SSGCID: Target Page

RicoA.17732.a

Patatin b1

CENTER ID:	RicoA.17732.a
ORGANISM:	Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE:	Boutonneuse fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RicoA.17732.a.B1.GE35304	Full length( RicoA.17732.a )		1	490

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272944.4.peg.1049
RefSeq:	NP_360559.1
UniProt:	Q92H50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVEINNNKIS LALFEGGGVK GNIHLEKLKI MEQITGKSTC EIFDFTGGTS VGGLISILLN LPDPNNPGKP LFSAAQAQEL FEEMAHNIFP VGLTFRKLWS FNGLFSHKFS PEPLVKLLKA YCKDYTLKDL IGDVVVTGYD LNNKQNPLMT FSTIDARQSE ENDYYLSDII QGITAAPGYF PSHHFRNITN TKSHKIIDGG VYANDPTLQT WQLLKENNYN IENALYLSLK EENNDDYQTV CCGGGMLELV KNNMPAKILA ATQQADEKTA QSIFGNKLIE IATYIPEKHS EMSDSSTENL QALKEFAKKS IYGSSTFRNI PYNEKFKEAI DKIITNYNAN NPNDQIVMND FYKELFHINT SNNALDKSFE QEEDIANNED AVKTLIAQAL DSKDIEPGKK ELLADIKEFF NPFIEKNPEA KVDIENFLHE VKNLTLAEIE NGIVTFKEAR LKWQAEQNNL DIFSSCSMEA LKEDMTLEGE EHTDDVQGYL

NT Sequence

ATGGTAGAGA TAAACAACAA TAAGATTAGC TTAGCACTAT TTGAAGGCGG TGGAGTAAAA GGAAATATCC ATTTAGAAAA ATTAAAAATA ATGGAGCAAA TAACCGGTAA GTCTACTTGT GAGATATTCG ATTTTACTGG AGGGACCAGT GTTGGAGGAC TTATTTCTAT TTTGCTTAAT TTACCGGATC CTAATAATCC CGGAAAACCT TTATTCAGTG CAGCGCAAGC TCAAGAACTG TTTGAAGAAA TGGCACACAA TATTTTTCCG GTAGGATTAA CTTTTAGAAA ATTGTGGAGT TTTAATGGCT TATTCAGCCA TAAATTTTCT CCTGAACCAT TAGTAAAATT ATTGAAAGCA TATTGTAAGG ATTATACCCT CAAGGATTTA ATCGGTGATG TTGTAGTTAC AGGTTATGAT CTAAATAATA AACAAAATCC TCTTATGACC TTCTCTACTA TAGATGCTAG ACAAAGTGAA GAGAATGATT ATTACCTTTC AGATATAATA CAAGGCATCA CTGCAGCACC TGGATATTTC CCCAGCCACC ATTTCCGTAA TATAACCAAT ACTAAATCAC ATAAAATTAT TGATGGTGGA GTATATGCTA ATGACCCAAC CTTACAAACA TGGCAATTAT TAAAAGAAAA TAACTATAAC ATAGAAAACG CTTTGTATTT ATCATTAAAA GAAGAAAATA ATGATGATTA CCAAACTGTT TGTTGCGGCG GAGGCATGCT AGAACTAGTG AAAAATAATA TGCCCGCTAA AATATTAGCA GCAACACAAC AAGCTGATGA AAAAACCGCT CAAAGCATTT TTGGTAATAA ATTAATAGAA ATAGCTACTT ATATTCCAGA AAAACATTCT GAGATGAGTG ATTCTAGTAC TGAAAATCTG CAAGCACTAA AAGAATTTGC TAAAAAATCT ATATATGGAA GCTCCACCTT TAGAAATATA CCTTATAATG AAAAGTTTAA AGAAGCTATT GATAAAATAA TAACTAATTA TAATGCAAAT AATCCTAACG ACCAAATTGT TATGAATGAT TTTTACAAAG AGTTATTCCA TATTAATACT TCTAATAACG CACTTGATAA AAGTTTTGAA CAGGAAGAGG ATATAGCAAA CAACGAGGAT GCAGTGAAGA CACTGATAGC TCAAGCTCTA GATTCTAAAG ATATAGAACC TGGGAAAAAA GAATTATTAG CAGATATCAA GGAGTTTTTT AATCCATTCA TCGAAAAAAA TCCAGAAGCA AAAGTAGATA TAGAGAATTT TTTACACGAA GTAAAAAATT TGACATTAGC AGAAATTGAA AACGGTATTG TTACTTTTAA AGAAGCTAGA TTAAAATGGC AAGCAGAGCA GAATAACCTT GATATATTTA GTAGCTGTAG TATGGAAGCC TTAAAAGAGG ATATGACTCT TGAGGGTGAA GAGCATACAG ATGATGTACA AGGTTATCTC

Details for RicoA.17732.a.B1.GE35304

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	62

Validated AA Sequence

Validated NT Sequence

ttnnttactt taagaaggag anataccatg gctcaccacc accaccacca tatggtagag ataaacaaca ataagattag cttagcacta tttgaaggcg gtggagtaaa aggaaatatc catttagaaa aattaaaaat aatggagcaa ataaccggta agtctacttg tgagatattc gattttactg gagggaccag tgttggagga cttatttcta ttttgcttaa tttaccggat cctaataatc ccggaaaacc tttattcagt gcagcgcaag ctcaagaact gtttgaagaa atggcacaca atatttttcc ggtaggatta acttttagaa aattgtggag ttttaatggc ttattcagcc ataaattttc tcctgaacca ttagtaaaat tattgaaagc atattgtaag gattataccc tcaaggattt aatcggtgat gttgtagtta caggttatga tctaaataat aaacaaaatc ctcttatgac cttctctact atagatgcta gacaaagtga agagaatgat tattaccttt cagatataat acaaggcatc actgcagcac ctggatattt ccccagccac catttccgta atataaccaa tactaaatca cataaaatta ttgatggtgg agtatatgct aatgacccaa ccttacaaac atggcaatta ttaaaagaaa ataactataa catagaaaac gctttgtatt tatcattaaa agaagaaaat aatgatgatt accaaactgt ttgttgcggc ggangcatgc tagaactagt gaaaaataat atgcccgcta aaatattagc agcaacacaa caagctgatg aaaaaaccgc tcaaagcatt tttggtaata aattaataga aatagctact tatattccan aaaaacattc tgagatgagt gantctagta ctgaaaatct gcagcactaa ananttgcta aaaatctata tntggnnctn cnnnttanaa tntaccttan atgaaagttn anganctntg nnaaatanan nnnnnnngca anancnanna cnnnnntnng angattttnn anantnnnnn nnnnnctnnn nanncnnnnn naaantnnnn nnannnnnnn nnnnnnnnan nnnnnnnnnn annnnnnnan tnnanannnn a

Expected Protein Sequence

MAHHHHHHMV EINNNKISLA LFEGGGVKGN IHLEKLKIME QITGKSTCEI FDFTGGTSVG GLISILLNLP DPNNPGKPLF SAAQAQELFE EMAHNIFPVG LTFRKLWSFN GLFSHKFSPE PLVKLLKAYC KDYTLKDLIG DVVVTGYDLN NKQNPLMTFS TIDARQSEEN DYYLSDIIQG ITAAPGYFPS HHFRNITNTK SHKIIDGGVY ANDPTLQTWQ LLKENNYNIE NALYLSLKEE NNDDYQTVCC GGGMLELVKN NMPAKILAAT QQADEKTAQS IFGNKLIEIA TYIPEKHSEM SDSSTENLQA LKEFAKKSIY GSSTFRNIPY NEKFKEAIDK IITNYNANNP NDQIVMNDFY KELFHINTSN NALDKSFEQE EDIANNEDAV KTLIAQALDS KDIEPGKKEL LADIKEFFNP FIEKNPEAKV DIENFLHEVK NLTLAEIENG IVTFKEARLK WQAEQNNLDI FSSCSMEALK EDMTLEGEEH TDDVQGYL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtag agataaacaa caataagatt agcttagcac tatttgaagg cggtggagta aaaggaaata tccatttaga aaaattaaaa ataatggagc aaataaccgg taagtctact tgtgagatat tcgattttac tggagggacc agtgttggag gacttatttc tattttgctt aatttaccgg atcctaataa tcccggaaaa cctttattca gtgcagcgca agctcaagaa ctgtttgaag aaatggcaca caatattttt ccggtaggat taacttttag aaaattgtgg agttttaatg gcttattcag ccataaattt tctcctgaac cattagtaaa attattgaaa gcatattgta aggattatac cctcaaggat ttaatcggtg atgttgtagt tacaggttat gatctaaata ataaacaaaa tcctcttatg accttctcta ctatagatgc tagacaaagt gaagagaatg attattacct ttcagatata atacaaggca tcactgcagc acctggatat ttccccagcc accatttccg taatataacc aatactaaat cacataaaat tattgatggt ggagtatatg ctaatgaccc aaccttacaa acatggcaat tattaaaaga aaataactat aacatagaaa acgctttgta tttatcatta aaagaagaaa ataatgatga ttaccaaact gtttgttgcg gcggaggcat gctagaacta gtgaaaaata atatgcccgc taaaatatta gcagcaacac aacaagctga tgaaaaaacc gctcaaagca tttttggtaa taaattaata gaaatagcta cttatattcc agaaaaacat tctgagatga gtgattctag tactgaaaat ctgcaagcac taaaagaatt tgctaaaaaa tctatatatg gaagctccac ctttagaaat ataccttata atgaaaagtt taaagaagct attgataaaa taataactaa ttataatgca aataatccta acgaccaaat tgttatgaat gatttttaca aagagttatt ccatattaat acttctaata acgcacttga taaaagtttt gaacaggaag aggatatagc aaacaacgag gatgcagtga agacactgat agctcaagct ctagattcta aagatataga acctgggaaa aaagaattat tagcagatat caaggagttt tttaatccat tcatcgaaaa aaatccagaa gcaaaagtag atatagagaa ttttttacac gaagtaaaaa atttgacatt agcagaaatt gaaaacggta ttgttacttt taaagaagct agattaaaat ggcaagcaga gcagaataac cttgatatat ttagtagctg tagtatggaa gccttaaaag aggatatgac tcttgagggt gaagagcata cagatgatgt acaaggttat ctctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat