RicoA.17484.a

Protein mrp homolog

CENTER ID: RicoA.17484.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.17484.a.B1.GE35295 Full length( RicoA.17484.a ) 1 319
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.192
RefSeq: NP_359800.1
UniProt: Q92JA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANLHQRQII DKIQHITFKD GTFLNEVISD IIIKGNNIGF SIDISGKNKL EAEEIRLKAI NKLNEISEVN KITIVFTESK PMEKKVQKPK HFVENVKKII LVASGKGGVG KSTISALIAQ QLSLANYRVG IVDADIYGPS IPHIFGINEV PQTKDGRIIP VLAQSIEIIS IGFFVKDHSA IIWRGPMASK TIYQLLSVTK WDNLDYLIID MPPGTGDIHL SILENYHLDG VIIVTTPQKI SEIDVIRSID LYQKLNLPIL GIIENMSYML KNNSGGHLSQ KYNIPLIAQM PITPQIAEAC DKSLPLTNLL TLPLEKYLQ
NT Sequence
ATGGCGAATT TACACCAACG ACAAATTATA GATAAAATTC AGCATATTAC CTTTAAAGAC GGTACTTTTT TAAATGAAGT TATATCGGAT ATTATAATTA AAGGTAATAA TATCGGTTTC TCAATAGATA TATCAGGTAA AAACAAGTTA GAAGCCGAAG AAATTAGACT TAAGGCAATT AATAAACTTA ACGAGATTTC GGAAGTAAAT AAAATAACAA TTGTTTTTAC CGAAAGTAAA CCGATGGAGA AAAAAGTTCA AAAACCAAAA CATTTTGTAG AAAATGTAAA AAAAATTATC TTAGTAGCGT CAGGTAAAGG CGGAGTCGGA AAATCTACAA TATCGGCTCT TATCGCTCAG CAGTTAAGTC TAGCAAATTA CCGAGTCGGG ATAGTAGATG CGGATATTTA CGGTCCGTCA ATTCCGCATA TATTCGGCAT TAACGAAGTA CCGCAAACGA AAGATGGACG AATAATACCG GTACTTGCTC AAAGCATCGA AATTATATCT ATAGGCTTCT TCGTTAAAGA CCACTCGGCA ATTATTTGGC GTGGTCCTAT GGCGAGTAAG ACTATTTATC AATTATTATC GGTAACAAAA TGGGATAATT TAGATTATTT AATTATCGAT ATGCCGCCGG GAACAGGTGA TATTCATTTA AGTATACTAG AAAATTATCA TTTAGACGGC GTAATAATCG TAACTACACC ACAAAAAATA TCAGAAATAG ACGTAATACG TTCTATAGAT TTATATCAAA AACTAAATTT ACCTATTTTG GGAATAATTG AGAATATGAG CTATATGCTT AAAAATAATA GTGGCGGACA TTTATCACAA AAATATAATA TACCACTAAT CGCTCAAATG CCGATCACAC CACAAATAGC CGAGGCTTGC GATAAATCAC TACCTCTAAC AAACTTATTA ACATTACCTT TAGAGAAGTA TTTACAA
Details for RicoA.17484.a.B1.GE35295
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 72
Validated AA Sequence
MEKKVQKPKH FVENVKKIIL VASGKGGVGK STISALIAQQ LSLANYRVGI VDADIYGPSI PHIFGINEVP QTKDGRIIPV LAQSIEIISI GFFVKDHSAI IWRGPMASKT IYQLLSVTKW DNLDYLIIDM PPGTGDIHLS ILENYHLDGV IIVTTPQKIS EIDVIRSIDL YQKLNLPILG IIENMSYMLK NNSGGHLSQK YNIPLIAQMP ITPQIAEACD KSLPLTNLLT LPLEKYLQ
Validated NT Sequence
tcttactcac ttattgtaaa tacttctcta aaggtaatgt taataagttt gttagaggta gtgatttatc gcaagcctcg gctatttgtg gtgtgatcgg catttgagcg attagtggta tattatattt ttgtgataaa tgtccgccac tattattttt aagcatatag ctcatattct caattattcc caaaataggt aaatttagtt tttgatataa atctatagaa cgtattacgt ctatttctga tattttttgt ggtgtagtta cgattattac gccgtctaaa tgataatttt ctagtatact taaatgaata tcacctgttc ccggcggcat atcgataatt aaataatcta aattatccca ttttgttacc gataataatt gataaatagt cttactcgcc ataggaccac gccaaataat tgccgagtgg tctttaacga agaagcctat agatataatt tcgatgcttt gagcaagtac cggtattatt cgtccatctt tcgtttgcgg tacttcgtta atgccgaata tatgcggaat tgacggaccg taaatatccg catctactat cccgactcgg taatttgcta gacttaactg ctgagcgata agagccgata ttgtagattt tccgactccg cctttacctg acgctactaa gataattttt tttacatttt ctacaaaatg ttttggtttt tgaacttttt tctccatcgg tttactttcg gtaaaaacaa ttgttatttt atttacttcc gaaatctcgt taagtttatt aattgcctta agtctaattt cttcggcttc taacttgttt ttacctgata tatctattga gaaaccgata ttattacctt taattataat atccgatata acttcattta aaaaagtacc gtctttaaag gtaatatgct gaattttatc tataatttgt cgttggtgta a
Expected Protein Sequence
MAHHHHHHMA NLHQRQIIDK IQHITFKDGT FLNEVISDII IKGNNIGFSI DISGKNKLEA EEIRLKAINK LNEISEVNKI TIVFTESKPM EKKVQKPKHF VENVKKIILV ASGKGGVGKS TISALIAQQL SLANYRVGIV DADIYGPSIP HIFGINEVPQ TKDGRIIPVL AQSIEIISIG FFVKDHSAII WRGPMASKTI YQLLSVTKWD NLDYLIIDMP PGTGDIHLSI LENYHLDGVI IVTTPQKISE IDVIRSIDLY QKLNLPILGI IENMSYMLKN NSGGHLSQKY NIPLIAQMPI TPQIAEACDK SLPLTNLLTL PLEKYLQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga atttacacca acgacaaatt atagataaaa ttcagcatat tacctttaaa gacggtactt ttttaaatga agttatatcg gatattataa ttaaaggtaa taatatcggt ttctcaatag atatatcagg taaaaacaag ttagaagccg aagaaattag acttaaggca attaataaac ttaacgagat ttcggaagta aataaaataa caattgtttt taccgaaagt aaaccgatgg agaaaaaagt tcaaaaacca aaacattttg tagaaaatgt aaaaaaaatt atcttagtag cgtcaggtaa aggcggagtc ggaaaatcta caatatcggc tcttatcgct cagcagttaa gtctagcaaa ttaccgagtc gggatagtag atgcggatat ttacggtccg tcaattccgc atatattcgg cattaacgaa gtaccgcaaa cgaaagatgg acgaataata ccggtacttg ctcaaagcat cgaaattata tctataggct tcttcgttaa agaccactcg gcaattattt ggcgtggtcc tatggcgagt aagactattt atcaattatt atcggtaaca aaatgggata atttagatta tttaattatc gatatgccgc cgggaacagg tgatattcat ttaagtatac tagaaaatta tcatttagac ggcgtaataa tcgtaactac accacaaaaa atatcagaaa tagacgtaat acgttctata gatttatatc aaaaactaaa tttacctatt ttgggaataa ttgagaatat gagctatatg cttaaaaata atagtggcgg acatttatca caaaaatata atataccact aatcgctcaa atgccgatca caccacaaat agccgaggct tgcgataaat cactacctct aacaaactta ttaacattac ctttagagaa gtatttacaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat