RicoA.10014.e

Putative ABC transporter ATP-binding protein

CENTER ID: RicoA.10014.e
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.10014.e.B1.GE35292 Full length( RicoA.10014.e ) 1 240
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.10014.e.B1.PW35910 Full length( RicoA.10014.e ) 1 240

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.571
RefSeq: NP_360137.1
UniProt: Q92IC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKPYLYAENI QFKVKERTEP IIAETTLNIA KEEFVVILGH NGSGKSTLAK ILAGYLKPTS GKVFLDQVRI DKIPKGQKAS TLVTLTQKAE ERLFTELTLE ENIILWESRF SSNERLASSE LLELTGAPKR FLPLLSQPLG KFSGGEKQMI LLALIIAHPP KILFLDEHTA NLDPKASLEV MKKTAEIIEK HKITSVMITH NLEDAVNYGK RLIVLDSGKV VLDYLKPPGF SLKELKEILN
NT Sequence
ATGAAACCTT ATTTATATGC AGAAAATATA CAGTTCAAAG TTAAAGAAAG AACCGAACCT ATAATTGCTG AAACTACTTT AAATATTGCT AAAGAAGAGT TTGTGGTAAT ATTGGGTCAT AACGGTAGCG GTAAGTCTAC TTTAGCTAAA ATACTTGCCG GCTATTTAAA ACCTACTAGC GGGAAAGTGT TTCTAGATCA AGTCAGAATC GATAAAATAC CTAAAGGACA AAAAGCTTCT ACATTAGTTA CATTAACACA AAAAGCTGAA GAGAGACTAT TTACCGAGCT AACCCTTGAA GAAAATATCA TTTTGTGGGA AAGTAGATTC TCTAGTAATG AACGGTTAGC AAGTAGCGAG TTGTTAGAGC TTACAGGTGC GCCTAAACGC TTTTTGCCTT TACTTTCACA GCCATTAGGT AAATTTTCCG GTGGAGAAAA GCAGATGATA CTGCTGGCAC TTATCATAGC TCATCCACCT AAAATATTAT TTCTAGACGA ACATACGGCA AATTTAGATC CGAAAGCTTC TTTGGAAGTG ATGAAAAAAA CAGCAGAGAT TATAGAGAAA CATAAGATCA CTTCCGTTAT GATCACGCAT AATTTAGAAG ATGCCGTAAA TTACGGCAAA AGGCTTATAG TCTTAGATAG TGGTAAGGTA GTACTAGACT ACCTAAAACC ACCGGGTTTT TCTTTAAAAG AACTTAAAGA GATTTTAAAT
Details for RicoA.10014.e.B1.GE35292
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK PYLYAENIQF KVKERTEPII AETTLNIAKE EFVVILGHNG SGKSTLAKIL AGYLKPTSGK VFLDQVRIDK IPKGQKASTL VTLTQKAEER LFTELTLEEN IILWESRFSS NERLASSELL ELTGAPKRFL PLLSQPLGKF SGGEKQMILL ALIIAHPPKI LFLDEHTANL DPKASLEVMK KTAEIIEKHK ITSVMITHNL EDAVNYGKRL IVLDSGKVVL DYLKPPGFSL KELKEILN
Validated NT Sequence
ccggntccta tcttactcac ttaatttaaa atctctttaa gttcttttaa agaaaaaccc ggtggtttta ggtagtctag tactacctta ccactatcta agactataag ccttttgccg taatttacgg catcttctaa attatgcgtg atcataacgg aagtgatctt atgtttctct ataatctctg ctgttttttt catcacttcc aaagaagctt tcggatctaa atttgccgta tgttcgtcta gaaataatat tttaggtgga tgagctatga taagtgccag cagtatcatc tgcttttctc caccggaaaa tttacctaat ggctgtgaaa gtaaaggcaa aaagcgttta ggcgcacctg taagctctaa caactcgcta cttgctaacc gttcattact agagaatcta ctttcccaca aaatgatatt ttcttcaagg gttagctcgg taaatagtct ctcttcagct ttttgtgtta atgtaactaa tgtagaagct ttttgtcctt taggtatttt atcgattctg acttgatcta gaaacacttt cccgctagta ggttttaaat agccggcaag tattttagct aaagtagact taccgctacc gttatgaccc aatattacca caaactcttc tttagcaata tttaaagtag tttcagcaat tataggttcg gttctttctt taactttgaa ctgtatattt tctgcatata aataaggttt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMK PYLYAENIQF KVKERTEPII AETTLNIAKE EFVVILGHNG SGKSTLAKIL AGYLKPTSGK VFLDQVRIDK IPKGQKASTL VTLTQKAEER LFTELTLEEN IILWESRFSS NERLASSELL ELTGAPKRFL PLLSQPLGKF SGGEKQMILL ALIIAHPPKI LFLDEHTANL DPKASLEVMK KTAEIIEKHK ITSVMITHNL EDAVNYGKRL IVLDSGKVVL DYLKPPGFSL KELKEILN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac cttatttata tgcagaaaat atacagttca aagttaaaga aagaaccgaa cctataattg ctgaaactac tttaaatatt gctaaagaag agtttgtggt aatattgggt cataacggta gcggtaagtc tactttagct aaaatacttg ccggctattt aaaacctact agcgggaaag tgtttctaga tcaagtcaga atcgataaaa tacctaaagg acaaaaagct tctacattag ttacattaac acaaaaagct gaagagagac tatttaccga gctaaccctt gaagaaaata tcattttgtg ggaaagtaga ttctctagta atgaacggtt agcaagtagc gagttgttag agcttacagg tgcgcctaaa cgctttttgc ctttactttc acagccatta ggtaaatttt ccggtggaga aaagcagatg atactgctgg cacttatcat agctcatcca cctaaaatat tatttctaga cgaacatacg gcaaatttag atccgaaagc ttctttggaa gtgatgaaaa aaacagcaga gattatagag aaacataaga tcacttccgt tatgatcacg cataatttag aagatgccgt aaattacggc aaaaggctta tagtcttaga tagtggtaag gtagtactag actacctaaa accaccgggt ttttctttaa aagaacttaa agagatttta aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for RicoA.10014.e.B1.PW35910
PURIFICATION DATe: 8/20/2012
CONCENTRATION: 24.3mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK PYLYAENIQF KVKERTEPII AETTLNIAKE EFVVILGHNG SGKSTLAKIL AGYLKPTSGK VFLDQVRIDK IPKGQKASTL VTLTQKAEER LFTELTLEEN IILWESRFSS NERLASSELL ELTGAPKRFL PLLSQPLGKF SGGEKQMILL ALIIAHPPKI LFLDEHTANL DPKASLEVMK KTAEIIEKHK ITSVMITHNL EDAVNYGKRL IVLDSGKVVL DYLKPPGFSL KELKEILN
Validated NT Sequence
ccggntccta tcttactcac ttaatttaaa atctctttaa gttcttttaa agaaaaaccc ggtggtttta ggtagtctag tactacctta ccactatcta agactataag ccttttgccg taatttacgg catcttctaa attatgcgtg atcataacgg aagtgatctt atgtttctct ataatctctg ctgttttttt catcacttcc aaagaagctt tcggatctaa atttgccgta tgttcgtcta gaaataatat tttaggtgga tgagctatga taagtgccag cagtatcatc tgcttttctc caccggaaaa tttacctaat ggctgtgaaa gtaaaggcaa aaagcgttta ggcgcacctg taagctctaa caactcgcta cttgctaacc gttcattact agagaatcta ctttcccaca aaatgatatt ttcttcaagg gttagctcgg taaatagtct ctcttcagct ttttgtgtta atgtaactaa tgtagaagct ttttgtcctt taggtatttt atcgattctg acttgatcta gaaacacttt cccgctagta ggttttaaat agccggcaag tattttagct aaagtagact taccgctacc gttatgaccc aatattacca caaactcttc tttagcaata tttaaagtag tttcagcaat tataggttcg gttctttctt taactttgaa ctgtatattt tctgcatata aataaggttt catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMK PYLYAENIQF KVKERTEPII AETTLNIAKE EFVVILGHNG SGKSTLAKIL AGYLKPTSGK VFLDQVRIDK IPKGQKASTL VTLTQKAEER LFTELTLEEN IILWESRFSS NERLASSELL ELTGAPKRFL PLLSQPLGKF SGGEKQMILL ALIIAHPPKI LFLDEHTANL DPKASLEVMK KTAEIIEKHK ITSVMITHNL EDAVNYGKRL IVLDSGKVVL DYLKPPGFSL KELKEILN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC CTTATTTATA TGCAGAAAAT ATACAGTTCA AAGTTAAAGA AAGAACCGAA CCTATAATTG CTGAAACTAC TTTAAATATT GCTAAAGAAG AGTTTGTGGT AATATTGGGT CATAACGGTA GCGGTAAGTC TACTTTAGCT AAAATACTTG CCGGCTATTT AAAACCTACT AGCGGGAAAG TGTTTCTAGA TCAAGTCAGA ATCGATAAAA TACCTAAAGG ACAAAAAGCT TCTACATTAG TTACATTAAC ACAAAAAGCT GAAGAGAGAC TATTTACCGA GCTAACCCTT GAAGAAAATA TCATTTTGTG GGAAAGTAGA TTCTCTAGTA ATGAACGGTT AGCAAGTAGC GAGTTGTTAG AGCTTACAGG TGCGCCTAAA CGCTTTTTGC CTTTACTTTC ACAGCCATTA GGTAAATTTT CCGGTGGAGA AAAGCAGATG ATACTGCTGG CACTTATCAT AGCTCATCCA CCTAAAATAT TATTTCTAGA CGAACATACG GCAAATTTAG ATCCGAAAGC TTCTTTGGAA GTGATGAAAA AAACAGCAGA GATTATAGAG AAACATAAGA TCACTTCCGT TATGATCACG CATAATTTAG AAGATGCCGT AAATTACGGC AAAAGGCTTA TAGTCTTAGA TAGTGGTAAG GTAGTACTAG ACTACCTAAA ACCACCGGGT TTTTCTTTAA AAGAACTTAA AGAGATTTTA AATTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT