RicoA.17313.a

Probable oxygen-independent coproporphyrinogen III oxidase

CENTER ID: RicoA.17313.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.17313.a.B1.GE35329 Full length( RicoA.17313.a ) 1 400
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.252
RefSeq: NP_359856.1
UniProt: Q92J50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTKPKLALA KIQNMRVMAN DLSIYIHWPF CVSKCPYCDF NSHVASTIDH NQWLKSYETE IEYFKDIIQN KYIKSIFFGG GTPSLMNPVI VEGIINKIST LAIIDNQTEI TLETNPTSFE TEKFKAFKAV GINRISIGVQ SLKEDDLKKL GRTHDCMQAI KTIEAANTIF LRVSFDLIYA RSGQTLKDWQ EELKQAMQLA TSHISLYQLT IEKGTPCYKL FKEGNLILPH SDAAAEMYEW TNHYLESKKY FRYEISNYAM IGYECLHNLT YWNYNSYLGI GPGAHSRIIE SSSSVSAIMM WHKPEKWLDA VKTKNVGIQT NTKLTHQEII EEMLMMGLRL SKGINISTLE QKLNTKLENI LDMNNLKHYQ ALDLIRLDEN IYLTDKGLML YSYIVPRLII
NT Sequence
ATGTCCACAA AACCTAAGTT AGCACTAGCA AAAATACAAA ATATGAGAGT TATGGCTAAT GATTTAAGTA TTTATATTCA TTGGCCTTTT TGTGTGTCCA AATGTCCGTA TTGTGATTTT AATTCTCATG TAGCAAGTAC TATAGATCAT AATCAGTGGC TTAAATCTTA TGAAACTGAA ATTGAATATT TTAAGGATAT TATTCAAAAT AAATATATAA AATCTATTTT TTTTGGGGGC GGTACTCCCT CTTTAATGAA TCCTGTAATA GTTGAAGGCA TAATAAATAA AATCAGTACT CTAGCAATTA TTGATAATCA AACCGAGATA ACTCTAGAAA CTAATCCCAC ATCATTTGAA ACTGAAAAAT TTAAAGCATT CAAAGCCGTC GGAATTAATC GTATTTCAAT AGGAGTGCAA TCCTTAAAAG AGGACGATTT AAAAAAATTA GGTAGAACTC ATGATTGTAT GCAGGCCATT AAAACAATTG AGGCGGCAAA TACGATTTTT CTACGAGTGT CATTTGATTT AATATATGCA CGTAGCGGTC AGACATTAAA AGACTGGCAA GAAGAATTAA AACAAGCTAT GCAGCTCGCT ACCTCACATA TTTCTCTTTA TCAATTGACT ATCGAAAAAG GTACGCCGTG TTATAAATTA TTTAAGGAGG GTAATCTGAT TTTGCCGCAT TCAGATGCGG CAGCTGAAAT GTATGAATGG ACTAATCATT ATCTAGAATC TAAAAAGTAT TTTAGATATG AAATATCCAA TTATGCTATG ATAGGGTATG AATGTTTGCA TAATTTAACT TATTGGAATT ATAATAGCTA TTTAGGTATA GGCCCCGGAG CTCATAGTAG AATCATTGAA TCCTCAAGTT CGGTATCGGC AATTATGATG TGGCATAAAC CTGAAAAATG GTTAGATGCG GTTAAAACAA AAAATGTTGG TATTCAAACT AATACTAAGT TAACACATCA AGAGATTATT GAAGAAATGC TAATGATGGG TTTACGCCTT AGCAAAGGTA TAAATATTTC TACATTAGAG CAGAAATTAA ACACGAAATT AGAGAATATT TTAGATATGA ATAATCTTAA GCATTATCAA GCGTTAGACT TAATTAGACT AGATGAAAAT ATCTATCTTA CCGACAAAGG TTTAATGCTG TATAGCTACA TAGTACCTAG ATTGATTATA
Details for RicoA.17313.a.B1.GE35329
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MRVMANDLSI YIHWPFCVSK CPYCDFNSHV ASTIDHNQWL KSYETEIEYF KDIIQNKYIK SIFFGGGTPS LMNPVIVEGI INKISTLAII DNQTEITLET NPTSFETEKF KAFKAVGINR ISIGVQSLKE DDLKKLGRTH DCMQAIKTIE AANTIFLRVS FDLIYARSGQ TLKDWQEELK QAMQLATSHI SLYQLTIEKG TPCYKLFKEG NLILPHSDAA AEMYEWTNHY LESKKYFRYE ISNYAMIGYE CLHNLTYWNY NSYLGIGPGA HSRIIESSSS VSAIMMWHKP EKWLDAVKTK NVGIQTNTKL THQEIIEEML MMGLRLSKGI NISTLEQKLN TKLENILDMN NLKHYQALDL IRLDENIYLT DKGLML
Validated NT Sequence
atacagcatt aaacctttgt cggtaagata gatattttca tctagtctaa ttaagtctaa cgcttgataa tgcttaagat tattcatatc taaaatattc tctaatttcg tgtttaattt ctgctctaat gtagaaatat ttataccttt gctaaggcgt aaacccatca ttagcatttc ttcaataatc tcttgatgtg ttaacttagt attagtttga ataccaacat tttttgtttt aaccgcatct aaccattttt caggtttatg ccacatcata attgccgata ccgaacttga ggattcaatg attctactat gagctccggg gcctatacct aaatagctat tataattcca ataagttaaa ttatgcaaac attcataccc tatcatagca taattggata tttcatatct aaaatacttt ttagattcta gataatgatt agtccattca tacatttcag ctgccgcatc tgaatgcggc aaaatcagat taccctcctt aaataattta taacacggcg tacctttttc gatagtcaat tgataaagag aaatatgtga ggtagcgagc tgcatagctt gttttaattc ttcttgccag tcttttaatg tctgaccgct acgtgcatat attaaatcaa atgacactcg tagaaaaatc gtatttgccg cctcaattgt tttaatggcc tgcatacaat catgagttct acctaatttt tttaaatcgt cctcttttaa ggattgcact cctattgaaa tacgattaat tccgacggct ttgaatgctt taaatttttc agtttcaaat gatgtgggat tagtttctag agttatctcg gtttgattat caataattgc tagagtactg attttattta ttatgccttc aactattaca ggattcatta aagagggagt accgccccca aaaaaaatag attttatata tttattttga ataatatcct taaaatattc aatttcagtt tcataagatt taagccactg attatgatct atagtacttg ctacatgaga attaaaatca caatacggac atttggacac acaaaaaggc caatgaatat aaatacttaa atcattagcc ataactctca tattttgtat ttttgctagt gctaacttag gttt
Expected Protein Sequence
MAHHHHHHMS TKPKLALAKI QNMRVMANDL SIYIHWPFCV SKCPYCDFNS HVASTIDHNQ WLKSYETEIE YFKDIIQNKY IKSIFFGGGT PSLMNPVIVE GIINKISTLA IIDNQTEITL ETNPTSFETE KFKAFKAVGI NRISIGVQSL KEDDLKKLGR THDCMQAIKT IEAANTIFLR VSFDLIYARS GQTLKDWQEE LKQAMQLATS HISLYQLTIE KGTPCYKLFK EGNLILPHSD AAAEMYEWTN HYLESKKYFR YEISNYAMIG YECLHNLTYW NYNSYLGIGP GAHSRIIESS SSVSAIMMWH KPEKWLDAVK TKNVGIQTNT KLTHQEIIEE MLMMGLRLSK GINISTLEQK LNTKLENILD MNNLKHYQAL DLIRLDENIY LTDKGLMLYS YIVPRLII
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca caaaacctaa gttagcacta gcaaaaatac aaaatatgag agttatggct aatgatttaa gtatttatat tcattggcct ttttgtgtgt ccaaatgtcc gtattgtgat tttaattctc atgtagcaag tactatagat cataatcagt ggcttaaatc ttatgaaact gaaattgaat attttaagga tattattcaa aataaatata taaaatctat tttttttggg ggcggtactc cctctttaat gaatcctgta atagttgaag gcataataaa taaaatcagt actctagcaa ttattgataa tcaaaccgag ataactctag aaactaatcc cacatcattt gaaactgaaa aatttaaagc attcaaagcc gtcggaatta atcgtatttc aataggagtg caatccttaa aagaggacga tttaaaaaaa ttaggtagaa ctcatgattg tatgcaggcc attaaaacaa ttgaggcggc aaatacgatt tttctacgag tgtcatttga tttaatatat gcacgtagcg gtcagacatt aaaagactgg caagaagaat taaaacaagc tatgcagctc gctacctcac atatttctct ttatcaattg actatcgaaa aaggtacgcc gtgttataaa ttatttaagg agggtaatct gattttgccg cattcagatg cggcagctga aatgtatgaa tggactaatc attatctaga atctaaaaag tattttagat atgaaatatc caattatgct atgatagggt atgaatgttt gcataattta acttattgga attataatag ctatttaggt ataggccccg gagctcatag tagaatcatt gaatcctcaa gttcggtatc ggcaattatg atgtggcata aacctgaaaa atggttagat gcggttaaaa caaaaaatgt tggtattcaa actaatacta agttaacaca tcaagagatt attgaagaaa tgctaatgat gggtttacgc cttagcaaag gtataaatat ttctacatta gagcagaaat taaacacgaa attagagaat attttagata tgaataatct taagcattat caagcgttag acttaattag actagatgaa aatatctatc ttaccgacaa aggtttaatg ctgtatagct acatagtacc tagattgatt atatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat