RicoA.17834.a

Putative uncharacterized protein

CENTER ID: RicoA.17834.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.17834.a.B1.GE35306 Full length( RicoA.17834.a ) 1 554
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.17834.a.B1.PW35934 Full length( RicoA.17834.a ) 1 554

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.1400
RefSeq: NP_360858.1
UniProt: Q92GA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPKATNSSS TPNGHDKMAK KTHSAQSVVN GAVSDHNTYD EIPYESYPYA LTHPYHLSTL ATLFGVNAPE VENAKILELG CAAGGNLIPH AVLYPKAYFV GVDLSKVQID EANKNVKALG LKNIEFHHCS ITDINDSFGK FDYIICHGVI SWVPKTVRDK IFEVCNKNLS PNGIAYISYN TLPGWNMVRT IRDMMMYHSS SFANVRDRIA QSRLLLEFVK DSLENSKTPY AEALKTEAGL LAKQTDHYLR HDHLEEENAQ FYFHEFMNEA RKHNLQYLAD CNLSTMYLGN MPPKVVEQLK AVNDIVRTEQ YMDFITNRRF RTTLLCHSDV KINRNINNDD ITKFNIIFNI VPEKPLKEID LNNASENLKF FLNGNQDSNL TTSSPYMKAI LYTFSENLNN PLSFEKITTE ANKKLHNTKL NEIKAELLNN AMKLVLQGYI SITNQKHRNN PELDKPKTTK MVIHQATHTP SMWVTNLKHE PIGVNFFEKF ALRYMDGKHD KKAIIEAVLG HVEKGELTLS KEGQKVENKE EIRKELESLF IPMIKKFSSN ALLV
NT Sequence
ATGTCTCCAA AAGCTACTAA CTCTTCTTCT ACCCCTAACG GTCATGATAA GATGGCAAAA AAAACACATT CCGCACAATC CGTTGTTAAC GGTGCGGTTT CAGATCATAA CACTTATGAT GAAATACCGT ATGAAAGCTA CCCATATGCT CTTACACATC CTTATCACTT AAGTACACTT GCAACTCTTT TCGGTGTAAA TGCTCCTGAA GTCGAGAATG CAAAAATATT AGAGCTTGGT TGTGCAGCAG GCGGTAATTT AATACCACAT GCAGTTCTTT ATCCAAAAGC TTATTTTGTT GGGGTTGATT TGTCTAAGGT ACAAATTGAC GAAGCAAATA AAAATGTTAA AGCACTAGGA TTAAAAAATA TAGAGTTCCA TCATTGTTCG ATAACTGATA TTAACGATTC TTTCGGTAAG TTTGATTATA TAATTTGCCA TGGCGTAATT TCTTGGGTAC CAAAAACCGT TAGAGATAAA ATTTTTGAAG TTTGTAATAA AAATCTTAGT CCAAACGGAA TAGCATATAT TAGCTATAAT ACCCTCCCTG GCTGGAATAT GGTCCGTACT ATTAGAGATA TGATGATGTA TCATTCTAGC TCATTTGCAA ATGTACGGGA TAGAATAGCT CAATCTAGAT TGTTACTAGA ATTTGTTAAG GATAGCTTAG AAAACTCTAA AACTCCTTAT GCAGAAGCAT TAAAAACCGA AGCAGGGCTA CTTGCTAAAC AAACCGATCA TTATTTACGT CATGATCATC TAGAAGAAGA AAATGCTCAA TTCTACTTCC ATGAATTTAT GAATGAAGCA AGAAAGCACA ATTTACAATA TTTAGCCGAT TGTAATCTTT CGACTATGTA TCTCGGTAAT ATGCCGCCGA AAGTAGTAGA GCAGTTAAAA GCGGTAAACG ATATAGTTAG AACCGAACAA TATATGGATT TCATTACGAA TCGTCGTTTT AGAACCACTT TACTATGTCA TAGTGATGTG AAAATTAATA GAAATATTAA CAATGATGAT ATAACGAAAT TTAATATAAT CTTTAATATA GTGCCTGAAA AACCACTCAA AGAAATAGAT CTTAATAATG CTTCCGAAAA TTTAAAATTC TTCTTAAACG GCAATCAAGA CTCTAACTTA ACAACTAGTT CGCCTTATAT GAAAGCTATT TTATACACTT TCAGCGAAAA TCTAAATAAT CCGTTAAGCT TTGAGAAAAT AACCACTGAA GCTAATAAAA AGCTACATAA TACTAAATTA AACGAAATCA AAGCTGAGCT TTTAAATAAT GCAATGAAGC TAGTACTACA AGGTTATATT AGTATTACAA ATCAGAAGCA TCGAAATAAC CCTGAGCTTG ATAAGCCTAA AACAACAAAA ATGGTGATAC ATCAAGCAAC CCATACACCT TCTATGTGGG TTACAAATTT AAAACATGAA CCGATCGGCG TTAATTTCTT TGAGAAGTTT GCACTTAGAT ATATGGATGG TAAACATGAT AAAAAAGCAA TCATTGAGGC TGTACTTGGT CATGTAGAAA AAGGCGAGCT AACTTTAAGT AAAGAAGGTC AAAAAGTAGA AAACAAGGAA GAAATACGCA AAGAACTAGA AAGCTTATTT ATTCCGATGA TCAAGAAATT TTCTTCTAAT GCTTTGTTGG TG
Details for RicoA.17834.a.B1.GE35306
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Validated AA Sequence
MAKKTHSAQS VVNGAVSDHN TYDEIPYESY PYALTHPYHL STLATLFGVN APEVENAKIL ELGCAAGGNL IPHAVLYPKA YFVGVDLSKV QIDEANKNVK ALGLKNIEFH HCSITDINDS FGKFDYIICH GVISWVPKTV RDKIFEVCNK NLSPNGIAYI SYNTLPGWNM VRTIRDMMMY HSSSFANVRD RIAQSRLXLE FVKDSLENSK TPYAEALKTE AGLLAKQTDH YLRHDHLEEE NAQFYFXEFM NEARKHNLQY LADCNLSTMY LGNMPPKVVE QLKAVNDIVR XEQYMDFITN RRFRTTLLCH SDVKINRNIN NDDITKFNII FNIVPEKPLK EIDLNNASEN LKFFLNGNQD SNLTTSSPYM KAILYTFSEN LNNPLSFEKI TTEANKKLHN TKLNEIKAEL LNNAMKLVLQ GYISITNQKH RNNPELDKPK TTKMVIHQAT HTPSMWVTNL KHEPIGVNFF EKFALRYMDG KHDKKAIIEA VLGHVEKGEL TLSKEGQKVE NKEEIRKELE SLFIPM
Validated NT Sequence
gatcatcgga ataaataagc tttctagttc tttgcgtatt tcttccttgt tttctacttt ttgaccttct ttacttaaag ttagctcgcc tttttctaca tgaccaagta cagcctcaat gattgctttt ttatcatgtt taccatccat atatctaagt gcaaacttct caaagaaatt aacgccgatc ggttcatgtt ttaaatttgt aacccacata gaaggtgtat gggttgcttg atgtatcacc atttttgttg ttttaggctt atcaagctca gggttatttc gatgcttctg atttgtaata ctaatataac cttgtagtac tagcttcatt gcattattta aaagctcagc tttgatttcg tttaatttag tattatgtag ctttttatta gcttcagtgg ttattttctc aaagcttaac ggattattta gattttcgct gaaagtgtat aaaatagctt tcatataagg cgaactagtt gttaagttag agtcttgatt gccgtttaag aagaatttta aattttcgga agcattatta agatctattt ctttgagtgg tttttcaggc actatattaa agattatatt aaatttcgtt atatcatcat tgttaatatt tctattaatt ttcacatcac tatgacatag taaagtggtt ctaaaacgac gattcgtaat gaaatccata tattgttcgg ntctaactat atcgtttacc gcttttaact gctctactac tttcggcggc atattaccga gatacatagt ngaaagatta caatcggcta aatattgtaa attgtgcttt cttgcttcat tcataaattc ntggaagtag aattgagcat tttcttcttc tagatgatca tgacgtaaat aatgatcggt ttgtttagca agtagccctg cttcggtttt taatgcttct gcataaggag ttttagagtt ttctaagcta tccttaacaa attctagtan caatctagat tgagctattc tatcccgtac atttgcaaat gagctagaat gatacatcat catatctcta atagtacgga ccatattcca gccagggagg gtattatagc taatatatgc tattccgttt ggactaagat ttttattaca aacttcaaaa attttatctc taacggtttt tggtacccaa gaaattacgc catggcaaat tatataatca aacttaccga aagaatcgtt aatatcagtt atcgaacaat gatggaactc tatatttttt aatcctagtg ctttaacatt tttatttgct tcgtcaattt gtaccttaga caaatcaacc ccaacaaaat aagcttttgg ataaagaact gcatgtggta ttaaattacc gcctgctgca caaccaagct ctaatatttt tgcattctcg acttcaggag catttacacc gaaaagagtt gcaagtgtac ttaagtgata aggatgtgta agagcatatg ggtagctttc atacggtatt tcatcataag tgttatgatc tgaaaccgca ccgttaacaa cggattgtgc ggaatgtgtt ttttttgcca tcttatcatg accgttaggg gtagaagaag agttagtagc ttttggagac
Expected Protein Sequence
MAHHHHHHMS PKATNSSSTP NGHDKMAKKT HSAQSVVNGA VSDHNTYDEI PYESYPYALT HPYHLSTLAT LFGVNAPEVE NAKILELGCA AGGNLIPHAV LYPKAYFVGV DLSKVQIDEA NKNVKALGLK NIEFHHCSIT DINDSFGKFD YIICHGVISW VPKTVRDKIF EVCNKNLSPN GIAYISYNTL PGWNMVRTIR DMMMYHSSSF ANVRDRIAQS RLLLEFVKDS LENSKTPYAE ALKTEAGLLA KQTDHYLRHD HLEEENAQFY FHEFMNEARK HNLQYLADCN LSTMYLGNMP PKVVEQLKAV NDIVRTEQYM DFITNRRFRT TLLCHSDVKI NRNINNDDIT KFNIIFNIVP EKPLKEIDLN NASENLKFFL NGNQDSNLTT SSPYMKAILY TFSENLNNPL SFEKITTEAN KKLHNTKLNE IKAELLNNAM KLVLQGYISI TNQKHRNNPE LDKPKTTKMV IHQATHTPSM WVTNLKHEPI GVNFFEKFAL RYMDGKHDKK AIIEAVLGHV EKGELTLSKE GQKVENKEEI RKELESLFIP MIKKFSSNAL LV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc caaaagctac taactcttct tctaccccta acggtcatga taagatggca aaaaaaacac attccgcaca atccgttgtt aacggtgcgg tttcagatca taacacttat gatgaaatac cgtatgaaag ctacccatat gctcttacac atccttatca cttaagtaca cttgcaactc ttttcggtgt aaatgctcct gaagtcgaga atgcaaaaat attagagctt ggttgtgcag caggcggtaa tttaatacca catgcagttc tttatccaaa agcttatttt gttggggttg atttgtctaa ggtacaaatt gacgaagcaa ataaaaatgt taaagcacta ggattaaaaa atatagagtt ccatcattgt tcgataactg atattaacga ttctttcggt aagtttgatt atataatttg ccatggcgta atttcttggg taccaaaaac cgttagagat aaaatttttg aagtttgtaa taaaaatctt agtccaaacg gaatagcata tattagctat aataccctcc ctggctggaa tatggtccgt actattagag atatgatgat gtatcattct agctcatttg caaatgtacg ggatagaata gctcaatcta gattgttact agaatttgtt aaggatagct tagaaaactc taaaactcct tatgcagaag cattaaaaac cgaagcaggg ctacttgcta aacaaaccga tcattattta cgtcatgatc atctagaaga agaaaatgct caattctact tccatgaatt tatgaatgaa gcaagaaagc acaatttaca atatttagcc gattgtaatc tttcgactat gtatctcggt aatatgccgc cgaaagtagt agagcagtta aaagcggtaa acgatatagt tagaaccgaa caatatatgg atttcattac gaatcgtcgt tttagaacca ctttactatg tcatagtgat gtgaaaatta atagaaatat taacaatgat gatataacga aatttaatat aatctttaat atagtgcctg aaaaaccact caaagaaata gatcttaata atgcttccga aaatttaaaa ttcttcttaa acggcaatca agactctaac ttaacaacta gttcgcctta tatgaaagct attttataca ctttcagcga aaatctaaat aatccgttaa gctttgagaa aataaccact gaagctaata aaaagctaca taatactaaa ttaaacgaaa tcaaagctga gcttttaaat aatgcaatga agctagtact acaaggttat attagtatta caaatcagaa gcatcgaaat aaccctgagc ttgataagcc taaaacaaca aaaatggtga tacatcaagc aacccataca ccttctatgt gggttacaaa tttaaaacat gaaccgatcg gcgttaattt ctttgagaag tttgcactta gatatatgga tggtaaacat gataaaaaag caatcattga ggctgtactt ggtcatgtag aaaaaggcga gctaacttta agtaaagaag gtcaaaaagt agaaaacaag gaagaaatac gcaaagaact agaaagctta tttattccga tgatcaagaa attttcttct aatgctttgt tggtgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for RicoA.17834.a.B1.PW35934
PURIFICATION DATe: 6/22/2012
CONCENTRATION: 30.01mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MAKKTHSAQS VVNGAVSDHN TYDEIPYESY PYALTHPYHL STLATLFGVN APEVENAKIL ELGCAAGGNL IPHAVLYPKA YFVGVDLSKV QIDEANKNVK ALGLKNIEFH HCSITDINDS FGKFDYIICH GVISWVPKTV RDKIFEVCNK NLSPNGIAYI SYNTLPGWNM VRTIRDMMMY HSSSFANVRD RIAQSRLXLE FVKDSLENSK TPYAEALKTE AGLLAKQTDH YLRHDHLEEE NAQFYFXEFM NEARKHNLQY LADCNLSTMY LGNMPPKVVE QLKAVNDIVR XEQYMDFITN RRFRTTLLCH SDVKINRNIN NDDITKFNII FNIVPEKPLK EIDLNNASEN LKFFLNGNQD SNLTTSSPYM KAILYTFSEN LNNPLSFEKI TTEANKKLHN TKLNEIKAEL LNNAMKLVLQ GYISITNQKH RNNPELDKPK TTKMVIHQAT HTPSMWVTNL KHEPIGVNFF EKFALRYMDG KHDKKAIIEA VLGHVEKGEL TLSKEGQKVE NKEEIRKELE SLFIPM
Validated NT Sequence
gatcatcgga ataaataagc tttctagttc tttgcgtatt tcttccttgt tttctacttt ttgaccttct ttacttaaag ttagctcgcc tttttctaca tgaccaagta cagcctcaat gattgctttt ttatcatgtt taccatccat atatctaagt gcaaacttct caaagaaatt aacgccgatc ggttcatgtt ttaaatttgt aacccacata gaaggtgtat gggttgcttg atgtatcacc atttttgttg ttttaggctt atcaagctca gggttatttc gatgcttctg atttgtaata ctaatataac cttgtagtac tagcttcatt gcattattta aaagctcagc tttgatttcg tttaatttag tattatgtag ctttttatta gcttcagtgg ttattttctc aaagcttaac ggattattta gattttcgct gaaagtgtat aaaatagctt tcatataagg cgaactagtt gttaagttag agtcttgatt gccgtttaag aagaatttta aattttcgga agcattatta agatctattt ctttgagtgg tttttcaggc actatattaa agattatatt aaatttcgtt atatcatcat tgttaatatt tctattaatt ttcacatcac tatgacatag taaagtggtt ctaaaacgac gattcgtaat gaaatccata tattgttcgg ntctaactat atcgtttacc gcttttaact gctctactac tttcggcggc atattaccga gatacatagt ngaaagatta caatcggcta aatattgtaa attgtgcttt cttgcttcat tcataaattc ntggaagtag aattgagcat tttcttcttc tagatgatca tgacgtaaat aatgatcggt ttgtttagca agtagccctg cttcggtttt taatgcttct gcataaggag ttttagagtt ttctaagcta tccttaacaa attctagtan caatctagat tgagctattc tatcccgtac atttgcaaat gagctagaat gatacatcat catatctcta atagtacgga ccatattcca gccagggagg gtattatagc taatatatgc tattccgttt ggactaagat ttttattaca aacttcaaaa attttatctc taacggtttt tggtacccaa gaaattacgc catggcaaat tatataatca aacttaccga aagaatcgtt aatatcagtt atcgaacaat gatggaactc tatatttttt aatcctagtg ctttaacatt tttatttgct tcgtcaattt gtaccttaga caaatcaacc ccaacaaaat aagcttttgg ataaagaact gcatgtggta ttaaattacc gcctgctgca caaccaagct ctaatatttt tgcattctcg acttcaggag catttacacc gaaaagagtt gcaagtgtac ttaagtgata aggatgtgta agagcatatg ggtagctttc atacggtatt tcatcataag tgttatgatc tgaaaccgca ccgttaacaa cggattgtgc ggaatgtgtt ttttttgcca tcttatcatg accgttaggg gtagaagaag agttagtagc ttttggagac
Expressed Protein Sequence
MAHHHHHHMS PKATNSSSTP NGHDKMAKKT HSAQSVVNGA VSDHNTYDEI PYESYPYALT HPYHLSTLAT LFGVNAPEVE NAKILELGCA AGGNLIPHAV LYPKAYFVGV DLSKVQIDEA NKNVKALGLK NIEFHHCSIT DINDSFGKFD YIICHGVISW VPKTVRDKIF EVCNKNLSPN GIAYISYNTL PGWNMVRTIR DMMMYHSSSF ANVRDRIAQS RLLLEFVKDS LENSKTPYAE ALKTEAGLLA KQTDHYLRHD HLEEENAQFY FHEFMNEARK HNLQYLADCN LSTMYLGNMP PKVVEQLKAV NDIVRTEQYM DFITNRRFRT TLLCHSDVKI NRNINNDDIT KFNIIFNIVP EKPLKEIDLN NASENLKFFL NGNQDSNLTT SSPYMKAILY TFSENLNNPL SFEKITTEAN KKLHNTKLNE IKAELLNNAM KLVLQGYISI TNQKHRNNPE LDKPKTTKMV IHQATHTPSM WVTNLKHEPI GVNFFEKFAL RYMDGKHDKK AIIEAVLGHV EKGELTLSKE GQKVENKEEI RKELESLFIP MIKKFSSNAL LV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTC CAAAAGCTAC TAACTCTTCT TCTACCCCTA ACGGTCATGA TAAGATGGCA AAAAAAACAC ATTCCGCACA ATCCGTTGTT AACGGTGCGG TTTCAGATCA TAACACTTAT GATGAAATAC CGTATGAAAG CTACCCATAT GCTCTTACAC ATCCTTATCA CTTAAGTACA CTTGCAACTC TTTTCGGTGT AAATGCTCCT GAAGTCGAGA ATGCAAAAAT ATTAGAGCTT GGTTGTGCAG CAGGCGGTAA TTTAATACCA CATGCAGTTC TTTATCCAAA AGCTTATTTT GTTGGGGTTG ATTTGTCTAA GGTACAAATT GACGAAGCAA ATAAAAATGT TAAAGCACTA GGATTAAAAA ATATAGAGTT CCATCATTGT TCGATAACTG ATATTAACGA TTCTTTCGGT AAGTTTGATT ATATAATTTG CCATGGCGTA ATTTCTTGGG TACCAAAAAC CGTTAGAGAT AAAATTTTTG AAGTTTGTAA TAAAAATCTT AGTCCAAACG GAATAGCATA TATTAGCTAT AATACCCTCC CTGGCTGGAA TATGGTCCGT ACTATTAGAG ATATGATGAT GTATCATTCT AGCTCATTTG CAAATGTACG GGATAGAATA GCTCAATCTA GATTGTTACT AGAATTTGTT AAGGATAGCT TAGAAAACTC TAAAACTCCT TATGCAGAAG CATTAAAAAC CGAAGCAGGG CTACTTGCTA AACAAACCGA TCATTATTTA CGTCATGATC ATCTAGAAGA AGAAAATGCT CAATTCTACT TCCATGAATT TATGAATGAA GCAAGAAAGC ACAATTTACA ATATTTAGCC GATTGTAATC TTTCGACTAT GTATCTCGGT AATATGCCGC CGAAAGTAGT AGAGCAGTTA AAAGCGGTAA ACGATATAGT TAGAACCGAA CAATATATGG ATTTCATTAC GAATCGTCGT TTTAGAACCA CTTTACTATG TCATAGTGAT GTGAAAATTA ATAGAAATAT TAACAATGAT GATATAACGA AATTTAATAT AATCTTTAAT ATAGTGCCTG AAAAACCACT CAAAGAAATA GATCTTAATA ATGCTTCCGA AAATTTAAAA TTCTTCTTAA ACGGCAATCA AGACTCTAAC TTAACAACTA GTTCGCCTTA TATGAAAGCT ATTTTATACA CTTTCAGCGA AAATCTAAAT AATCCGTTAA GCTTTGAGAA AATAACCACT GAAGCTAATA AAAAGCTACA TAATACTAAA TTAAACGAAA TCAAAGCTGA GCTTTTAAAT AATGCAATGA AGCTAGTACT ACAAGGTTAT ATTAGTATTA CAAATCAGAA GCATCGAAAT AACCCTGAGC TTGATAAGCC TAAAACAACA AAAATGGTGA TACATCAAGC AACCCATACA CCTTCTATGT GGGTTACAAA TTTAAAACAT GAACCGATCG GCGTTAATTT CTTTGAGAAG TTTGCACTTA GATATATGGA TGGTAAACAT GATAAAAAAG CAATCATTGA GGCTGTACTT GGTCATGTAG AAAAAGGCGA GCTAACTTTA AGTAAAGAAG GTCAAAAAGT AGAAAACAAG GAAGAAATAC GCAAAGAACT AGAAAGCTTA TTTATTCCGA TGATCAAGAA ATTTTCTTCT AATGCTTTGT TGGTGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT