SSGCID: Target Page

RicoA.17300.a

Outer membrane protein tolC

CENTER ID:	RicoA.17300.a
ORGANISM:	Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE:	Boutonneuse fever
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RicoA.17300.a.B2.GE36557	Mature protein( RicoA.17300.a )		19	453

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272944.4.peg.351
RefSeq:	NP_359943.1
UniProt:	Q92IW4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRKLITFIVT LLLTSSVAAV DLQGALTEGY KNNEELKAAR IKFLDSMEQF PRAFSGFMPS AGLQINRQHN KTKYNKKYAD RLGLTPRDTD SDQGALTIQQ SLFNGGSSVA ALKAAQAGFR AARGAYYAGE QKILLNLITA YLDCFESKAK YDISESRVRT NIQQVNTVEE KLRLGEATEV DIATARAGLA AAETNKLAAY ADFQGKKANF IRVFGIEPTN ITMPELPKML PASLDELTRR AAKLNPDIDS ARHNVTAAKA LEMAEKGKLL PQVSVQLQSG RTYYNPQGDI NAINNRSYTT TLSVNIPIYP EGGAQYSRIR SAKNQTRNSA VQLDNVIKQT QAGVVSVWEG FEAAKSRIVA ANQGVDAAQI SYDGTVQEEI VGSKTILDVL TAEEKLYEAK ITRVDAYKSS VLAAYQMKLL TGELTAQSLK LKVKYFSPEE EFKTIKKKMF IGF

NT Sequence

ATGCGTAAAT TAATTACATT TATTGTTACA TTATTACTAA CTAGTTCGGT TGCAGCAGTT GATTTGCAGG GAGCTTTAAC TGAAGGATAT AAGAATAATG AGGAGCTGAA AGCTGCTAGA ATTAAGTTTT TAGACTCGAT GGAGCAGTTT CCGCGAGCTT TTTCAGGGTT TATGCCTAGT GCCGGATTAC AGATTAATAG ACAACATAAT AAGACTAAAT ATAATAAAAA ATATGCTGAT AGGCTTGGTC TTACTCCAAG GGACACAGAT AGTGATCAAG GTGCATTAAC AATTCAACAG TCTCTATTTA ATGGTGGTTC TAGCGTTGCG GCTCTTAAAG CTGCTCAAGC AGGATTTAGA GCTGCACGGG GTGCATATTA TGCCGGTGAA CAAAAAATAT TATTAAATTT AATAACTGCG TATCTTGATT GTTTTGAGAG CAAAGCGAAA TATGATATTT CCGAAAGTAG AGTTCGCACT AACATACAAC AAGTTAACAC CGTTGAGGAA AAATTAAGAC TTGGAGAAGC AACGGAAGTA GATATAGCAA CCGCAAGAGC GGGGCTTGCA GCAGCAGAAA CGAATAAACT AGCTGCTTAT GCCGATTTTC AAGGAAAAAA AGCAAATTTT ATTAGAGTAT TTGGAATAGA GCCGACTAAT ATAACTATGC CTGAGTTACC TAAAATGTTA CCTGCTTCAT TAGATGAGTT AACTAGAAGA GCTGCTAAAC TCAATCCTGA TATTGATTCG GCAAGGCATA ATGTAACTGC GGCTAAAGCT TTAGAAATGG CAGAAAAAGG GAAATTATTA CCGCAAGTAA GTGTGCAACT GCAATCTGGT AGAACATACT ACAATCCACA AGGTGATATA AATGCTATAA ATAATAGAAG CTATACTACT ACTCTTTCGG TAAATATCCC TATTTATCCT GAGGGCGGAG CTCAATATTC AAGAATCAGA TCGGCTAAAA ATCAAACAAG AAATAGTGCA GTTCAGCTTG ATAATGTGAT AAAGCAGACC CAAGCAGGGG TTGTAAGCGT GTGGGAGGGA TTTGAAGCAG CAAAATCTCG TATTGTTGCC GCTAATCAAG GAGTGGATGC TGCTCAAATA TCATATGACG GTACTGTGCA AGAGGAAATA GTCGGATCTA AAACAATATT AGATGTTTTA ACTGCCGAAG AAAAATTATA CGAAGCAAAA ATAACTCGTG TGGATGCGTA TAAATCTTCA GTACTTGCGG CATATCAAAT GAAATTGTTA ACCGGCGAGC TAACGGCTCA AAGTTTAAAA CTTAAAGTAA AATATTTTAG TCCTGAAGAA GAGTTTAAGA CTATTAAAAA GAAAATGTTT ATAGGTTTC

Details for RicoA.17300.a.B2.GE36557

HARVESTED ON:	1/9/2013
SEQUENCED ON:	1/8/2013
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA VDLQGALTEG YKNNEELKAA RIKFLDSMEQ FPRAFSGFMP SAGLQINRQH NKTKYNKKYA DRLGLTPRDT DSDQGALTIQ QSLFNGGSSV AALKAAQAGF RAARGAYYAG EQKILLNLIT AYLDCFESKA KYDISESRVR TNIQQVNTVE EKLRLGEATE VDIATARAGL AAAETNKLAA YADFQGKKAN FIRVFGIEPT NITMPELPKM LPASLDELTR RAAKLNPDID SARHNVTAAK ALEMAEKGKL LPQVSVQLQS GRTYYNPQGD INAINNRSYT TTLSVNIPIY PEGGAQYSRI RSAKNQTRNS AVQLDNVIKQ TQAGVVSVWE GFEAAKSRIV AANQGVDAAQ ISYDGTVQEE IVGSKTILDV LTAEEKLYEA KITRVDAYKS SVLAAYQMKL LTGELTAQSL KLKVKYFSPE EEFKT

Validated NT Sequence

atggctcacc accaccacca ccatatggca gttgatttgc agggagcttt aactgaagga tataagaata atgaggagct gaaagctgct agaattaagt ttttagactc gatggagcag tttccgcgag ctttttcagg gtttatgcct agtgccggat tacagattaa tagacaacat aataagacta aatataataa aaaatatgct gataggcttg gtcttactcc aagggacaca gatagtgatc aaggtgcatt aacaattcaa cagtctctat ttaatggtgg ttctagcgtt gcggctctta aagctgctca agcaggattt agagctgcac ggggtgcata ttatgccggt gaacaaaaaa tattattaaa tttaataact gcgtatcttg attgttttga gagcaaagcg aaatatgata tttccgaaag tagagttcgc actaacatac aacaagttaa caccgttgag gaaaaattaa gacttggaga agcaacggaa gtagatatag caaccgcaag agcggggctt gcagcagcag aaacgaataa actagctgct tatgccgatt ttcaaggaaa aaaagcaaat tttattagag tatttggaat agagccgact aatataacta tgcctgagtt acctaaaatg ttacctgctt cattagatga gttaactaga agagctgcta aactcaatcc tgatattgat tcggcaaggc ataatgtaac tgcggctaaa gctttagaaa tggcagaaaa agggaaatta ttaccgcaag taagtgtgca actgcaatct ggtagaacat actacaatcc acaaggtgat ataaatgcta taaataatag aagctatact actactcttt cggtaaatat ccctatttat cctgagggcg gagctcaata ttcaagaatc agatcggcta aaaatcaaac aagaaatagt gcagttcagc ttgataatgt gataaagcag acccaagcag gggttgtaag cgtgtgggag ggatttgaag cagcaaaatc tcgtattgtt gccgctaatc aaggagtgga tgctgctcaa atatcatatg acggtactgt gcaagaggaa atagtcggat ctaaaacaat attagatgtt ttaactgccg aagaaaaatt atacgaagca aaaataactc gtgtggatgc gtataaatct tcagtacttg cggcatatca aatgaaattg ttaaccggcg agctaacggc tcaaagttta aaacttaaag taaaatattt tagtcctgaa gaagagttta agactat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag ttgatttgca gggagcttta actgaaggat ataagaataa tgaggagctg aaagctgcta gaattaagtt tttagactcg atggagcagt ttccgcgagc tttttcaggg tttatgccta gtgccggatt acagattaat agacaacata ataagactaa atataataaa aaatatgctg ataggcttgg tcttactcca agggacacag atagtgatca aggtgcatta acaattcaac agtctctatt taatggtggt tctagcgttg cggctcttaa agctgctcaa gcaggattta gagctgcacg gggtgcatat tatgccggtg aacaaaaaat attattaaat ttaataactg cgtatcttga ttgttttgag agcaaagcga aatatgatat ttccgaaagt agagttcgca ctaacataca acaagttaac accgttgagg aaaaattaag acttggagaa gcaacggaag tagatatagc aaccgcaaga gcggggcttg cagcagcaga aacgaataaa ctagctgctt atgccgattt tcaaggaaaa aaagcaaatt ttattagagt atttggaata gagccgacta atataactat gcctgagtta cctaaaatgt tacctgcttc attagatgag ttaactagaa gagctgctaa actcaatcct gatattgatt cggcaaggca taatgtaact gcggctaaag ctttagaaat ggcagaaaaa gggaaattat taccgcaagt aagtgtgcaa ctgcaatctg gtagaacata ctacaatcca caaggtgata taaatgctat aaataataga agctatacta ctactctttc ggtaaatatc cctatttatc ctgagggcgg agctcaatat tcaagaatca gatcggctaa aaatcaaaca agaaatagtg cagttcagct tgataatgtg ataaagcaga cccaagcagg ggttgtaagc gtgtgggagg gatttgaagc agcaaaatct cgtattgttg ccgctaatca aggagtggat gctgctcaaa tatcatatga cggtactgtg caagaggaaa tagtcggatc taaaacaata ttagatgttt taactgccga agaaaaatta tacgaagcaa aaataactcg tgtggatgcg tataaatctt cagtacttgc ggcatatcaa atgaaattgt taaccggcga gctaacggct caaagtttaa aacttaaagt aaaatatttt agtcctgaag aagagtttaa gactattaaa aagaaaatgt ttataggttt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat