SSGCID: Target Page

RifeA.00081.a

Nifs protein homolog

CENTER ID:	RifeA.00081.a
ORGANISM:	Rickettsia felis URRWXCal2
ASSOCIATED DISEASE:	Flea-borne spotted fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.00081.a.B1.GE35849	Full length( RifeA.00081.a )		1	369

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RifeA.00081.a.B1.PS01654	Full length( RifeA.00081.a )		1	369

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|315456.7.peg.1014
RefSeq:	YP_246862.1
UniProt:	Q4UL76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIYLDHNATT FIDPKVKGYI ISLMDKELNP SSIHRSGVFA RNVIEEARFQ LAAALGISLF AGKYDITFTS SGTESNNLIM KNFYDGDIFI SAIEHLSIYN HINYAPNIKV IKVDNQGLLD LEHLEELLSQ SSATKKLVSI IMANNESGVL QDITKISEIA KKYDAKFHSD LVQGFGRIHI NIKELGLDFA TISGHKIGGG QGGAALISTS RFQLTPIIIG GGQEKSVRSG TENVLAIAGL GFAAELRRES ISENYIKIKD LQEILEKKLK KYPKVNIISR NAQRLPNTTL FTVPGTDAQV KLIGFDLRNI CVSSGSACSS GKISKSHVLT NMGMSEEEAK SSIRISLSHA NTIEDIEVFI KAFEEIYDI

NT Sequence

ATGATATATT TAGACCATAA TGCCACTACC TTTATCGATC CCAAGGTTAA AGGATATATA ATAAGTTTGA TGGATAAAGA GCTTAACCCT TCGTCAATAC ATAGATCAGG TGTGTTTGCT AGGAATGTTA TAGAAGAAGC CCGTTTTCAA TTAGCTGCAG CACTTGGTAT ATCATTATTT GCGGGAAAAT ATGATATTAC TTTTACTTCT TCCGGAACTG AAAGTAATAA TTTAATAATG AAAAATTTTT ATGACGGTGA TATTTTCATA TCGGCTATTG AACATTTATC GATCTATAAC CATATAAACT ACGCTCCAAA TATTAAAGTT ATAAAAGTTG ATAATCAAGG TTTGCTTGAT CTAGAGCATC TAGAAGAATT ATTGTCACAA AGTAGCGCTA CAAAAAAATT AGTATCTATA ATTATGGCTA ATAATGAAAG CGGGGTGCTG CAAGATATAA CCAAAATAAG TGAAATAGCT AAAAAATATG ATGCAAAATT TCATAGTGAT TTAGTGCAAG GTTTTGGCAG AATACATATA AATATCAAAG AGTTAGGATT AGATTTTGCT ACAATCTCAG GACATAAAAT AGGAGGTGGG CAGGGAGGAG CTGCTTTAAT TTCTACTTCT CGCTTTCAGC TTACTCCGAT AATTATAGGA GGTGGGCAGG AAAAATCCGT ACGCTCAGGT ACTGAAAATG TTTTAGCAAT TGCAGGACTT GGTTTTGCGG CTGAACTGAG AAGAGAAAGC ATCTCGGAAA ATTACATAAA AATCAAAGAC TTACAAGAAA TTTTAGAGAA AAAATTAAAA AAATATCCTA AAGTAAATAT TATTAGTAGA AATGCACAAA GATTACCTAA TACTACTTTA TTTACTGTAC CAGGTACGGA TGCACAAGTG AAATTAATCG GATTTGATTT ACGTAATATT TGTGTAAGTT CCGGCTCTGC TTGTTCATCA GGAAAAATAT CTAAATCGCA CGTGTTAACC AATATGGGCA TGAGTGAAGA AGAGGCAAAA TCTTCAATTA GGATATCTTT AAGCCATGCT AATACAATTG AGGATATAGA AGTATTTATA AAAGCTTTTG AGGAGATTTA TGATATA

Details for RifeA.00081.a.B1.GE35849

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	90

Validated AA Sequence

MDKELNPSSI HRSGVFARNV IEEARFQLAA ALGISLFAGK YDITFTSSGT ESNNLIMKNF YDGDIFISAI EHLSIYNHIN YAPNIKVIKV DNQGLLDLEH LEELLSQSSA TKKLVSIIMA NNESGVLQDI TKISEIAKKY DAKFHSDLVQ GFGRIHINIK ELGLDFATIS GHKIGGGQGG AALISTSRFQ LTPIIIGGGQ EKSVRSGTEN VLAIAGLGFA AELRRESISE NYIKIKDLQE ILEKKLKKYP KVNIISRNAQ RLPNTTLFTV PGTDAQVKLI GFDLRNICVS SGSACSSGKI SKSHVLTNMG MSEEEAKSSI RISLSHANTI EDIEVFIKAF EE

Validated NT Sequence

aatctcctca aaagctttta taaatacttc tatatcctca attgtattag catggcttaa agatatccta attgaagatt ttgcctcttc ttcactcatg cccatattgg ttaacacgtg cgatttagat atttttcctg atgaacaagc agagccggaa cttacacaaa tattacgtaa atcaaatccg attaatttca cttgtgcatc cgtacctggt acagtaaata aagtagtatt aggtaatctt tgtgcatttc tactaataat atttacttta ggatattttt ttaatttttt ctctaaaatt tcttgtaagt ctttgatttt tatgtaattt tccgagatgc tttctcttct cagttcagcc gcaaaaccaa gtcctgcaat tgctaaaaca ttttcagtac ctgagcgtac ggatttttcc tgcccacctc ctataattat cggagtaagc tgaaagcgag aagtagaaat taaagcagct cctccctgcc cacctcctat tttatgtcct gagattgtag caaaatctaa tcctaactct ttgatattta tatgtattct gccaaaacct tgcactaaat cactatgaaa ttttgcatca tattttttag ctatttcact tattttggtt atatcttgca gcaccccgct ttcattatta gccataatta tagatactaa tttttttgta gcgctacttt gtgacaataa ttcttctaga tgctctagat caagcaaacc ttgattatca acttttataa ctttaatatt tggagcgtag tttatatggt tatagatcga taaatgttca atagccgata tgaaaatatc accgtcataa aaatttttca ttattaaatt attactttca gttccggaag aagtaaaagt aatatcatat tttcccgcaa ataatgatat accaagtgct gcagctaatt gaaaacgggc ttcttctata acattcctag caaacacacc tgatctatgt attgacgaag ggttaagctc tttatccatc aaacttatta tatatccttt aaccttggga tcgataaagg tagtggcatt atggtctaaa t

Expected Protein Sequence

MAHHHHHHMI YLDHNATTFI DPKVKGYIIS LMDKELNPSS IHRSGVFARN VIEEARFQLA AALGISLFAG KYDITFTSSG TESNNLIMKN FYDGDIFISA IEHLSIYNHI NYAPNIKVIK VDNQGLLDLE HLEELLSQSS ATKKLVSIIM ANNESGVLQD ITKISEIAKK YDAKFHSDLV QGFGRIHINI KELGLDFATI SGHKIGGGQG GAALISTSRF QLTPIIIGGG QEKSVRSGTE NVLAIAGLGF AAELRRESIS ENYIKIKDLQ EILEKKLKKY PKVNIISRNA QRLPNTTLFT VPGTDAQVKL IGFDLRNICV SSGSACSSGK ISKSHVLTNM GMSEEEAKSS IRISLSHANT IEDIEVFIKA FEEIYDI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatat atttagacca taatgccact acctttatcg atcccaaggt taaaggatat ataataagtt tgatggataa agagcttaac ccttcgtcaa tacatagatc aggtgtgttt gctaggaatg ttatagaaga agcccgtttt caattagctg cagcacttgg tatatcatta tttgcgggaa aatatgatat tacttttact tcttccggaa ctgaaagtaa taatttaata atgaaaaatt tttatgacgg tgatattttc atatcggcta ttgaacattt atcgatctat aaccatataa actacgctcc aaatattaaa gttataaaag ttgataatca aggtttgctt gatctagagc atctagaaga attattgtca caaagtagcg ctacaaaaaa attagtatct ataattatgg ctaataatga aagcggggtg ctgcaagata taaccaaaat aagtgaaata gctaaaaaat atgatgcaaa atttcatagt gatttagtgc aaggttttgg cagaatacat ataaatatca aagagttagg attagatttt gctacaatct caggacataa aataggaggt gggcagggag gagctgcttt aatttctact tctcgctttc agcttactcc gataattata ggaggtgggc aggaaaaatc cgtacgctca ggtactgaaa atgttttagc aattgcagga cttggttttg cggctgaact gagaagagaa agcatctcgg aaaattacat aaaaatcaaa gacttacaag aaattttaga gaaaaaatta aaaaaatatc ctaaagtaaa tattattagt agaaatgcac aaagattacc taatactact ttatttactg taccaggtac ggatgcacaa gtgaaattaa tcggatttga tttacgtaat atttgtgtaa gttccggctc tgcttgttca tcaggaaaaa tatctaaatc gcacgtgtta accaatatgg gcatgagtga agaagaggca aaatcttcaa ttaggatatc tttaagccat gctaatacaa ttgaggatat agaagtattt ataaaagctt ttgaggagat ttatgatata tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for RifeA.00081.a.B1.PS01654

PURIFICATION DATe:	10/18/2012
CONCENTRATION:	49.2mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	90

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATAT ATTTAGACCA TAATGCCACT ACCTTTATCG ATCCCAAGGT TAAAGGATAT ATAATAAGTT TGATGGATAA AGAGCTTAAC CCTTCGTCAA TACATAGATC AGGTGTGTTT GCTAGGAATG TTATAGAAGA AGCCCGTTTT CAATTAGCTG CAGCACTTGG TATATCATTA TTTGCGGGAA AATATGATAT TACTTTTACT TCTTCCGGAA CTGAAAGTAA TAATTTAATA ATGAAAAATT TTTATGACGG TGATATTTTC ATATCGGCTA TTGAACATTT ATCGATCTAT AACCATATAA ACTACGCTCC AAATATTAAA GTTATAAAAG TTGATAATCA AGGTTTGCTT GATCTAGAGC ATCTAGAAGA ATTATTGTCA CAAAGTAGCG CTACAAAAAA ATTAGTATCT ATAATTATGG CTAATAATGA AAGCGGGGTG CTGCAAGATA TAACCAAAAT AAGTGAAATA GCTAAAAAAT ATGATGCAAA ATTTCATAGT GATTTAGTGC AAGGTTTTGG CAGAATACAT ATAAATATCA AAGAGTTAGG ATTAGATTTT GCTACAATCT CAGGACATAA AATAGGAGGT GGGCAGGGAG GAGCTGCTTT AATTTCTACT TCTCGCTTTC AGCTTACTCC GATAATTATA GGAGGTGGGC AGGAAAAATC CGTACGCTCA GGTACTGAAA ATGTTTTAGC AATTGCAGGA CTTGGTTTTG CGGCTGAACT GAGAAGAGAA AGCATCTCGG AAAATTACAT AAAAATCAAA GACTTACAAG AAATTTTAGA GAAAAAATTA AAAAAATATC CTAAAGTAAA TATTATTAGT AGAAATGCAC AAAGATTACC TAATACTACT TTATTTACTG TACCAGGTAC GGATGCACAA GTGAAATTAA TCGGATTTGA TTTACGTAAT ATTTGTGTAA GTTCCGGCTC TGCTTGTTCA TCAGGAAAAA TATCTAAATC GCACGTGTTA ACCAATATGG GCATGAGTGA AGAAGAGGCA AAATCTTCAA TTAGGATATC TTTAAGCCAT GCTAATACAA TTGAGGATAT AGAAGTATTT ATAAAAGCTT TTGAGGAGAT TTATGATATA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT