SSGCID: Target Page

RicoA.10014.a

Glutamine ABC transporter ATP-binding protein

CENTER ID:	RicoA.10014.a
ORGANISM:	Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE:	Boutonneuse fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RicoA.10014.a.B1.GE35288	Full length( RicoA.10014.a )		1	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272944.4.peg.1539
RefSeq:	NP_360978.1
UniProt:	Q92FY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIILEKVCKR YGKNYAIKNI DLSFTTKETT VIIGPSGSGK TTLLRILNNL EEPSSGTFLV DGKKLLLKNR RKLLLKVGMV FQNFNLFPHL TVGENLIYTP VNVLNLPKEQ AISMAKELLA KFKLTQKFDS YPASLSGGQK QRTAIARALM MKPKILLFDE PTSALDPENI KDVIENINLL KDQMSMVVVT HHLKFAKLIA DRVIFMDQGQ ILANQPAEDF FNKPASHRAR LFLENIGDLR

NT Sequence

ATGATTATTT TAGAAAAAGT TTGTAAGCGT TATGGCAAGA ATTACGCAAT TAAGAATATT GATTTAAGTT TTACTACTAA GGAAACTACT GTAATAATCG GTCCTTCGGG AAGCGGTAAA ACTACTCTGC TTAGAATTTT AAATAATTTA GAAGAACCAA GTAGCGGTAC TTTTTTGGTA GACGGGAAGA AGCTACTGCT TAAAAATAGA AGAAAGCTTC TTTTAAAAGT TGGTATGGTT TTTCAAAATT TTAATCTATT CCCGCATTTA ACGGTAGGAG AAAACCTAAT CTATACGCCG GTAAATGTTT TAAATTTGCC AAAAGAACAG GCAATTTCTA TGGCAAAGGA ATTGCTTGCA AAATTTAAGC TAACGCAAAA ATTTGACTCA TATCCGGCAA GCCTATCAGG AGGGCAAAAG CAACGCACCG CAATAGCAAG GGCTTTAATG ATGAAGCCGA AAATTTTGTT ATTTGATGAA CCGACTTCGG CACTCGATCC TGAAAATATT AAAGATGTTA TAGAGAATAT AAATTTATTA AAAGATCAAA TGAGCATGGT AGTGGTTACG CACCATTTGA AGTTTGCAAA ATTAATAGCG GATCGTGTTA TTTTTATGGA TCAAGGACAG ATTTTAGCAA ATCAACCTGC CGAGGATTTT TTTAATAAAC CGGCATCGCA TCGAGCTAGG TTGTTTTTAG AGAATATCGG CGATTTAAGG

Details for RicoA.10014.a.B1.GE35288

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	111

Validated AA Sequence

MAHHHHHHMI ILEKVCKRYG KNYAIKNIDL SFTTKETTVI IGPSGSGKTT LLRILNNLEE PSSGTFLVDG KKLLLKNRRK LLLKVGMVFQ NFNLFPHLTV GENLIYTPVN VLNLPKEQAI SMAKELLAKF KLTQKFDSYP ASLSGGQKQR TAIARALMMK PKILLFDEPT SALDPENIKD VIENINLLKD QMSMVVVTHH LKFAKLIADR VIFMDQGQIL ANQPAEDFFN KPASHRARLF LENIGDLRXV SKIGSGXXXX XXXXXXXXXX XXXXXX

Validated NT Sequence

antttnntta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgatt attttagaaa aagtttgtaa gcgttatggc aagaattacg caattaagaa tattgattta agttttacta ctaaggaaac tactgtaata atcggtcctt cgggaagcgg taaaactact ctgcttagaa ttttaaataa tttagaagaa ccaagtagcg gtactttttt ggtagacggg aagaagctac tgcttaaaaa tagaagaaag cttcttttaa aagttggtat ggtttttcaa aattttaatc tattcccgca tttaacggta ggagaaaacc taatctatac gccggtaaat gttttaaatt tgccaaaaga acaggcaatt tctatggcaa aggaattgct tgcaaaattt aagctaacgc aaaaatttga ctcatatccg gcaagcctat caggagggca aaagcaacgc accgcaatag caagggcttt aatgatgaag ccgaaaattt tgttatttga tgaaccgact tcggcactcg atcctgaaaa tattaaagat gttatagaga atataaattt attaaaagat caaatgagca tggtagtggt tacgcaccat ttgaagtttg caaaattaat agcggatcgt gttattttta tggatcaagg acagatttta gcaaatcaac ctgccgagga tttttttaat aaaccggcat cgcatcgagc taggttgttt ttagagaata tcggcgattt aaggnaagtg agtaagatag gatccggctg nnnnnnnncn nnnnnnnnnn nnnnanntgn nnnnnnnnnn nnncnnnnnn nnnnnnnnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatta ttttagaaaa agtttgtaag cgttatggca agaattacgc aattaagaat attgatttaa gttttactac taaggaaact actgtaataa tcggtccttc gggaagcggt aaaactactc tgcttagaat tttaaataat ttagaagaac caagtagcgg tacttttttg gtagacggga agaagctact gcttaaaaat agaagaaagc ttcttttaaa agttggtatg gtttttcaaa attttaatct attcccgcat ttaacggtag gagaaaacct aatctatacg ccggtaaatg ttttaaattt gccaaaagaa caggcaattt ctatggcaaa ggaattgctt gcaaaattta agctaacgca aaaatttgac tcatatccgg caagcctatc aggagggcaa aagcaacgca ccgcaatagc aagggcttta atgatgaagc cgaaaatttt gttatttgat gaaccgactt cggcactcga tcctgaaaat attaaagatg ttatagagaa tataaattta ttaaaagatc aaatgagcat ggtagtggtt acgcaccatt tgaagtttgc aaaattaata gcggatcgtg ttatttttat ggatcaagga cagattttag caaatcaacc tgccgaggat ttttttaata aaccggcatc gcatcgagct aggttgtttt tagagaatat cggcgattta aggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat