RicoA.17987.a
DNA polymerase III subunit beta (EC 2.7.7.7)
| CENTER ID: | RicoA.17987.a |
| ORGANISM: | Rickettsia conorii str. Malish 7 |
| ASSOCIATED DISEASE: | Boutonneuse fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RicoA.17987.a.B1.GE41133 | full length | 1 | 379 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RicoA.17987.a.B1.PW38227 | full length | 1 | 379 |
Structures
5W7Z
DEPOSITED: 6/21/2017
DETERMINATION: XRay
CLONE: RicoA.17987.a.B1.GE41133
PROTEIN: RicoA.17987.a.B1.PW38227
6DM6
DEPOSITED: 6/4/2018
DETERMINATION: XRay
CLONE: RicoA.17987.a.B1.GE41133
PROTEIN: RicoA.17987.a.B1.PW38227
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272944.4.peg.665 |
| UniProt: | Q92I37 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKLIVETKT LVQSLGFASS VVEKRNVIPE YANIKLSAKD GNLELSSTNM DLYLSQKIAV QVVSEGECTV STKTLNDIVR KLPDSELTLT DLGTTGLEIK GKNCKFNLFT LPVSSFPAMD SINPEASFKI SCTDFAKIIE STKFSISLDE TRYNLNGVYL HIKDKEFCSA STDGHRLSIS WVTLEKQIKN FGVILPQKSA EEILKIVKDP KNINEDIEIL LSSNKIKFIC NENTSMLSKL IDGTFPDYST FIPESSSSKL VINRKMFADS IERIAIITVE KFRAVKLSLS RETLEISAVG EARGNAKEVI NSSQDKESFY EYNSDESLAI GFNPQYLEDV LKAVKSDVVE LYFSDVSAPV LIKFPENPKD IFVVMPVKV
NT Sequence
atgttaaaat taatagttga aacaaaaaca ttagttcagt ccctagggtt tgcaagttct gttgttgaaa aacgtaatgt catacctgaa tatgcaaaca ttaaattatc ggcaaaagac ggcaatctag agcttagttc tactaatatg gatttgtact taagtcaaaa aatagcagta caggtagtaa gtgaaggtga atgtaccgtt tctacgaaaa ctctaaatga tatagtccga aaacttccgg attccgagct tacattaaca gatctcggta caacgggact tgagatcaaa ggaaaaaatt gtaaatttaa tttatttact ttgccggtta gttcttttcc tgcaatggat agtattaatc ctgaagcaag ttttaaaatt tcctgcacgg attttgctaa aataatcgaa tcgacaaaat tttcgatttc tttagatgaa actcgttata atttaaacgg tgtttattta cacataaaag acaaagagtt ttgttcggca agtaccgatg ggcatagact ttcgatttca tgggtaacat tagaaaaaca aataaagaat tttggagtta tattacccca aaaaagtgca gaggaaattt taaaaatcgt taaagatcct aaaaatataa atgaagatat agaaatttta ttaagtagca ataagattaa atttatatgt aacgaaaata ctagtatgct atcaaagctt atagacggta ctttccctga ttatagtact tttattccgg aaagcagcag ctcaaaatta gtaattaatc gaaaaatgtt tgcagatagt atagaaagga tagcgataat aaccgttgaa aaatttagag cagtaaaatt atcgttatct cgtgaaacct tagagattag tgccgtcggt gaagcaagag gtaacgcaaa agaggttatt aattcttcac aagataagga aagtttttat gaatataata gtgatgagtc tttagctata ggctttaacc cgcaatattt ggaagatgtc ttaaaagcag tgaaatcgga tgtagtagaa ctatattttt cagatgtttc agcaccggta cttattaaat ttcctgagaa tcctaaagat atttttgtcg tcatgcctgt taaggtgtaa
Details for RicoA.17987.a.B1.GE41133
| HARVESTED ON: | 2/8/2017 |
| SEQUENCED ON: | 2/13/2017 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHML KLIVETKTLV QSLGFASSVV EKRNVIPEYA NIKLSAKDGN LELSSTNMDL YLSQKIAVQV VSEGECTVST KTLNDIVRKL PDSELTLTDL GTTGLEIKGK NCKFNLFTLP VSSFPAMDSI NPEASFKISC TDFAKIIEST KFSISLDETR YNLNGVYLHI KDKEFCSAST DGHRLSISWV TLEKQIKNFG VILPQKSAEE ILKIVKDPKN INEDIEILLS SNKIKFICNE NTSMLSKLID GTFPDYSTFI PESSSSKLVI NRKMFADSIE RIAIITVEKF RAVKLSLSRE TLEISAVGEA RGNAKEVINS SQDKESFYEY NSDESLAIGF NPQYLEDVLK AVKSDVVELY FSDVSAPVLI KFPENPKDI
Validated NT Sequence
aaaaatatct ttaggattct caggaaattt aataagtacc ggtgctgaaa catctgaaaa atatagttct actacatccg atttcactgc ttttaagaca tcttccaaat attgcgggtt aaagcctata gctaaagact catcactatt atattcataa aaactttcct tatcttgtga agaattaata acctcttttg cgttacctct tgcttcaccg acggcactaa tctctaaggt ttcacgagat aacgataatt ttactgctct aaatttttca acggttatta tcgctatcct ttctatacta tctgcaaaca tttttcgatt aattactaat tttgagctgc tgctttccgg aataaaagta ctataatcag ggaaagtacc gtctataagc tttgatagca tactagtatt ttcgttacat ataaatttaa tcttattgct acttaataaa atttctatat cttcatttat atttttagga tctttaacga tttttaaaat ttcctctgca cttttttggg gtaatataac tccaaaattc tttatttgtt tttctaatgt tacccatgaa atcgaaagtc tatgcccatc ggtacttgcc gaacaaaact ctttgtcttt tatgtgtaaa taaacaccgt ttaaattata acgagtttca tctaaagaaa tcgaaaattt tgtcgattcg attattttag caaaatccgt gcaggaaatt ttaaaacttg cttcaggatt aatactatcc attgcaggaa aagaactaac cggcaaagta aataaattaa atttacaatt ttttcctttg atctcaagtc ccgttgtacc gagatctgtt aatgtaagct cggaatccgg aagttttcgg actatatcat ttagagtttt cgtagaaacg gtacattcac cttcacttac tacctgtact gctatttttt gacttaagta caaatccata ttagtagaac taagctctag attgccgtct tttgccgata atttaatgtt tgcatattca ggtatgacat tacgtttttc aacaacagaa cttgcaaacc ctagggactg aactaatgtt tttgtttcaa ctattaattt taacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHML KLIVETKTLV QSLGFASSVV EKRNVIPEYA NIKLSAKDGN LELSSTNMDL YLSQKIAVQV VSEGECTVST KTLNDIVRKL PDSELTLTDL GTTGLEIKGK NCKFNLFTLP VSSFPAMDSI NPEASFKISC TDFAKIIEST KFSISLDETR YNLNGVYLHI KDKEFCSAST DGHRLSISWV TLEKQIKNFG VILPQKSAEE ILKIVKDPKN INEDIEILLS SNKIKFICNE NTSMLSKLID GTFPDYSTFI PESSSSKLVI NRKMFADSIE RIAIITVEKF RAVKLSLSRE TLEISAVGEA RGNAKEVINS SQDKESFYEY NSDESLAIGF NPQYLEDVLK AVKSDVVELY FSDVSAPVLI KFPENPKDIF VVMPVKV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa aattaatagt tgaaacaaaa acattagttc agtccctagg gtttgcaagt tctgttgttg aaaaacgtaa tgtcatacct gaatatgcaa acattaaatt atcggcaaaa gacggcaatc tagagcttag ttctactaat atggatttgt acttaagtca aaaaatagca gtacaggtag taagtgaagg tgaatgtacc gtttctacga aaactctaaa tgatatagtc cgaaaacttc cggattccga gcttacatta acagatctcg gtacaacggg acttgagatc aaaggaaaaa attgtaaatt taatttattt actttgccgg ttagttcttt tcctgcaatg gatagtatta atcctgaagc aagttttaaa atttcctgca cggattttgc taaaataatc gaatcgacaa aattttcgat ttctttagat gaaactcgtt ataatttaaa cggtgtttat ttacacataa aagacaaaga gttttgttcg gcaagtaccg atgggcatag actttcgatt tcatgggtaa cattagaaaa acaaataaag aattttggag ttatattacc ccaaaaaagt gcagaggaaa ttttaaaaat cgttaaagat cctaaaaata taaatgaaga tatagaaatt ttattaagta gcaataagat taaatttata tgtaacgaaa atactagtat gctatcaaag cttatagacg gtactttccc tgattatagt acttttattc cggaaagcag cagctcaaaa ttagtaatta atcgaaaaat gtttgcagat agtatagaaa ggatagcgat aataaccgtt gaaaaattta gagcagtaaa attatcgtta tctcgtgaaa ccttagagat tagtgccgtc ggtgaagcaa gaggtaacgc aaaagaggtt attaattctt cacaagataa ggaaagtttt tatgaatata atagtgatga gtctttagct ataggcttta acccgcaata tttggaagat gtcttaaaag cagtgaaatc ggatgtagta gaactatatt tttcagatgt ttcagcaccg gtacttatta aatttcctga gaatcctaaa gatatttttg tcgtcatgcc tgttaaggtg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for RicoA.17987.a.B1.PW38227
| PURIFICATION DATe: | 3/14/2017 |
| CONCENTRATION: | 27.5mg/ml |
| OBSERVED MW: | 41kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML KLIVETKTLV QSLGFASSVV EKRNVIPEYA NIKLSAKDGN LELSSTNMDL YLSQKIAVQV VSEGECTVST KTLNDIVRKL PDSELTLTDL GTTGLEIKGK NCKFNLFTLP VSSFPAMDSI NPEASFKISC TDFAKIIEST KFSISLDETR YNLNGVYLHI KDKEFCSAST DGHRLSISWV TLEKQIKNFG VILPQKSAEE ILKIVKDPKN INEDIEILLS SNKIKFICNE NTSMLSKLID GTFPDYSTFI PESSSSKLVI NRKMFADSIE RIAIITVEKF RAVKLSLSRE TLEISAVGEA RGNAKEVINS SQDKESFYEY NSDESLAIGF NPQYLEDVLK AVKSDVVELY FSDVSAPVLI KFPENPKDI
Validated NT Sequence
aaaaatatct ttaggattct caggaaattt aataagtacc ggtgctgaaa catctgaaaa atatagttct actacatccg atttcactgc ttttaagaca tcttccaaat attgcgggtt aaagcctata gctaaagact catcactatt atattcataa aaactttcct tatcttgtga agaattaata acctcttttg cgttacctct tgcttcaccg acggcactaa tctctaaggt ttcacgagat aacgataatt ttactgctct aaatttttca acggttatta tcgctatcct ttctatacta tctgcaaaca tttttcgatt aattactaat tttgagctgc tgctttccgg aataaaagta ctataatcag ggaaagtacc gtctataagc tttgatagca tactagtatt ttcgttacat ataaatttaa tcttattgct acttaataaa atttctatat cttcatttat atttttagga tctttaacga tttttaaaat ttcctctgca cttttttggg gtaatataac tccaaaattc tttatttgtt tttctaatgt tacccatgaa atcgaaagtc tatgcccatc ggtacttgcc gaacaaaact ctttgtcttt tatgtgtaaa taaacaccgt ttaaattata acgagtttca tctaaagaaa tcgaaaattt tgtcgattcg attattttag caaaatccgt gcaggaaatt ttaaaacttg cttcaggatt aatactatcc attgcaggaa aagaactaac cggcaaagta aataaattaa atttacaatt ttttcctttg atctcaagtc ccgttgtacc gagatctgtt aatgtaagct cggaatccgg aagttttcgg actatatcat ttagagtttt cgtagaaacg gtacattcac cttcacttac tacctgtact gctatttttt gacttaagta caaatccata ttagtagaac taagctctag attgccgtct tttgccgata atttaatgtt tgcatattca ggtatgacat tacgtttttc aacaacagaa cttgcaaacc ctagggactg aactaatgtt tttgtttcaa ctattaattt taacatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHML KLIVETKTLV QSLGFASSVV EKRNVIPEYA NIKLSAKDGN LELSSTNMDL YLSQKIAVQV VSEGECTVST KTLNDIVRKL PDSELTLTDL GTTGLEIKGK NCKFNLFTLP VSSFPAMDSI NPEASFKISC TDFAKIIEST KFSISLDETR YNLNGVYLHI KDKEFCSAST DGHRLSISWV TLEKQIKNFG VILPQKSAEE ILKIVKDPKN INEDIEILLS SNKIKFICNE NTSMLSKLID GTFPDYSTFI PESSSSKLVI NRKMFADSIE RIAIITVEKF RAVKLSLSRE TLEISAVGEA RGNAKEVINS SQDKESFYEY NSDESLAIGF NPQYLEDVLK AVKSDVVELY FSDVSAPVLI KFPENPKDIF VVMPVKV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAA AATTAATAGT TGAAACAAAA ACATTAGTTC AGTCCCTAGG GTTTGCAAGT TCTGTTGTTG AAAAACGTAA TGTCATACCT GAATATGCAA ACATTAAATT ATCGGCAAAA GACGGCAATC TAGAGCTTAG TTCTACTAAT ATGGATTTGT ACTTAAGTCA AAAAATAGCA GTACAGGTAG TAAGTGAAGG TGAATGTACC GTTTCTACGA AAACTCTAAA TGATATAGTC CGAAAACTTC CGGATTCCGA GCTTACATTA ACAGATCTCG GTACAACGGG ACTTGAGATC AAAGGAAAAA ATTGTAAATT TAATTTATTT ACTTTGCCGG TTAGTTCTTT TCCTGCAATG GATAGTATTA ATCCTGAAGC AAGTTTTAAA ATTTCCTGCA CGGATTTTGC TAAAATAATC GAATCGACAA AATTTTCGAT TTCTTTAGAT GAAACTCGTT ATAATTTAAA CGGTGTTTAT TTACACATAA AAGACAAAGA GTTTTGTTCG GCAAGTACCG ATGGGCATAG ACTTTCGATT TCATGGGTAA CATTAGAAAA ACAAATAAAG AATTTTGGAG TTATATTACC CCAAAAAAGT GCAGAGGAAA TTTTAAAAAT CGTTAAAGAT CCTAAAAATA TAAATGAAGA TATAGAAATT TTATTAAGTA GCAATAAGAT TAAATTTATA TGTAACGAAA ATACTAGTAT GCTATCAAAG CTTATAGACG GTACTTTCCC TGATTATAGT ACTTTTATTC CGGAAAGCAG CAGCTCAAAA TTAGTAATTA ATCGAAAAAT GTTTGCAGAT AGTATAGAAA GGATAGCGAT AATAACCGTT GAAAAATTTA GAGCAGTAAA ATTATCGTTA TCTCGTGAAA CCTTAGAGAT TAGTGCCGTC GGTGAAGCAA GAGGTAACGC AAAAGAGGTT ATTAATTCTT CACAAGATAA GGAAAGTTTT TATGAATATA ATAGTGATGA GTCTTTAGCT ATAGGCTTTA ACCCGCAATA TTTGGAAGAT GTCTTAAAAG CAGTGAAATC GGATGTAGTA GAACTATATT TTTCAGATGT TTCAGCACCG GTACTTATTA AATTTCCTGA GAATCCTAAA GATATTTTTG TCGTCATGCC TGTTAAGGTG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT