RifeA.00008.a

Serine hydroxymethyltransferase

CENTER ID: RifeA.00008.a
ORGANISM: Rickettsia felis URRWXCal2
ASSOCIATED DISEASE: Flea-borne spotted fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00008.a.B1.GE35900 Full length( RifeA.00008.a ) 1 421
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RifeA.00008.a.B1.PS01658 Full length( RifeA.00008.a ) 1 421

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|315456.7.peg.1429
RefSeq: YP_247204.1
UniProt: Q4UK96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIFNNHLHE TDKEIDEIIK HEKLRQNSVI ELIASENFVS PAVLEAQGSI LTNKYAEGYS GKRFYNGCEE VDKAENLAIE RVKKLFNCKY ANVQSHSGSQ ANQAVYLALL QPGDTILGMS LDSGGHLTHG AAPNMSGKWF NAVSYSVNKE TYLIDYDEIE RLADLHKPKL LIAGFSAYPR NIDFARFREI ADKVGAYFMA DIAHIAGLVA TGEHQSPIPY THAVTSTTHK TLRGPRGGLI LSNDEEIGKK INSALFPGLQ GGPLMHIIAA KAVAFLENLQ PEYKSYIKQV ISNAKALASS LQERGYDILT GGTDNHIVLV DLCKDGITGK LAANSLDRAG ITCNKNAIPF DETSPFITSG IRLGTPACTT RGFKEKDFVL VGHMVADILD GLKNNEDNGK AEQKVLTEVT KLIKLFPFYT R
NT Sequence
ATGAATATTT TTAATAATCA CTTACACGAA ACGGATAAAG AAATTGATGA AATAATCAAG CATGAAAAGC TGCGTCAAAA TAGCGTAATT GAGCTTATTG CATCAGAGAA TTTCGTAAGT CCCGCAGTGC TTGAAGCTCA AGGATCGATT CTGACTAATA AATATGCGGA AGGGTATTCA GGTAAGCGTT TCTATAACGG TTGTGAGGAA GTCGATAAAG CTGAGAATTT AGCTATAGAG CGAGTAAAAA AACTCTTTAA CTGTAAATAT GCAAATGTGC AGTCTCATTC CGGTTCACAA GCAAATCAAG CTGTGTATCT TGCTTTATTA CAGCCTGGTG ATACAATTCT TGGTATGTCT TTAGATAGCG GCGGGCATCT AACGCACGGT GCAGCCCCGA ATATGTCCGG TAAATGGTTT AACGCCGTTT CTTATAGCGT AAATAAAGAG ACTTATTTAA TCGATTATGA TGAGATTGAA CGCTTAGCGG ATTTGCATAA ACCAAAATTA CTTATAGCCG GTTTTTCTGC TTATCCTCGT AATATTGATT TTGCAAGATT TAGAGAAATA GCGGATAAAG TAGGAGCTTA CTTTATGGCA GATATTGCTC ATATTGCCGG ACTTGTTGCC ACAGGCGAGC ATCAAAGCCC TATTCCTTAC ACTCATGCTG TTACCTCTAC CACTCACAAA ACGCTCCGAG GACCGCGAGG CGGTTTAATT TTATCTAACG ACGAAGAGAT AGGCAAAAAG ATAAATTCTG CATTATTTCC AGGACTACAA GGCGGTCCGT TAATGCATAT AATCGCCGCA AAAGCCGTAG CTTTTTTAGA AAATTTGCAG CCTGAATATA AAAGCTATAT TAAGCAGGTA ATAAGTAATG CTAAAGCTTT AGCAAGTAGC TTGCAAGAAA GAGGATATGA TATATTAACA GGCGGTACCG ATAATCATAT TGTTTTAGTG GATTTGTGTA AAGACGGGAT TACCGGAAAG CTTGCTGCTA ATTCTTTGGA TAGAGCAGGG ATTACATGTA ATAAGAATGC TATCCCCTTT GATGAAACTT CGCCTTTTAT CACTTCAGGT ATTCGTCTTG GCACTCCGGC ATGCACTACT AGAGGATTTA AAGAAAAGGA TTTTGTATTA GTCGGTCATA TGGTAGCAGA TATTTTAGAC GGCTTAAAAA ATAATGAAGA TAATGGTAAA GCCGAGCAAA AAGTACTAAC TGAAGTAACA AAACTAATTA AATTATTTCC GTTTTATACT CGG
Details for RifeA.00008.a.B1.GE35900
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 66
Validated AA Sequence
MSLDSGGHLT HGAAPNMSGK WFNAVSYSVN KETYLIDYDE IERLADLHKP KLLIAGFSAY PRNIDFARFR EIADKVGAYF MADIAHIAGL VATGEHQSPI PYTHAVTSTT HKTLRGPRGG LILSNDEEIG KKINSALFPG LQGGPLMHII AAKAVAFLEN LQPEYKSYIK QVISNAKALA SSLQERGYDI LTGGTDNHIV LVDLCKDGIT GKLAANSLDR AGITCNKNAI PFDETSPFIT SGIRLGTPAC TTRGFKEKDF VLVGHMVADI LDGLKNNEDN GKAEQKV
Validated NT Sequence
agtacttttt gctcggcttt accattatct tcattatttt ttaagccgtc taaaatatct gctaccatat gaccgactaa tacaaaatcc ttttctttaa atcctctagt agtgcatgcc ggagtgccaa gacgaatacc tgaagtgata aaaggcgaag tttcatcaaa ggggatagca ttcttattac atgtaatccc tgctctatcc aaagaattag cagcaagctt tccggtaatc ccgtctttac acaaatccac taaaacaata tgattatcgg taccgcctgt taatatatca tatcctcttt cttgcaagct acttgctaaa gctttagcat tacttattac ctgcttaata tagcttttat attcaggctg caaattttct aaaaaagcta cggcttttgc ggcgattata tgcattaacg gaccgccttg tagtcctgga aataatgcag aatttatctt tttgcctatc tcttcgtcgt tagataaaat taaaccgcct cgcggtcctc ggagcgtttt gtgagtggta gaggtaacag catgagtgta aggaataggg ctttgatgct cgcctgtggc aacaagtccg gcaatatgag caatatctgc cataaagtaa gctcctactt tatccgctat ttctctaaat cttgcaaaat caatattacg aggataagca gaaaaaccgg ctataagtaa ttttggttta tgcaaatccg ctaagcgttc aatctcatca taatcgatta aataagtctc tttatttacg ctataagaaa cggcgttaaa ccatttaccg gacatattcg gggctgcacc gtgcgttaga tgcccgccgc tatctaaaga cataccaaga attgtatcac caggctgtaa taaagcaaga tacacagctt gatttgcttg tgaaccggaa tgagactgca catttgcata tttacagtta aagagttttt ttactcgctc tatagctaaa ttctcagctt tatcgacttc ctcacaaccg ttatagaaac gcttacctga atacccttcc gcatatttat tagtcagaat cgatccttga gcttcaagca ctgcgggact tacgaaattc tctgatgcaa taagctcaat tacgctattt tgacgcagct tttcatgctt gattatttca tcaatttctt tatccgtttc gtgtaagtga ttattaaaa
Expected Protein Sequence
MAHHHHHHMN IFNNHLHETD KEIDEIIKHE KLRQNSVIEL IASENFVSPA VLEAQGSILT NKYAEGYSGK RFYNGCEEVD KAENLAIERV KKLFNCKYAN VQSHSGSQAN QAVYLALLQP GDTILGMSLD SGGHLTHGAA PNMSGKWFNA VSYSVNKETY LIDYDEIERL ADLHKPKLLI AGFSAYPRNI DFARFREIAD KVGAYFMADI AHIAGLVATG EHQSPIPYTH AVTSTTHKTL RGPRGGLILS NDEEIGKKIN SALFPGLQGG PLMHIIAAKA VAFLENLQPE YKSYIKQVIS NAKALASSLQ ERGYDILTGG TDNHIVLVDL CKDGITGKLA ANSLDRAGIT CNKNAIPFDE TSPFITSGIR LGTPACTTRG FKEKDFVLVG HMVADILDGL KNNEDNGKAE QKVLTEVTKL IKLFPFYTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata tttttaataa tcacttacac gaaacggata aagaaattga tgaaataatc aagcatgaaa agctgcgtca aaatagcgta attgagctta ttgcatcaga gaatttcgta agtcccgcag tgcttgaagc tcaaggatcg attctgacta ataaatatgc ggaagggtat tcaggtaagc gtttctataa cggttgtgag gaagtcgata aagctgagaa tttagctata gagcgagtaa aaaaactctt taactgtaaa tatgcaaatg tgcagtctca ttccggttca caagcaaatc aagctgtgta tcttgcttta ttacagcctg gtgatacaat tcttggtatg tctttagata gcggcgggca tctaacgcac ggtgcagccc cgaatatgtc cggtaaatgg tttaacgccg tttcttatag cgtaaataaa gagacttatt taatcgatta tgatgagatt gaacgcttag cggatttgca taaaccaaaa ttacttatag ccggtttttc tgcttatcct cgtaatattg attttgcaag atttagagaa atagcggata aagtaggagc ttactttatg gcagatattg ctcatattgc cggacttgtt gccacaggcg agcatcaaag ccctattcct tacactcatg ctgttacctc taccactcac aaaacgctcc gaggaccgcg aggcggttta attttatcta acgacgaaga gataggcaaa aagataaatt ctgcattatt tccaggacta caaggcggtc cgttaatgca tataatcgcc gcaaaagccg tagctttttt agaaaatttg cagcctgaat ataaaagcta tattaagcag gtaataagta atgctaaagc tttagcaagt agcttgcaag aaagaggata tgatatatta acaggcggta ccgataatca tattgtttta gtggatttgt gtaaagacgg gattaccgga aagcttgctg ctaattcttt ggatagagca gggattacat gtaataagaa tgctatcccc tttgatgaaa cttcgccttt tatcacttca ggtattcgtc ttggcactcc ggcatgcact actagaggat ttaaagaaaa ggattttgta ttagtcggtc atatggtagc agatatttta gacggcttaa aaaataatga agataatggt aaagccgagc aaaaagtact aactgaagta acaaaactaa ttaaattatt tccgttttat actcggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for RifeA.00008.a.B1.PS01658
PURIFICATION DATe: 10/17/2012
CONCENTRATION: 48mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 66
Protocol Notes
notes unavailable
Validated AA Sequence
MSLDSGGHLT HGAAPNMSGK WFNAVSYSVN KETYLIDYDE IERLADLHKP KLLIAGFSAY PRNIDFARFR EIADKVGAYF MADIAHIAGL VATGEHQSPI PYTHAVTSTT HKTLRGPRGG LILSNDEEIG KKINSALFPG LQGGPLMHII AAKAVAFLEN LQPEYKSYIK QVISNAKALA SSLQERGYDI LTGGTDNHIV LVDLCKDGIT GKLAANSLDR AGITCNKNAI PFDETSPFIT SGIRLGTPAC TTRGFKEKDF VLVGHMVADI LDGLKNNEDN GKAEQKV
Validated NT Sequence
agtacttttt gctcggcttt accattatct tcattatttt ttaagccgtc taaaatatct gctaccatat gaccgactaa tacaaaatcc ttttctttaa atcctctagt agtgcatgcc ggagtgccaa gacgaatacc tgaagtgata aaaggcgaag tttcatcaaa ggggatagca ttcttattac atgtaatccc tgctctatcc aaagaattag cagcaagctt tccggtaatc ccgtctttac acaaatccac taaaacaata tgattatcgg taccgcctgt taatatatca tatcctcttt cttgcaagct acttgctaaa gctttagcat tacttattac ctgcttaata tagcttttat attcaggctg caaattttct aaaaaagcta cggcttttgc ggcgattata tgcattaacg gaccgccttg tagtcctgga aataatgcag aatttatctt tttgcctatc tcttcgtcgt tagataaaat taaaccgcct cgcggtcctc ggagcgtttt gtgagtggta gaggtaacag catgagtgta aggaataggg ctttgatgct cgcctgtggc aacaagtccg gcaatatgag caatatctgc cataaagtaa gctcctactt tatccgctat ttctctaaat cttgcaaaat caatattacg aggataagca gaaaaaccgg ctataagtaa ttttggttta tgcaaatccg ctaagcgttc aatctcatca taatcgatta aataagtctc tttatttacg ctataagaaa cggcgttaaa ccatttaccg gacatattcg gggctgcacc gtgcgttaga tgcccgccgc tatctaaaga cataccaaga attgtatcac caggctgtaa taaagcaaga tacacagctt gatttgcttg tgaaccggaa tgagactgca catttgcata tttacagtta aagagttttt ttactcgctc tatagctaaa ttctcagctt tatcgacttc ctcacaaccg ttatagaaac gcttacctga atacccttcc gcatatttat tagtcagaat cgatccttga gcttcaagca ctgcgggact tacgaaattc tctgatgcaa taagctcaat tacgctattt tgacgcagct tttcatgctt gattatttca tcaatttctt tatccgtttc gtgtaagtga ttattaaaa
Expressed Protein Sequence
MAHHHHHHMN IFNNHLHETD KEIDEIIKHE KLRQNSVIEL IASENFVSPA VLEAQGSILT NKYAEGYSGK RFYNGCEEVD KAENLAIERV KKLFNCKYAN VQSHSGSQAN QAVYLALLQP GDTILGMSLD SGGHLTHGAA PNMSGKWFNA VSYSVNKETY LIDYDEIERL ADLHKPKLLI AGFSAYPRNI DFARFREIAD KVGAYFMADI AHIAGLVATG EHQSPIPYTH AVTSTTHKTL RGPRGGLILS NDEEIGKKIN SALFPGLQGG PLMHIIAAKA VAFLENLQPE YKSYIKQVIS NAKALASSLQ ERGYDILTGG TDNHIVLVDL CKDGITGKLA ANSLDRAGIT CNKNAIPFDE TSPFITSGIR LGTPACTTRG FKEKDFVLVG HMVADILDGL KNNEDNGKAE QKVLTEVTKL IKLFPFYTR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA TTTTTAATAA TCACTTACAC GAAACGGATA AAGAAATTGA TGAAATAATC AAGCATGAAA AGCTGCGTCA AAATAGCGTA ATTGAGCTTA TTGCATCAGA GAATTTCGTA AGTCCCGCAG TGCTTGAAGC TCAAGGATCG ATTCTGACTA ATAAATATGC GGAAGGGTAT TCAGGTAAGC GTTTCTATAA CGGTTGTGAG GAAGTCGATA AAGCTGAGAA TTTAGCTATA GAGCGAGTAA AAAAACTCTT TAACTGTAAA TATGCAAATG TGCAGTCTCA TTCCGGTTCA CAAGCAAATC AAGCTGTGTA TCTTGCTTTA TTACAGCCTG GTGATACAAT TCTTGGTATG TCTTTAGATA GCGGCGGGCA TCTAACGCAC GGTGCAGCCC CGAATATGTC CGGTAAATGG TTTAACGCCG TTTCTTATAG CGTAAATAAA GAGACTTATT TAATCGATTA TGATGAGATT GAACGCTTAG CGGATTTGCA TAAACCAAAA TTACTTATAG CCGGTTTTTC TGCTTATCCT CGTAATATTG ATTTTGCAAG ATTTAGAGAA ATAGCGGATA AAGTAGGAGC TTACTTTATG GCAGATATTG CTCATATTGC CGGACTTGTT GCCACAGGCG AGCATCAAAG CCCTATTCCT TACACTCATG CTGTTACCTC TACCACTCAC AAAACGCTCC GAGGACCGCG AGGCGGTTTA ATTTTATCTA ACGACGAAGA GATAGGCAAA AAGATAAATT CTGCATTATT TCCAGGACTA CAAGGCGGTC CGTTAATGCA TATAATCGCC GCAAAAGCCG TAGCTTTTTT AGAAAATTTG CAGCCTGAAT ATAAAAGCTA TATTAAGCAG GTAATAAGTA ATGCTAAAGC TTTAGCAAGT AGCTTGCAAG AAAGAGGATA TGATATATTA ACAGGCGGTA CCGATAATCA TATTGTTTTA GTGGATTTGT GTAAAGACGG GATTACCGGA AAGCTTGCTG CTAATTCTTT GGATAGAGCA GGGATTACAT GTAATAAGAA TGCTATCCCC TTTGATGAAA CTTCGCCTTT TATCACTTCA GGTATTCGTC TTGGCACTCC GGCATGCACT ACTAGAGGAT TTAAAGAAAA GGATTTTGTA TTAGTCGGTC ATATGGTAGC AGATATTTTA GACGGCTTAA AAAATAATGA AGATAATGGT AAAGCCGAGC AAAAAGTACT AACTGAAGTA ACAAAACTAA TTAAATTATT TCCGTTTTAT ACTCGGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T