RicoA.17612.a

Uncharacterized protein RC0012

CENTER ID: RicoA.17612.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.17612.a.B1.GE35302 Full length( RicoA.17612.a ) 1 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.17612.a.B1.PW35930 Full length( RicoA.17612.a ) 1 307
RicoA.17612.a.B1.PW36740 Full length( RicoA.17612.a ) 1 307

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.12
RefSeq: NP_359649.1
UniProt: Q92JQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFFSLSYASE KAVIITDYKP VFLPVIAENK KIRIAIRSYL NNEKSYFVLV DPNSFKTEIA LQELVILPTN KIEKENLLKK LSKTPYIKVL NKYSSTPYIQ QNYGATSSMY KVKGQFLTID MCPSSKSFEK DFFKKLVELS IKLNKPIPIA ICVSGLWINK HTEEFLWLLK QQENGYLQIT WVNHSFSHPY FKDKPLEDNF LLSNKDDFEN EVLEAGKILV SYNIAPSPFF RFPGLVSDQT LIEKLKDLGF IPLGSNAWLA KGEKVQNGSF ILVHGNSNEK AGIDLIMPML PELKLLPIEK AFLLHDN
NT Sequence
TTGTTTTTTA GTTTAAGCTA TGCAAGTGAA AAAGCAGTAA TTATAACTGA TTATAAACCG GTATTTTTGC CTGTAATTGC TGAAAATAAA AAAATAAGAA TTGCTATTCG TTCTTATTTA AATAATGAAA AATCATATTT CGTTTTAGTA GATCCGAATT CTTTTAAAAC TGAAATAGCC CTACAAGAGC TGGTAATTTT ACCTACGAAT AAAATAGAAA AAGAAAATCT TCTAAAAAAA TTAAGTAAAA CTCCGTATAT TAAAGTTTTA AATAAATATA GCTCTACACC TTATATACAA CAAAATTACG GTGCTACGAG TAGCATGTAT AAAGTAAAAG GACAGTTCTT GACAATTGAC ATGTGTCCTT CTTCCAAAAG CTTTGAAAAA GATTTTTTTA AAAAGCTCGT AGAATTATCG ATCAAACTAA ATAAACCTAT TCCGATTGCT ATATGTGTTT CAGGATTATG GATTAATAAG CATACAGAAG AATTCTTATG GTTATTAAAA CAACAAGAAA ACGGTTATTT ACAAATAACA TGGGTTAATC ATTCATTTAG TCATCCTTAT TTTAAAGATA AACCTCTTGA AGATAATTTC CTTCTTTCCA ATAAAGATGA TTTTGAAAAT GAAGTATTAG AAGCAGGGAA AATATTAGTA AGTTATAATA TTGCTCCGTC ACCTTTCTTT CGTTTTCCTG GATTAGTTTC CGATCAAACA TTAATAGAAA AATTAAAAGA TTTGGGATTT ATTCCGCTTG GGAGTAATGC ATGGCTTGCT AAAGGCGAAA AAGTTCAAAA TGGTTCTTTT ATATTGGTAC ACGGTAATAG TAACGAAAAA GCCGGAATAG ATTTAATTAT GCCGATGTTA CCGGAATTAA AATTACTTCC TATTGAAAAG GCGTTTTTAT TACATGACAA T
Details for RicoA.17612.a.B1.GE35302
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML FFSLSYASEK AVIITDYKPV FLPVIAENKK IRIAIRSYLN NEKSYFVLVD PNSFKTEIAL QELVILPTNK IEKENLLKKL SKTPYIKVLN KYSSTPYIQQ NYGATSSMYK VKGQFLTIDM CPSSKSFEKD FFKKLVELSI KLNKPIPIAI CVSGLWINKH TEEFLWLLKQ QENGYLQITW VNHSFSHPYF KDKPLEDNFL LSNKDDFENE VLEAGKILVS YNIAPSPFFR FPGLVSDQTL IEKLKDLGFI PLGSNAWLAK GEKVQNGSFI LVHGNSNEKA GIDLIMPMLP ELKLLPIEKA FLLHDN
Validated NT Sequence
tcttactcac ttaattgtca tgtaataaaa acgccttttc aataggaagt aattttaatt ccggtaacat cggcataatt aaatctattc cggctttttc gttactatta ccgtgtacca atataaaaga accattttga actttttcgc ctttagcaag ccatgcatta ctcccaagcg gaataaatcc caaatctttt aatttttcta ttaatgtttg atcggaaact aatccaggaa aacgaaagaa aggtgacgga gcaatattat aacttactaa tattttccct gcttctaata cttcattttc aaaatcatct ttattggaaa gaaggaaatt atcttcaaga ggtttatctt taaaataagg atgactaaat gaatgattaa cccatgttat ttgtaaataa ccgttttctt gttgttttaa taaccataag aattcttctg tatgcttatt aatccataat cctgaaacac atatagcaat cggaataggt ttatttagtt tgatcgataa ttctacgagc tttttaaaaa aatctttttc aaagcttttg gaagaaggac acatgtcaat tgtcaagaac tgtcctttta ctttatacat gctactcgta gcaccgtaat tttgttgtat ataaggtgta gagctatatt tatttaaaac tttaatatac ggagttttac ttaatttttt tagaagattt tctttttcta ttttattcgt aggtaaaatt accagctctt gtagggctat ttcagtttta aaagaattcg gatctactaa aacgaaatat gatttttcat tatttaaata agaacgaata gcaattctta tttttttatt ttcagcaatt acaggcaaaa ataccggttt ataatcagtt ataattactg ctttttcact tgcatagctt aaactaaaaa acaacatatg gtggtggtgg tggtgagcca tggta
Expected Protein Sequence
MAHHHHHHML FFSLSYASEK AVIITDYKPV FLPVIAENKK IRIAIRSYLN NEKSYFVLVD PNSFKTEIAL QELVILPTNK IEKENLLKKL SKTPYIKVLN KYSSTPYIQQ NYGATSSMYK VKGQFLTIDM CPSSKSFEKD FFKKLVELSI KLNKPIPIAI CVSGLWINKH TEEFLWLLKQ QENGYLQITW VNHSFSHPYF KDKPLEDNFL LSNKDDFENE VLEAGKILVS YNIAPSPFFR FPGLVSDQTL IEKLKDLGFI PLGSNAWLAK GEKVQNGSFI LVHGNSNEKA GIDLIMPMLP ELKLLPIEKA FLLHDN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgt tttttagttt aagctatgca agtgaaaaag cagtaattat aactgattat aaaccggtat ttttgcctgt aattgctgaa aataaaaaaa taagaattgc tattcgttct tatttaaata atgaaaaatc atatttcgtt ttagtagatc cgaattcttt taaaactgaa atagccctac aagagctggt aattttacct acgaataaaa tagaaaaaga aaatcttcta aaaaaattaa gtaaaactcc gtatattaaa gttttaaata aatatagctc tacaccttat atacaacaaa attacggtgc tacgagtagc atgtataaag taaaaggaca gttcttgaca attgacatgt gtccttcttc caaaagcttt gaaaaagatt tttttaaaaa gctcgtagaa ttatcgatca aactaaataa acctattccg attgctatat gtgtttcagg attatggatt aataagcata cagaagaatt cttatggtta ttaaaacaac aagaaaacgg ttatttacaa ataacatggg ttaatcattc atttagtcat ccttatttta aagataaacc tcttgaagat aatttccttc tttccaataa agatgatttt gaaaatgaag tattagaagc agggaaaata ttagtaagtt ataatattgc tccgtcacct ttctttcgtt ttcctggatt agtttccgat caaacattaa tagaaaaatt aaaagatttg ggatttattc cgcttgggag taatgcatgg cttgctaaag gcgaaaaagt tcaaaatggt tcttttatat tggtacacgg taatagtaac gaaaaagccg gaatagattt aattatgccg atgttaccgg aattaaaatt acttcctatt gaaaaggcgt ttttattaca tgacaattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for RicoA.17612.a.B1.PW35930
PURIFICATION DATe: 8/20/2012
CONCENTRATION: 24.1mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML FFSLSYASEK AVIITDYKPV FLPVIAENKK IRIAIRSYLN NEKSYFVLVD PNSFKTEIAL QELVILPTNK IEKENLLKKL SKTPYIKVLN KYSSTPYIQQ NYGATSSMYK VKGQFLTIDM CPSSKSFEKD FFKKLVELSI KLNKPIPIAI CVSGLWINKH TEEFLWLLKQ QENGYLQITW VNHSFSHPYF KDKPLEDNFL LSNKDDFENE VLEAGKILVS YNIAPSPFFR FPGLVSDQTL IEKLKDLGFI PLGSNAWLAK GEKVQNGSFI LVHGNSNEKA GIDLIMPMLP ELKLLPIEKA FLLHDN
Validated NT Sequence
tcttactcac ttaattgtca tgtaataaaa acgccttttc aataggaagt aattttaatt ccggtaacat cggcataatt aaatctattc cggctttttc gttactatta ccgtgtacca atataaaaga accattttga actttttcgc ctttagcaag ccatgcatta ctcccaagcg gaataaatcc caaatctttt aatttttcta ttaatgtttg atcggaaact aatccaggaa aacgaaagaa aggtgacgga gcaatattat aacttactaa tattttccct gcttctaata cttcattttc aaaatcatct ttattggaaa gaaggaaatt atcttcaaga ggtttatctt taaaataagg atgactaaat gaatgattaa cccatgttat ttgtaaataa ccgttttctt gttgttttaa taaccataag aattcttctg tatgcttatt aatccataat cctgaaacac atatagcaat cggaataggt ttatttagtt tgatcgataa ttctacgagc tttttaaaaa aatctttttc aaagcttttg gaagaaggac acatgtcaat tgtcaagaac tgtcctttta ctttatacat gctactcgta gcaccgtaat tttgttgtat ataaggtgta gagctatatt tatttaaaac tttaatatac ggagttttac ttaatttttt tagaagattt tctttttcta ttttattcgt aggtaaaatt accagctctt gtagggctat ttcagtttta aaagaattcg gatctactaa aacgaaatat gatttttcat tatttaaata agaacgaata gcaattctta tttttttatt ttcagcaatt acaggcaaaa ataccggttt ataatcagtt ataattactg ctttttcact tgcatagctt aaactaaaaa acaacatatg gtggtggtgg tggtgagcca tggta
Expressed Protein Sequence
MAHHHHHHML FFSLSYASEK AVIITDYKPV FLPVIAENKK IRIAIRSYLN NEKSYFVLVD PNSFKTEIAL QELVILPTNK IEKENLLKKL SKTPYIKVLN KYSSTPYIQQ NYGATSSMYK VKGQFLTIDM CPSSKSFEKD FFKKLVELSI KLNKPIPIAI CVSGLWINKH TEEFLWLLKQ QENGYLQITW VNHSFSHPYF KDKPLEDNFL LSNKDDFENE VLEAGKILVS YNIAPSPFFR FPGLVSDQTL IEKLKDLGFI PLGSNAWLAK GEKVQNGSFI LVHGNSNEKA GIDLIMPMLP ELKLLPIEKA FLLHDN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGT TTTTTAGTTT AAGCTATGCA AGTGAAAAAG CAGTAATTAT AACTGATTAT AAACCGGTAT TTTTGCCTGT AATTGCTGAA AATAAAAAAA TAAGAATTGC TATTCGTTCT TATTTAAATA ATGAAAAATC ATATTTCGTT TTAGTAGATC CGAATTCTTT TAAAACTGAA ATAGCCCTAC AAGAGCTGGT AATTTTACCT ACGAATAAAA TAGAAAAAGA AAATCTTCTA AAAAAATTAA GTAAAACTCC GTATATTAAA GTTTTAAATA AATATAGCTC TACACCTTAT ATACAACAAA ATTACGGTGC TACGAGTAGC ATGTATAAAG TAAAAGGACA GTTCTTGACA ATTGACATGT GTCCTTCTTC CAAAAGCTTT GAAAAAGATT TTTTTAAAAA GCTCGTAGAA TTATCGATCA AACTAAATAA ACCTATTCCG ATTGCTATAT GTGTTTCAGG ATTATGGATT AATAAGCATA CAGAAGAATT CTTATGGTTA TTAAAACAAC AAGAAAACGG TTATTTACAA ATAACATGGG TTAATCATTC ATTTAGTCAT CCTTATTTTA AAGATAAACC TCTTGAAGAT AATTTCCTTC TTTCCAATAA AGATGATTTT GAAAATGAAG TATTAGAAGC AGGGAAAATA TTAGTAAGTT ATAATATTGC TCCGTCACCT TTCTTTCGTT TTCCTGGATT AGTTTCCGAT CAAACATTAA TAGAAAAATT AAAAGATTTG GGATTTATTC CGCTTGGGAG TAATGCATGG CTTGCTAAAG GCGAAAAAGT TCAAAATGGT TCTTTTATAT TGGTACACGG TAATAGTAAC GAAAAAGCCG GAATAGATTT AATTATGCCG ATGTTACCGG AATTAAAATT ACTTCCTATT GAAAAGGCGT TTTTATTACA TGACAATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for RicoA.17612.a.B1.PW36740
PURIFICATION DATe: 8/20/2012
CONCENTRATION: 6.28mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML FFSLSYASEK AVIITDYKPV FLPVIAENKK IRIAIRSYLN NEKSYFVLVD PNSFKTEIAL QELVILPTNK IEKENLLKKL SKTPYIKVLN KYSSTPYIQQ NYGATSSMYK VKGQFLTIDM CPSSKSFEKD FFKKLVELSI KLNKPIPIAI CVSGLWINKH TEEFLWLLKQ QENGYLQITW VNHSFSHPYF KDKPLEDNFL LSNKDDFENE VLEAGKILVS YNIAPSPFFR FPGLVSDQTL IEKLKDLGFI PLGSNAWLAK GEKVQNGSFI LVHGNSNEKA GIDLIMPMLP ELKLLPIEKA FLLHDN
Validated NT Sequence
tcttactcac ttaattgtca tgtaataaaa acgccttttc aataggaagt aattttaatt ccggtaacat cggcataatt aaatctattc cggctttttc gttactatta ccgtgtacca atataaaaga accattttga actttttcgc ctttagcaag ccatgcatta ctcccaagcg gaataaatcc caaatctttt aatttttcta ttaatgtttg atcggaaact aatccaggaa aacgaaagaa aggtgacgga gcaatattat aacttactaa tattttccct gcttctaata cttcattttc aaaatcatct ttattggaaa gaaggaaatt atcttcaaga ggtttatctt taaaataagg atgactaaat gaatgattaa cccatgttat ttgtaaataa ccgttttctt gttgttttaa taaccataag aattcttctg tatgcttatt aatccataat cctgaaacac atatagcaat cggaataggt ttatttagtt tgatcgataa ttctacgagc tttttaaaaa aatctttttc aaagcttttg gaagaaggac acatgtcaat tgtcaagaac tgtcctttta ctttatacat gctactcgta gcaccgtaat tttgttgtat ataaggtgta gagctatatt tatttaaaac tttaatatac ggagttttac ttaatttttt tagaagattt tctttttcta ttttattcgt aggtaaaatt accagctctt gtagggctat ttcagtttta aaagaattcg gatctactaa aacgaaatat gatttttcat tatttaaata agaacgaata gcaattctta tttttttatt ttcagcaatt acaggcaaaa ataccggttt ataatcagtt ataattactg ctttttcact tgcatagctt aaactaaaaa acaacatatg gtggtggtgg tggtgagcca tggta
Expressed Protein Sequence
MAHHHHHHML FFSLSYASEK AVIITDYKPV FLPVIAENKK IRIAIRSYLN NEKSYFVLVD PNSFKTEIAL QELVILPTNK IEKENLLKKL SKTPYIKVLN KYSSTPYIQQ NYGATSSMYK VKGQFLTIDM CPSSKSFEKD FFKKLVELSI KLNKPIPIAI CVSGLWINKH TEEFLWLLKQ QENGYLQITW VNHSFSHPYF KDKPLEDNFL LSNKDDFENE VLEAGKILVS YNIAPSPFFR FPGLVSDQTL IEKLKDLGFI PLGSNAWLAK GEKVQNGSFI LVHGNSNEKA GIDLIMPMLP ELKLLPIEKA FLLHDN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGT TTTTTAGTTT AAGCTATGCA AGTGAAAAAG CAGTAATTAT AACTGATTAT AAACCGGTAT TTTTGCCTGT AATTGCTGAA AATAAAAAAA TAAGAATTGC TATTCGTTCT TATTTAAATA ATGAAAAATC ATATTTCGTT TTAGTAGATC CGAATTCTTT TAAAACTGAA ATAGCCCTAC AAGAGCTGGT AATTTTACCT ACGAATAAAA TAGAAAAAGA AAATCTTCTA AAAAAATTAA GTAAAACTCC GTATATTAAA GTTTTAAATA AATATAGCTC TACACCTTAT ATACAACAAA ATTACGGTGC TACGAGTAGC ATGTATAAAG TAAAAGGACA GTTCTTGACA ATTGACATGT GTCCTTCTTC CAAAAGCTTT GAAAAAGATT TTTTTAAAAA GCTCGTAGAA TTATCGATCA AACTAAATAA ACCTATTCCG ATTGCTATAT GTGTTTCAGG ATTATGGATT AATAAGCATA CAGAAGAATT CTTATGGTTA TTAAAACAAC AAGAAAACGG TTATTTACAA ATAACATGGG TTAATCATTC ATTTAGTCAT CCTTATTTTA AAGATAAACC TCTTGAAGAT AATTTCCTTC TTTCCAATAA AGATGATTTT GAAAATGAAG TATTAGAAGC AGGGAAAATA TTAGTAAGTT ATAATATTGC TCCGTCACCT TTCTTTCGTT TTCCTGGATT AGTTTCCGAT CAAACATTAA TAGAAAAATT AAAAGATTTG GGATTTATTC CGCTTGGGAG TAATGCATGG CTTGCTAAAG GCGAAAAAGT TCAAAATGGT TCTTTTATAT TGGTACACGG TAATAGTAAC GAAAAAGCCG GAATAGATTT AATTATGCCG ATGTTACCGG AATTAAAATT ACTTCCTATT GAAAAGGCGT TTTTATTACA TGACAATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT