RifeA.00133.a

Cysteinyl-tRNA synthetase

CENTER ID: RifeA.00133.a
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00133.a.B1.GE35852 Full length( RifeA.00133.a ) 1 459
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00133.a.B1.PS01659 Full length( RifeA.00133.a ) 1 459

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.79
RefSeq: YP_246084.1
UniProt: Q4UND9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQIQFHLYNT LSRTKEVFNP QDQTKVKMYV CGPTVYDNPH IGNSRSVVVY DLLYRIVIKI FGEQSVKYVR NITDVDDKII DRAELLSITI NELTDKVTQE FHTNMAYLGC LLPSIEPKAT EHIDVMIEII ERLIAKDHAY IADNHVYFDV LSAPNYTELS NRNLEEMFEG VRIENSKTKK HPQDFVLWKP AKPNESANMN FKSPWGFGRP GWHIECSAMS YKYLGENFDI HGGGADLIFP HHTNEIAQSR CAFPDSTYAK YWVHNGFLTV NGEKMSKSLG NFITVRNLMD KEISGEVVRL FLLSSHYRRP LDYNDKAIED AKKTLDYWYR AIENINVQKI DLPHNFMQSL LDDMNTPLAV KIINDYAKGV FISKTEEEKQ LNASAIITCA NFIGLMNKTP HEWFNSGVDE LYINELLNKR LEAKKQKNWL LADQIRNQLL EEKIILEDKP DGTTIWRKE
NT Sequence
ATGCAAATTC AATTTCACTT ATATAACACT CTTAGCCGTA CTAAAGAGGT TTTTAACCCT CAAGATCAGA CTAAAGTAAA AATGTATGTA TGCGGACCGA CTGTTTACGA TAATCCTCAT ATAGGTAATA GCAGGTCGGT AGTGGTATAT GATTTACTCT ACCGTATAGT TATAAAAATA TTTGGTGAGC AATCTGTAAA ATACGTACGT AATATTACGG ATGTTGACGA TAAAATTATC GATAGGGCTG AGCTACTTAG CATTACTATT AATGAGTTGA CTGATAAAGT TACACAAGAG TTTCATACAA ATATGGCATA TTTAGGTTGC CTACTACCGA GTATTGAGCC GAAAGCTACA GAGCATATTG ATGTAATGAT CGAAATAATA GAACGCTTAA TAGCAAAAGA TCACGCATAT ATTGCGGATA ATCATGTTTA TTTTGATGTT TTATCAGCTC CAAATTATAC GGAATTATCT AACCGAAATT TAGAAGAAAT GTTTGAGGGA GTGCGTATAG AAAACAGCAA AACCAAAAAG CATCCACAGG ATTTTGTATT ATGGAAACCG GCAAAGCCAA ATGAATCCGC AAATATGAAT TTTAAAAGTC CTTGGGGATT TGGTCGCCCT GGATGGCATA TTGAATGTTC AGCCATGAGC TACAAATATT TAGGTGAGAA TTTTGATATT CACGGTGGCG GAGCAGATTT AATCTTCCCG CATCATACTA ATGAAATTGC ACAAAGTAGA TGTGCTTTTC CGGATTCTAC CTATGCTAAA TATTGGGTAC ATAATGGCTT TCTAACGGTG AACGGTGAGA AAATGAGTAA ATCTCTCGGT AATTTTATTA CCGTAAGGAA TTTAATGGAT AAAGAAATTT CAGGAGAGGT AGTAAGATTA TTCTTATTAA GTAGTCACTA TAGGCGTCCA CTTGATTATA ATGATAAAGC TATAGAAGAT GCTAAAAAGA CTTTAGATTA TTGGTATAGA GCCATAGAAA ATATTAATGT ACAAAAAATA GATTTACCTC ACAATTTCAT GCAAAGCTTA CTTGATGACA TGAATACGCC GCTTGCCGTT AAAATAATTA ATGACTATGC TAAAGGTGTT TTTATTTCTA AAACTGAAGA AGAAAAGCAG CTTAACGCCT CTGCTATTAT TACTTGTGCT AATTTTATTG GTTTAATGAA TAAGACTCCA CACGAGTGGT TTAATAGCGG TGTAGATGAA CTCTATATAA ACGAGCTTCT AAATAAGCGT TTGGAAGCAA AAAAACAAAA AAATTGGTTA TTAGCCGATC AAATTCGTAA TCAGTTATTA GAGGAAAAAA TAATTCTAGA AGATAAACCT GATGGTACTA CTATTTGGCG AAAAGAA
Details for RifeA.00133.a.B1.GE35852
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 54kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 90
Validated AA Sequence
MYVCGPTVYD NPHIGNSRSV VVYDLLYRIV IKIFGEQSVK YVRNITDVDD KIIDRAELLS ITINELTDKV TQEFHTNMAY LGCLLPSIEP KATEHIDVMI EIIERLIAKD HAYIADNHVY FDVLSAPNYT ELSNRNLEEM FEGVRIENSK TKKHPQDFVL WKPAKPNESA NMNFKSPWGF GRPGWHIECS AMSYKYLGEN FDIHGGGADL IFPHHTNEIA QSRCAFPDST YAKYWVHNGF LTVNGEKMSK SLGNFITVRN LMDKEISGEV VRLFLLSSHY RRPLDYNDKA IEDAKKTLDY WYRAIENINV QKIDLPHNFM QSLLDDMNTP LAVKIINDYA KGVFISKTEE EKQLNASAII TCANFIGLMN KTPHEWFNSG VDELYINELL NKRLEAKKQK NWLLADQIRN QLLEEKIILE DKPD
Validated NT Sequence
ccatcaggtt tatcttctag aattattttt tcctctaata actgattacg aatttgatcg gctaataacc aatttttttg tttttttgct tccaaacgct tatttagaag ctcgtttata tagagttcat ctacaccgct attaaaccac tcgtgtggag tcttattcat taaaccaata aaattagcac aagtaataat agcagaggcg ttaagctgct tttcttcttc agttttagaa ataaaaacac ctttagcata gtcattaatt attttaacgg caagcggcgt attcatgtca tcaagtaagc tttgcatgaa attgtgaggt aaatctattt tttgtacatt aatattttct atggctctat accaataatc taaagtcttt ttagcatctt ctatagcttt atcattataa tcaagtggac gcctatagtg actacttaat aagaataatc ttactacctc tcctgaaatt tctttatcca ttaaattcct tacggtaata aaattaccga gagatttact cattttctca ccgttcaccg ttagaaagcc attatgtacc caatatttag cataggtaga atccggaaaa gcacatctac tttgtgcaat ttcattagta tgatgcggga agattaaatc tgctccgcca ccgtgaatat caaaattctc acctaaatat ttgtagctca tggctgaaca ttcaatatgc catccagggc gaccaaatcc ccaaggactt ttaaaattca tatttgcgga ttcatttggc tttgccggtt tccataatac aaaatcctgt ggatgctttt tggttttgct gttttctata cgcactccct caaacatttc ttctaaattt cggttagata attccgtata atttggagct gataaaacat caaaataaac atgattatcc gcaatatatg cgtgatcttt tgctattaag cgttctatta tttcgatcat tacatcaata tgctctgtag ctttcggctc aatactcggt agtaggcaac ctaaatatgc catatttgta tgaaactctt gtgtaacttt atcagtcaac tcattaatag taatgctaag tagctcagcc ctatcgataa ttttatcgtc aacatccgta atattacgta cgtattttac agattgctca ccaaatattt ttataactat acggtagagt aaatcatata ccactaccga cctgctatta cctatatgag gattatcgta aacagtcggt ccgcatacat acatttttac tttagtctga tcttgagggt taaaaacctc tttagtacgg ctaagagtgt tatataagtg aaattg
Expected Protein Sequence
MAHHHHHHMQ IQFHLYNTLS RTKEVFNPQD QTKVKMYVCG PTVYDNPHIG NSRSVVVYDL LYRIVIKIFG EQSVKYVRNI TDVDDKIIDR AELLSITINE LTDKVTQEFH TNMAYLGCLL PSIEPKATEH IDVMIEIIER LIAKDHAYIA DNHVYFDVLS APNYTELSNR NLEEMFEGVR IENSKTKKHP QDFVLWKPAK PNESANMNFK SPWGFGRPGW HIECSAMSYK YLGENFDIHG GGADLIFPHH TNEIAQSRCA FPDSTYAKYW VHNGFLTVNG EKMSKSLGNF ITVRNLMDKE ISGEVVRLFL LSSHYRRPLD YNDKAIEDAK KTLDYWYRAI ENINVQKIDL PHNFMQSLLD DMNTPLAVKI INDYAKGVFI SKTEEEKQLN ASAIITCANF IGLMNKTPHE WFNSGVDELY INELLNKRLE AKKQKNWLLA DQIRNQLLEE KIILEDKPDG TTIWRKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa ttcaatttca cttatataac actcttagcc gtactaaaga ggtttttaac cctcaagatc agactaaagt aaaaatgtat gtatgcggac cgactgttta cgataatcct catataggta atagcaggtc ggtagtggta tatgatttac tctaccgtat agttataaaa atatttggtg agcaatctgt aaaatacgta cgtaatatta cggatgttga cgataaaatt atcgataggg ctgagctact tagcattact attaatgagt tgactgataa agttacacaa gagtttcata caaatatggc atatttaggt tgcctactac cgagtattga gccgaaagct acagagcata ttgatgtaat gatcgaaata atagaacgct taatagcaaa agatcacgca tatattgcgg ataatcatgt ttattttgat gttttatcag ctccaaatta tacggaatta tctaaccgaa atttagaaga aatgtttgag ggagtgcgta tagaaaacag caaaaccaaa aagcatccac aggattttgt attatggaaa ccggcaaagc caaatgaatc cgcaaatatg aattttaaaa gtccttgggg atttggtcgc cctggatggc atattgaatg ttcagccatg agctacaaat atttaggtga gaattttgat attcacggtg gcggagcaga tttaatcttc ccgcatcata ctaatgaaat tgcacaaagt agatgtgctt ttccggattc tacctatgct aaatattggg tacataatgg ctttctaacg gtgaacggtg agaaaatgag taaatctctc ggtaatttta ttaccgtaag gaatttaatg gataaagaaa tttcaggaga ggtagtaaga ttattcttat taagtagtca ctataggcgt ccacttgatt ataatgataa agctatagaa gatgctaaaa agactttaga ttattggtat agagccatag aaaatattaa tgtacaaaaa atagatttac ctcacaattt catgcaaagc ttacttgatg acatgaatac gccgcttgcc gttaaaataa ttaatgacta tgctaaaggt gtttttattt ctaaaactga agaagaaaag cagcttaacg cctctgctat tattacttgt gctaatttta ttggtttaat gaataagact ccacacgagt ggtttaatag cggtgtagat gaactctata taaacgagct tctaaataag cgtttggaag caaaaaaaca aaaaaattgg ttattagccg atcaaattcg taatcagtta ttagaggaaa aaataattct agaagataaa cctgatggta ctactatttg gcgaaaagaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for RifeA.00133.a.B1.PS01659
PURIFICATION DATe: 10/18/2012
CONCENTRATION: 55mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 90
Protocol Notes
notes unavailable
Validated AA Sequence
MYVCGPTVYD NPHIGNSRSV VVYDLLYRIV IKIFGEQSVK YVRNITDVDD KIIDRAELLS ITINELTDKV TQEFHTNMAY LGCLLPSIEP KATEHIDVMI EIIERLIAKD HAYIADNHVY FDVLSAPNYT ELSNRNLEEM FEGVRIENSK TKKHPQDFVL WKPAKPNESA NMNFKSPWGF GRPGWHIECS AMSYKYLGEN FDIHGGGADL IFPHHTNEIA QSRCAFPDST YAKYWVHNGF LTVNGEKMSK SLGNFITVRN LMDKEISGEV VRLFLLSSHY RRPLDYNDKA IEDAKKTLDY WYRAIENINV QKIDLPHNFM QSLLDDMNTP LAVKIINDYA KGVFISKTEE EKQLNASAII TCANFIGLMN KTPHEWFNSG VDELYINELL NKRLEAKKQK NWLLADQIRN QLLEEKIILE DKPD
Validated NT Sequence
ccatcaggtt tatcttctag aattattttt tcctctaata actgattacg aatttgatcg gctaataacc aatttttttg tttttttgct tccaaacgct tatttagaag ctcgtttata tagagttcat ctacaccgct attaaaccac tcgtgtggag tcttattcat taaaccaata aaattagcac aagtaataat agcagaggcg ttaagctgct tttcttcttc agttttagaa ataaaaacac ctttagcata gtcattaatt attttaacgg caagcggcgt attcatgtca tcaagtaagc tttgcatgaa attgtgaggt aaatctattt tttgtacatt aatattttct atggctctat accaataatc taaagtcttt ttagcatctt ctatagcttt atcattataa tcaagtggac gcctatagtg actacttaat aagaataatc ttactacctc tcctgaaatt tctttatcca ttaaattcct tacggtaata aaattaccga gagatttact cattttctca ccgttcaccg ttagaaagcc attatgtacc caatatttag cataggtaga atccggaaaa gcacatctac tttgtgcaat ttcattagta tgatgcggga agattaaatc tgctccgcca ccgtgaatat caaaattctc acctaaatat ttgtagctca tggctgaaca ttcaatatgc catccagggc gaccaaatcc ccaaggactt ttaaaattca tatttgcgga ttcatttggc tttgccggtt tccataatac aaaatcctgt ggatgctttt tggttttgct gttttctata cgcactccct caaacatttc ttctaaattt cggttagata attccgtata atttggagct gataaaacat caaaataaac atgattatcc gcaatatatg cgtgatcttt tgctattaag cgttctatta tttcgatcat tacatcaata tgctctgtag ctttcggctc aatactcggt agtaggcaac ctaaatatgc catatttgta tgaaactctt gtgtaacttt atcagtcaac tcattaatag taatgctaag tagctcagcc ctatcgataa ttttatcgtc aacatccgta atattacgta cgtattttac agattgctca ccaaatattt ttataactat acggtagagt aaatcatata ccactaccga cctgctatta cctatatgag gattatcgta aacagtcggt ccgcatacat acatttttac tttagtctga tcttgagggt taaaaacctc tttagtacgg ctaagagtgt tatataagtg aaattg
Expressed Protein Sequence
MAHHHHHHMQ IQFHLYNTLS RTKEVFNPQD QTKVKMYVCG PTVYDNPHIG NSRSVVVYDL LYRIVIKIFG EQSVKYVRNI TDVDDKIIDR AELLSITINE LTDKVTQEFH TNMAYLGCLL PSIEPKATEH IDVMIEIIER LIAKDHAYIA DNHVYFDVLS APNYTELSNR NLEEMFEGVR IENSKTKKHP QDFVLWKPAK PNESANMNFK SPWGFGRPGW HIECSAMSYK YLGENFDIHG GGADLIFPHH TNEIAQSRCA FPDSTYAKYW VHNGFLTVNG EKMSKSLGNF ITVRNLMDKE ISGEVVRLFL LSSHYRRPLD YNDKAIEDAK KTLDYWYRAI ENINVQKIDL PHNFMQSLLD DMNTPLAVKI INDYAKGVFI SKTEEEKQLN ASAIITCANF IGLMNKTPHE WFNSGVDELY INELLNKRLE AKKQKNWLLA DQIRNQLLEE KIILEDKPDG TTIWRKE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA TTCAATTTCA CTTATATAAC ACTCTTAGCC GTACTAAAGA GGTTTTTAAC CCTCAAGATC AGACTAAAGT AAAAATGTAT GTATGCGGAC CGACTGTTTA CGATAATCCT CATATAGGTA ATAGCAGGTC GGTAGTGGTA TATGATTTAC TCTACCGTAT AGTTATAAAA ATATTTGGTG AGCAATCTGT AAAATACGTA CGTAATATTA CGGATGTTGA CGATAAAATT ATCGATAGGG CTGAGCTACT TAGCATTACT ATTAATGAGT TGACTGATAA AGTTACACAA GAGTTTCATA CAAATATGGC ATATTTAGGT TGCCTACTAC CGAGTATTGA GCCGAAAGCT ACAGAGCATA TTGATGTAAT GATCGAAATA ATAGAACGCT TAATAGCAAA AGATCACGCA TATATTGCGG ATAATCATGT TTATTTTGAT GTTTTATCAG CTCCAAATTA TACGGAATTA TCTAACCGAA ATTTAGAAGA AATGTTTGAG GGAGTGCGTA TAGAAAACAG CAAAACCAAA AAGCATCCAC AGGATTTTGT ATTATGGAAA CCGGCAAAGC CAAATGAATC CGCAAATATG AATTTTAAAA GTCCTTGGGG ATTTGGTCGC CCTGGATGGC ATATTGAATG TTCAGCCATG AGCTACAAAT ATTTAGGTGA GAATTTTGAT ATTCACGGTG GCGGAGCAGA TTTAATCTTC CCGCATCATA CTAATGAAAT TGCACAAAGT AGATGTGCTT TTCCGGATTC TACCTATGCT AAATATTGGG TACATAATGG CTTTCTAACG GTGAACGGTG AGAAAATGAG TAAATCTCTC GGTAATTTTA TTACCGTAAG GAATTTAATG GATAAAGAAA TTTCAGGAGA GGTAGTAAGA TTATTCTTAT TAAGTAGTCA CTATAGGCGT CCACTTGATT ATAATGATAA AGCTATAGAA GATGCTAAAA AGACTTTAGA TTATTGGTAT AGAGCCATAG AAAATATTAA TGTACAAAAA ATAGATTTAC CTCACAATTT CATGCAAAGC TTACTTGATG ACATGAATAC GCCGCTTGCC GTTAAAATAA TTAATGACTA TGCTAAAGGT GTTTTTATTT CTAAAACTGA AGAAGAAAAG CAGCTTAACG CCTCTGCTAT TATTACTTGT GCTAATTTTA TTGGTTTAAT GAATAAGACT CCACACGAGT GGTTTAATAG CGGTGTAGAT GAACTCTATA TAAACGAGCT TCTAAATAAG CGTTTGGAAG CAAAAAAACA AAAAAATTGG TTATTAGCCG ATCAAATTCG TAATCAGTTA TTAGAGGAAA AAATAATTCT AGAAGATAAA CCTGATGGTA CTACTATTTG GCGAAAAGAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT