAEI LHNLGYKVQG SDLVENYNTK RLESYGIKIF LGHAEQNITN VSYVVISSAI NSNNPEIKEA LERKIPIIRR AEMLAELMRL KCSVAVSGSH GKTTTTSLVA CLFEAAGLCP TVINGGIINN KSTNAYLGSS NYLIAEADES DATFIHIPST IAIITNIDPE HLDYYKDFET LIGAFKSFIT NLPFYGFAVC CIDHKIVRKL VDDITERKIV TYGIDSEDAH IIAFNINTDI ASSTFDVKIS LPNVLXTTII EKITIPTPGR XNILNSLAAX AVGIELDFXI XAXXNGFXXF XXXXXIYXXX XXXXXSMXTL XXXXXXXXXX LXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgctg ttacttgaat ttaaaaaaac taatcaaact ttagaaacta tacattttat aggtatcggc ggagttggaa tgagcggtat tgccgagata ttacataatc taggttataa agtacaaggt tccgatttag tagaaaatta taatactaaa agacttgagt catacggcat taaaatattt ttaggacatg ctgagcagaa cattaccaat gtttcgtatg ttgttatttc atcggcaatt aattcaaata atcctgaaat aaaagaggca ttagagcgta aaattcctat cattagacgt gcggaaatgc tcgctgagct tatgaggtta aaatgttcgg tagcggtttc aggatcgcac ggtaaaacta ctactacctc attagttgct tgtttatttg aggcagccgg tttatgccct acggttatta acggtgggat cataaataat aaatcgacta atgcatatct tggttcaagt aattatttaa ttgcggaggc tgatgaatcc gatgcgacgt ttattcatat tccatcaact attgcgataa taactaatat tgatcctgaa catttagatt attataaaga ttttgaaaca ttaataggtg cgtttaaaag ctttattact aatttgccgt tttacggttt tgctgtttgt tgtatcgatc ataaaatagt tcgtaagtta gtagatgata ttactgaaag gaagattgtt acttatggaa tcgattcgga agatgctcat attatagcgt ttaatattaa tactgatatt gcctcctcta cttttgacgt aaaaattagt ctaccaaatg tactangtac aacgattatt gaaaaaatta ctattccnac gcccggnaga nataacattt taaatagtct tgctgcnatn gccgtnggga tagaattaga tttcngtatt naagctnntn aaaacggttt cantnatttt nangnantna naganattta ctanntagnn nnnnannnnn gnnnntcgat gantacnctc atnncnnngn annnaannan nntgngnnnn ctannnnnnn nnnnnannna annnnnnnnt nnnnntnntn nnnnnncnna nnnnngnnnn nnnnnnnnnn nngnc
Expected Protein Sequence
MAHHHHHHML LLEFKKTNQT LETIHFIGIG GVGMSGIAEI LHNLGYKVQG SDLVENYNTK RLESYGIKIF LGHAEQNITN VSYVVISSAI NSNNPEIKEA LERKIPIIRR AEMLAELMRL KCSVAVSGSH GKTTTTSLVA CLFEAAGLCP TVINGGIINN KSTNAYLGSS NYLIAEADES DATFIHIPST IAIITNIDPE HLDYYKDFET LIGAFKSFIT NLPFYGFAVC CIDHKIVRKL VDDITERKIV TYGIDSEDAH IIAFNINTDI ASSTFDVKIS LPNVLGTTII EKITIPTPGR HNILNSLAAI AVGIELDFGI KAIKNGFNNF KGVKRRFTKV AEYNKAVVID DYAHHPEEIK ATLATAKNIA DKQNGKVIAI FQPHRYSRMQ HLFDDFMLCF NDADMIYITD IYAAGEEPIE GITSQNLVNK ITKNKHHDQA NFLAELDDAV EVIIDNASSG DMIIMMGAGN ISSFANELDG RLSSRT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgt tacttgaatt taaaaaaact aatcaaactt tagaaactat acattttata ggtatcggcg gagttggaat gagcggtatt gccgagatat tacataatct aggttataaa gtacaaggtt ccgatttagt agaaaattat aatactaaaa gacttgagtc atacggcatt aaaatatttt taggacatgc tgagcagaac attaccaatg tttcgtatgt tgttatttca tcggcaatta attcaaataa tcctgaaata aaagaggcat tagagcgtaa aattcctatc attagacgtg cggaaatgct cgctgagctt atgaggttaa aatgttcggt agcggtttca ggatcgcacg gtaaaactac tactacctca ttagttgctt gtttatttga ggcagccggt ttatgcccta cggttattaa cggtgggatc ataaataata aatcgactaa tgcatatctt ggttcaagta attatttaat tgcggaggct gatgaatccg atgcgacgtt tattcatatt ccatcaacta ttgcgataat aactaatatt gatcctgaac atttagatta ttataaagat tttgaaacat taataggtgc gtttaaaagc tttattacta atttgccgtt ttacggtttt gctgtttgtt gtatcgatca taaaatagtt cgtaagttag tagatgatat tactgaaagg aagattgtta cttatggaat cgattcggaa gatgctcata ttatagcgtt taatattaat actgatattg cctcctctac ttttgacgta aaaattagtc taccaaatgt actaggtaca acgattattg aaaaaattac tattccaacg cccggaagac ataacatttt aaatagtctt gctgcaattg ccgttgggat agaattagat ttcggtatta aagctattaa aaacggtttc aataatttta aaggagtgaa gaggagattt actaaagtag cggaatataa taaggcggtg gttatcgatg attacgctca tcatcctgag gaaatcaaag caacacttgc gacggctaaa aatatagccg ataagcaaaa tggtaaagtt attgctattt ttcagcctca cagatattca agaatgcagc atttatttga tgattttatg ctttgcttta atgatgctga tatgatttat attacagata tatatgctgc aggagaagag cctatagaag ggattacaag tcaaaattta gttaataaga ttactaaaaa taagcatcat gaccaagcaa attttttagc tgaattagat gatgcggttg aagttattat agataacgca tcttccggcg atatgataat tatgatggga gcaggtaata tttcaagttt tgctaatgaa ttagatggga gattatcatc ccgcacttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at